LncRNA WTAPP1 promotes cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1.

BACKGROUND: Long non-coding RNA (lncRNA) Wilms Tumor 1 Associated Protein Pseudogene 1 (WTAPP1) has been reported to be a critical player in the angiogenesis and migration of endothelial progenitor cells, while its involvement in cancer biology remains unknown. This study was carried out to investigate the role of WTAPP1 in non-small cell lung cancer (NSCLC). METHODS: The expression of WTAPP1 and lncRNA HAND2 Antisense RNA 1 (HAND2-AS1) in plasma and tissues from NSCLC patients was detected by qRT-PCR. A 5-year follow-up study was carried out to explore the prognostic value of WTAPP1 for NSCLC. Overexpression experiments were performed to analyze the interaction between WTAPP1 and HAND2-AS1. Cell invasion and migration were evaluated by Transwell assays. RESULTS: The expression of WTAPP1 was upregulated in NSCLC. The survival analysis showed that low plasma levels of WTAPP1 were accompanied with high survival rate. HAND2-AS1 was downregulated in NSCLC and inversely correlated with WTAPP1 across tumor tissues. Overexpression of WTAPP1 resulted in downregulation of HAND2-AS1 in NSCLC cells, while overexpression of HAND2-AS1 did not affect the expression of WTAPP1. Overexpression of WTAPP1 led to promoted, while overexpression of HAND2-AS1 resulted in inhibited invasion and migration of NSCLC cells. In addition, overexpression of HAND2-AS1 partially attenuated the effects of overexpressing WTAPP1. In addition, WTAPP1 did not affect cancer cell proliferation. CONCLUSION: WTAPP1 may promote cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1.

Fluidization-melting characteristics of fly ash from municipal solid waste incineration.

Fly ash (FA) from municipal solid waste incineration contains hazardous substances such as dioxins, furans, and heavy metals. Melting FA has proved to be an effective method for reducing volume and mass, while also rendering the waste harmless. However, during the melting process, the addition of a fluxing agent with calorific value is currently necessary to increase melting capacity and reduce energy consumption, which presents a challenge. To tackle this issue, a fluidization-melting technology for a fuel/FA mixture is proposed, wherein a fuel source is employed in the melting process, producing ash that can serve as a fluxing agent. To test this approach, rice husk (RH) was utilized as fuel in a small-scale fluidization-melting test. The objective of this study was to examine the operation parameters of the platform and the characteristics of the resulting product, and to evaluate the harm reduction effect of the slag and its potential for resource utilization. The operating temperature was set at 690 °C in the thermal modification unit and at 1450 °C in the melting furnace, resulting in stable operation and continuous liquid slag discharge. The leaching toxicity of heavy metals in the obtained slag was lower than the standard limit, achieving harmless disposal of FA. However, the resource utilization potential of the obtained slag is limited due to its failure to meet the criteria of vitrified substance and environmental quality requirements. These limitations could be addressed by promoting the combustion of carbon in the melting furnace and accelerating the cooling rate of the slag in the quenching unit.

Self-assembling peptide hydrogel scaffold integrating stem cell-derived exosomes for infected bone defects.

Infected bone defect (IBD) is a great challenge in orthopedics, which involves in bone loss and infection. Here, a self-assembling hydrogel scaffold (named AMP-RAD/EXO), integrating antimicrobial peptides(AMPs), RADA16 and BMSCs exosomes with an innovative strategy, is developed and applied in IBD treatment for sustained antimicrobial ability, accelerating osteoblasts proliferation and promoting bone regeneration. AMPs present an excellent ability to inhibit infection, RADA16 is a self-assembling peptide hydrogel for AMPs delivery, and BMSCs exosomes can promote the bone regeneration. The prepared AMP-RAD/EXO exhibited a polyporous 3D structure for imbibition of BMSCs exosomes and migration of osteoblasts. In vitro studies indicate AMP-RAD/EXO can inhibit the growth of Staphylococcus aureus, accelerate the proliferation and migration of BMSCs. More importantly, in vivo results also prove that AMP-RAD/EXO exhibit an excellent effect on IBD treatment. Thus, the prepared AMP-RAD/EXO provides a multifunctional scaffold concept for bone tissue engineering technology.

Distal aortic arch dysplasia accompanied by multiple aneurysms in adult: A case report and review of the literature.

Because of the high blood flow and high pressure, the aortic arch, which sends out 3 branches including the innominate artery that separates the right common carotid artery and the right subclavian artery, the left common carotid artery, and the left subclavian artery, is an important blood vessel supplying the brain and spinal cord, so the risk of aneurysms in this area is very high. The main risk is that the rupture can cause massive bleeding; the secondary is that some aortic arch lesions can cause insufficient blood supply to the brain or spinal cord. If early diagnosis can be detected, it could be treated completely with minimally invasive or open surgery. Therefore, especially for this kind of patient, the significance of treatment is great, and better results can be obtained through treatment. Here is a 48-year-old female patient with dysplasia of the distal aortic arch, multiple aortic parietal aneurysms, and abnormal origin of the left subclavian artery, which originates from the tortuous and twisted aorta.

