Copper recovery from low-grade copper sulfides using bioleaching and its community structure succession in the presence of Sargassum.

Bioleaching characteristics and bacterial community structure were studied during low-grade copper sulfide ores bioleaching in the presence of pretreated Sargassum (PSM). Results indicated that proportion of attached bacteria and copper recovery were improved by using appropriate-dosage PSM. High copper recovery (82.99%) and low Fe3+ concentration were obtained when 150 mg L-1 PSM was used. Precipitation, such as KFe3(SO4)2(OH)6 and (H3O)Fe3(SO4)2(OH)6, was not found in samples used PSM according to XRD, FTIR and TG analyses, which may result from less passivation layer formed by Fe3+ hydrolysis. I- contained in PSM can act as the reductant to convert Fe3+ into Fe2+, which can reduce Fe3+ hydrolysis and adjust Eh value. Bacterial community structure was influenced significantly by PSM according to the 16 S rDNA analysis. Acidithiobacillus ferrooxidans dominated proportion of bacterial community throughout bioleaching process, whose proportion reached 89.1091% after 14 days in sample added 150 mg L-1 PSM.

Mechanical properties and energy damage evolution mechanism of fiber-reinforced cemented sulfur tailings backfill under uniaxial compression.

This paper studies mechanical properties and energy damage evolution of fiber-reinforced cemented sulfur tailings (CSTB) backfill. The effects of fiber length and fiber content on the stress, toughness and failure properties of the CSTB were systematically revealed. In addition, the energy index evolution law was studied, and the energy damage evolution mechanism of CSTB was revealed. The results show that the deformation failure of fiber-reinforced CSTB mainly goes through four stages: initial crack compaction, linear elastic deformation, yield failure and post-peak failure. The peak stress and residual stress of the CSTB firstly increase and then decrease with the increase of fiber content and the addition of fiber can promote the change from brittle failure to ductile failure of the CSTB. Adding appropriate amount of fiber can improve the toughness of CSTB, and the influence degree of fiber length on the toughness index of CSTB is 6mm>12mm>3mm. The total strain energy increases linearly along the variation of fiber content, while the elastic strain energy and dissipated energy increase exponentially at the peak stress point. In the process of CSTB deformation and failure, "gentle-linear growth-slow growth-rapid decline" is for elastic strain energy, while "gentle-slow growth-rapid growth-linear growth" is for dissipation energy. The damage and failure of CSTB mainly experienced four stages: initial damage, slow growth of damage, accelerated damage and damage failure, and the damage evolution curve also showed the changing characteristics of "gentle-slow growth-rapid growth-linear growth". The CSTB without added fiber showed obvious "Y-type" and "linear-type" shear failure characteristics and the phenomenon of shear cracks penetrating the backfill appeared. No big shear crack occur when it is damaged, showing that the fiber addition restrain the crack growth and improve the overall crack resistance of the CSTB. Hydration products are obviously distributed on the surface of the fiber, which indicates that the fiber will be evenly dispersed in the CSTB and form a certain bonding force with the cement-tailings matrix, thus improving the overall mechanical properties of the CSTB.

Experimental study on time-dependent rheological properties and mechanical performance of cemented fine tailings backfill mixed with polypropylene fiber.

By preparing fine tailings slurry with different mass concentration and fiber content, the rheological parameters of slurry with different fiber content and curing time were tested. In addition, the influence law of fiber content and curing time on compressive strength was analyzed through the prepared fine tailings backfill samples, and the microstructure characteristics of fine tailings backfill were further studied. The results show that when the fiber content is 0.2 ~ 1.2%, the yield stress and plastic viscosity of the slurry increase with the increase of fiber content, and the thixotropy of the slurry also shows the same change characteristics. The bridge effect of fiber makes it easier for forming network structure, which increase the slurry rheology. When the curing time ranges from 0 h to 2.5 h, the increasing of curing time leads to the increasing trend of rheological parameters, and also increases the thixotropy of slurry. However, the increase of rheological parameters will continuously decrease when the curing time exceeds 1 h, indicating that the influence of curing time on yield stress and thixotropy will gradually weaken with the continuous extension of curing time. When the curing age increases from 3 to 56 days, the compressive strength of the fine tailings backfill increases with the curing age, but the increasing range of compressive strength decreases gradually. When the fiber content ranges from 0.2 to 1.2%, the compressive strength of backfill increases first and then decreases with the increase of fiber content, and reaches the maximum value when the fiber content is 0.6%. The extension of curing time reduces the generation of large-scale pore structure, which promotes the formation of more compact microstructure of backfill.

The Ratio Optimization and Strength Mechanism of Composite Cementitious Material with Low-Quality Fly Ash.

To resolve the limited large-scale methods of disposal of low calcium fly ash with poor activity, based on the double excitation principle, clinker and desulfurized gypsum are used as alkali/salt activators to activate fly ash and slag, avoiding the inconvenience of strong alkali activating fly ash in industry. Firstly, the strength test of a filling body with multiple ratio composite cementing material is carried out, and the weight coefficient of each material to strength is analyzed by grey correlation degree. The composition of the hydration products, microstructure, and pore structure of the filling body was analyzed by X-ray diffractometer, scanning electron microscope, thermogravimetric test, and mercury compression test. The strength mechanism of the cemented body was confirmed. The results show that cemented backfill prepared by composite cementitious material, which contained high content and low-quality fly ash, can meet the strength requirements of subsequent backfill in a mine. The degree of composite cementitious material influence on 7 d strength is slag > desulfurized gypsum > fly ash > clinker; the degree of influence on 28 d strength is: fly ash > slag > desulfurized gypsum > clinker. The main hydration products of the composite cementable material with high content low-quality fly ash are C−S−H gel and ettringite, and the unreacted fly ash particles can still be seen at 28 d. As the curing age grows, the difference in the number of hydration products under different proportioning conditions has a weaker effect on the strength, while the influence of raw materials and product morphology on the pore structure determines the development trend of the strength. Therefore, the threshold pore size can be used to characterize the strength advantages and disadvantages reasonably.

Effect of mixed bacteria on cemented tailings backfill: Economic potential to reduce binder consumption.

Tailings used as backfilling material in the presence of mixed bacteria are discussed, and the relationship between mixed bacteria and compressive strength, size variation, water-holding capacity is analyzed in this study. The results illustrate a strong improving response of mixed bacteria with enhanced compressive strength, small size variation and low water-holding capacity of cemented tailings backfill (CTB) specimens. The binder dosage and mixed bacteria proportion have great influence on CTB specimens, which indicate that with the increase of mixed bacteria proportion and binder dosage, compressive strength increased obviously. The maximum compressive strength (4.01 MPa) is obtained in the presence of 100.00% mixed bacteria in contrast to only 2.79 MPa in its absence. Samples added high mixed bacteria proportion yield low water-holding capacity and small size variation. 16S rDNA analysis illustrates that bacteria community is influenced significantly during experiment. Further, possible reaction mechanism is proposed suggesting the possible role of mixed bacteria as promoter to form precipitation (KFe3(SO4)2(OH)6, (NH4)Fe3(SO4)2(OH)6 and (KH3O)4Fe3(SO4)2(OH)6), which reduces tiny cracks in CTB specimens. The technique of using mixed bacteria to reduce binder consumption in this study shows economic benefits to some extent.

Bioleaching of copper sulfides using mixed microorganisms and its community structure succession in the presence of seawater.

The bacterial diversity and dynamics in the leaching solution were analyzed during bioleaching of low-grade copper sulfide ore in the presence of seawater in this study. The results indicated a promoting response of appropriate-proportion seawater to bioleaching with improved copper recoveries. A maximum of 84.70% copper recovery was obtained in the presence of 20.00% seawater in contrast to only 72.49% in its absence. The experiments verified that seawater owned a great influence on Attached bacteria and bacterial species. 16S rDNA analysis illustrated that bacterial species decreased distinctly in the presence of seawater. Little difference between blank sample (no seawater) and sample adding 20.00% seawater was indicated by beta diversity index. Bacteria (including Acidithiobacillus ferrooxidans, Sphingomonas leidyi and Lactobacillus acetotolerans) were influenced significantly after adding seawater. Acidithiobacillus ferrooxidans accounted for the highest proportion of the community whether seawater was added or not during bioleaching.

Bacterial-mediated recovery of copper from low-grade copper sulphide using acid-processed rice straw.

Bacteria community and copper recovery in presence of acid-processed rice straw (ARW) were explored during low-grade copper sulphide bioleaching. The results indicated a strongly promoting response of appropriate-quality ARW with improved bacteria concentration and enhanced copper recovery. The highest bacteria concentration reached 9.54 × 107 cells·mL-1 with an increase by 69.15%. And a maximum of 95.32% copper leaching rate with a relatively low Fe3+ concentration (329.00 mg·L-1) was obtained in presence of 1.0 g powdered ARW compared to only 83.40% in its absence. That is due to less development of passivation layer formed by Fe3+ hydrolysis, which is contributed by reducing ARW. 16S rDNA analysis illustrated the dominant leaching bacteria (Acidithiobacillus ferrooxidans) was influenced significantly, whose proportion reached 40.38% to the total bacteria when the ARW was added compared to 15.92% in its absence. And Stenotrophomonas accounted for the highest proportion of the bacteria community throughout bioleaching process.

Technological assessment of a mining-waste dump at the Dexing copper mine, China, for possible conversion to an in situ bioleaching operation.

In order to extract copper metal from the waste dump of Dexing copper mine and resolve the environmental problems caused by acidic water and heavy metals, a dump bioleaching plant was designed based on a series of experimental investigations. The investigation shown that the low-grade of the dump, refractoriness of chalcopyrite, leakage of pad, small Acidithiobacillus population and low dump permeability are the main factors that contribute to the challenges faced by the plant. Stability of the high and steep slope of the dump is the other hidden danger to which much attention is not paid. To evaluate the potential unstability of the dump, the leaching process, ore surface erosion, particle size, chemical elements and mechanical properties of the waste rock in DCM were investigated through experiment in this paper.