Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 50
Filtrar
1.
J Nat Prod ; 87(9): 2327-2334, 2024 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-39258410

RESUMO

Two sulfur-containing heterodimers of a cytochalasan and a macrolide, sucurchalasins A and B (1 and 2), and four known cytochalasan monomers (3-6), as well as four known macrolide derivatives (7-10), were obtained from the endophytic fungus Aspergillus spelaeus GDGJ-286. Sucurchalasins A and B (1 and 2) are the first cytochalasan heterodimers formed via a thioether bridge between cytochalasan and curvularin macrolide units. Their structures were elucidated by detailed analysis of NMR, LC-MS/MS, and X-ray crystallography. In bioassays, 1 and 2 exhibited cytotoxic effects on A2780 cells, with IC50 values of 3.9 and 8.3 µM, respectively. They also showed antibacterial activities against E. faecalis and B. subtilis with MIC values of 3.1 and 6.3 µg/mL, respectively.


Assuntos
Aspergillus , Citocalasinas , Macrolídeos , Aspergillus/química , Citocalasinas/farmacologia , Citocalasinas/química , Citocalasinas/isolamento & purificação , Macrolídeos/farmacologia , Macrolídeos/química , Estrutura Molecular , Humanos , Testes de Sensibilidade Microbiana , Antibacterianos/farmacologia , Antibacterianos/química , Enxofre/química , Cristalografia por Raios X , Bacillus subtilis/efeitos dos fármacos
2.
Bioorg Chem ; 151: 107683, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39121595

RESUMO

Eighteen new oleanane-type triterpenoids were isolated from the stems of Sabia limoniacea, including sabialimon A (1), a triterpenoid with an unprecedented 6/6/6/7/7 pentacyclic skeleton and seventeen undescribed triterpenoids, sabialimons B-R (2 - 18), along with six previously described analogs (19 - 24). Their structures were fully elucidated via extensive spectroscopic analysis including 1D and 2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), experimental electronic circular dichroism measurements and X-ray crystallographic studies. Compound 1 is the first triterpenoid that possesses a rare ring system (6/6/6/7/7) with an oxygen-bearing bridge between C-17 and C-18 and a hemiketal form at C-17, which is generated a larger ring by the degradation of C-28 and D/E-ring expansion. Biological evaluation revealed that sabialimon I (9), sabialimon K (11), sabialimon P (16) and 11,13(18)-oleanadien-28-hydroxymethyl 3-one (20) exhibited significantly inhibitory activities against nitric oxide (NO) release with IC50 values of 29.65, 23.41, 18.12 and 26.64 µM, respectively, as compared with the positive control (dexamethasone, IC50 value: 40.35 µM). Furthermore, sabialimon P markedly decreased the secretion of TNF-α, iNOS, IL-6 and NF-κB and inhibited the expression of COX-2 and NF-κB/p65 in LPS-induced RAW264.7 cells in a dose-dependent manner.


Assuntos
Ácido Oleanólico , Camundongos , Animais , Células RAW 264.7 , Ácido Oleanólico/farmacologia , Ácido Oleanólico/química , Ácido Oleanólico/isolamento & purificação , Ácido Oleanólico/análogos & derivados , Estrutura Molecular , Relação Estrutura-Atividade , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Óxido Nítrico/metabolismo , Lipopolissacarídeos/farmacologia , Lipopolissacarídeos/antagonistas & inibidores , Relação Dose-Resposta a Droga , Anti-Inflamatórios não Esteroides/farmacologia , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Óxido Nítrico Sintase Tipo II/antagonistas & inibidores , Óxido Nítrico Sintase Tipo II/metabolismo , Ciclo-Oxigenase 2/metabolismo
3.
Mar Drugs ; 22(10)2024 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-39452883

