Effects of plasticizer diisobutyl adipate on the Japanese medaka (Oryzias latipes) endocrine system.

Plasticizer pollution of the water environment is one of the world's most serious environmental issues. Phthalate plasticizers can disrupt endocrine function in vertebrates. Therefore, this study analyzed thyroid-related, reproduction-related, and estrogen-responsive genes in Japanese medaka (Oryzias latipes) to determine whether non-phthalate diisobutyl adipate (DIBA) plasticizer could affect endocrine hormone activity or not. Developmental toxicity during fish embryogenesis was also evaluated. At a concentration of 11.57 mg/l, embryonic exposure to DIBA increased the mortality rate. Although abnormal development, including body curvature, edema, and lack of swim bladder inflation, was observed at 3.54 and 11.57 mg/l DIBA, growth inhibition and reduced swimming performance were also observed. In addition, DIBA exposure increased the levels of thyroid-stimulating hormone beta-subunit (tshß) and deiodinase 1 (dio1) but decreased the levels of thyroid hormone receptor alpha (trα) and beta (trß). These results suggest that DIBA has thyroid hormone-disrupting activities in fish. However, kisspeptin (kiss1 and kiss2), gonadotropin-releasing hormone (gnrh1), follicle-stimulating hormone beta (fshß), luteinizing hormone beta (lhß), choriogenin H (chgH), and vitellogenin (vtg1) expression did not change dose-dependently in response to DIBA exposure, whereas gnrh2 and vtg2 expression was elevated. These results indicate that DIBA has low estrogenic activity and does not disrupt the endocrine reproduction system in fish. Overall, this is the first report indicating that non-phthalate DIBA plasticizer is embryotoxic and disrupt thyroid hormone activity in fish.

Immunomodulatory Effect and an Intervention of TNF Signalling Leading to Apoptotic and Cell Cycle Arrest on ORL-204 Oral Cancer Cells by Tiger Milk Mushroom, Lignosus rhinocerus.

Research background: Tiger milk mushroom (Lignosus rhinocerus) is a medicinal mushroom that is geographically distributed in the region of South China, Thailand, Malaysia, Indonesia, Philippines and Papua New Guinea. Consumption of its sclerotium has been reported to treat various ailments. However, its anticancer potential towards oral cancer cell lines is yet to be determined considering the traditional method of its consumption by biting/chewing of the sclerotium. Experimental approach: Mushroom sclerotial powder of cultivar TM02® was extracted and fractionated in a chromatographic column prior to cytotoxicity testing against a panel of human oral cancer cell lines. The capability of the identified bioactive fraction in regulating several molecules associated with its tumour necrosis factor (TNF) pathway was investigated. Results and conclusions: 2,5-Diphenyl-2H-tetrazolium bromide (MTT) proliferation assay indicated that cell lines ORL-48 (derived from gingiva), ORL-188 (derived from the tongue) and ORL-204 (derived from buccal mucosa) were inhibited by cold water extract of L. rhinocerus sclerotia and its high-molecular-mass fraction (HMM) in varying degrees with ORL-204 being most affected. Hence, the treatment of ORL-204 with HMM mushroom extract was further investigated. HMM mushroom extract induced apoptosis and G0/G1 phase cell cycle arrest through caspase-3/7 cleavage. Activities of MIP2 and COX-2 were downregulated by 0.2- and 4.6-fold respectively in the HMM mushroom extract-treated ORL-204 cells. Novelty and scientific contribution: Using ORL-204, we showed that HMM mushroom extract may act via the TNF pathway at various network sites as a potential dietary compound for cancer prevention and natural adjunct therapeutic to conventional cancer treatment.

Antioxidant and Cytotoxic Effects and Identification of Ophiocordyceps sinensis Bioactive Proteins Using Shotgun Proteomic Analysis.

