RESUMO
Conventionally, the lateral shoots of chrysanthemum are removed manually, which is time consuming and uneconomical. The development of branchless chrysanthemum will economize its commercial cultivation. To investigate the regulatory mechanism of branchlessness, we undertook cloning of Dendranthema grandiflorum Kitamura Lateral suppressor-like (DgLsL) gene for development of lateral shoot in chrysanthemum. A full-length cDNA of DgLsL gene was isolated by screening cDNA library and performing Rapid Amplification of cDNA Ends (RACE) PCR. Phylogenetic analysis showed that the DgLsL gene is closely related to Lateral suppressor that encodes transcriptional regulator proteins belonging to GRAS family of known transcription factor. Southern blot analysis revealed that DgLsL gene in chrysanthemum genome has one copy. DgLsL expression was apparently up-regulated by ethephon treatment. The expression patterns revealed that DgLsL transcripts were detected in all organs, but showed their highest level in stems.
Assuntos
Chrysanthemum/metabolismo , Genes de Plantas , Sequência de Aminoácidos , Southern Blotting , Chrysanthemum/genética , Clonagem Molecular , Biblioteca Gênica , Dados de Sequência Molecular , Compostos Organofosforados/farmacologia , Filogenia , Reguladores de Crescimento de Plantas/farmacologia , Homologia de Sequência de Aminoácidos , Regulação para CimaRESUMO
A Gram-negative, strictly aerobic, non-spore-forming bacterium, designated strain BD-c194(T), was isolated from diesel-contaminated soil in Geoje, Korea. The cells were short, motile rods with single polar flagella. Growth of strain BD-c194(T) was observed between 15 and 45 degrees C (optimally at 30-35 degrees C) and between pH 6.0 and 9.5 (optimally at pH 7.5-9.0). The predominant fatty acids were 11-methyl C(18 : 1)omega7c, C(16 : 0), C(18 : 1)omega7c, C(18 : 0) and an unknown fatty acid (equivalent chain-length 18.814); a large amount of phosphatidylglycerol and a small amount of diphosphatidylglycerol were present as polar lipids. The G+C content of the genomic DNA was 60.8 mol% and the major isoprenoid quinone was Q-10. A comparative 16S rRNA gene sequence analysis showed that strain BD-c194(T) formed a well-defined phyletic lineage within the genus Devosia (with 100 % bootstrap support). The levels of 16S rRNA gene sequence similarity with respect to the type strains of other Devosia species ranged from 95.0 to 96.1 %. On the basis of chemotaxonomic data and molecular properties, strain BD-c194(T) represents a novel species within the genus Devosia, for which the name Devosia geojensis sp. nov. is proposed. The type strain is BD-c194(T) (=KCTC 22082(T) =DSM 19414(T)).
Assuntos
Gasolina , Hyphomicrobiaceae/classificação , Hyphomicrobiaceae/isolamento & purificação , Microbiologia do Solo , Poluentes do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/análise , DNA Ribossômico/análise , Ácidos Graxos/análise , Genes de RNAr , Genótipo , Hyphomicrobiaceae/genética , Hyphomicrobiaceae/fisiologia , Dados de Sequência Molecular , Fenótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
A Gram-negative, rod-like, stalk-producing bacterium, designated strain EMB102(T), was isolated from activated sludge that performed enhanced biological phosphorus removal in a sequencing batch reactor. Cells without stalks were motile with single polar flagella, but cells that did produce stalks were non-motile and lacked polar flagella. Growth of strain EMB102(T) was observed at temperatures between 15 and 35 degrees C (optimum, 30 degrees C) and between pH 6.0 and 9.0 (optimum, pH 7.5-8.5). The predominant fatty acids of strain EMB102(T) were C(18 : 1) omega 7c, C(16 : 0) and C(15 : 0). The predominant polar lipid was phosphatidylglycerol. The G+C content of the genomic DNA was 64.1 mol% and the major quinone was Q-10. Comparative 16S rRNA gene sequence analyses showed that strain EMB102(T) formed a distinct phyletic lineage within the genus Brevundimonas. The levels of 16S rRNA gene sequence similarity between the type strains of Brevundimonas species ranged from 95.8 to 97.5 %. DNA-DNA relatedness levels between the EMB102(T) and closely related Brevundimonas species were below 15.0 %. On the basis of chemotaxonomic data and molecular properties, strain EMB102(T) represents a novel species within the genus Brevundimonas, for which the name Brevundimonas aveniformis sp. nov. is proposed. The type strain is EMB102(T) (=KCTC 12609(T)=DSM 17977(T)).
Assuntos
Caulobacteraceae/classificação , Caulobacteraceae/isolamento & purificação , Esgotos/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , Caulobacteraceae/química , Caulobacteraceae/genética , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Flagelos , Genes de RNAr , Concentração de Íons de Hidrogênio , Locomoção , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Nomes , Hibridização de Ácido Nucleico , Fosfolipídeos/análise , Fósforo/metabolismo , Filogenia , Quinonas/análise , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , TemperaturaRESUMO
A Gram-negative bacterium, designated strain EMB117T, was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25-30 degrees C and pH 7.0-8.0. Strain EMB117T contained phosphatidylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH, iso-C(15 : 0) 3-OH and summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH). The G+C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB117T belonged to the genus Flavobacterium and was most closely related to Flavobacterium johnsoniae DSM 425(T) (97.8 % sequence similarity). The DNA-DNA relatedness between strain EMB117T and F. johnsoniae ATCC 17061T was about 18 %. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium defluvii sp. nov. is proposed. The type strain is EMB117T (=KCTC 12612T=DSM 17963T).