RESUMO

Six new highly oxidized seco-terpenoids, including three 3-nor-labdane type diterpenes, talaroterpenoids A-C (1-3), and three meroterpenoids containing an orthoester group, talaroterpenoids D-F (6-8), together with five known compounds (4-5 and 9-11), were isolated from the marine-derived fungus Talaromyces aurantiacus. Their chemical structures were elucidated through 1D, 2D NMR, HRESIMS, J-based configuration analysis (JBCA), computational ECD calculations, and single-crystal X-ray diffraction analysis. Compounds 1 and 2 contain an unusual 6,20-γ-lactone-bridged scaffold. Compounds 10 and 11 presented inhibitory effects on NO release in lipopolysaccharide (LPS)-induced BV-2 cells with IC50 values of 11.47 and 11.32 µM, respectively. Talaroterpenoid C (3) showed moderate antifungal activity against A. alternata and P. theae Steyaert.


Assuntos
Talaromyces , Talaromyces/química , Animais , Terpenos/farmacologia , Terpenos/química , Terpenos/isolamento & purificação , Antifúngicos/farmacologia , Antifúngicos/química , Antifúngicos/isolamento & purificação , Camundongos , Organismos Aquáticos , Estrutura Molecular , Linhagem Celular , Óxido Nítrico/metabolismo , Cristalografia por Raios X
4.
Molecules ; 29(2)2024 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-38257261

RESUMO

Chemical investigation of Penicillium sp. GDGJ-N37, a Sophora tonkinensis-associated fungus, yielded two new azaphilone derivatives, N-isoamylsclerotiorinamine (1) and 7-methoxyl-N-isoamylsclerotiorinamine (2), and four known azaphilones (3-6), together with two new chromone derivatives, penithochromones X and Y (7 and 8). Their structures were elucidated based on spectroscopic data, CD spectrum, and semi-synthesis. Sclerotioramine (3) showed significant antibacterial activities against B. subtilis and S. dysentery, and it also showed most potent anti-plant pathogenic fungi activities against P. theae, C. miyabeanus, and E. turcicum.


Assuntos
Anti-Infecciosos , Penicillium , Sophora , Anti-Infecciosos/farmacologia , Antibacterianos/farmacologia , Fungos
5.
Molecules ; 28(8)2023 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-37110527

RESUMO

Four new dammarane triterpenoid saponins cypaliurusides Z1-Z4 (1-4) and eight known analogs (5-12) were isolated from the leaves of Cyclocarya paliurus. The structures of the isolated compounds were determined using a comprehensive analysis of 1D and 2D NMR and HRESIMS data. The docking study demonstrated that compound 10 strongly bonded with PTP1B (a potential drug target for the treatment of type-II diabetes and obesity), hydrogen bonds, and hydrophobic interactions, verifying the importance of sugar unit. The effects of the isolates on insulin-stimulated glucose uptake in 3T3-L1 adipocytes were evaluated and three dammarane triterpenoid saponins (6, 7 and 10) were found to enhance insulin-stimulated glucose uptake in 3T3-L1 adipocytes. Furthermore, compounds 6, 7, and 10 exhibited potent abilities to promote insulin-stimulated glucose uptake in 3T3-L1 adipocytes in a dose-dependent manner. Thus, the abundant dammarane triterpenoid saponins from C. paliurus leaves exhibited stimulatory effects on glucose uptake with application potential as a antidiabetic treatment.


Assuntos
Saponinas , Triterpenos , Animais , Camundongos , Células 3T3-L1 , Triterpenos/química , Insulina/farmacologia , Glucose/análise , Saponinas/química , Folhas de Planta/química , Adipócitos , Damaranos
6.
Bioorg Chem ; 111: 104847, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33798849

