RESUMO
The copper phytate IP6Cu, IP6Cu2 and IP6Cu3 complexes were synthesized changing the phytate to metal mole ratio. The obtained products have been characterized by means of chemical and spectroscopic studies. Spectroscopic ATR/IR, FT-Raman, UV-Vis, EPR and magnetic measurements were carried out. The structures of these compounds have been proposed on the basis of the group theory and geometry optimization taking into account the shape and number of the bands corresponding to the stretching and bending vibrations of the phosphate group and metal-oxygen polyhedron. The role of the inter- and intra-hydrogen bonds in stabilization of the structure has been discussed. EPR studies showed that a local rhombic symmetry of copper ions appears in the studied phytates. Dominant interactions show antiferromagnetic properties depending on the content of paramagnetic ions.
Assuntos
Complexos de Coordenação/química , Cobre/química , Ácido Fítico/química , Teoria da Densidade Funcional , Espectroscopia de Ressonância de Spin Eletrônica , Modelos Moleculares , Conformação Molecular , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral RamanRESUMO
Little is known about the role of the kallikrein-kinin system in chronic spontaneous urticaria (CSU). Kallikrein 5 (KLK5), a trypsin-like enzyme, is the most abundant in the skin and plays a role in itching and inflammatory reaction. In this study, we determined plasma KLK5 concentration, and its associations with acute phase response in CSU patients. Concentrations of KLK5 in plasma and CRP in serum were measured in patients with CSU of varying severity and in the healthy subjects. Plasma KLK5 concentrations were significantly lower in CSU (all) and moderate-severe CSU patients, as compared with the controls. There were no significant differences in KLK5 concentration in mild CSU patients as compared with the healthy subjects and moderate-severe CSU patients. No correlation was observed between KLK5 and CRP concentrations in the patients. It may be considered that circulating kallikrein 5 is down-regulated in CSU patients, however its potential role and the possible underlying mechanism are unknown.
Assuntos
Calicreínas/sangue , Urticária/sangue , Adulto , Proteína C-Reativa/metabolismo , Doença Crônica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de DoençaRESUMO
Chronic spontaneous urticaria (CSU) is associated with activation of acute phase response. Questions arise regarding its association with other inflammatory mediators. To determine plasma IL-8 concentration in CSU patients and its association with C-reactive protein (CRP) concentration, a nonspecific inflammatory marker of the disease activity, concentrations of plasma IL-8 and serum CRP were measured in CSU patients and compared with healthy controls. IL-8 and CRP concentrations were significantly higher in CSU patients as compared with the healthy subjects. In addition, there were significant differences in IL-8 and CRP concentrations between CSU patients with moderate-severe symptoms and the healthy subjects. Plasma IL-8 and serum CRP concentrations showed a significant correlation with urticaria activity score (UAS). Additionally, a significant positive correlation was observed between IL-8 and CRP concentrations. Up-regulations of IL-8 and its association with the marker of clinical and inflammatory activity suggest a role of this cytokine in the pathogenesis of CSU.
Assuntos
Proteína C-Reativa/metabolismo , Interleucina-8/sangue , Urticária/diagnóstico , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Doença Crônica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Urticária/sangue , Urticária/fisiopatologiaRESUMO
BACKGROUND: Lower serum vitamin B12 concentrations have been observed in patients with chronic spontaneous urticaria (CSU). It is known that vitamin B12 deficiency is closely related to hyperhomocysteinaemia, which is associated with a proinflammatory state. AIM: To assess the relationship between vitamin B12 status and concentrations of homocysteine (Hcy) with acute phase response in patients with CSU. METHODS: Circulating concentrations of vitamin B12, Hcy and C-reactive protein (CRP) were measured in 42 patients with CSU of varying severity, and compared with 19 healthy controls (HCs). RESULTS: Significantly lower concentrations of vitamin B12 and higher concentrations of CRP were observed in the serum of the patients with CSU compared with HCs (P < 0.01 and P < 0.001, respectively). However, there were no significant differences in plasma Hcy concentrations between the investigated groups. In addition, no correlations were found between the concentrations of vitamin B12, Hcy and CRP. CONCLUSIONS: Lower values of vitamin B12 concentration in patients with CSU were not associated with higher Hcy concentrations, suggesting that such patients do not have functional vitamin B12 deficiency.
