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The use of flexible wearable sensors to monitor the impact of sleeping position and turning frequency on sleep and to study sleep patterns can help bedridden patients heal and recover. The flexible wearable sleeping-position monitoring device was designed and developed using a flexible angle sensor and a six-axis motion sensor to measure the dynamic changes in body posture during sleep. Based on the changes in the output parameters of the flexible angle sensor and the six-axis motion sensor, we determined the change in the subject's lying position, verifying and analyzing the relationship between lying position preference, turning frequency, and sleep quality in healthy subjects. The sleeping-position monitoring device was worn by 13 subjects (7 males and 6 females) without sleep disorders before the sleep experiment. They performed more than 50 sleeping-position changes to ensure the accuracy of the monitoring device. Subjects slept in their beds for 8 h per night for 15 nights. During that time, they wore the sleeping-position monitoring device and a wristband sleep-monitoring bracelet on their left hand, and gathered the subjective sleep data using questionnaires. The results show that the most critical influencing factors are sleeping-position preference and frequency of turning. Data analysis reveals that subjects with a preference for right-sided lying and a lower frequency of turning had better sleep quality.
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Qualidade do Sono , Transtornos do Sono-Vigília , Leitos , Feminino , Humanos , Masculino , Postura , SonoRESUMO
A glassy carbon electrode (GCE) was modified with magnetic molecularly imprinted polymers (mMIPs) using catechin as a template, reduced graphene oxide (rGO), and zeolitic imidazolate frameworks-8 (ZIF-8) for the sensitive detection of catechin (mMIPs/rGO-ZIF-8/GCE). The prepared rGO, ZIF-8, and mMIPs exhibited typical structures and properties determined by various characterizations. The mMIPs showed good selectivity for catechin among several structural analogs. The mMIPs/rGO-ZIF-8/GCE showed a higher maximum peak current for catechin than that of a single component modified GCE. After the optimization of the material ratio, coating amounts, pH, and scan rate, the mMIPs/rGO-ZIF-8/GCE exhibited good selectivity, good linearity, and a low detection limit (LOD) for catechin. The linear range was 0.01 nmol/L-10 µmol/L and the LOD was 0.003 nmol/L (S/N = 3). The relative standard deviations for reproducibility and stability tests (n = 6) were 5.2% and 6.1%, respectively. A recovery between 99.1 and 101.3% was obtained in the detection of catechin in spiked samples. Based on these findings, the proposed mMIPs/rGO-ZIF-8/GCE could be developed further, and future research could be conducted on alternate fabrication strategies and methods to create more portable and practical electrochemical sensors. Graphical Abstract.
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Catequina/análise , Grafite/química , Imidazóis/química , Polímeros Molecularmente Impressos/química , Nanocompostos/química , Zeolitas/química , Adsorção , Catequina/química , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Limite de Detecção , Reprodutibilidade dos Testes , Chá/químicaRESUMO
Consumption of α-linolenic acid (ALA)-rich flaxseed oil is a possible way to supplement ω-3 polyunsaturated fatty acids, but the beneficial effects to cardiovascular systems are still controversial. In this study, human umbilical vein endothelial cells (HUVECs) treated with 100 ng/mL lipopolysaccharide (LPS) were used as the in vitro model and the potential beneficial effects of well-characterized flaxseed oil and ALA were studied. Exposure of HUVECs to LPS for 24 h significantly promoted inflammatory response as release of interleukin 6 (IL-6), soluble intercellular cell adhesion molecule-1 (sICAM-1) and soluble vascular cell adhesion molecule 1 (sVCAM-1) as well as adhesion of THP-1 monocytes, but did not induce cytotoxicity or oxidative stress. ALA, but not flaxseed oil, significantly reduced LPS-induced release of sICAM-1 and sVCAM-1, without effect on THP-1 adhesion. No radical scavenging activity was observed after flaxseed oil or ALA exposure. Rather, ALA at high concentrations promoted intracellular superoxide associated with damages to lysosomes, which was not observed in flaxseed oil exposed cells. These results indicated that ALA at high concentrations could inhibit inflammatory responses in LPS-treated HUVECs in vitro but might also promote a modest effect in cytotoxicity and oxidative stress.