RESUMO

Diabetes mellitus is caused by chronic inflammation and affects millions of people worldwide. Cyclocarya paliurus leaves have been widely used in traditional folk tea as a remedy for diabetes, but the antidiabetic constituents remain to be further studied. The α-glucosidase inhibitory and anti-inflammatory activities were examined to evaluate their effects on diabetes mellitus, and bioassay-guided separation of C. paliurus leaves led to the identification of twenty dammarane saponins, including eleven new dammarane saponins (1-11). The structures of the isolates were elucidated by spectroscopic methods. Bioactivity assay results showed that compounds 1 and 2 strongly inhibited α-glucosidase activity, with IC50 values ranging from 257.74 µM, 282.23 µM, and strongly inhibited the release of NO, with IC50 values of 9.10 µM, 9.02 µM. Moreover, compound 2 significantly downregulated the mRNA expression of iNOS, COX-2, IL-1ß, NF-κB, IL-6 and TNF-α in LPS-mediated RAW 264.7 cells and markedly suppressed the protein expression of iNOS, NF-κB/p65, and COX-2. Dammarane glucoside 2 exhibited the strongest α-glucosidase inhibitory and anti-inflammatory activities. In addition, the structure-activity relationships (SARs) of the dammarane saponins were investigated. In summary, C. paliurus leaves showed marked α-glucosidase inhibitory and anti-inflammatory activities, and dammarane saponins are responsible for regulating α-glucosidase, inflammatory mediators, and mRNA and the protein expression of proinflammatory cytokines, which could be meaningful for discovering new antidiabetic agents.


Assuntos
Anti-Inflamatórios/farmacologia , Citocinas/antagonistas & inibidores , Inibidores de Glicosídeo Hidrolases/farmacologia , Juglandaceae/química , Triterpenos/farmacologia , alfa-Glucosidases/metabolismo , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Citocinas/genética , Relação Dose-Resposta a Droga , Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/isolamento & purificação , Camundongos , Estrutura Molecular , Folhas de Planta/química , Células RAW 264.7 , Relação Estrutura-Atividade , Triterpenos/química , Triterpenos/isolamento & purificação , Damaranos
7.
Mar Drugs ; 20(1)2021 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-35049860

RESUMO

Four undescribed compounds, guhypoxylonols A (1), B (2), C (3), and D (4), were isolated from the mangrove endophytic fungus Aspergillus sp. GXNU-Y45, together with seven previously reported metabolites. The structures of 1-4 were elucidated based on analysis of HRESIMS and NMR spectroscopic data. The absolute configurations of the stereogenic carbons in 1-3 were established through a combination of spectroscopic data and electronic circular dichroism (ECD). Compounds 1-11 were evaluated for their anti-inflammatory activity. Compounds 1, 3, 4, and 6 showed an inhibitory activity against the production of nitric oxide (NO), with the IC50 values of 14.42 ± 0.11, 18.03 ± 0.14, 16.66 ± 0.21, and 21.05 ± 0.13 µM, respectively.


Assuntos
Acanthaceae , Anti-Inflamatórios/farmacologia , Aspergillus , Óxido Nítrico/metabolismo , Policetídeos/farmacologia , Animais , Anti-Inflamatórios/química , Organismos Aquáticos , Camundongos , Folhas de Planta/microbiologia , Policetídeos/química , Células RAW 264.7
8.
Chem Biodivers ; 16(5): e1800465, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30779297

RESUMO

Five prenylflavonoids, 6-prenylnaringenin (1), 8-prenylnaringenin (2), 7-O-methyl-8-prenylnaringenin (3), 7-O-methyl-6-prenylnaringenin (4), and 4'-O-methyl-6-prenylnaringenin (5), were isolated from the traditional herb Mallotus conspurcatus Croizat (Euphorbiaceae). Compounds 1-5 revealed cytotoxic activity against cervical cancer (HeLa) cells with IC50 values ranging from 10.08 to 60.16 µm by MTT method, and interestingly, these prenylflavonoids were less toxic to normal HL-7702 cells. Furthermore, compounds 1 and 5 could inhibit the c-myc expression and telomerase activity and cause mitochondrial dysfunction. These findings might contribute to a better understanding of the biological activities of prenylflavonoids and lay the foundation for further studies on the cytotoxic activity of natural products isolated from M. conspurcatus.