Assuntos
Homocisteína/sangue , Urticária/sangue , Vitamina B 12/sangue , Adulto , Aterosclerose/sangue , Aterosclerose/etiologia , Biomarcadores/sangue , Proteína C-Reativa/metabolismo , Doença Crônica , Feminino , Seguimentos , Humanos , Hiper-Homocisteinemia , Imunoturbidimetria , Medições Luminescentes , Masculino , Índice de Gravidade de Doença , Fatores de Tempo , Urticária/complicações , Urticária/diagnósticoRESUMO
Early immune events associated with reduced larval burden remain unclear in parasite-resistant breeds of sheep. Therefore, our objective was to determine breed differences in immune-related gene expression following infection with H. contortus. Gene expression in abomasal tissue and mucosa and in abomasal lymph nodes (ALN) was measured in 24 St. Croix (hair) lambs and 24 Dorset x (Finn-Rambouillet) (wool) lambs at 0 (uninfected), 3, 5 and 7 days after infection with 10 000 L3 H. contortus larvae. Expression of IL-4 in abomasal mucosa was detected on day 3 and increased to day 7 in hair lambs, but was not detectable in wool lambs. Genes that recruit neutrophils (CXCL1) and macrophages (MCP1) were upregulated in abomasal mucosa of hair lambs. Genes associated with alternative macrophage activation (ARG-1) and eosinophil activation (Gal-14) were also upregulated in the abomasal mucosa of hair lambs. Tissue remodeling genes (MMP13, PDGF) and tumour necrosis factor alpha (TNF-α) and MCP1 were upregulated in abomasal tissue of wool lambs; these lambs also had greater expression of forkhead box P3 in ALN. These data indicate a role for early IL-4 expression locally and demonstrate potential downregulation of immunity in wool sheep that could facilitate establishment of H. contortus.
Assuntos
Abomaso/parasitologia , Hemoncose/veterinária , Haemonchus/imunologia , Interleucina-4/genética , Doenças dos Ovinos/genética , Abomaso/imunologia , Animais , Cruzamento , Fezes/parasitologia , Hemoncose/genética , Hemoncose/imunologia , Hemoncose/parasitologia , Haemonchus/fisiologia , Interações Hospedeiro-Parasita , Interleucina-4/imunologia , Ovinos , Doenças dos Ovinos/imunologia , Doenças dos Ovinos/parasitologiaRESUMO
BACKGROUND: Activation of receptor for advanced glycation end products (RAGE) leads to the proinflammatory response and the release of its soluble form (sRAGE) which appears to function as an anti-inflammatory feedback mechanism. AIM: To determine serum sRAGE concentration in CSU patients and its association with C-reactive protein (CRP) concentration, a nonspecific inflammatory marker of the disease activity. METHODS: Concentrations of sRAGE and CRP were measured in serum of CSU patients and compared with the healthy controls. RESULTS: Serum sRAGE concentrations were significantly decreased in CSU patients, especially those more severely affected. In addition, significant inverse correlations were observed between sRAGE and CRP concentrations. CONCLUSIONS: Down-regulation of sRAGE and its association with acute phase response suggest a role for RAGE activation in the pathogenesis of CSU. It seems that lower serum sRAGE concentration may enhance the urticarial processes.
