RESUMO
Nearly one-third of the Peruvian population burns biofuels for cooking. Similarly, approximately one-third of this population lives at high altitudes. Cooking with biofuels and living at high altitudes have been implicated in adverse perinatal outcomes. This study attempted to determine the risk of low birth weight (LBW) and/or preterm delivery in relation to biofuel use in inhabitants at high altitudes in Peru. A matched-case study was performed in two high-altitude cities. All subjects (n=190) were identified from public-hospital records. Cases were matched by hospital by week of birth and area of residence. Cases were defined as: women with an at-term but LBW newborn; or women with a preterm birth. Adjusted conditional logistic regression analyses were used to determine odds ratio. Fifty-two percent of the cases used biofuel whereas only 30% of the controls used biofuel. The use of biofuel for cooking was found to be strongly associated with increased risks of LBW, but was not significantly linked to preterm births.
Assuntos
Poluição do Ar em Ambientes Fechados/efeitos adversos , Altitude , Biocombustíveis/efeitos adversos , Recém-Nascido de Baixo Peso , Nascimento Prematuro/epidemiologia , Nascimento Prematuro/etiologia , Adolescente , Adulto , Estudos de Casos e Controles , Eletricidade/efeitos adversos , Feminino , Registros Hospitalares , Humanos , Recém-Nascido , Querosene/efeitos adversos , Modelos Logísticos , Pessoa de Meia-Idade , Peru/epidemiologia , Gravidez , Resultado da Gravidez , Propano/efeitos adversos , Fatores de Risco , Adulto JovemRESUMO
Previous studies have shown that black variety of maca has beneficial effects on learning and memory in experimental animal models. The present study aimed to determine whether the hydroalcoholic extract of black maca (BM) showed a dose-response effect in mice treated with ethanol 20% (EtOH) as a model of memory impairment. Mice were divided in the following groups: control, EtOH, ascorbic acid (AA) and 0.125, 0.25, 0.50 and 1.00 g/kg of BM plus EtOH. All treatments were orally administered for 28 days. Open field test was performed to determine locomotor activity and water Morris maze was done to determine spatial memory. Also, total polyphenol content in the hydroalcoholic extract of BM was determined (0.65 g pyrogallol/100 g). Mice treated with EtOH took more time to find the hidden platform than control during escape acquisition trials; meanwhile, AA and BM reversed the effect of EtOH. In addition, AA and BM ameliorated the deleterious effect of EtOH during the probe trial. Correlation analyses showed that the effect of BM a dose-dependent behavior. Finally, BM improved experimental memory impairment induced by ethanol in a dose-response manner due, in part, to its content of polyphenolic compounds.
Assuntos
Etanol/toxicidade , Lepidium/química , Transtornos da Memória/induzido quimicamente , Extratos Vegetais/farmacologia , Animais , Relação Dose-Resposta a Droga , Reação de Fuga/efeitos dos fármacos , Masculino , Transtornos da Memória/tratamento farmacológico , Camundongos , Extratos Vegetais/química , Fatores de TempoRESUMO
Inorganic arsenic exposure has been linked to the development of several health conditions, including adverse birth outcomes; and around 150 million of people worldwide are exposed to levels above the WHO suggested limit of 10 µg/L. A recent risk assessment in pregnant women of Tacna, of this same population performed by our group, found that 70.25% were exposed to arsenic concentrations in drinking water ≥25 µg/L. The present study aimed to evaluate the relationship between prenatal total urinary arsenic (U-tAs) and inorganic arsenic (U-iAs) with adverse birth outcomes. A total of 147 pregnant women from the province of Tacna, Peru, during February - March, 2019, were evaluated for U-tAs and U-iAs exposure during their second trimester of pregnancy, while the birth records of their children were collected from the local hospital. The geometric mean U-tAs was 43.97 ± 25.88 µg/L (P50 22.30, range 5.99 - 181.94 µg/L) and U-iAs was 5.27 ± 2.91 µg/L. Controlling for maternal age, pre-pregnancy BMI, parity, mother's education and newborn sex, no relationship was observed between tertile of U-tAs and the birth outcomes considered, although we found an apparent but statistically non-significant dose-response relationship for small-for-gestational-age 2.38% ( 95% CI 0.003, 0.16), versus 7.32% (95% CI 0.02, 0.21%), versus 8.57% (0.03, 0.25%). This finding requires further evaluation considering other factors such as metabolic arsenic species, additional maternal covariates and ethnicity.