Assuntos
Flavonoides/química , Mallotus (Planta)/química , Apoptose/efeitos dos fármacos , Caspase 3/metabolismo , Linhagem Celular , Regulação para Baixo , Flavanonas/química , Flavanonas/isolamento & purificação , Flavanonas/farmacologia , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Células HeLa , Humanos , Mallotus (Planta)/metabolismo , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Extratos Vegetais/química , Raízes de Plantas/química , Raízes de Plantas/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Telomerase/metabolismo
9.
J Nat Prod ; 79(10): 2472-2478, 2016 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-27704822

RESUMO

A pair of enantiomers and a pair of 2,3-dihydro-1H-indene epimers, rac-indidene A (rac-1), indidenes B and C (2, 3); four new coumarin glucosides (4-7); and four known coumarin glucosides (8-11) were isolated from the bark of Streblus indicus (Bur.) Corner. The structures of 1-11 were defined by physical data analyses, including MS, NMR, and single-crystal X-ray diffraction. The absolute configurations of the 2,3-dihydro-1H-indene derivatives were defined via experimental and calculated ECD data. rac-Indidene A and indidenes B and C showed inhibitory activity against A549 and MCF-7 tumor cells with IC50 values in the range of 2.2 ± 0.1 to 7.2 ± 0.9 µM.


Assuntos
Cumarínicos/isolamento & purificação , Cumarínicos/farmacologia , Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/farmacologia , Glucosídeos/isolamento & purificação , Glucosídeos/farmacologia , Indenos/isolamento & purificação , Indenos/farmacologia , Moraceae/química , Casca de Planta/química , Células A549 , Cromatografia Líquida de Alta Pressão , Cumarínicos/química , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Glucosídeos/química , Humanos , Indenos/química , Concentração Inibidora 50 , Células MCF-7 , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Estereoisomerismo
10.
Mar Drugs ; 14(3)2016 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-27005638

RESUMO

One new hydroanthraquinone dimer with a rare C-9-C-7' linkage, nigrodiquinone A (1), and four known anthraquinone monomers 2-5, were isolated from a fungus Nigrospora sp. obtained from the zoanthid Palythoa haddoni collected in the South China Sea. The structure of 1 was established through extensive NMR spectroscopy, and the absolute configuration was elucidated by comparing computed electronic circular dichroism (ECD) and optical rotations (OR) with experimental results. All the compounds were evaluated for antiviral activity, and 1 was also evaluated for antibacterial activity. Compound 4 displayed mild antiviral activity against coxsackie virus (Cox-B3) with the IC50 value of 93.7 µM, and 5 showed an IC50 value of 74.0 µM against respiratory syncytial virus (RSV).


Assuntos
Antraquinonas/isolamento & purificação , Antibacterianos/isolamento & purificação , Antivirais/isolamento & purificação , Ascomicetos/química , Antraquinonas/química , Antraquinonas/farmacologia , Antibacterianos/química , Antibacterianos/farmacologia , Antivirais/química , Antivirais/farmacologia , China , Dicroísmo Circular , Concentração Inibidora 50 , Espectroscopia de Ressonância Magnética , Oceanos e Mares , Rotação Ocular
11.
Rapid Commun Mass Spectrom ; 28(21): 2363-70, 2014 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-25279750

RESUMO

RATIONALE: An unambiguous identification of compounds can be achieved by comparison of known fragmentation patterns. While the literature about fragmentation mechanisms of lignans, flavonoids and triterpenoids is few. So the present study analyses the fragmentation mechanisms of these compounds isolated from Streblus asper. METHODS: Electrospray ionization ion trap mass spectrometry (ESI-ITMS) and atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS) were used to obtain the MS(n) spectra of the compounds. By analyzing the differences between the ions, the fragmentation mechanisms of these compounds were explored. RESULTS: Of the 29 compounds detected, 17, 7, and 5 were lignans, flavonoids and triterpenoids, respectively. The majority of lignans were found to give [M - H](-) ions of sufficient abundance for MS(n) analyses. The flavonoids were prone to the loss of CO and H2O. The triterpenoids always lost one formic acid molecule and two hydrogens, or one H2O from [M - H](-) to form the most abundant product ion in the MS(n) spectrum. CONCLUSIONS: ESI/APCI-ITMS were demonstrated to be fast, effective and practical tools to characterize the structures of flavonoids, triterpenoids and lignans. Results of the present study can help identify the analogous constituents by analyzing their MS(n) spectra.