Assuntos
Reação de Fase Aguda/sangue , Receptor para Produtos Finais de Glicação Avançada/sangue , Urticária/sangue , Adulto , Proteína C-Reativa/análise , Doença Crônica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Testes CutâneosRESUMO
The phenomenon of high on-acetylsalicylic acid (ASA) treatment platelet (PLT) reactivity - HATPR - and its clinical implications have not been fully understood. Little data is available on assessing PLT activity based on the severity of intra- and postoperative bleeding in a population of orthopedic patients with normal closure time (CT) measured by a PLT function analyzer PFA-100®, despite being given long-term ASA therapy. The aim is to assess PLT function using PFA-100® in patients with ASA therapy and qualified for trauma and orthopedic surgery procedures. The retrospective analysis covered 384 patients whose PLT reactivity was assessed using PFA-100®. Out of those, 198 had been taking ASA with a 75 mg dose until hospital admission. In addition, a group of 70 patients with a proximal femoral fracture surgically treated using the dynamic hip screw (DHS) was selected, in whom severity of bleeding was assessed by HIP ASA (+). The reference group comprised 52 patients (without ASA therapy) who were operated on due to the same indications. Normal CT was found in 37% of ASA-receiving patients. Patients with normal CT, despite ASA therapy, exhibited significantly more intense bleeding after DHS surgery. A similar number of patients required red blood cells (RBCs) transfusion in HIP ASA (+) and HIP ASA (-). Increased risk of complications in HIP ASA (+) group was not found. CONCLUSIONS: Normal PLT function assessed using PFA-100® is a common phenomenon in patients with long-term ASA treatment and who are qualified for trauma and orthopedic surgery procedures. In many cases, it seems that inadequate response to ASA is only a laboratory phenomenon.
Assuntos
Aspirina/farmacologia , Plaquetas/efeitos dos fármacos , Plaquetas/metabolismo , Inibidores da Agregação Plaquetária/farmacologia , Testes de Função Plaquetária/métodos , Adulto , Idoso , Idoso de 80 Anos ou mais , Aspirina/uso terapêutico , Testes de Coagulação Sanguínea , Tomada de Decisão Clínica , Comorbidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Procedimentos Ortopédicos/efeitos adversos , Procedimentos Ortopédicos/métodos , Inibidores da Agregação Plaquetária/uso terapêutico , Testes de Função Plaquetária/normas , Cuidados Pré-Operatórios , Estudos Retrospectivos , Fatores de Risco , Ferimentos e Lesões/sangue , Ferimentos e Lesões/diagnóstico , Ferimentos e Lesões/tratamento farmacológico , Ferimentos e Lesões/cirurgia , Adulto JovemRESUMO
The main objective of this study was to examine the chronic effect of simulated intermittent normobaric hypoxia on blood antioxidant defence capacity in swimmers. The study included 14 male and 14 female competitive swimmers performing part of land training under simulated intermittent normobaric hypoxia (O2 = 15.5%) or in normoxia. Land interval training took place twice per week, with a total of 8 training units during the study, performed with individualized intensity. The activities of blood antioxidant enzymes did not change significantly during the first and last training unit in the hypoxic and normoxic group. However, when comparing individual variables a significant effect of exercise was observed on GPx an CAT activities, whereas training units significantly differentiated GPx and GR activities. The oxygen conditions and gender had a significant influence on CAT activity. The total antioxidant capacity was not significantly affected. Only in male swimmers from the hypoxic group did the training significantly increase resting levels of MDA. In conclusion, training in normobaric hypoxia was not an adequate stimulus for the excessive response of the antioxidant defence system, despite increased oxidative stress in these conditions.
RESUMO
The purpose of this study was to examine physiological and physical determinants of ice-hockey performance in order to assess their impact on the result during a selection for ice hockey. A total of 42 ice hockey players took part in the selection camp. At the end of the camp 20 best players were selected by team of expert coaches to the ice hockey team and created group G1, while the second group (G2) consisted of not selected players (non-successful group Evaluation of goodness of fit of the model to the data was based on the Hosmer Lemeshow test. Ice hockey players selected to the team were taller 181.95±4.02 cm, had lower% body fat 13.17±3.17%, a shorter time to peak power 2.47±0.35 s, higher relative peak power 21.34±2.41 W·kg(-1) and higher relative total work 305.18±28.41 J·kg(-1). The results of the aerobic capacity test showed significant differences only in case of two variables. Ice hockey players in the G1 had higher VO2max 4.07±0.31 l·min(-1) values than players in the G2 as well as ice hockey players in G1 showed a higher level of relative VO2max 51.75±2.99 ml·min(-1)·kg(-1) than athletes in G2. Ice hockey players selected to the team (G1) performed better in the 30 m Forwards Sprint 4.28±0.31 s; 6x9 Turns 12.19±0.75 s; 6x9 stops 12.79±0.49 s and Endurance test (6x30 m stops) 32.01±0.80 s than players in G2. The logistic regression model showed that the best predictors of success in the recruitment process of top level ice hockey players were time to peak power, relative peak power, VO2max and 30 m sprint forwards on ice. On the basis of the constructed predictive logistic regression model it will be possible to determine the probability of success of the athletes during following the selection processes to the team.
RESUMO
BACKGROUND: The pentraxin family plays an important role in the acute phase response to immune-inflammatory processes. The short pentraxin, C-reactive protein (CRP) is a marker of chronic spontaneous urticaria (CSU) activity, reflecting the systemic effects of inflammatory mediators associated with the disease. It is known, that the long pentraxin, pentraxin 3 (PTX3) is produced at the sites of inflammation, therefore may better reflect activity of the local inflammatory processes. To assess the relevance of PTX3 in CSU patients and its association with CRP. METHODS: Plasma PTX3 and serum CRP concentrations were measured in patients with CSU of varying severity as well as in the healthy subjects. RESULTS: The concentrations of PTX3 and CRP were significantly increased in more severe CSU patients, when compared to mild CSU and the healthy controls. There was a significant correlation between concentrations of PTX3 and CRP. CONCLUSIONS: In contrast to CRP, PTX3 is produced at the sites of inflammation, therefore it seems that elevated PTX3 may result from activation of cells involved in local urticarial processes. Finally, the correlation between these two pentraxins suggests that they may be upregulated by the same mechanisms associated with acute phase response in CSU.
Assuntos
Biomarcadores/sangue , Proteína C-Reativa/metabolismo , Componente Amiloide P Sérico/metabolismo , Urticária/sangue , Adulto , Doença Crônica , Feminino , Humanos , MasculinoRESUMO
The primary objective of this study was to determine a relationship between aerobic capacity ([Formula: see text]O2max) and fatigue from high-intensity skating in elite male hockey players. The subjects were twenty-four male members of the senior national ice hockey team of Poland who played the position of forward or defence. Each subject completed an on-ice Repeated-Skate Sprint test (RSS) consisting of 6 timed 89-m sprints, with 30 s of rest between subsequent efforts, and an incremental test on a cycle ergometer in the laboratory, the aim of which was to establish their maximal oxygen uptake ([Formula: see text]O2max). The analysis of variance showed that each next repetition in the 6x89 m test was significantly longer than the previous one (F5,138=53.33, p<0.001). An analysis of the fatigue index (FI) calculated from the times recorded for subsequent repetitions showed that the value of the FI increased with subsequent repetitions, reaching its maximum between repetitions 5 and 6 (3.10±1.16%). The total FI was 13.77±1.74%. The coefficient of correlation between [Formula: see text]O2max and the total FI for 6 sprints on the distance of 89 m (r =-0.584) was significant (p=0.003). The variance in the index of players' fatigue in the 6x89 m test accounted for 34% of the variance in [Formula: see text]O2max. The 6x89 m test proposed in this study offers a high test-retest correlation coefficient (r=0.78). Even though the test is criticized for being too exhaustive and thereby for producing highly variable results it still seems that it was well selected for repeated sprint ability testing in hockey players.
RESUMO
BACKGROUND: Our previous findings showed the importance of analysing the peripheral markers of acute phase response (APR) activation, C-reactive protein (CRP) and IL-6 in the context of urticaria activity and severity. However, these biomarkers do not reliably differentiate between APR to infectious and the disease severity. AIM: In order to investigate a possible association between the immune-inflammatory activation markers CRP and procalcitonin (PCT). METHODS: Serum PCT and CRP concentrations were measured in patients with CU of varying severity as well as in healthy subjects. RESULTS: Serum PCT and CRP concentrations were significantly increased in more severe CU patients when compared to healthy controls and mild CU, and within the CU population there was a significant correlation between concentrations of PCT and CRP. Serum PCT concentrations remained within normal ranges in most CU patients and were only slightly elevated in some severe CU cases. CONCLUSIONS: PCT serum concentration may be only slightly elevated in some cases of severe CU. Upregulation of PCT synthesis accompanied by parallel changes in CRP concentration reflects a low-grade systemic inflammatory response in CU. PCT should be considered as a better marker than CRP to distinguish between APR to infection and an active non-specific urticarial inflammation.