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Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Óleo de Semente do Linho/farmacologia , Lipopolissacarídeos/farmacologia , Ácido alfa-Linolênico/farmacologia , Anti-Inflamatórios/farmacologia , Células Cultivadas , Suplementos Nutricionais , Ácidos Graxos Ômega-3/metabolismo , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Interleucina-6/metabolismo , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Superóxidos/metabolismoRESUMO
Diabetic cardiomyopathy increases the risk for the development of heart failure independent of coronary artery disease and hypertension. Either type 1 or type 2 diabetes is often accompanied by varying degrees of hyperglycemia, which has been proven to induce myocardial apoptosis in animal models. Recently, a novel small molecule, ZLN005, has been reported to show antidiabetic efficacy in a mouse model, possibly by induction of PGC-1α expression. In this study, we investigated whether ZLN005 protects cardiomyocytes against high glucose-induced cytotoxicity and the mechanisms involved. Neonatal mouse cardiomyocytes were incubated with media containing 5.5 or 33mM glucose for 24h in the presence or absence of ZLN005. ZLN005 treatment led to ameliorated cardiomyocyte oxidative injury, enhanced cell viability, and reduced apoptosis in the high glucose environment. Western blot analysis revealed that high glucose suppressed cardiomyocyte autophagy, whereas ZLN005 increased the expression of autophagy marker proteins ATG5, beclin1, and LC3 II/LC3 I; this increase was accompanied by increased expression of SIRT1. Furthermore, EX527, a SIRT1-specific inhibitor, weakened the protective effects of ZLN005 on cardiomyocytes subjected to high glucose. Taken together, these results suggest that ZLN005 suppresses high glucose-induced cardiomyocyte injury by promoting SIRT1 expression and autophagy.
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Autofagia/efeitos dos fármacos , Benzimidazóis/farmacologia , Cardiotônicos/farmacologia , Glucose/toxicidade , Miócitos Cardíacos/citologia , Sirtuína 1/genética , Animais , Animais Recém-Nascidos , Apoptose/efeitos dos fármacos , Autofagia/genética , Sobrevivência Celular/efeitos dos fármacos , Citoproteção/efeitos dos fármacos , Estresse do Retículo Endoplasmático/efeitos dos fármacos , L-Lactato Desidrogenase/metabolismo , Camundongos Endogâmicos C57BL , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Estresse Oxidativo/efeitos dos fármacos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sirtuína 1/metabolismoRESUMO
A screening method using α-amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography was proposed and utilized to screen and separate α-amylase inhibitors from extract of Solanum nigrum. The α-amylase-functionalized magnetic graphene oxide was characterized and found to demonstrate satisfactory structure, magnetic response (24.5 emu/g), and reusability (retained 90% of initial activity after five cycles). The conditions for the screening with α-amylase functionalized magnetic graphene oxide were optimized and set at pH 7.0 and 25°C. As a result, two potent flavonoid compounds, apigenin-7-O-glucuronide (1) and astragalin (2), were separated and collected through high-speed counter-current chromatography and subjected to high-performance liquid chromatography analysis with purity higher than 90% (according to HPLC data), which were identified as α-amylase inhibitors. These results suggested that utilization of α-amylase functionalized magnetic graphene oxide in the rapid screening and isolation bioactive compounds from complex natural products is a feasible and environmentally friendly method.
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Inibidores Enzimáticos/isolamento & purificação , Solanum nigrum/química , alfa-Amilases/antagonistas & inibidores , Cromatografia Líquida de Alta Pressão , Distribuição Contracorrente , Grafite , Óxidos , Extratos Vegetais/químicaRESUMO
Polyphenols are plant-derived natural products with well-documented health benefits to human beings, such as antibacterial activities. However, the antibacterial activities of polyphenols under hyperglycemic conditions have been rarely studied, which could be relevant to their antibacterial efficacy in disease conditions, such as in diabetic patients. Herein, the antibacterial activities of 38 polyphenols under mimicked hyperglycemic conditions were evaluated. The structure-antibacterial activity relationships of polyphenols were also tested and analyzed. The presence of glucose apparently promoted the growth of the bacterial strains tested in this study. The OD600 values of tested bacteria strains increased from 1.09-fold to 1.49-fold by adding 800 mg/dL glucose. The polyphenols showed structurally dependent antibacterial activities, which were significantly impaired under the hyperglycemic conditions. The results from this study indicated that high blood glucose might promote bacterial infection, and the hyperglycemic conditions resulting from diabetes were likely to suppress the antibacterial benefits of polyphenols.