Assuntos
Flavonoides/química , Lignanas/química , Moraceae/química , Espectrometria de Massas em Tandem/métodos , Triterpenos/química , Flavonoides/análise , Lignanas/análise , Metanol , Extratos Vegetais/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Triterpenos/análise
12.
Nat Prod Res ; : 1-6, 2024 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-39374371

RESUMO

Two new polyketones, exserone B (1) and cytosporone F (2), along with three known metabolites, were isolated from the mangrove endophytic fungus Aspergillus TH4b. The structures of 1 and 2 were determined by detailed NMR, and MS spectroscopic data. The absolute configurations of 1 and 2 were determined by single crystal X-ray diffraction analysis and the combination of experimental ECD and computational ECD, respectively. Compounds 1-2 have strong inhibitory activity against citrus Psyllid (Diaphorina citri) with 95.4% and 93.7% lethal at 1000 mg/kg.

13.
Fitoterapia ; 179: 106253, 2024 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-39396653

RESUMO

Phytochemical investigation of the stems of Mallotus paxii Pamp. led to the isolation and identification of four new compounds, including three neolignans (1-3) and one phenol (13), along with eight known neolignans (4-12) and one known phenol (14). Their structures were determined by spectroscopic methods, including NMR, MS and ECD analyses. Bioassay demonstrated that malloapelin A (4) exhibited a most potent anti-inflammatory activity to NO release with IC50 value of 21.32 µM. Furthermore, malloapelin A (4) markedly decreased the secretion of TNF-α, iNOS, and NF-κB and inhibited the expression of COX-2 and NF-κB/p65 in LPS-induced RAW264.7 cells in a dose-dependent manner.

14.
Bioorg Med Chem Lett ; 23(7): 2238-44, 2013 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-23434030

RESUMO

Four new lignans, strebluslignanol F (1), (7'R,8'S,7″R,8″S)-erythro-strebluslignanol G (2), isomagnaldehyde (3) and isostrebluslignanaldehyde (4), along with 12 known lignans (5-16) were isolated from the ethyl acetate-soluble part of MeOH extract of the root of Streblus asper. Their structures were elucidated through various spectroscopic methods, including 1D NMR ((1)H NMR, (13)C NMR), 2D NMR (HMQC, HMBC and NOESY) and HRMS. The stereochemistry at the chiral centers was determined using CD spectra, as well as analyses of coupling constants and optical rotation data. The isolated lignans were evaluated for their anti-HBV activities in vitro using the HBV transfected HepG2.2.15 cell line. The most active lignans, (7'R,8'S,7″R,8″S)-erythro-strebluslignanol G, magnolol, isomagnolol and isolariciresinol, exhibited significant anti-HBV activities with IC50 values of 1.58, 2.03, 10.34 and 3.67 µM, respectively, for HBsAg with no cytotoxicity, and of 3.24, 3.76, 8.83 and 14.67 µM, respectively, for HBeAg with no cytotoxicity. (7'R,8'S,7″R,8″S)-erythro-Strebluslignanol G and magnolol showed significant anti-HBV activities to inhibit the replication of HBV DNA with the IC50 values of 9.02 and 8.67 µM, respectively.


