Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Plant J ; 50(6): 1063-78, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17488239

RESUMO

As part of a larger project to sequence the Populus genome and generate genomic resources for this emerging model tree, we constructed a physical map of the Populus genome, representing one of the few such maps of an undomesticated, highly heterozygous plant species. The physical map, consisting of 2802 contigs, was constructed from fingerprinted bacterial artificial chromosome (BAC) clones. The map represents approximately 9.4-fold coverage of the Populus genome, which has been estimated from the genome sequence assembly to be 485 +/- 10 Mb in size. BAC ends were sequenced to assist long-range assembly of whole-genome shotgun sequence scaffolds and to anchor the physical map to the genome sequence. Simple sequence repeat-based markers were derived from the end sequences and used to initiate integration of the BAC and genetic maps. A total of 2411 physical map contigs, representing 97% of all clones assigned to contigs, were aligned to the sequence assembly (JGI Populus trichocarpa, version 1.0). These alignments represent a total coverage of 384 Mb (79%) of the entire poplar sequence assembly and 295 Mb (96%) of linkage group sequence assemblies. A striking result of the physical map contig alignments to the sequence assembly was the co-localization of multiple contigs across numerous regions of the 19 linkage groups. Targeted sequencing of BAC clones and genetic analysis in a small number of representative regions showed that these co-aligning contigs represent distinct haplotypes in the heterozygous individual sequenced, and revealed the nature of these haplotype sequence differences.


Assuntos
Genoma de Planta , Mapeamento Físico do Cromossomo , Populus/genética , Cromossomos Artificiais Bacterianos , Haplótipos , Repetições Minissatélites , Polimorfismo Genético , Alinhamento de Sequência , Análise de Sequência de DNA
2.
Science ; 307(5713): 1321-4, 2005 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-15653466

RESUMO

Cryptococcus neoformans is a basidiomycetous yeast ubiquitous in the environment, a model for fungal pathogenesis, and an opportunistic human pathogen of global importance. We have sequenced its approximately 20-megabase genome, which contains approximately 6500 intron-rich gene structures and encodes a transcriptome abundant in alternatively spliced and antisense messages. The genome is rich in transposons, many of which cluster at candidate centromeric regions. The presence of these transposons may drive karyotype instability and phenotypic variation. C. neoformans encodes unique genes that may contribute to its unusual virulence properties, and comparison of two phenotypically distinct strains reveals variation in gene content in addition to sequence polymorphisms between the genomes.


Assuntos
Cryptococcus neoformans/genética , Genoma Fúngico , Processamento Alternativo , Parede Celular/metabolismo , Cromossomos Fúngicos/genética , Biologia Computacional , Cryptococcus neoformans/patogenicidade , Cryptococcus neoformans/fisiologia , Elementos de DNA Transponíveis , Proteínas Fúngicas/metabolismo , Biblioteca Gênica , Genes Fúngicos , Humanos , Íntrons , Dados de Sequência Molecular , Fenótipo , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único , Polissacarídeos/metabolismo , RNA Antissenso , Análise de Sequência de DNA , Transcrição Gênica , Virulência , Fatores de Virulência/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA