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Identification and characterization of proteins involved in salt tolerance are imperative for revealing its genetic mechanisms. In this study, ionic and proteomic responses of a Tibetan wild barley XZ16 and a well-known salt-tolerant barley cv. CM72 were analyzed using inductively coupled plasma-optical emission spectrometer, 2DE, and MALDI-TOF/TOF MS techniques to determine salt-induced differences in element and protein profiles between the two genotypes. In total, 41 differentially expressed proteins were identified in roots and leaves, and they were associated with ion homeostasis, cell redox homeostasis, metabolic process, and photosynthesis. Under salinity stress, calmodulin, Na/K transporters, and H(+) -ATPases were involved in establishment of ion homeostasis for barley plants. Moreover, ribulose-1,5-bisphosphate carboxylase/oxygenase activase and oxygen-evolving enhancer proteins were significantly upregulated under salinity stress, indicating the great impact of salinity on photosynthesis. In comparison with CM72, XZ16 had greater relative dry weight and lower Na accumulation in the shoots under salinity stress. A higher expression of HvNHX1 in the roots, and some specific proteins responsible for ion homeostasis and cell redox homeostasis, was also found in XZ16 exposed to salt stress. The current results showed that Tibetan wild barley XZ16 and cultivated barley cultivar CM72 differ in the mechanism of salt tolerance.
Assuntos
Hordeum/fisiologia , Proteínas de Plantas/metabolismo , Tolerância ao Sal , Eletroforese em Gel Bidimensional , Regulação da Expressão Gênica de Plantas , Genótipo , Homeostase , Hordeum/genética , Íons/metabolismo , Proteínas de Plantas/análise , Proteínas de Plantas/genética , Proteômica , Salinidade , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Estresse FisiológicoRESUMO
In the field of power ultrasonic vibration processing, the thin liquid layer nestled between the tool head and the material serves as a hotbed for cavitation shock wave emissions that significantly affect the material's surface. The precise manipulation of these emissions presents a formidable challenge, stemming from a historical deficit in the quantitative analysis of both the ultrasonic enhancement effect and the shock wave intensity within this niche environment. Our study addresses this gap by innovatively modifying the Gilmore-Akulichev equation, laying the groundwork for a sophisticated bubble dynamics model and a pioneering shock wave propagation model tailored to the thin liquid layer domain. Firstly, our study investigated the ultrasound enhancement effect under various parameters of thin liquid layers, revealing an amplification of ultrasound pressure in the thin liquid layer area by up to 7.47 times. The mathematical model was solved using the sixth-order Runge-Kutta method to examine shock wave velocity and pressure under different conditions. our study identified that geometric parameters of the tool head, thin liquid layer thickness, ultrasonic frequency, and initial bubble radius all significantly influenced shock wave emission. At an ultrasonic frequency of 60 kHz, the shock wave pressure at the measurement point exhibited a brief decrease from 182.6 to 179.5 MPa during an increase. Furthermore, rapid attenuation of the shock wave was found within the range of R0-3R0 from the bubble wall. This research model aims to enhance power ultrasonic vibration processing technology, and provide theoretical support for applications in related fields.
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In industrial production and scientific research, ultrasonic cavitation technology, with its outstanding physical and chemical processing capabilities, has been widely applied in fields such as material surface modification, chemical synthesis, and biotechnology, becoming a focal point of research and application. This article delves into the effects of different ultrasonic frequencies on cavitation outcomes through the combined use of numerical simulation, fluorescence analysis, and high-speed photography, specifically analyzing the quantitative improvement in the mechanical properties of TC17 titanium alloy under ultrasonic cavitation at frequencies of 20 kHz, 30 kHz, and 40 kHz. The study found that at an ultrasonic frequency of 20 kHz, the maximum expansion radius of cavitation bubbles can reach 51.4 µm, 8.6 times their initial radius. Correspondingly, fluorescence intensity and peak area also increased to 402.8 and 28104, significantly above the baseline level. Moreover, after modification by ultrasonic cavitation, the original machining marks on the surface of TC17 titanium alloy became fainter, with the emergence of new, uniformly distributed microfeatures. The microhardness of the material increased from 373.7 Hv to 383.84 Hv, 396.62 Hv, and 414.06 Hv, with a maximum improvement of 10.8 %. At the same time, surface height difference and roughness significantly decreased (to 3.168 µm and 0.61 µm respectively), with reductions reaching 45.1 % and 42.4 %, indicating a significant improvement in material surface quality. Notably, there is a negative correlation between the improvement of mechanical properties and ultrasonic frequency, suggesting that the improvement effects decrease as ultrasonic frequency increases. This research not only reveals the quantitative relationship between ultrasonic cavitation frequency and material surface modification effects but also provides a solid scientific basis and practical guidance for the application of ultrasonic cavitation technology in surface engineering, signifying the technology's potential for broad application in the future.