Assuntos
Antivirais/farmacologia , Vírus da Hepatite B/efeitos dos fármacos , Lignanas/farmacologia , Moraceae/química , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Antivirais/química , Antivirais/isolamento & purificação , Relação Dose-Resposta a Droga , Células Hep G2 , Humanos , Lignanas/química , Lignanas/isolamento & purificação , Testes de Sensibilidade Microbiana , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Relação Estrutura-Atividade
15.
Nat Prod Res ; 37(3): 417-423, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34937443

RESUMO

A new lactone, asperlactone A (1), and four known lactone derivatives 2-5 were isolated from the mangrove endophytic fungus Aspergillus sp. GXNU-A9. Their structures were elucidated based on high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) datum, extensive nuclear magnetic resonance (NMR) spectroscopic analysis, and comparison with literature data. The structure of 1 was further confirmed by single-crystal X-ray diffraction analysis, and the absolute configuration of 1 was established. Compounds 1-5 were evaluated for their anti-inflammatory activities against nitric oxide (NO) production, and compounds 1-5 showed moderate inhibitory activities with IC50 values ranging from 15.87 to 30.48 µM.


Assuntos
Aspergillus , Lactonas , Aspergillus/química , Fungos , Cristalografia por Raios X , Espectroscopia de Ressonância Magnética , Estrutura Molecular
16.
Phytochemistry ; 208: 113606, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36736939

RESUMO

Twelve undescribed megastigmane glycosides, streilicifolosides A-L (1-12), together with 8 known analogues (13-21) were isolated from the leaves of Streblus ilicifolius (S.Vidal) Corner. Their plannar structures were elucidated using extensive NMR spectroscopic methods (1D and 2D-NMR spectroscopy), and HRESIMS spectroscopic data analyses. The absolute configurations of the undescribed compounds were determined by the glucose-induced shift-trend, calculated and experimental circular dichroism spectroscopy. All the compounds were tested for inhibitory effects on the production of NO in LPS-treated RAW264.7 cells, and streilicifoloside E and platanionoside D exhibited potent anti-inflammatory activity comparable to that of the positive control, with IC50 values of 26.33 and 21.84 µM, respectively. Furthermore, these two compounds markedly decreased the secretion of PGE2 and TNF-α and inhibited the expression of COX‒2, iNOS and NF-κB/p65 in LPS-induced RAW264.7 cells in a dose-dependent manner. In addition, the structure-activity relationships of the isolates were also discussed. The results suggest that streilicifoloside E and platanionoside D could be used as potential candidates for the development of new anti-inflammatory agents.


Assuntos
Glicosídeos , Moraceae , Animais , Camundongos , Glicosídeos/química , Norisoprenoides , Lipopolissacarídeos , Anti-Inflamatórios/farmacologia , Células RAW 264.7 , Moraceae/metabolismo , Óxido Nítrico , Estrutura Molecular
17.
Front Med (Lausanne) ; 10: 1150321, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37113608

RESUMO

Background and purpose: Early diagnosis is important for treatment and prognosis of obstructive sleep apnea (OSA)in children. Polysomnography (PSG) is the gold standard for the diagnosis of OSA. However, due to various reasons, such as inconvenient implementation, less equipped in primary medical institutions, etc., it is less used in children, especially in young children. This study aims to establish a new diagnostic method with imaging data of upper airway and clinical signs and symptoms. Methods: In this retrospective study, clinical and imaging data were collected from children ≤10 years old who underwent nasopharynx CT scan(low-dose protocol)from February 2019 to June 2020,including 25 children with OSA and 105 non-OSA. The information of the upper airway (A-line; N-line; nasal gap; upper airway volume; upper and lower diameter, left and right diameter and cross-sectional area of the narrowest part of the upper airway) were measured in transaxial, coronal, and sagittal images. The diagnosis of OSA and adenoid size were given according to the guidelines and consensus of imaging experts. The information of clinical signs, symptoms, and others were obtained from medical records. According to the weight of each index on OSA, the indexes with statistical significance were screened out, then were scored and summed up. ROC analysis was performed with the sum as the test variable and OSA as the status variable to evaluate the diagnostic efficacy on OSA. Results: The AUC of the summed scores (ANMAH score) of upper airway morphology and clinical index for the diagnosis of OSA was 0.984 (95% CI 0.964-1.000). When sum = 7 was used as the threshold (participants with sum>7 were considered to have OSA), the Youden's index reached its maximum at which point the sensitivity was 88.0%, the specificity was 98.1%, and the accuracy was 96.2%. Conclusion: The morphological data of the upper airway based on CT volume scan images combined with clinical indices have high diagnostic value for OSA in children; CT volume scanning plays a great guiding role in the selection of treatment scheme of OSA. It is a convenient, accurate and informative diagnostic method with a great help to improving prognosis. Highlights: - Early diagnosis of OSA in children is very important for the treatment.- However, the traditional diagnostic gold-standard PSG is difficult to implement.- This study aims to explore convenient and reliable diagnostic methods for children.- A new diagnostic model was established combining CT with signs and symptoms.- The diagnostic method in this study is highly effective, informative, and convenient.