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Antibacterianos/química , Bactérias/crescimento & desenvolvimento , Glucose/efeitos adversos , Polifenóis/química , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Humanos , Hiperglicemia/complicações , Testes de Sensibilidade Microbiana , Modelos Teóricos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Relação Estrutura-AtividadeRESUMO
In this study, magnetic graphene oxide (MGO) nanomaterials were synthesized based on covalent binding of amino Fe3O4 nanoparticles onto the graphene oxide (GO), and the prepared MGO was successfully applied as support for the immobilization of laccase. The MGO-laccase was characterized by transmission electron microscopy (TEM) and a vibrating sample magnetometer (VSM). Compared with free laccase, the MGO-laccase exhibited better pH and thermal stabilities. The optimum pH and temperature were confirmed as pH 3.0 and 35 °C. Moreover, the MGO-laccase exhibited sufficient magnetic response and satisfied reusability after being retained by magnetic separation. The MGO-laccase maintained 59.8% activity after ten uses. MGO-laccase were finally utilized in the decolorization of dye solutions and the decolorization rate of crystal violet (CV), malachite green (MG), and brilliant green (BG) reached 94.7% of CV, 95.6% of MG, and 91.4% of BG respectively. The experimental results indicated the MGO-laccase nanomaterials had a good catalysis ability to decolorize dyes in aqueous solution. Compared with the free enzyme, the employment of MGO as enzyme immobilization support could efficiently enhance the availability and facilitate the application of laccase.
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Corantes/química , Enzimas Imobilizadas , Grafite/química , Lacase/química , Nanopartículas de Magnetita/química , Óxidos/química , Adsorção , Catálise , Ativação Enzimática , Concentração de Íons de Hidrogênio , Nanopartículas de Magnetita/ultraestrutura , TemperaturaRESUMO
A simple, rapid, organic-solvent- and sample-saving pretreatment technique, called dispersive liquid-liquid microextraction, was developed for the determination of six synthetic phenolic antioxidants from edible oils before high-performance liquid chromatography with diode array detection. The entire procedure was composed of a two-step microextraction and a centrifugal process and could be finished in about 5 min, only consuming only 25 mg of sample and 1 mL of the organic solvent for each extraction. The influences of several important parameters on the microextraction efficiency were thoroughly investigated. Recovery assays for oil samples were spiked at three concentration levels, 50, 100 and 200 mg/kg, and provided recoveries in the 86.3-102.5% range with a relative standard deviation below 3.5%. The intra-day and inter-day precisions for the analysis were less than 3.8%. The proposed method was successfully applied for the determination of synthetic phenolic antioxidants in different oil samples, and satisfactory results were obtained. Thus, the developed method represents a viable alternative for the quality control of synthetic phenolic antioxidant concentrations in edible oils.
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Antioxidantes/química , Antioxidantes/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Microextração em Fase Líquida/métodos , Óleos de Plantas/química , Antioxidantes/síntese química , Cromatografia Líquida de Alta Pressão/instrumentação , Limite de Detecção , Fenóis/químicaRESUMO
In this study, a high-speed counter-current chromatography (HSCCC) separation method target guided by centrifugal ultrafiltration with high-performance liquid chromatography-mass spectrometry (CU-LC-MS) was proposed. This method was used to analyze α-amylase inhibitors from Kadsura longipedunculata extract. According to previous screening with CU-LC-MS, two screened potential α-amylase inhibitors was successfully isolated from Kadsura longipedunculata extract using HSCCC under the optimized experimental conditions. The isolated two target compounds (with purities of 92.3% and 94.6%) were, respectively, identified as quercetin-3-O-rhamnoside (1) and protocatechuic acid (2) based on the MS, UV, and ¹H-NMR spectrometry data. To verify the inhibition of screened compounds, the inhibitory activities of quercetin-3-O-rhamnoside (1) and protocatechuic acid (2) on α-amylase were tested, and it demonstrated that the experimental IC50 values of quercetin-3-O-rhamnoside (1) and protocatechuic acid (2) were 28.8 and 12.5 µmol/L. These results proved that the hyphenated technique using CU-LC-MS and HSCCC was a rapid, competent, and reproductive method to screen and separate potential active compounds, like enzyme inhibitors from the extract of herbal medicines.