The role of lactoferrin in bone remodeling: evaluation of its potential in targeted delivery and treatment of metabolic bone diseases and orthopedic conditions.

Lactoferrin (Lf) is a multifunctional protein that is synthesized endogenously and has various biological roles including immunological regulation, antibacterial, antiviral, and anticancer properties. Recently, research has uncovered Lf's critical functions in bone remodeling, where it regulates the function of osteoblasts, chondrocytes, osteoclasts, and mesenchymal stem cells. The signaling pathways involved in Lf's signaling in osteoblasts include (low density lipoprotein receptor-related protein - 1 (LRP-1), transforming growth factor ß (TGF-ß), and insulin-like growth factor - 1 (IGF-1), which activate downstream pathways such as ERK, PI3K/Akt, and NF-κB. These pathways collectively stimulate osteoblast proliferation, differentiation, and mineralization while inhibiting osteoclast differentiation and activity. Additionally, Lf's inhibitory effect on nuclear factor kappa B (NF-κB) suppresses the formation and activity of osteoclasts directly. Lf also promotes chondroprogenitor proliferation and differentiation to chondrocytes by activating the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and phosphoinositide 3-kinase/protein kinase B(PI3K/Akt)signaling pathways while inhibiting the expression of matrix-degrading enzymes through the suppression of the NF-κB pathway. Lf's ability to stimulate osteoblast and chondrocyte activity and inhibit osteoclast function accelerates fracture repair, as demonstrated by its effectiveness in animal models of critical-sized long bone defects. Moreover, studies have indicated that Lf can rescue dysregulated bone remodeling in osteoporotic conditions by stimulating bone formation and suppressing bone resorption. These beneficial effects of Lf on bone health have led to its exploration in nutraceutical and pharmaceutical applications. However, due to the large size of Lf, small bioactive peptides are preferred for pharmaceutical applications. These peptides have been shown to promote bone fracture repair and reverse osteoporosis in animal studies, indicating their potential as therapeutic agents for bone-related diseases. Nonetheless, the active concentration of Lf in serum may not be sufficient at the site requiring bone regeneration, necessitating the development of various delivery strategies to enhance Lf's bioavailability and target its active concentration to the site requiring bone regeneration. This review provides a critical discussion of the issues mentioned above, providing insight into the roles of Lf in bone remodeling and the potential use of Lf as a therapeutic target for bone disorders.

The construction of nursing performance evaluation model in community health service center based on the balanced scorecard and hygiene factors.

To develop and design a nursing performance evaluation model for community health service centers for nursing performance management. Preliminary evaluation indicators were constructed through literature retrieval and research. Applying the Delphi method, 20 experts were invited to conduct two rounds of questionnaire consultation and indicator importance scoring. The primary indicators use the Delphi method to determine the weights, and the secondary indicators use the AHP method to determine the weights. The nursing unit evaluation model (including 30 indicators), the nursing staff evaluation model (including 21 indicators), and the performance evaluation hygiene factors model (including 5 indicators) were constructed. The recovery rate of the expert questionnaire was 100%, and the authority coefficient was 0.768. The degree of coordination was in line with the standard, and the consistency of the judgment matrix of the analytic hierarchy process was acceptable. The model is scientific and innovative, which adapts to the work characteristics and development needs of community health service centers, and provides a practical tool for nursing performance evaluation.

Effect of Huaiqihuang Granules Combined with Comprehensive Nursing on Children with Primary Nephrotic Syndrome.

BACKGROUND: To observe the effect of Huaiqihuang granules combined with comprehensive nursing intervention on children with primary nephrotic syndrome (PNS) and its effect on renal function index. METHODS: A total of 104 patients were included, and the patients were randomly divided into two groups, with 52 cases in each group. The control group was treated with glucocorticoid, and the study group was treated with Huaiqihuang granules. The clinical efficacy of the two groups was observed. The levels of TG, TC, EGFR, 24 h UTP, BUN, Scr, IgA, IgG, IgM, IFN-γ and TNF-α were compared between two groups before and after treatment. The incidence of adverse reactions and recurrence rate after treatment were compared between the two groups. RESULTS: The effective rate of the study group (94.23%) was significantly higher than that of the control group (78.85%). Before treatment, there was no significant difference in TG and TC levels between the two groups. After treatment, the levels of TG and TC in both groups were decreased, and the decrease was more obvious in the study group. Compared with before treatment, the levels of 24 h UTP, BUN, Scr, IFN-γ, and TNF-α in both groups were significantly decreased after treatment, while EGFR, IgA, IgG, and IgM levels were significantly increased. Compared with the control group, the changes of each index in the study group were more obvious after treatment. After treatment, the incidence of adverse reactions and recurrence rate in the study group were significantly lower than those in the control group. CONCLUSIONS: Huaiqihuang granules combined with comprehensive nursing treatment in children with PNS can reduce the occurrence of recent recurrence and adverse reactions and improve the cellular immune function and renal function.