RESEARCH BACKGROUND: Ophiocordyceps sinensis, a highly valued medicinal fungus, is close to extinction due to overexploitation. Successful cultivation of O. sinensis fruiting body (OCS02®) shows that the cultivar has a promising nutritional value and numerous bioactive compounds. Antioxidant and antiproliferative properties and biologically active proteins of the OCS02® are investigated for possible development into nutraceuticals. EXPERIMENTAL APPROACH: The chemical composition of the OCS02® cold water extract was determined, and the antioxidant activities were examined using ferric reducing, DPPH• and O2 •- scavenging assays. Tetrazolium dye (MTT) cytotoxic assay was performed to assess the antiproliferative activity of the extract. Bioactive proteins in the active fraction of the extract were identified using liquid chromatography (LC) and tandem-mass spectrometry (MS/MS). RESULTS AND CONCLUSIONS: The OCS02® extract exhibited strong O2 •- scavenging (expressed as Trolox equivalents (18.4±1.1) mol/g) and potent cytotoxic activities against adenocarcinomic human alveolar basal epithelial (A549) cells (IC50=(58.2±6.8) µg/mL). High molecular mass polysaccharides, proteins and protein-polysaccharide complexes could have contributed to the antioxidant and cytotoxic selectivity of the OCS02®. LC-MS/MS analysis identified several potential cytotoxic proteases and an oxalate decarboxylase protein which may exhibit protection effects on kidneys. NOVELTY AND SCIENTIFIC CONTRIBUTIONS: The findings demonstrate the potential of OCS02® to be developed into functional food due to its promising superoxide anion radical scavenging capacity, cytotoxic effect and presence of biopharmaceutically active proteins.

Mucoactive agents for acute respiratory failure in the critically ill: a systematic review and meta-analysis.

PURPOSE: Acute respiratory failure (ARF) is a common cause of admission to intensive care units (ICUs). Mucoactive agents are medications that promote mucus clearance and are frequently administered in patients with ARF, despite a lack of evidence to underpin clinical decision making. The aim of this systematic review was to determine if the use of mucoactive agents in patients with ARF improves clinical outcomes. METHODS: We searched electronic and grey literature (January 2020). Two reviewers independently screened, selected, extracted data and quality assessed studies. We included trials of adults receiving ventilatory support for ARF and involving at least one mucoactive agent compared with placebo or standard care. Outcomes included duration of mechanical ventilation. Meta-analysis was undertaken using random-effects modelling and certainty of the evidence was assessed using Grades of Recommendation, Assessment, Development and Evaluation. RESULTS: Thirteen randomised controlled trials were included (1712 patients), investigating four different mucoactive agents. Mucoactive agents showed no effect on duration of mechanical ventilation (seven trials, mean difference (MD) -1.34, 95% CI -2.97 to 0.29, I2=82%, very low certainty) or mortality, hospital stay and ventilator-free days. There was an effect on reducing ICU length of stay in the mucoactive agent groups (10 trials, MD -3.22, 95% CI -5.49 to -0.96, I2=89%, very low certainty). CONCLUSION: Our findings do not support the use of mucoactive agents in critically ill patients with ARF. The existing evidence is of low quality. High-quality randomised controlled trials are needed to determine the role of specific mucoactive agents in critically ill patients with ARF. PROSPERO REGISTRATION NUMBER: CRD42018095408.

SIAN: software for structural identifiability analysis of ODE models.

SUMMARY: Biological processes are often modeled by ordinary differential equations with unknown parameters. The unknown parameters are usually estimated from experimental data. In some cases, due to the structure of the model, this estimation problem does not have a unique solution even in the case of continuous noise-free data. It is therefore desirable to check the uniqueness a priori before carrying out actual experiments. We present a new software SIAN (Structural Identifiability ANalyser) that does this. Our software can tackle problems that could not be tackled by previously developed packages. AVAILABILITY AND IMPLEMENTATION: SIAN is open-source software written in Maple and is available at https://github.com/pogudingleb/SIAN. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Prevention is better than cure: Persian Gulf biodiversity vulnerability to the impacts of desalination plants.