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Inibidores Enzimáticos , Hidroxibenzoatos , Kadsura/química , Quercetina , alfa-Amilases/antagonistas & inibidores , Centrifugação , Cromatografia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Hidroxibenzoatos/química , Hidroxibenzoatos/isolamento & purificação , Quercetina/análogos & derivados , Quercetina/química , Quercetina/isolamento & purificação , UltrafiltraçãoRESUMO
Radix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the n-butanol part of a Radix astragali extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the n-butanol part of a Radix astragali extract and identified as genistin (1), calycosin-7-O-ß-d-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation.
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Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Nanopartículas de Magnetita/química , 1-Butanol/química , 1-Butanol/isolamento & purificação , Astragalus propinquus , Cromatografia Líquida de Alta Pressão , Glucosídeos/química , Glucosídeos/isolamento & purificação , Isoflavonas/química , Isoflavonas/isolamento & purificação , Ligantes , Espectrometria de Massas , Soroalbumina Bovina/químicaRESUMO
Using pig manure and corn straw as raw materials for high-temperature composting, setting three different treat- ments: C/N 15, C/N 25, and C/N 35. Composting period is 120 days, which contains 30 days for ventilation cycle by forced continuous ventilation. Sampled on 0, 22, 30, 60, 90, 120th days, they were analyzed by elemental analysis and IR spectroscopy to study effect of different lignin content on compost humic acid (HA) composition and molecular structure. The results showed that the change in composting humic acid C focused on the first 30 days, while after composting, the O/C of compost HA increased, H/C decreased, and N content increased. Low C/N (15) and higher C/N ratio (35) had higher degree of oxidation than the C/N 25 in compost HA. FTIR indicated that the infrared spectrum shapes with different lignin content treatment are similar during the composting process, but the peak intensity is obviously different. Research results proved that the composting stage is more conducive to enhanced aromatic in compost HA. After composting, C/N 15 had less polysaccharide and fat ingredients and more aromatic structural components in compost HA, compared with C/N 25 and 35. In addition, compost HA of C/N 15 had higher degree of humification and its structure was more stable.
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Substâncias Húmicas/análise , Esterco , Zea mays , Animais , Lignina/química , Caules de Planta , Solo , Microbiologia do Solo , Espectroscopia de Infravermelho com Transformada de Fourier , SuínosRESUMO
Introduction: The Tan sheep is a popular local breed in China because of its tenderness and flavor. The Hu sheep breed is also famous for its high litter size, and its muscle growth rate is faster than that of Tan sheep. However, the epigenetic mechanism behind these muscle-related phenotypes is unknown. Methods: In this study, the longissimus dorsi tissue from 18 6 month-old Tan sheep, Hu sheep, and Tan-Hu F2 generation (6 sheep per population) were collected. After genomic DNA extraction, whole-genome bisulfite sequencing (WGBS) and bioinformatics analysis were performed to construct genome-wide DNA methylome maps for the Tan sheep, Hu sheep and their Tan-Hu F2 generation. Results: Distinct genome-wide DNA methylation patterns were observed between Tan sheep and Hu sheep. Moreover, DNA methylated regions were significantly increased in the skeletal muscle from Tan sheep vs. the F2 generation compared to the Hu sheep vs. F2 generation and the Tan sheep vs. Hu sheep. Compared with Hu sheep, the methylation levels of actin alpha 1 (ACTA1), myosin heavy chain 11 (MYH11), Wiskott-Aldrich syndrome protein (WAS), vav guanine nucleotide exchange factor 1 (VAV1), fibronectin 1 (FN1) and Rho-associated protein kinase 2 (ROCK2) genes were markedly distinct in the Tan sheep. Furthermore, Gene Ontology analysis indicated that these genes were involved in myotube differentiation, myotube cell development, smooth muscle cell differentiation and striated muscle cell differentiation. Conclusion: The findings from this study, in addition to data from previous research, demonstrated that the ACTA1, MYH11, WAS, VAV1, FN1, and ROCK2 genes may exert regulatory effects on muscle development.
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Different parts of Corchorus olitorius and C. capsularis possess different antioxidant compounds. This study investigated the phytochemical components and antioxidant capacities of ultrasound-assisted extraction of different plant parts of both species using spectrophotometry at various phenological stages. Results also indicate that leaves, stems and roots of C. olitorius at various growth stages showed higher phytochemical components and antioxidant potential compared to C. capsularis. The phytochemical components from roots to leaves in C. olitorius including total polyphenol 0.97-11.11 mg GAE/g DW, total flavonoid 0.99-7.78 mg QE/g DW and total tannin 4.02-26.89 TA E/g DW, whereas C. capsularis total polyphenol 1.04-7.93 mg GAE/g DW, total flavonoid 0.77-5.5.92 mg QE/g DW and total tannin content 3.17-22.73 TA E/g DW. C. olitorius produced overall 22.23%, 13.61%, 12.24% higher total polyphenol, total flavonoid and total tannin, respectively compare to C. capsularis. Different parts extract also significantly affected antioxidant capacities including DPPH, ABTS, and FRAP activity with values of 22.03-79.46% inhibition, 10.84-104.10 µmol TE/g DW, and 10.84-104.10 µmol Fe2+/g DW respectively for C. olitorius, while C. capsularis demonstrated 14.03-70.97% of DPPH inhibition, 9.16-95.60 µmol TE/g DW of ABTS and 5.31-71.82 µmol Fe2+/g DW of FRAP activity. Moreover, leaves of the flowering stage, young stems and aged roots of both species displayed a higher content of phytochemical and antioxidant activities than other growth stages. A positive correlation between the phytochemical and antioxidant potential indicated that phenolic constituents solely affected antioxidant activity. Thus, this study established that the plant's parts and phenological growth stages significantly influence the concentration of phytoconstituents and antioxidant activities, and determine the harvesting stages of the different organs of C. olitorius and C. capsularis for considerable medicinal importance as folk and industry.
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The screen-printed carbon electrode (SPCE) was one step modified with graphene (GN) and UiO-66 composites in nafion solution as a portable sensor (nafion/UiO-66/GN/SPCE) for the detection of synephrine. The used GN and UiO-66 were well-characterized, exhibiting typical structures by scanning electron microscope (SEM) with energy dispersive spectroscopy (EDS), X-ray powder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The nafion/UiO-66/GN/SPCE showed the maximum electrochemical signals for synephrine when comparing fabricated components in the cyclic voltammetric method. It was systematically investigated in modifier composition, modification volumes, pH, scan rate, and quantitative analysis ability. Under optimal conditions, the sensor exhibited a good detection limit (0.04 µmol L-1) for synephrine with a linear range of 0.5 µmol L-1 to 60 µmol L-1 (r2 = 0.995). The nafion/UiO-66/GN/SPCE had adequate reproducibility and stability with relative standard deviations lower than 2.01%. It was also applied to determine synephrine in the extract of Citrus aurantium L. with recoveries between 99.0% and 102.0%. The content of synephrine was in good agreement with that of the HPLC method. Based on its convenience and stability, the proposed nafion/UiO-66/GN/SPCE could be further developed as a portable and rapid detection sensor for natural active compounds in food, agricultural, and pharmaceutical fields.
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Salinity stress is a major environmental threat in agricultural systems. Kenaf is a promising crop for the future for cultivation in salinity-affected soils because of its high phytoremediation potential. The current study aimed to investigate the effects of salt stress using six different sodium chloride (NaCl) concentrations (0, 50, 100, 150, 200, and 250 mM) on the plant growth, physiological characteristics, bioactive constituents, and antioxidant capacity of H. cannabinus. The results indicated that the NaCl stress induced significant reductions in plant height and in the dry and fresh weights of the leaf tissue. In addition, the K, Ca, Mg, and P concentrations in this tissue also decreased under NaCl stress treatment conditions. In contrast, the NaCl stress led to the accumulation of hydrogen peroxide (H2O2), superoxide anion (O2â¢-), malondialdehyde (MDA), proline, total soluble sugar, and total soluble protein. Under NaCl stress, the levels of antioxidants, including phenolics and flavonoids, also increased. The gas chromatography-mass spectrometry (GC-MS) results showed that the volatile compounds, including heptacosane, 1-octadecanesulphonyl chloride, and tetratetracontane, were induced under the NaCl stress treatment. Furthermore, the salt stress significantly improved the antioxidant capacity of the leaf extracts. These findings may provide insight into how H. cannabinus plants respond to salt stress and may help improve its medicinal value under salt stress.
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The increasing demand of hemp (Cannabis sativa L.) has attracted more interest in exploring its phytochemical profile and bioactivities, such as anti-inflammatory effect. In this study, the phytochemicals of different hemp leaves were investigated, with the content order: total saponins content (TSC) > total alkaloids content (TAC) > total phenolics content (TPC) > total flavonoids content (TFC) > cannabinoids. Hemp leaves from Shanxi accumulated higher flavonoids and cannabinoids (i.e., THC, CBD, and CBN), while phenolics were more abundant in those from Hunan. A lipopolysaccharide (LPS)-induced inflammatory Matin-Darby canine kidney (MDCK) cell model was established to evaluate the anti-inflammatory effects of hemp leaf extracts. Hemp leaf extracts, especially the D129 and c7, significantly increased cell viability of LPS-induced inflammatory MDCK cells, and D132 significantly decreased the secretion of pro-inflammatory cytokines (TNF-α and IL-6) and the lactate dehydrogenase (LDH) activity. Except for c12, other hemp leaf extracts obviously decreased the cell morphological damage of LPS-induced inflammatory MDCK cells. The correlation analysis revealed that cannabinol (CBN) and TPC showed the strongest correlation with anti-inflammatory activities, and hierarchical clustering analysis also showed that hemp germplasms from Shanxi might be good alternatives to the common cultivar Ym7 due to their better anti-inflammatory activities. These results indicated that hemp leaves were effective in LPS-induced inflammatory MDCK cells, and flavonoids and cannabinoids were potential geographical markers for distinguishing them, which can provide new insights into the anti-inflammatory effect of hemp leaves and facilitate the application of hemp leaves as functional ingredients against inflammatory-related disorders.
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Given the rising domestic demand and increasing global prices of corn and soybean, China is looking for alternatives for these imports to produce animal fodder. Kenaf (Hibiscus cannabinus L.) has great potential as a new forage source, due to abundant proteins, phenols and flavonoids in its leaves. However, few studies have evaluated the mechanism of protein synthesis in kenaf leaves. In the current work, compared with kenaf material "L332," the percentage of crude protein content in leaves of material "Q303" increased by 6.13%; combined with transcriptome and proteome data, the kenaf samples were systematically studied to obtain mRNA-protein correlation. Then, the genes/proteins related to protein synthesis in the kenaf leaves were obtained. Moreover, this work detected mRNA expression of 20 differentially expressed genes (DEGs). Meanwhile, 20 differentially expressed proteins (DEPs) related to protein synthesis were performed parallel reaction monitoring. Fructose-1,6-bisphosphatase (FBP), nitrite reductase (NirA), prolyl tRNA synthase (PARS) and glycine dehydrogenase (GLDC) presented increased mRNA and protein levels within kenaf leaves with high protein content. Based on the obtained findings, FBP, NirA, PARS, and GLDC genes may exert a vital function in the protein synthesis of kenaf leaves. The results provide a new idea for further studying the potential genes affecting the quality trait of protein content in kenaf leaves and provide gene resources and a theoretical foundation for further cultivating high protein kenaf varieties.