A prognostic signature based on cuprotosis-related long non-coding RNAs predicts the prognosis and sensitivity to chemotherapy in patients with colorectal cancer.

Cuprotosis, a newly proposed mechanism of cell death, can trigger acute oxidative stress that leads to cell death by mediating protein lipidation in the tricarboxylic acid cycle. However, cuprotosis-related long non-coding RNAs (CRLNCs) and their relationship with prognosis and the immunological landscape of colorectal cancer (CRC) are unclear. We have developed a lncRNA signature to predict survival time, immune infiltration, and sensitivity to chemotherapy. CRLNCs were screened using the Cor function of the R software and the differentially expressed lncRNAs were collected with the limma package. Differentially expressed long non-coding RNAs (lncRNAs) associated with prognosis were selected using univariate regression analysis. A prognostic signature was developed using the least absolute shrinkage and selection operator (LASSO) and multivariate regression analysis. Patients with CRC were divided into two groups based on the risk score. The low-risk group had a more favorable prognosis, higher expression of immune checkpoints, and a higher level of immune cell infiltration compared with the high-risk group. Furthermore, there was a close association between the risk score and the clinical stage, tumor mutational burden, cancer stem cell index, and microsatellite instability. We also assessed chemotherapy response in the two risk groups. Our study analyzed the role of CRLNCs in CRC and provided novel targets and strategies for CRC chemotherapy and immunotherapy.

Fast-track surgery nursing intervention in CRC patients with laparotomy and laparoscopic surgery.

In this study, the application effect of fast-track surgery (FTS) nursing intervention in laparotomy and laparoscopic surgery for colorectal cancer (CRC) is investigated, and the optimal perioperative management strategy for CRC surgery is explored. One hundred thirty CRC patients are included in this study, in which 67 patients undergo laparotomy (Group A) and 63 patients undergo laparoscopic surgery (Group B). These patients were also randomly divided into traditional nursing subgroup (Group A1 [n = 33] and Group B1 [n = 31]) and FTS nursing subgroup (Group A2 [n = 34] and Group B2 [n = 32]). The general data of patients, pre-operative preparation, intra-operative data, postoperative recovery data, and postoperative complications are recorded. Both FTS and laparoscopic surgery can advance the anal exhaust time, and shorten postoperative fasting and water deprivation time, and the hospitalization time without increasing the incidence of complications. FTS has advantages in reducing the indwelling time of gastric tube and throat pain. Simultaneous implementation of FTS and laparoscopic surgery has the best effect on the postoperative recovery of CRC patients.

[Retracted] MicroRNA­338­3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo.

Following the publication of the above article, the authors have requested that it be retracted. After having repeated some of the experiments, the authors were not able to reproduce certain of the results. Furthermore, following a further investigation in the Editorial Office, it came to light that some of the wstern blotting data shown in Fig. 3 and the tumor images in Fig. 5 were strikingly similar to those that had been submitted for publication prior to the receipt of present article. Therefore, this article has been retracted from the Journal; all the authors agree to this retraction. The Editor and the authors would like to apologize for any inconvenience caused. [the original article was published in Molecular Medicine Reports 12: 3951­3957, 2015; DOI: 10.3892/mmr.2015.3820].

MicroRNA-338-3p suppresses tumor growth of esophageal squamous cell carcinoma in vitro and in vivo.

Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal squamous cell carcinoma (ESCC) and are crucial in tumorigenesis, among which miR­338­3p has been examined to be downregulated in patients with ESCC. However, the role of miR­338­3p in ESCC remains to be elucidated. In the present study, the role of miR­338­3p on the growth and survival of an ESCC cell line was determined with several in vitro approaches and in nude mouse models. It was determined that miR­338­3p expression was frequently downregulated in ESCC tissue compared with corresponding adjacent non­tumor tissue, and that its expression was significantly correlated with tumor stage and metastasis. Overexpression of miR­338­3p in ESCC cells suppressed cell proliferation, colony formation, migration and invasion, and induced cell arrest at the G0/G1 stage and cell apoptosis in vitro. In addition, it was demonstrated that overexpression of miR­338­3p significantly suppresses tumor growth of xenograft tumors in mice (P<0.05). These findings revealed that miR­338­3p may act as a tumor suppressor in ESCC, and its dysregulation may be involved in the initiation and development of human ESCC. In addition, it was suggested that miR­338­3p may be a potential therapeutic agent for treatment of ESCC.