RESUMO
BACKGROUND: Organophosphates are broad class of chemicals widely used as pesticides throughout the world. We performed a cross-sectional study of associations between dialkylphosphate metabolites of organophosphates and semen quality among pesticide applicators in Majes (Arequipa), Peru. METHODS: Thirty-one men exposed to organophosphate (OP) pesticides and 31 non-exposed were recruited (age, 20-60 years). In exposed subjects, semen and a blood sample were obtained one day after the last pesticide application. Subjects were grouped according to levels of OP metabolites in urine. Semen samples were analyzed for sperm concentration, percentage of sperm motility, percentage of normal morphology, semen leucocytes and concentrations of fructose and zinc. Exposure to OP was assessed by measuring six urinary OP metabolites (dimethyl and diethyl phosphates and thiophosphates) by gas chromatography using a single flame photometric detector. RESULTS: Diethyldithiophosphate (p = 0.04) and diethylthiophosphate (p = 0.02) better reflected occupational pesticide exposure than other OP metabolites. Semen analysis revealed a significant reduction of semen volume and an increase in semen pH in men with OP metabolites. Multiple regression analysis showed that both occupational exposure to pesticides and the time of exposure to pesticides were more closely related to alterations in semen quality parameters than the single measurement of OP metabolites in urine. CONCLUSION: The study demonstrated that occupational exposure to OP pesticides was more closely related to alterations in semen quality than a single measurement of urine OP metabolites. Current measurement of OP metabolites in urine may not reflect the full risk.
Assuntos
Exposição Ocupacional/efeitos adversos , Intoxicação por Organofosfatos , Compostos Organofosforados/urina , Praguicidas/intoxicação , Sêmen/efeitos dos fármacos , Adulto , Estudos de Casos e Controles , Estudos Transversais , Humanos , Masculino , Pessoa de Meia-Idade , Peru , Praguicidas/urina , Análise de Regressão , Análise do Sêmen , Adulto JovemRESUMO
Rats were treated with 0, 8, 16 and 24 mg/kg of lead acetate (LA) (i.p.) for 35 days with or without Maca. Maca was co-administrated orally from day 18 to day 35. The lengths of stages of the seminiferous epithelium were assessed by transillumination. Also, sex organ weights, testicular and epididymal sperm count, sperm motility, daily sperm production, sperm transit rate and serum testosterone levels were measured. Lead acetate treatment resulted in a dose-response reduction of lengths of stages VIII and IX-XI, and serum testosterone levels. However, rats treated with 8 and 16 mg/kg but not 24 mg/kg of lead acetate showed a low number of testicular spermatids, low daily sperm production (DSP) and low epididymal sperm count. Administration of Maca to rats treated with lead acetate resulted in higher lengths of stages VIII and IX-XI with respect to lead acetate-treated rats. Moreover, treatment with Maca to lead acetate-treated rats resulted in lengths of stages VIII and IX-XI similar to the control group. Maca administration also reduced the deleterious effect on DSP caused by lead acetate treatment. Maca prevented LA-induced spermatogenic disruption in rats and it may become in a potential treatment of male infertility associated with lead exposure.
Assuntos
Lepidium/química , Compostos Organometálicos/antagonistas & inibidores , Compostos Organometálicos/toxicidade , Reprodução/efeitos dos fármacos , Animais , Epididimo/citologia , Genitália Masculina/efeitos dos fármacos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Extratos Vegetais/farmacologia , Ratos , Ratos Sprague-Dawley , Contagem de Espermatozoides , Motilidade dos Espermatozoides/efeitos dos fármacos , Testosterona/sangueRESUMO
Lepidium meyenii (Brassicaceae), known as Maca, is a Peruvian hypocotyl that grows exclusively between 4000 and 4500 m above sea level in the central Andes. Maca is traditionally employed in the Andean region for its supposed fertility-enhancing properties. The study aimed to test the hypothesis that different ecotypes of Maca (Red, Yellow and Black) after short-term (7 days) and long-term (42 days) treatment affects differentially spermatogenesis adult rats. After 7 days of treatment with Yellow and Red Maca, the length of stage VIII was increased (P<0.05), whereas with Black Maca stages II-VI and VIII were increased (P<0.05). Daily sperm production (DSP) was increased in the group treated with Black Maca compared with control values (P<0.05). Red or Yellow Maca did not alter DSP and epididymal sperm motility was not affected by treatment with any ecotype of Maca. After 42 days of treatment, Black Maca was the only ecotype that enhanced DSP (P<0.05). Moreover, Black Maca was the only that increased epididymal sperm motility (P<0.05). In relation to the control group, Red Maca did not affect testicular and epididymal weight nor epididymal sperm motility and sperm count; however, prostate weight was reduced (P<0.05). Black or Yellow Maca did not affect prostate weight. In conclusion, there were differences in the biological response of the three ecotypes of Maca (Yellow, Red and Black). Black Maca appeared to have more beneficial effect on sperm counts and epididymal sperm motility.