Assuntos
Proteína C-Reativa/metabolismo , Calcitonina/sangue , Precursores de Proteínas/sangue , Índice de Gravidade de Doença , Urticária/sangue , Adulto , Biomarcadores/sangue , Peptídeo Relacionado com Gene de Calcitonina , Estudos de Casos e Controles , Doença Crônica , Feminino , Humanos , Interleucina-6/sangue , Masculino , Regulação para Cima , Urticária/fisiopatologiaRESUMO
BACKGROUND: Active chronic urticaria, identified as a mast cell- and basophil-dependent inflammatory disorder of the skin is able to elicit acute phase response (APR). However, systemic inflammatory response in different types of urticaria is poorly characterized. AIM: To determine APR pattern in a clearly defined group of patients with acute urticaria and/or angioedema - induced by NSAIDs. METHODS: Plasma IL-6 and serum C-reactive protein (CRP) concentrations were studied in 17 patients with NSAIDs-induced acute urticaria/angioedema (NSAIDsAU) and in 20 healthy controls. Eleven patients who used NSAIDs were presented at the emergency room with acute urticaria/angioedema while the remaining six manifested the symptoms during the aspirin challenge test. Patients were examined in a dynamic manner: during the acute phase, and next, after subsidence of the symptoms. RESULTS: CRP and IL-6 concentrations increased significantly in patients with NSAIDsAU as compared with their asymptomatic period and the healthy subjects. In addition, NSAIDsAU patients showed elevated concentration of the biomarkers following aspirin provocation with the baseline values recovered in the asymptomatic period. CONCLUSION: These results indicate that an acute systemic inflammatory response is activated in patients with NSAIDs-induced urticaria and/or angioedema. The study supports the evidence proving that up-regulation of CRP and IL-6 in urticaria/angioedema does not necessarily reflect any concomitant infection or other inflammatory processes, but may be due to the disease itself.
Assuntos
Angioedema/induzido quimicamente , Anti-Inflamatórios não Esteroides/antagonistas & inibidores , Aspirina/administração & dosagem , Biomarcadores/sangue , Inflamação/diagnóstico , Urticária/induzido quimicamente , Adolescente , Adulto , Angioedema/sangue , Proteína C-Reativa/análise , Feminino , Humanos , Interleucina-6/sangue , Masculino , Pessoa de Meia-Idade , Urticária/sangue , Adulto JovemRESUMO
The study aimed at evaluating changes in plasma levels of insulin-like growth factor 1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3), testosterone, growth hormone (GH), cortisol, and insulin in resistance-trained male athletes with (n=9) and without (n=9) left ventricular hypertrophy (LVH) in response to eccentric (ECC) and concentric (CON) arm exercise. 10 age-matched healthy non-trained subjects served as controls. M-mode and 2D Doppler echocardiography were used to estimate LV mass.Resting IGF-1 concentration was higher in LVH athletes compared to controls (52 ± 5 nM vs. 46 ± 7 nM, p<0.05). ECC exercise resulted in higher (p<0.05) serum IGF-1 concentrations in athletes with LVH (70 ± 11 nM, n=9) compared to those without LVH (62 ± 10 nM, n=9), and to untrained controls (54 ± 6 nM). Both CON and ECC exercise resulted in higher serum IGFBP-3 levels in LVH athletes compared to controls (242 ± 57 and 274 ± 58, athletes, vs. 215 ± 63 and 244 ± 67, controls, nM, p<0.05). In ECC exercise, GH concentrations were lower in LVH than in non-LVH athletes (4.7 ± 2.1 vs. 6.1 ± 1.8 ng mL(-1), p<0.05). No differences in other hormones were found between groups. In conclusion, LVH is accompanied by elevated resting serum IGF-1 and enhanced response to eccentric arm exercise. These findings suggest a role of IGF-1, possibly released from contracting muscle, in stimulating LV hypertrophy in resistance training.
