Miniature Two-Photon Microscopic Imaging Using Dielectric Metalens.

Miniature two-photon microscopy has emerged as a powerful technique for investigating brain activity in freely moving animals. Ongoing research objectives include reducing probe weight and minimizing animal behavior constraints caused by probe attachment. Employing dielectric metalenses, which enable the use of sizable optical components in flat device structures while maintaining imaging resolution, is a promising solution for addressing these challenges. In this study, we designed and fabricated a titanium dioxide metalens with a wavelength of 920 nm and a high aspect ratio. Furthermore, a meta-optic two-photon microscope weighing 1.36 g was developed. This meta-optic probe has a lateral resolution of 0.92 µm and an axial resolution of 18.08 µm. Experimentally, two-photon imaging of mouse brain structures in vivo was also demonstrated. The flat dielectric metalens technique holds promising opportunities for high-performance integrated miniature nonlinear microscopy and endomicroscopy platforms in the biomedical field.

High-power, sub-100-fs, 1600-1700-nm all-fiber laser for deep multiphoton microscopy.

The 1600-1700-nm ultrafast fiber lasers attract great interests in the deep multiphoton microscopy, due to the reduced levels of the tissue scattering and absorption. Here, we report on the 86.7-MHz, 717-mW, 91.2-fs, all-fiber laser located in the spectral range from 1600 nm to 1700nm. The soliton self-frequency shift (SSFS) was introduced into the Er:Yb co-doped fiber amplifier (EYDFA) to generate the high-power, 1600-1700-nm Raman soliton. Detailed investigations of the nonlinear fiber amplification process were implemented in optimizing the generated Raman soliton pulses. The miniature multiphoton microscopy was further realized with this home-built laser source. The clearly imaging results can be achieved by collecting the generated harmonic signals from the mouse tail skin tissue with a penetration depth of ∼500 µm. The experimental results indicate the great potential in utilizing this 1600-1700-nm fiber laser in the deep multiphoton microscopy.

Experimental and Modeling Analysis of Polypropylene Fiber Reinforced Concrete Subjected to Alkali Attack and Freeze-Thaw Cycling Effect.

The durability of concrete materials in harsh environmental conditions, particularly in cold regions, has garnered significant attention in civil engineering research in recent years. Concrete structures in these areas are often damaged by the combined effects of alkali-silica reaction (ASR) and freeze-thaw cycles, leading to structural cracks and significant safety hazards. Numerous studies have demonstrated that polypropylene fiber concrete exhibits excellent crack resistance and durability, making it promising for applications in cold regions. This study elucidates the impact of alkali content on concrete durability by comparing the mechanical properties and durability of different alkali-aggregate concretes. The principal experimental methodologies employed include freeze-thaw cycle experiments, which examine patterns of mass loss; fluctuations in the dynamic modulus of elasticity; and changes in mechanical properties before and after freeze cycles. The findings indicate that increased alkali content in concrete reduces its strength and durability. At 100% alkali-aggregate content, compressive strength decreases by 35.5%, flexural strength by 32.9%, mass loss increases by 35.85%, relative dynamic elastic modulus by 39.4%, and residual strength by 97.28%, indicating higher alkali content leads to diminished durability. Additionally, this paper introduces a constitutive damage model, validated by a strong correlation with experimental stress-strain curves, to effectively depict the stress-strain relationship of concrete under varying alkali contents. This research contributes to a broader understanding of concrete durability in cold climates and guides the selection of materials for sustainable construction in such environments.