Assuntos
Fertilidade/efeitos dos fármacos , Lepidium/classificação , Extratos Vegetais/farmacologia , Espermatogênese/efeitos dos fármacos , Administração Oral , Animais , Hipocótilo , Masculino , Tamanho do Órgão , Peru , Extratos Vegetais/administração & dosagem , Próstata/efeitos dos fármacos , Próstata/patologia , Ratos , Ratos Sprague-Dawley , Contagem de Espermatozoides , Motilidade dos Espermatozoides , Espermatozoides/efeitos dos fármacos , Fatores de TempoRESUMO
To determine the association between occupational pesticide exposure and semen quality among sprayers using organophosphate (OPs) pesticides, 31 pesticide sprayers and 80 men who were not exposed were studied. Semen and blood samples were obtained one day after last pesticide application. Semen samples were analyzed for sperm concentration, percentage of sperm motility, percentage of normal morphology, and fructose and zinc concentrations. Blood samples were analyzed for sex hormones. Pesticide sprayers had significantly reduced age-adjusted seminal volume, percentage of motility, percentage of sperm with normal morphology, serum luteinizing hormone, serum testosterone levels, and seminal zinc concentration (a marker of prostate function), as well as significantly increased time of liquefaction, seminal pH, percentage of immature sperm morphology, and leukocyte concentration. These findings provide further evidence that occupational exposures to OP pesticides adversely affect semen quality and sex hormones.
Assuntos
Agricultura , Hormônio Luteinizante/sangue , Sêmen , Testosterona/sangue , Estudos Transversais , Coleta de Dados , Humanos , Masculino , PeruAssuntos
Arsênio , Água Potável , Arsênio/análise , Peso ao Nascer , Água Potável/análise , Feminino , Humanos , Peru/epidemiologia , Gravidez , GestantesRESUMO
BACKGROUND: Epidemiological studies have found that consumption of cruciferous vegetables is associated with a reduced risk of prostate cancer. This effect seems to be due to aromatic glucosinolate content. Glucosinolates are known for have both antiproliferative and proapoptotic actions. Maca is a cruciferous cultivated in the highlands of Peru. The absolute content of glucosinolates in Maca hypocotyls is relatively higher than that reported in other cruciferous crops. Therefore, Maca may have proapoptotic and anti-proliferative effects in the prostate. METHODS: Male rats treated with or without aqueous extracts of three ecotypes of Maca (Yellow, Black and Red) were analyzed to determine the effect on ventral prostate weight, epithelial height and duct luminal area. Effects on serum testosterone (T) and estradiol (E2) levels were also assessed. Besides, the effect of Red Maca on prostate was analyzed in rats treated with testosterone enanthate (TE). RESULTS: Red Maca but neither Yellow nor Black Maca reduced significantly ventral prostate size in rats. Serum T or E2 levels were not affected by any of the ecotypes of Maca assessed. Red Maca also prevented the prostate weight increase induced by TE treatment. Red Maca administered for 42 days reduced ventral prostatic epithelial height. TE increased ventral prostatic epithelial height and duct luminal area. These increases by TE were reduced after treatment with Red Maca for 42 days. Histology pictures in rats treated with Red Maca plus TE were similar to controls. Phytochemical screening showed that aqueous extract of Red Maca has alkaloids, steroids, tannins, saponins, and cardiotonic glycosides. The IR spectra of the three ecotypes of Maca in 3800-650 cm (-1) region had 7 peaks representing 7 functional chemical groups. Highest peak values were observed for Red Maca, intermediate values for Yellow Maca and low values for Black Maca. These functional groups correspond among others to benzyl glucosinolate. CONCLUSIONS: Red Maca, a cruciferous plant from the highland of Peru, reduced ventral prostate size in normal and TE treated rats.