Assuntos
Braço/fisiologia , Atletas , Hipertrofia Ventricular Esquerda/sangue , Fator de Crescimento Insulin-Like I/metabolismo , Contração Muscular/fisiologia , Treinamento Resistido , Biomarcadores/sangue , Estudos de Casos e Controles , Teste de Esforço , Hormônio do Crescimento Humano/sangue , Humanos , Hidrocortisona/sangue , Hipertrofia Ventricular Esquerda/diagnóstico por imagem , Hipertrofia Ventricular Esquerda/etiologia , Insulina/sangue , Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina/sangue , Masculino , Testosterona/sangue , Ultrassonografia , Adulto JovemRESUMO
BACKGROUND: Overproduction of vascular endothelial growth factor (VEGF) in atopic dermatitis (AD) lesions has previously been observed. It is also known that platelet is an important source of VEGF and platelet factor 4 (PF-4), a potential marker of AD severity. AIM: To evaluate concentrations of VEGF and its soluble receptors (sVEGF-R1 and sVEGF-R2) in the plasma of AD patients and to examine its possible correlation with disease severity and plasma concentrations of PF-4, a platelet activation marker. METHODS: Plasma concentrations of VEGF and its receptors and levels of PF-4 were measured by an immunoenzymatic assay in 51 AD patients and in 35 healthy non-atopic controls. The severity of the disease was evaluated using the eczema area and severity index. RESULTS: AD patients showed significantly increased VEGF and PF-4 plasma concentrations as compared with the controls. Plasma concentrations of sVEGF-R1 and sVEGF-R2 did not differ between the groups. There were no remarkable correlations between plasma VEGF concentration and disease severity or between VEGF and PF-4 concentration. CONCLUSIONS: This study shows that plasma concentration of VEGF may be increased in patients suffering from AD. It seems that plasma VEGF concentration is not a useful marker of disease severity and, apart from platelets, other cells might also release the cytokine.
Assuntos
Dermatite Atópica/sangue , Fator A de Crescimento do Endotélio Vascular/sangue , Adolescente , Adulto , Dermatite Atópica/diagnóstico , Feminino , Humanos , Masculino , Ativação Plaquetária , Fator Plaquetário 4/sangue , Índice de Gravidade de Doença , Testes Cutâneos , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/sangue , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/sangue , Adulto JovemRESUMO
Dc-pulsed magnetron sputtering from Ti target in reactive Ar+O2+N2 atmosphere was used to grow stoichiometric TiO2:N and non-stoichiometric TiO2-x:N thin films. X-ray diffraction at glancing incidence, atomic force microscopy AFM, scanning electron microscopy SEM, X-ray photoelectron spectroscopy XPS, and optical spectrophotometry were applied for sample characterization. Measurements of photocurrent versus voltage and wavelength over the ultraviolet uv and visible vis ranges of the light spectrum were performed in order to assess the performance of nitrogen-doped titanium dioxide thin films as photoanodes for hydrogen generation in photoelectrochemical cells, PEC. Undoped TiO2 and TiO2-x films were found to be composed of anatase and rutile mixture with larger anatase crystallites (25-35 nm) while the growth of smaller rutile crystallites (6-10 nm) predominated at higher nitrogen flow rates etaN2 as measured in standard cubic centimeters, sccm. Nitrogen-to-titanium ratio increased from N/Ti = 0.05 at etaN2 = 0.8 sccm for stoichiometric TiO2:N to N/Ti = 0.11 at etaN2 = 0.8 sccm for nonstoichiometric TiO2-x:N thin films. A red-shift in the optical absorbance was observed with an increase in etaN2. Doping with nitrogen improved photoelectrochemical properties over the visible range of the light spectrum in the case of nonstoichiometric samples.
