Development and forensic validation of human genomic DNA quantification kit.

DNA quantification is an essential step for successful multiplex short tandem repeat (STR) polymerase chain reactions (PCR), which are used for confirming identities using human genomic DNA. The new DNA quantification kit, named the National Forensic Service Quantification (NFSQ) kit, simultaneously provides total human DNA concentration, human male DNA concentration, and a DNA degradation index (DI) using multiplex TaqMan fluorescent probes. The NFSQ was validated according to developmental validation guidelines from the SWGDAM and MIQE. NFSQ detected up to 0.00128 ng/µL and could detect male DNA up to a 1:8000 ratio of male to female DNA. In PCR inhibitor tests, NFSQ could measure DNA at a concentration of 200 ng/µL of humic acid and 600 µM of hematin. The NFSQ kit showed a DI value trend similar to other qPCR kits. In the reproducibility study, the coefficient of variation of the NFSQ kit was within 10%. The quantitative results of the casework samples obtained using the NFSQ kit were consistent with the STR interpretation results. The NFSQ kit can be useful in the human identification process, as it has detection capabilities similar to those of other comparable quantification kits.

A numerical study on the ship-bank interaction at various water depths of a surface ship.

Ship maneuvering in restricted waters is a significant challenge in navigation safety due to the complex flow around the ship. In particular, when a ship travels close to a lateral bank and shallow water, the hydrodynamic interaction forces significantly influence the maneuvering motion of the ship. Maneuverability in restricted water is even more difficult for an autonomous surface ship. Therefore, it is necessary to assess the effects of maneuvering near a bank and in shallow water for an autonomous surface ship. In this study, maneuvering simulations considering the bank effect at various water depths are implemented based on hydrodynamic forces estimated using computational fluid dynamics simulation. First, virtual captive model tests at various water depths and simulations of various lateral distances to the banks are performed to estimate the hydrodynamic forces using computational fluid dynamics simulation. The simulation method is validated by comparing the simulation results of the static drift test in deep water with the measured one in the experimental method. Second, the maneuvering simulations for the turning circle test and zig-zag test at various water depths are conducted using the obtained hydrodynamic coefficients. Then, the maneuvering simulations in deep water are compared with the experiment results, and a good agreement is observed. Finally, the simulation considering the bank effects at various water depths is evaluated and discussed.

Experimental and numerical simulation on dynamics of a moored semi-submersible in various wave directions.

A moored floating platform has great potential in ocean engineering applications because the mooring system is necessary to keep a floating platform in the station. It relates directly to operational efficiency and safety of a floating platform. This study presents a comprehensive assessment of the dynamics of a moored semi-submersible in waves by performing model test and numerical simulation. First, a three-dimensional panel method was used to estimate the motion of a moored semi-submersible in waves. A semi-submersible is modelled as a rigid body with six degrees-of-freedom (6DOF) motion. Dynamic response analysis of a semi-submersible is performed in regular wave and irregular wave. Second, the model test is performed in various wave directions. An Optical-based system is used to measure 6DOF motion of a semi-submersible. Numerical results are compared with the experimental results in various wave directions. Wavelength and wave direction showed significant effects on the motion response of a semi-submersible in regular wave. Third, to obtain a better understanding of response frequencies, the time histories of motion responses in irregular wave are converted from the time domain to the frequency domain. Effects of the wave frequency component on motion responses and mooring dynamics are analyzed. Motion spectrum in irregular wave has a strong response to the natural frequency of a moored semi-submersible and the peak of wave frequency. Finally, exceedance probability is estimated to predict probable extreme values of motion responses of a moored semi-submersible as well as mooring dynamics.