Assuntos
Lepidium , Extratos Vegetais/farmacologia , Próstata/efeitos dos fármacos , Animais , Células Epiteliais/ultraestrutura , Estradiol/sangue , Lepidium/química , Lepidium/classificação , Masculino , Tamanho do Órgão/efeitos dos fármacos , Extratos Vegetais/química , Próstata/anatomia & histologia , Próstata/citologia , Ratos , Glândulas Seminais/anatomia & histologia , Glândulas Seminais/efeitos dos fármacos , Testosterona/análogos & derivados , Testosterona/sangue , Testosterona/farmacologiaRESUMO
AIM: To observe the effect of the aqueous extract of hypocotyls of the plant Lepidium meyenii (Maca) on spermatogenic damage induced by the organophosphate insecticide malathion in mice. METHODS: Mice were treated with 80 mg/kg of malathion in the presence or absence of an aqueous extract of Maca, which was orally administered 7, 14 or 21 days after injection of the malathion. Stages of the seminiferous epithelium were assessed by transillumination on days 0, 7, 14 and 21. RESULTS: The administration of Maca increased significantly the length of stage VIII on days 7, 14 and 21 of treatment compared with the controls. An increase in the length of stage IX occurred on day 14 of treatment. Malathion affected spermatogenesis by reducing the lengths of stage IX on day 7, stages VII and IX-XI on day 14 and a recovery of stages IX-XII on day 21. The magnitude of alteration in the length of stage IX produced by malathion was significantly reduced by Maca on days 7 and 14. The length of stage VIII was increased when Maca was administered to mice treated with malathion. Assessment of the relative length of stages of the seminiferous epithelium showed that Maca treatment resulted in rapid recovery of the effect of malathion. CONCLUSION: Maca enhances spermatogenesis following spermatogenic damage caused by the organophosphorous pesticide.
Assuntos
Lepidium , Malation/efeitos adversos , Fitoterapia , Espermatogênese/efeitos dos fármacos , Animais , Esquema de Medicação , Hipocótilo , Masculino , Camundongos , Extratos Vegetais/uso terapêutico , Fatores de Tempo , Resultado do TratamentoRESUMO
OBJECTIVE: To evaluate the effect of different fractions of Black Maca (Lepidium meyenii), obtained from the hydroalcoholic extract, on spermatogenesis. DESIGN: Animal study. SETTING: Animal and laboratory facilities at a university. ANIMAL(S): Forty two adult male rats from the Holtzman strain (3 months old). INTERVENTION(S): Hydroalcoholic extract of Black Maca was partitioned with the following solvents: petroleum ether, chloroform, ethyl acetate, n-butanol, and water to obtain each fraction. Forty-two rats were divided in different groups according the fraction administered and vehicle. The hydroalcoholic extract of Black Maca and its fractions and vehicle were given orally by gavage for 7 days. MAIN OUTCOME MEASURE(S): Daily sperm production, epididymal sperm count, and sperm count in the vas deferens. RESULT(S): Daily sperm production was higher in the ethyl acetate group compared with all other groups. The epididymal sperm count was higher in rats treated with ethyl acetate fraction compared with rats treated with vehicle (control), petroleum ether, n-butanol, or water fractions. The sperm count in vas deferens was lower in rats treated with ethyl acetate, petroleum ether, or water fractions compared with the control group; thus, the sperm count in vas deferens in rats treated with chloroform and n-butanol fractions was higher than in the petroleum ether group. CONCLUSION(S): The greatest effect on spermatogenesis was observed in the ethyl acetate fraction from the hydroalcoholic extract of Black Maca, suggesting that the compounds related to the beneficial effect on sperm production of Black Maca are presented in this fraction. Antioxidant components could play a role in the effect of increased epididymal sperm concentration observed in the model.