Due to extremely high rates of evaporation and low precipitation in the Persian Gulf, discharges from desalination plants (DPs) can lead to ecological stresses by increasing water temperatures, salinities, and heavy metal concentrations, as well as decreasing dissolved oxygen levels. We discuss the potential ecological impacts of DPs on marine organisms and propose mitigating measures to reduce the problems induced by DPs discharges. The daily capacity of DPs in the Persian Gulf exceeds 11 million m3 per day, which is approximately half of global daily freshwater production; multistage flash distillation (MSF) is the dominant desalination process. Results from field and laboratory studies indicate that there are potentially serious and chronic threats to marine communities following exposure to DP discharges, especially within the zoobenthos, echinodermata, seagrasses, and coral reefs. DP discharges can lead to decreases in sensitive species, plankton abundance, hard substrate epifauna, and growth rates of seagrasses. However, the broad applicability of any one of these impacts is currently hard to scale because of the limited number of studies that have been conducted to assess the ecological impacts of DP discharge on Persian Gulf organisms. Even so, available data suggest that appropriately sited, designed, and operated DPs combined with current developments in impingement and entrainment reduction technology can mitigate many of the negative environmental impacts of DPs.

A comparison of biomarker responses in juvenile diploid and triploid African catfish, Clarias gariepinus, exposed to the pesticide butachlor.

Influence of waterborne butachlor (BUC), a commonly used pesticide, on morphometric, biochemical, and molecular biomarkers was evaluated in juvenile, full sibling, diploid and triploid African catfish (Clarias gariepinus). Fish were exposed for 21 days to one of three concentrations of BUC [mean measured µg/L: 22, 44 or 60]. Unexposed (control) triploids were heavier and longer and had higher visceral-somatic index (VSI) than diploids. Also, they had lighter liver weight (HSI) and showed lower transcript levels of brain gonadotropin-releasing hormone (GnRH), aromatase (cyp191b) and fushi tarazu-factor (ftz-f1), and plasma testosterone levels than diploids. Butachlor treatments had no effects, in either diploid or triploid fish, on VSI, HSI, weight or length changes, condition factor (CF), levels of plasma testosterone, 17-ß estradiol (E2), cortisol, cholesterol, or mRNA levels of brain tryptophan hydroxylase (tph2), forkhead box L2 (foxl2), and 11 ß-hydroxysteroid dehydrogenase type 2 (11ß-hsd2). Expressions of cyp191b and ftz-f1 in triploids were upregulated by the two highest concentrations of BUC. In diploid fish, however, exposures to all BUC concentrations decreased GnRH transcription and the medium BUC concentration decreased ftz-f1 transcription. Substantial differences between ploidies in basal biomarker responses are consistent with the reported impaired reproductive axis in triploid C. gariepinus. Furthermore, the present study showed the low impact of short term exposure to BUC on reproductive axis in C. gariepinus.

Effects of metal-contaminated soils on the accumulation of heavy metals in gotu kola (Centella asiatica) and the potential health risks: a study in Peninsular Malaysia.

Centella asiatica is a commonly used medicinal plant in Malaysia. As heavy metal accumulation in medicinal plants which are highly consumed by human is a serious issue, thus the assessment of heavy metals in C. asiatica is important for the safety of consumers. In this study, the heavy metal accumulation in C. asiatica and the potential health risks were investigated. Samples of C. asiatica and surface soils were collected from nine different sites around Peninsular Malaysia. The concentration of six heavy metals namely Cd, Cu, Ni, Fe, Pb and Zn were determined by air-acetylene flame atomic absorption spectrophotometer (AAS). The degree of anthropogenic influence was assessed by calculating the enrichment factor (EF) and index of geoaccumulation (Igeo). The heavy metal uptake into the plant was estimated through the calculation of translocation factor (TF), bioconcentration factor (BCF) and correlation study. Estimated daily intakes (EDI) and target hazard quotients (THQ) were used to determine the potential health risk of consuming C. asiatica. The results showed that the overall surface soil was polluted by Cd, Cu and Pb, while the uptake of Zn and Ni by the plants was high. The value of EDI and THQ showed that the potential of Pb toxicity in C. asiatica was high as well. As heavy metal accumulation was confirmed in C. asiatica, daily consumption of the plant derived from polluted sites in Malaysia was not recommended.