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Ceramic matrix composites have the advantages of low density, high specific strength, high specific die, high-temperature resistance, wear resistance, chemical corrosion resistance, etc., which are widely used in aerospace, energy, transportation, and other fields. CMCs have become an important choice for engine components and other high-temperature component manufacturing. However, ceramic matrix composite is a kind of multi-phase structure, anisotropy, high hardness material, due to the brittleness of the ceramic matrix, the weak bonding force between fiber and matrix, and the anisotropy of composite material. Burr, delamination, tearing, chips, and other surface damage tend to generate in the machining, resulting in surface quality and strength decline. This paper reviewed the latest abrasive machining technology for SiC ceramic composites. The characteristics and research directions of the main abrasive machining technology, including grinding, laser-assisted grinding, ultrasonic-assisted grinding, and abrasive waterjet machining, are introduced first. Then, the commonly used numerical simulation research for modeling and simulating the machining of ceramic matrix composites is briefly summarized. Finally, the processing difficulties and research hotspots of ceramic matrix composites are summarized.
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High-speed micro-jet produced by cavitation collapse near the wall is the main mechanism of material damage, and cavitation pit is the most typical damage feature. The reason why high-pressure and high-speed micro-jet can only cause nano- and microscale cavitation pit is that the micro-jet is a short-term impact load of nano- and microscale, and the material shows size effect during the formation of pits. To further explore the cavitation damage characteristics and deformation mechanism of materials, the theoretical framework of indentation test and J-C constitutive model were adopted, and the size effect of materials during the process of cavitation pit formation was mainly considered, and the prediction models of cavitation impact load, impact pressure and velocity of micro-jet were established. The results showed that the equivalent stress and strain of cavitation pit and the impact pressure and velocity of micro-jet are only related to the diameter-to-depth ratio of pit without size effect, and also to the diameter of pit with size effect. Larger diameter and deeper depth of the pit infers greater cavitation impact load, and the influence of the pit diameter is more obvious. When considering the size effect, there is an additional size effect coefficient: 1+54hpα2µ2bdp2σJC2. In the selected size range of pit, the cavitation impact load, impact pressure and velocity of micro-jet predicted with size effect increase by 0.9408%-322.5% compared with those without size effect. The maximum increase ratio appears at the minimum of diameter-to-depth ratio of pit (dp = 2 µm and dh = 2 µm), that is, the smaller the pit diameter is and the greater the depth is, the greater the increase ratio is. Ten typical cavitation pits were selected for inversion analysis. The impact pressure and velocity of micro-jet with and without size effect are 473-1131 MPa and 355-848 m/s, and 427-604 MPa and 320-453 m/s, respectively. The predicted values increase by about 11%-88% when considering the size effect, and the micro-jet velocity predicted is closer to that observed by high-speed cameras, which confirms the necessity and rationality of size effect in the inversion analysis of cavitation pits.
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For the analysis of ultrasonic cavitation erosion on the surface of materials, the ultrasonic cavitation erosion experiments for AlCu4Mg1 and Ti6Al4V were carried out, and the changes of surface topography, surface roughness, and Vickers hardness were explored. Cavitation pits gradually expand and deepen with the increase of experiment time, and Ti6Al4V is more difficult to erode by cavitation than AlCu4Mg1. After experiments, the cavitation damage characteristics such as the single pit, the rainbow ring area, the fisheye pit, and some small pits were observed, which can be considered to be induced by a single micro-jet impact, ablation effect caused by the high temperature, micro-jet impingement with a sharp angle, and multibeam micro-jets coupling impact or negative pressure in the local area produced by micro-jet impact, respectively. The surface roughness and Vickers hardness of the material increase slowly after rapid growth at different points in time as the experiment time increases. With the increase of the ultrasonic amplitude, both of them first increase and then decrease after the ultrasonic amplitude is greater than 10.8 µm. The increases in surface roughness and Vickers hardness tend to decrease as the viscosity coefficient increases. Ultrasonic cavitation can cause submicron surface roughness and increase surface hardness by 20.36%, so it can be used as a surface treatment method.