Assuntos
Lepidium , Extratos Vegetais/farmacologia , Testículo/efeitos dos fármacos , Fatores Etários , Animais , Peso Corporal/efeitos dos fármacos , Fracionamento Químico , Avaliação Pré-Clínica de Medicamentos , Etanol/farmacologia , Lepidium/química , Masculino , Tamanho do Órgão/efeitos dos fármacos , Extratos Vegetais/química , Ratos , Ratos Sprague-Dawley , Contagem de Espermatozoides , Espermatogênese/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Espermatozoides/metabolismo , Testículo/anatomia & histologia , Testículo/fisiologia , Água/farmacologiaRESUMO
BACKGROUND: Organophosphorus (OPs) pesticides are the most commonly used pesticides in Peruvian agriculture. The population at risk for OPs exposure includes formulators, applicators and farmers. Majes Valley is the most important agricultural center of the Southern region of Peru. The present study was aimed to determine the knowledge about using OPs, safety practice and urinary dialkylphosphate metabolites on OP applicators in the Majes Valley, Peru. METHODS: This study was based on a questionnaire which included socio-demographic characteristics, knowledge of safety practices to handling OPs, characteristics of pesticide application and use of protective measures to avoid pesticide contamination. Exposure was assessed by measuring six urinary OP metabolites (DMP, DMTP, DMDTP, DEP, DETP, and DEDTP) by gas chromatography using a single flame photometric detector. The sample consisted of 31 men and 2 women aged 20 - 65 years old. RESULTS: 76% of applicators had at least one urinary dialkylphosphate metabolite above the limit of detection. The geometric mean (GM) and the geometric standard deviation (GSD) of DMP and DEP were 5.73 microg/g cr. (GSD 2.51), and 6.08 microg/g cr. (GSD 3.63), respectively. The percentage of applicators with detectable DMP, DMDTP, and DMTP in urine was 72.72%, 3.03%, and 15.15%, respectively, while the corresponding figures for DEP, DETP, and DEDTP were 48.48%, 36.36% and 15.15%, respectively. There was no significant association between the use of protection practices and the absence of urine OPs metabolites suggesting inadequate protection practices. CONCLUSION: The pesticide applicators in Majes Valley have significant exposure to OP pesticides, probably due to inappropriate protective practices. Future work should evaluate possible health effects.
RESUMO
Actualmente, la exposición ocupacional a contaminantes como metales pesados y plaguicidas ha crecido por la actividad industrial, minera y agrícola. Los efectos adversos en la salud humana se presentan en el sistema respiratorio, renal, nervioso, endocrino, reproductor,siendo este último muy sensible a muchos agentes físicos y químicos generados por la actividad industrial o agrícola. Estos agentes están presentes en algunas actividades ocupacionales y en el ambiente en general. Las evidencias de estudios toxicológicos, epidemiológicos, bioquímicos y fisiológicos, demuestran que el plomo tiene efectos adversos en la salud humana de los trabajadores ocupacionalmente expuestos a diferentes concentraciones pudiendo causar infertilidad masculina. El impacto de la exposición crónica al plomo en el varón incluye reducción de la libido, alteración en la espermatogénesis (reducción en cantidad y motilidad, e incremento de formas anormalesde los espermatozoides), daño cromosómico, función prostática anormal y cambios en los niveles de testosterona. Para el caso de losplaguicidas órganofosforados (OP) hay un alto riesgo de exposición ocupacional y no ocupacional de estos químicos debido a su extenso uso en la agricultura y en el ambiente doméstico, se ha demostrado, que afectan también el sistema reproductor masculino actuando como tóxicos testiculares que causan alteraciones citotóxicas y citocinéticas reversibles en las células germinales, alteran la síntesis deandrógenos y la calidad seminal en los trabajadores expuestos a OP, sobre todo los individuos dedicados a la actividad agrícola. En la presente revisión se ha recopilado diferente información de los efectos adversos de la exposición ocupacional al plomo y a los plaguicidas órganofosforados sobre la función reproductiva.
Nowadays there is an increased occupational exposure to different pollutants such as heavy metals and pesticides, because of the significant growth in industrial, mining, and agriculture activities. Adverse effects on human health can be observed in the respiratory, renal, nervous, endocrine, and reproductive systems, being the latter quite susceptible to many physical and chemical agents generated byindustrial or agricultural activities. These agents are present in some specific activities or in the environment. Evidence from toxicological,epidemiological, biochemical, and physicioligal studies shows that lead has adverse effects on health of workers exposed to different concentrations of this metal, and the exposure may lead to male infertility. These negative effects include: libido reduction, impairedspermatogenesis (reduction in sperm number and motility, and increased number of abnormal sperm), chromosomal damage, abnormalprostate function, and changes in testosterone levels. In the case of organophosphorus pesticides (OPs), agriculture workers are at highrisk for occupational and non-occupational exposure to these chemicals, because of their extensive use in agriculture and the householdenvironment. OPs have been proven also to affect male reproductive system because of testicular toxicity leading to cytotoxic and cytokinetic reversible alterations in germinal cells, alterating androgen synthesis and quality of semen in exposed persons, particularlythose individuals working in agriculture. This review focuses on the adverse effects on male reproductive function caused by occupational exposure to lead and organophosphorus compounds.