Potential human health risk assessment of heavy metals via the consumption of tilapia Oreochromis mossambicus collected from contaminated and uncontaminated ponds.

Fish tilapia Oreochromis mossambicus were collected from a contaminated Seri Serdang (SS) pond potentially receiving domestic effluents and an uncontaminated pond from Universiti Putra Malaysia (UPM). The fish were dissected into four parts namely gills, muscles, intestines, and liver. All the fish parts were pooled and analyzed for the concentrations of Cd, Cu, Fe, Ni, Pb, and Zn. Generally, the concentrations of all metals were low in the edible muscle in comparison to the other parts of the fish. It was found that the levels of all the heavy metals in the different parts of fish collected from the SS were significantly (P<0.05) higher than those from UPM, indicating greater metal bioavailabilities in the SS pond. The sediment data also showed a similar pattern with significantly (P<0.05) higher metal concentrations in SS than in UPM, indicating higher metal contamination in SS. Potential health risk assessments based on provisional tolerable weekly intake (PTWI) and the amount of fish required to reach the PTWI values, estimated daily intake (EDI), and target hazard quotient (THQ) indicated that health risks associated with heavy metal exposure via consumption of the fish's muscles were insignificant to human. Therefore, the consumption of the edible muscles of tilapia from both ponds should pose no toxicological risk of heavy metals since their levels are also below the recommended safety guidelines. While it is advisable to discard the livers, gills, and intestines of the two tilapia fish populations before consumption, there were no potential human health risks of heavy metals to the consumers on the fish muscle part.

A Conceptual Model Relationship between Industry 4.0-Food-Agriculture Nexus and Agroecosystem: A Literature Review and Knowledge Gaps.

With the expected colonization of human daily life by artificial intelligence, including in industry productivity, the deployment of Industry 4.0 (I4) in the food agriculture industry (FAI) is expected to revolutionize and galvanize food production to increase the efficiency of the industry's production and to match, in tandem, a country's gross domestic productivity. Based on a literature review, there have been almost no direct relationships between the I4-Food-Agriculture (I4FA) Nexus and the agroecosystem. This study aimed to evaluate the state-of-the-art relationships between the I4FA Nexus and the agroecosystem and to discuss the challenges in the sustainable FAI that can be assisted by the I4 technologies. This objective was fulfilled by (a) reviewing all the relevant publications and (b) drawing a conceptual relationship between the I4FA Nexus and the agroecosystem, in which the I4FA Nexus is categorized into socio-economic and environmental (SEE) perspectives. Four points are highlighted in the present review. First, I4 technology is projected to grow in the agricultural and food sectors today and in the future. Second, food agriculture output may benefit from I4 by considering the SEE benefits. Third, implementing I4 is a challenging journey for the sustainable FAI, especially for the small to medium enterprises (SMEs). Fourth, environmental, social, and governance (ESG) principles can help to manage I4's implementation in agriculture and food. The advantages of I4 deployment include (a) social benefits like increased occupational safety, workers' health, and food quality, security, and safety; (b) economic benefits, like using sensors to reduce agricultural food production costs, and the food supply chain; and (c) environmental benefits like reducing chemical leaching and fertilizer use. However, more studies are needed to address social adaptability, trust, privacy, and economic income uncertainty, especially in SMEs or in businesses or nations with lower resources; this will require time for adaptation to make the transition away from human ecology. For agriculture to be ESG-sustainable, the deployment of I4FA could be an answer with the support of an open-minded dialogue platform with ESG-minded leaders to complement sustainable agroecosystems on a global scale.

Phenytoin causes behavioral abnormalities and suppresses kisspeptin expression, reducing reproductive performance in Japanese medaka.

Phenytoin, an antiepileptic drug, induces neurotoxicity and abnormal embryonic development and reduces spontaneous locomotor activity in fish. However, its effects on other endpoints remain unclear. Therefore, we investigated the effects of phenytoin on the swimming behavior and reproductive ability of Japanese medaka. Abnormalities in swimming behavior, such as imbalance, rotation, rollover, and vertical swimming, were observed. However, when phenytoin exposure was discontinued, the behavioral abnormality rates decreased. Phenytoin exposure also significantly reduced reproductive ability. By investigating reproduction-related gene expression of gnrh1, gnrh2, fshb, and lhb remained unchanged in males and females. In contrast, kiss1 expression was significantly suppressed due to phenytoin exposure in males and females. kiss2 expression was also significantly suppressed in females but not in males. We filmed videos to examine phenytoin exposure effects on sexual behavior. Females showed no interest in the male's courtship. As the kisspeptin 1 system controls sexual behavior in Japanese medaka, phenytoin exposure may have decreased kiss1 expression, which decreased female reproductive motivation; hence, they did not spawn eggs. This is the first study to show that phenytoin exposure induces behavioral abnormalities, and suppresses kiss1 expression and reproductive performance in Japanese medaka.

Atypical Hemorrhages Secondary to Dengue Infection and Cardiopulmonary Resuscitation: Subcutaneous Thrombin Injection as a Salvage Technique for Refractory Femoral Artery Hemorrhage.

Dengue fever is a viral infection transmitted by the bites of female Aedes mosquitoes. Repeat infections with different viral serotypes are possible, with an increased risk of severe dengue. Dengue hemorrhagic fever is one of the most severe presentations of dengue, with thrombocytopenia, increased capillary permeability with resultant rash, and an increased risk of spontaneous bleeding. The management of severe dengue is done through supportive care and symptomatic management only, as there are no specific treatments available. We describe a case of severe dengue hemorrhagic fever presenting with atypical hemorrhage from both the psoas muscle and the femoral arterial puncture sites. These were successfully treated with large calibrated Gelfoam particle embolization for the psoas hemorrhage and regional thrombin injection for the femoral arterial puncture sites.

Effect of diisobutyl adipate on the expression of biomarker genes that respond to endocrine disruption and on gonadal sexual differentiation in Japanese medaka (Oryzias latipes).

Phthalate and non-phthalate plasticizers are used in polymer materials, such as plastic and rubber. It has recently been found that diisobutyl adipate (DIBA), which is considered an environmentally safe non-phthalate plasticizer, potentially acts as a thyroid disruptor in fish. Here, we investigated the sexual hormone effects of DIBA based on the expression levels of genes that respond to endocrine disruption and sexual hormone activity in the livers and gonads, and on gonadal sexual differentiation in Japanese medaka. Compared with the control group, the mRNA expression of chgH, vtg1, vtg2, and esr1 was significantly suppressed in the livers of DIBA exposed XX individuals. Furthermore, the mRNA expression of gsdf was significantly upregulated and downregulated in the gonads of XX and XY individuals, respectively. The mRNA expressions of esr1 and esr2b were significantly suppressed by DIBA exposure in the gonads of both XX and XY individuals. These observations suggest that DIBA has potential androgenic activity in Japanese medaka. However, normal testes and ovaries were observed in respective XY and XX medaka after DIBA exposure; therefore, these results suggest that DIBA may have weak androgenic activity.

Hyperparasitaemic human Plasmodium knowlesi infection with atypical morphology in peninsular Malaysia.

Plasmodium knowlesi is a potentially life-threatening zoonotic malaria parasite due to its relatively short erythrocytic cycle. Microscopic identification of P. knowlesi is difficult, with "compacted parasite cytoplasm" being one of the important identifying keys. This report is about a case of hyperparasitaemic human P. knowlesi infection (27% parasitaemia) with atypical amoeboid morphology. A peninsular Malaysian was admitted to the hospital with malaria. He suffered anaemia and acute kidney function impairment. Microscopic examination, assisted by nested PCR and sequencing confirmed as P. knowlesi infection. With anti-malarial treatment and several medical interventions, patient survived and recovered. One-month medical follow-up was performed after recovery and no recrudescence was noted. This case report highlights the extreme hyperparasitaemic setting, the atypical morphology of P. knowlesi in the patient's erythrocytes, as well as the medical interventions involved in this successfully treated case.

Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies.

Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird's DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species.

Comment on Peycheva et al. Trace Elements and Omega-3 Fatty Acids of Wild and Farmed Mussels (Mytilus galloprovincialis) Consumed in Bulgaria: Human Health Risks. Int. J. Environ. Res. Public Health 2021, 18, 10023.

First of all, the interesting paper by Peycheva et al. [...].

Byssus of Green-Lipped Mussel Perna viridis as a Biomonitoring Biopolymer for Zinc Pollution in Coastal Waters.

The present study aimed to confirm the use of the byssus (BYS) of the green-lipped mussel Perna viridis as a biomonitoring biopolymer for zinc (Zn) by comparing it to copper (Cu) and cadmium (Cd) pollution in coastal waters under experimental field conditions, based on the transplantation of caged mussels between polluted and unpolluted sites in the Straits of Johore (SOJ). Four important evidential points were found in the present study. First, the 34 field-collected populations with BYS/total soft tissue (TST) ratios > 1 indicated that the BYS was a more sensitive, concentrative, and accumulative biopolymer for the three metals than TST. Significant (p < 0.05) and positive correlations between BYS and TST in terms of the levels of the three metals were observed. Second, the data obtained in the present study were well-supported by the interspecific comparison, which indicated that the BYS of P. viridis was a significantly better biomonitoring biopolymer for the identification of coastal areas exposed to Zn, Cd, and Cu pollution and played the role of an excretion route of metal wastes. Third, the higher positive correlation coefficients for the metals between the BYS sedimentary geochemical fractions than the TST sedimentary geochemical fractions indicated that the BYS was more reflective of metal bioavailability and contamination in coastal waters. Fourth, and most importantly, the field-based cage transplantation study clearly indicated the accumulation and elimination of the three metals by the BYS in both polluted and unpolluted sites in the Straits of Johore. In sum, the BYS of P. viridis was confirmed as a better biopolymer than TST for Zn, as well as Cd and Cu, bioavailability and contamination in tropical coastal waters.

Biomonitoring-Health Risk Nexus of Potentially Toxic Metals on Cerithidea obtusa: A Biomonitoring Study from Peninsular Malaysia.

The present study aimed to assess the human health risks of six potentially toxic metals (PTMs) (Cd, Cu, Fe, Ni, Pb and Zn) in 21 populations of popular mangrove snails, Cerithidea obtusa, collected from Malaysia. In general, the concentrations (mg/kg wet weight) of Cd (0.03-2.32), Cu (11.4-35.2), Fe (40.9-759), Ni (0.40-6.14), Pb (0.90-13.4) and Zn (3.11-129) found in the snails in all populations were lower than the prescribed maximum permissible limits (MPL)s for Cd, Cu, Ni, Pb and Zn. However, in the investigated snail populations, Cd (14%), Pb (62%), Cu (19%), and Zn (10%) were found in exceedance of the MPL of the respective metal. The target hazard quotient (THQ) values in all populations for Cu, Ni, Fe and Zn were all found to be below 1.00. However, for the THQ values of Cd and Pb, two populations exceeded 1.00, while others were below the threshold level. The estimated weekly intake (EWI) of all six metals for all populations was only 0.03-4.65% of the provisional tolerable weekly intake. This conclusively indicates that, based on the EWI, there are no health risks of the six PTMs in the consumption of snails from Malaysia since the assessments are dependent on the consumers' body weight and consumption rate. Nonetheless, the present results indicate that the amounts of snails consumed should be limited to minimize the potential health risks of PTMs to consumers. The relatively low and weak but positive correlations of Cu, Ni, Pb and Zn between C. obtusa and their habitat sediments indicate that C. obtusa can be a potential biomonitor for Cu, Ni, Pb and Zn. This is important for effective mangrove management from the perspective of the sustainable resources from the intertidal mangrove environment. Hence, the biomonitoring-health risk nexus of PTMs in mangrove snails is proposed in the present study.

Effective Microorganisms as Halal-Based Sources for Biofertilizer Production and Some Socio-Economic Insights: A Review.

This paper aims to review the literature on 'Effective Microorganism (EM)' and 'Fertilizer' from the Scopus database and to discuss EMs using Halal-based sources for biofertilizer production from socio economic insights. Based on EM and fertilizer publications on the Scopus database, all the 17 papers reviewed provided no detailed information on the Halal-status of the biofertilizers inoculated with EM. The impacts of Halal-certified biofertilizers will trigger the Halal certification in food products by (a) catering for the increasing Halal food demand due to expectedly Muslim population expansion, (b) contributing to the sustainable buying behaviour of Halal products' consumers in the future, (c) catering for the increasing number of Muslim travellers around the world, (d) becoming a positive driver for higher production of more Halal foods that can enhance food safety, human health and well-being, and (e) creating a cost-effective and increasing food marketability. The later three points (c, d and e) play a very important role in a country's societal well-being and economic growth and development. Although Halal-status is not a must for the world's food marketing, Halal-certified biofertilizer for the Halal-status of food carries the greatest potential to enter the ever-expanding Muslim markets. Finally, it is postulated that the successful usage of EM using Halal-based sources for biofertilizer production will result in two major outcomes from the points of United Nations' Sustainable Development Goals # 9 (Industry, Innovation and Infrastructure) and # 12 (Responsible Consumption and Production). Hence, the presented review provides a starting point for future research considering sustainability and innovation as priorities.

Lower Health Risks of Potentially Toxic Metals after Transplantation of Aquacultural Farmed Mussels from a Polluted Site to Unpolluted Sites: A Biomonitoring Study in the Straits of Johore.

The present field-based study aimed to determine the levels of six potentially toxic metals (PTM)s (Cd, Cu, Fe, Ni, Pb, and Zn determined using a flame atomic-absorption spectrophotometer) using transplanted green-lipped mussel Perna viridis from a polluted site at Kampung Pasir Puteh (KPP) to unpolluted sites at Kampung Sungai Melayu (KSM) and Sungai Belungkor (SB) in the Johore Straits (SOJ), and to estimate the human health risks of the PTMs after the depuration periods. Interestingly, after 10 weeks of depuration in the two unpolluted sites, there were 55.6-88.4% and 51.3-91.7% reductions of the six PTMs after transplantation from KPP to SB and KSM, respectively. Lower risks of health assessments were recorded and judged on the present findings of significantly (p < 0.05) lower levels of safety guidelines, significantly (p < 0.05) lower values of target hazard quotient, and significantly (p < 0.05) lower values of estimated weekly intake, of all the six PTMs after 10 weeks of depuration of the transplanted polluted mussels to the two unpolluted sites in the SOJ. Thus, further reducing the noncarcinogenic risks of the PTMs to the consumers. From an aquacultural point of view, this depuration technique can be recommended to reduce the health risks of PTMs to mussel consumers.