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Ultrasonic cavitation is a physical dynamic phenomenon of bubbles inflation, compression, and collapse in liquid. A dual-frequency ultrasonic cavitation dynamics model is established in this paper to investigate dynamic evolution of bubble under single and dual frequency ultrasonic modes. The variation of bubble radius, pressure, energy, temperature, and number of water vapor molecules inside the bubble in single and dual frequency ultrasonic modes are analyzed, respectively. The results show the oscillation of cavitation bubbles is more unstable and easier to collapse in dual-frequency ultrasound field than those in single-frequency ultrasound field. With the increase of the ultrasonic frequency, cavitation effect is weakened due to the shortage of oscillation period. Under the same ultrasonic power, the maximums of bubble radius, pressure, and water vapor molecules number inside the bubble in the dual-frequency mode are larger than those in the single-frequency mode. Under the ultrasonic excited by 50â¯kHzâ¯+â¯70â¯kHz, the maximum bubble radius and pressure can reach 36.061⯵m and 2285.9â¯MPa, respectively, which are much larger than 18.183⯵m, 730.61â¯MPa at 50â¯kHz and 14.576⯵m, 332.25â¯MPa at 70â¯kHz. The calculation results of three different frequency combinations (30â¯kHzâ¯+â¯50â¯kHz, 40â¯kHzâ¯+â¯60â¯kHz and 50â¯kHzâ¯+â¯70â¯kHz) indicate dual-frequency ultrasound can significantly enhance the cavitation effect.
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Ultrasonic vibration honing technology is an effective means for materials difficult to machine, where cavitation occurs in grinding fluid under the action of ultrasound. To investigate the changes of single cavitation bubble characteristics in the grinding area and how honing parameters influence bubble characteristics, a dynamic model of single cavitation bubble in the ultrasonic vibration honing grinding area was established. The model was based on the bubble dynamics and considered the condensation and evaporation of kerosene steam and honing processing environment. The change rules of bubble radius, temperature, pressure and number of kerosene steam molecules inside the bubble were numerically simulated in the process of bubble moving. The results show that the condensation and evaporation of kerosene steam can help to explain the changes of temperature and pressure inside the bubble. Compared with ultrasonic vibration, the amplitude of bubble radius is greatly suppressed in the ultrasonic honing environment. However, the rate of movement of the bubble is faster. Meanwhile, the minimum values of pressure and temperature are larger, and the number of kerosene steam molecules is less. By studying the effect of honing factors on the movement of the cavitation bubble, it is found that honing pressure has a greater influence on bubble evolution characteristics, while rotation speed of honing head has a minor effect and the reciprocating speed of honing head has little impacts.
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The bubble collapse near a wall will generate strong micro-jet in a liquid environment under ultrasonic field. To explore the effect of the impact of near-wall acoustic bubble collapse micro-jet on an aluminum 1060 sheet, the cavitation threshold formula and micro-jet velocity formula were first proposed. Then the Johnson-Cook rate correlation material constitutive model was considered, and a three-dimensional fluid-solid coupling model of micro-jet impact on a wall was established and analyzed. Finally, to validate the model, ultrasonic cavitation test and inversion analysis based on the theory of spherical indentation test were conducted. The results show that cavitation occurs significantly in the liquid under ultrasonic field, as the applied ultrasonic pressure amplitude is much larger than liquid cavitation threshold. Micro pits appear on the material surface under the impact of micro-jet. Pit depth is determined by both micro-jet velocity and micro-jet diameter, and increases with their increase. Pit diameter is mainly related to the micro-jet diameter and dp/dj≈0.95-1.2, while pit's diameter-to-depth ratio is mainly negatively correlated with the micro-jet velocity. Wall pressure distribution is mostly symmetric and its maximum appears on the edge of micro-jet impingement. Obviously, the greater the micro-jet velocity is, the greater the wall pressure is. Micro pits formed after the impact of micro-jet on aluminum 1060 surface were assessed by ultrasonic cavitation test. Inversion analysis results indicate that equivalent stress, equivalent strain of the pit and impact strength, and velocity of the micro-jet are closely related with pit's diameter-to-depth ratio. For the pit's diameter-to-depth ratio of 16-68, the corresponding micro-jet velocity calculated is 310-370m/s.