18.
Mar Drugs ; 10(1): 234-241, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22363233

RESUMO

Four new bisabolane-type sesquiterpenoids, aspergiterpenoid A (1), (-)-sydonol (2), (-)-sydonic acid (3), and (-)-5-(hydroxymethyl)-2-(2',6',6'-trimethyltetrahydro-2H- pyran-2-yl)phenol (4) together with one known fungal metabolite (5) were isolated from the fermentation broth of a marine-derived fungus Aspergillus sp., which was isolated from the sponge Xestospongia testudinaria collected from the South China Sea. Four of them (1-4) are optically active compounds. Their structures and absolute configurations were elucidated by using NMR spectroscopic techniques and mass spectrometric analysis, and by comparing their optical rotations with those related known analogues. Compounds 1-5 showed selective antibacterial activity against eight bacterial strains with the MIC (minimum inhibiting concentrations) values between 1.25 and 20.0 µM. The cytotoxic, antifouling, and acetylcholinesterase inhibitory activities of these compounds were also examined.


Assuntos
Antibacterianos/isolamento & purificação , Aspergillus/metabolismo , Poríferos/microbiologia , Sesquiterpenos/isolamento & purificação , Animais , Antibacterianos/farmacologia , Aspergillus/química , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Sesquiterpenos/química , Sesquiterpenos/farmacologia
19.
Nat Prod Res ; 36(7): 1878-1882, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32851874

RESUMO

A new tetracyclic depsidone derivative, guanxidone A (1), together with three known metabolites 2-4, was isolated from the mangrove endophytic fungus Aspergillus sp. GXNU-A9. The structure of compound 1 was established by HR-ESI-MS, 1 D and 2 D NMR data, as well as by comparison with literature data. Compounds 1-4 were evaluated for their anti-inflammatory effects on the production of the nitric oxide (NO), and compound 1 significantly reduced the production of NO in lipopolysaccharide (LPS)-stimulated cells with IC50 value of 8.22 µM.


Assuntos
Aspergillus , Fungos , Aspergillus/química , Depsídeos , Fungos/metabolismo , Lactonas , Lipopolissacarídeos , Estrutura Molecular , Óxido Nítrico/metabolismo
20.
Nat Prod Res ; 36(7): 1857-1863, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32962429

RESUMO

A new sesquiterpene, gxsespene A (1), and four known sesquiterpene derivatives 2-5 were isolated from the mangrove endophytic fungus Aspergillus sp. GXNU-MA1. Their structures were elucidated based on high-resolution electrospray ionization mass spectroscopy (HR-ESI-MS) datum, extensive nuclear magnetic resonance (NMR) spectroscopic analysis, and comparison with literature data. The structure of 1 was further confirmed by single-crystal X-ray diffraction analysis, and the absolute configuration of 1 was established. Compounds 1-5 were evaluated for their anti-inflammatory activities against the nitric oxide (NO) production, and compounds 1-5 showed moderate inhibitory activities with IC50 values ranging from 16.15 to 27.08 µM.


Assuntos
Aspergillus , Sesquiterpenos , Aspergillus/química , Cristalografia por Raios X , Fungos , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Sesquiterpenos/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA