RESUMO
BACKGROUND AND OBJECTIVE: To study the prevalence of variable number of tandem repeats (VNTR) polymorphism in platelet membrane glycoprotein (GP) Ib alpha in a Chinese Han population and to determine the relationship between VNTR polymorphisms and aspirin resistance. METHODS: Three hundred healthy individuals and 110 patients with cerebral infarction volunteered to participate in this study. The genotype status of all participants was determined by polymerase chain reaction-restriction fragment length polymorphism analysis. Platelet aggregation in patients with cerebral infarction receiving aspirin (100 mg/day) for at least 7 days, was measured by optical transmission aggregometry. RESULTS AND DISCUSSION: Only three alleles of GP Ib alpha, namely, B, C and D, were found. Type A was not found in the Chinese Han participants. Aspirin-sensitive patients were significantly more often of CC genotype than aspirin-semi-responders. CONCLUSIONS: Only three types of alleles B, C and D were detected in the north-eastern region of China. The CC genotype of the VTNR polymorphism in GPIb appears to be more sensitive to the inhibitory action of low-dose aspirin.
Assuntos
Aspirina/uso terapêutico , Infarto Cerebral/tratamento farmacológico , Infarto Cerebral/genética , Repetições Minissatélites/genética , Inibidores da Agregação Plaquetária/uso terapêutico , Complexo Glicoproteico GPIb-IX de Plaquetas/genética , Idoso , Alelos , Infarto Cerebral/epidemiologia , China/epidemiologia , Resistência a Medicamentos , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Agregação Plaquetária/efeitos dos fármacos , Testes de Função Plaquetária , Polimorfismo Genético , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
OBJECTIVE: To evaluate the anti-cancer effect of Ailing-1 on human megakaryoblastic leukemia cells in culture. METHODS: Using living cell counting, 3H-TdR incorporation and clonal proliferation assay. RESULTS: Ailing-1 has various cytotoxicity on cell level, DNA synthesis and clone proliferation and shows good does-effect relation. The ED50 of Ailing-1 is greater than that of homoharringtonine through three different methods, consistent with the clinical result. Drug resistance has not been found. CONCLUSION: Ailing-1 is a promising anti-tumor drug.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Leucemia Megacarioblástica Aguda/patologia , DNA de Neoplasias/biossíntese , Harringtoninas/farmacologia , Mepesuccinato de Omacetaxina , Humanos , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
A randomized comparative study of levonorgestrel-releasing intrauterine device (LNG-IUD) and Norplant-2 implants was carried out in 200 women for 36 months. Three thousand one hundred woman-months of use were observed with each device. Only one pregnancy occurred in users of LNG-IUD at the 12th month of use. The change in bleeding pattern was the most frequent reason for discontinuation. The discontinuation rate for irregular bleeding with Norplant-2 was 17.3 and 26.8 at 24 and 36 months, respectively, as compared to 3.3 with LNG-IUD at both 24 and 36 months. The differences were statistically significant. Removal for amenorrhea and pain only occurred in acceptors of LNG-IUD. About 20-40% of women using Norplant-2 had prolonged bleeding through 36 months. The percentage of amenorrhea in LNG-IUD was the highest (29.3%) at the end of two years of use. More than 97% of subjects reported satisfaction with the methods used by themselves.