RESUMO
Here, a novel carrier fabricated by the interaction of negatively charged heparin and positively charged PEI and Ca2+ was investigated to deliver AIB1 siRNA into breast cancer cells both in vitro and in vivo. Ca2+/PEI/heparin nanoparticles were prepared by simply mixing heparin, PEI and CaCl2 aqueous solution. Heparin in the Ca2+/PEI/heparin nanoparticles (40.9% heparin, w/w) decreased the cytotoxicity of PEI. According to the MTT assay, Ca2+/PEI/heparin NPs are superior to commercial Lipofectamine 2000 considering the safety. The Ca2+/PEI/heparin NPs are able to deliver siAIB1 into breast cancer cells as effectively as Lipofectamine 2000 both in vitro and in vivo. The in vivo experiment also indicated that the NF-κB/BCL-2 signal pathway might be the downstream signal pathway of AIB1 in regulating breast cancer proliferation and progression.
RESUMO
56 essential hypertension patients (stages I, II) received Qigong from Mar, 1989 to Sept, 1991, pre- and post-treatment comparison of BP and life quality (LQ) indices revealed that (1) It could efficiently lower BP(P < 0.001). Stroke, congestive heart failure and acute myocardial infarction did not occur. No side effects were observed as well. (2) It could markedly improve LQ of hypertensive patients. LQ examinations showed that significant difference (P < 0.001) existed among LQ indices except dysfunction, which might be due to the selection of non-dysfunctional patients. LQ should be widely used in clinical practice and medical research.