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For detection of cannabidiol (CBD)-an important ingredient in Cannabis sativa L.-amino magnetic nanoparticle-decorated graphene (Fe3O4-NH2-GN) was prepared in the form of nanocomposites, and then modified on a glassy carbon electrode (GCE), resulting in a novel electrochemical sensor (Fe3O4-NH2-GN/GCE). The applied Fe3O4-NH2 nanoparticles and GN exhibited typical structures and intended surface groups through characterizations via transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray powder diffraction (XRD), vibrating sample magnetometer (VSM), and Raman spectroscopy. The Fe3O4-NH2-GN/GCE showed the maximum electrochemical signal for CBD during the comparison of fabricated components via the cyclic voltammetry method, and was systematically investigated in the composition and treatment of components, pH, scan rate, and quantitative analysis ability. Under optimal conditions, the Fe3O4-NH2-GN/GCE exhibited a good detection limit (0.04 µmol L-1) with a linear range of 0.1 µmol L-1 to 100 µmol L-1 (r2 = 0.984). In the detection of CBD in the extract of C. sativa leaves, the results of the electrochemical method using the Fe3O4-NH2-GN/GCE were in good agreement with those of the HPLC method. Based on these findings, the proposed sensor could be further developed for the portable and rapid detection of natural active compounds in the food, agricultural, and pharmaceutical fields.
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Kenaf (Hibiscus cannabinus L.) is a valuable plant with a potential health benefit because of its extensive bioactive compounds. Leaf extracts of 33 kenaf genotypes were investigated for their polysaccharide, total phenolic, and flavonoid content. The antioxidant properties were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant potential (FRAP) assays. Antimicrobial capacity was also assessed against Staphylococcus aureus and Escherichia coli using a disc diffusion assay. The polysaccharide content varied from 6.45-16.12 mg glucose per g DW. Total phenolic and flavonoid content ranged from 6.03-21.15 mg GAE/g DW and 1.55-9.24 mg RE/g DW, respectively. Similarly, varied values in the range 20.55-79.99% of inhibition by DPPH, 56.28-88.30% of inhibition by ABTS and 1.26-5.08 mmol Fe2+/g DW by FRAP assays were obtained for antioxidants of the genotype extracts. Extracts from CS4 and CS2 genotypes had the highest antioxidant activities. Kenaf leaves exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. Strong correlation was found between antioxidant activity with polysaccharide (DPPH, r = 0.893; ABTS, r = 0.819; FRAP, r = 0.864) and total phenolic content (DPPH, r = 0.850; ABTS, r = 0.959; FRAP, r = 0.953). The results suggested that the kenaf leaves could be used as a natural antioxidants and antimicrobial in food industries.
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Metal-organic frameworks (MOFs) are considered as good materials for the adsorption of many environmental pollutants. In this study, magnetic Fe3O4/MIL-88A composite was prepared by modification of MIL-88A with magnetic nanoparticles using the coprecipitation method. The structures and magnetic property of magnetic Fe3O4/MIL-88A composite were characterized and the adsorption behavior and mechanism for Bromophenol Blue (BPB) were evaluated. The results showed that magnetic Fe3O4/MIL-88A composite maintained a hexagonal rod-like structure and has good magnetic responsibility for magnetic separation (the maximum saturation magnetization was 49.8 emu/g). Moreover, the maximum adsorption amount of Fe3O4/MIL-88A composite for BPB was 167.2 mg/g and could maintain 94% of the initial adsorption amount after five cycles. The pseudo-second order kinetics and Langmuir isotherm models mostly fitted to the adsorption for BPB suggesting that chemisorption is the rate-limiting step for this monomolecular-layer adsorption. The adsorption capacity for another eight dyes (Bromocresol Green, Brilliant Green, Brilliant Crocein, Amaranth, Fuchsin Basic, Safranine T, Malachite Green and Methyl Red) were also conducted and the magnetic Fe3O4/MIL-88A composite showed good adsorption for dyes with sulfonyl groups. In conclusion, magnetic Fe3O4/MIL-88A composite could be a promising adsorbent and shows great potential for the removal of anionic dyes containing sulfonyl groups.