Assuntos
Cristalização/métodos , Membranas Artificiais , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Nitrogênio/química , Titânio/química , Condutividade Elétrica , Eletroquímica/métodos , Teste de Materiais , Tamanho da Partícula , Fotoquímica/métodosRESUMO
The patterns of acute-phase response (APR) biomarkers differ upon various inflammatory conditions. Little information is available on the systemic inflammatory response in urticaria/angio-oedema. It has been shown that concentrations of circulating APR biomarkers, IL-6 and C-reactive protein (CRP), are elevated more in severe chronic urticaria (CU) than in patients showing milder urticarial symptoms. It is not clear whether the increase of IL-6 and CRP is merely an epiphenomenon or may contribute to the pathogenesis of CU. It is tempting to speculate that mediators of APR may enhance urticarial inflammation. In addition, there is some association between APR and activation of coagulation/fibrinolysis in CU. It is well known that even slight elevation in CRP baseline concentration is enough to produce significant increase in cardiovascular risk. In this light, one should ask whether CU patients, in particular those showing stronger systemic inflammatory response and long-lasting course are more vulnerable to the cardiovascular events. Apart from highly troublesome symptoms and low quality of life, CU may then involve some remote, serious systemic consequences. Taken together, CU can be identified as a mast cell- and basophil-dependent inflammatory disorder of the skin, which is accompanied by APR. Characterization of APR in CU may appear essential for an insight into the activity of this disease and for assessment of the inflammation degree. Moreover, measurement of these biomarkers might be particularly relevant while assessing CU patients demanding an anti-inflammatory or immunosuppressive therapy. This review summarizes information regarding APR in the course of urticaria/angio-oedema.
Assuntos
Reação de Fase Aguda , Urticária/fisiopatologia , Coagulação Sanguínea , Proteína C-Reativa/metabolismo , Doença Crônica , Sulfato de Desidroepiandrosterona/sangue , Fibrinólise , Humanos , Interleucina-6/sangue , Metaloproteinase 9 da Matriz/metabolismo , Urticária/metabolismoRESUMO
Delayed pressure urticaria (DPU) is characterized by swelling in the area of sustained pressure on the skin. The reported case was a potentially life-threatening complication due to mucosal edema following esophagogastroduodenoscopy (EGD). A 37-year-old man, suffering from severe DPU and chronic spontaneous urticaria, had undergone EGD due to dyspeptic symptoms. A few hours after the EGD procedure, the patient showed both dysphagia and dyspnea. A physical examination indicated massive tongue base and pharynx edema. We suggest that these symptoms were most likely due to the pressure exerted by EGD. No other apparent origins such as angioedema or late-phase allergic reaction to drugs were identified. One should be aware of the increased risk of developing airway and gastrointestinal obstruction during medical procedures associated with compression, such as EGD or endotracheal intubation, in DPU patients.
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Transtornos de Deglutição/etiologia , Dispneia/etiologia , Edema/etiologia , Endoscopia do Sistema Digestório/efeitos adversos , Pressão/efeitos adversos , Doenças da Língua/etiologia , Urticária/complicações , Adulto , Doença Crônica , Edema/diagnóstico , Humanos , Masculino , Doenças da Língua/diagnósticoRESUMO
BACKGROUND: Our previous study was the first to demonstrate enhanced plasma IL-6 concentrations in chronic urticaria (CU). It is known that C-reactive protein (CRP) is a sensitive marker of an underlying systemic inflammation, triggered mainly as a response to IL-6. OBJECTIVE: To evaluate plasma IL-6 concentration in CU patients relating to the clinical disease activity and serum CRP concentration. METHODS: Serum CRP and plasma IL-6 concentrations were measured in 58 CU patients and 30 healthy subjects. Ten CU patients were evaluated twice, during the active period as well as upon the spontaneous clinical remission of the disease. CU activity was assessed with the use of the symptom scores recommended by EAACI/GALEN/EDF guidelines. RESULTS: IL-6 and CRP concentrations were significantly increased in CU patients as compared with the healthy subjects, whereas they decreased remarkably upon the spontaneous remission. IL-6 concentration was associated with weekly urticaria activity scores and also significant differences were found between patients showing different degrees of urticarial activity. Significant correlation was observed between IL-6 and CRP concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: This study reinforces evidence that, apart from a local cutaneous inflammation, CU is associated with a systemic inflammatory response. Such acute-phase response is manifested by increased circulating IL-6, which varies along with CRP changes and may be related to the urticarial activity.
Assuntos
Proteína C-Reativa/metabolismo , Interleucina-6/sangue , Urticária/sangue , Urticária/fisiopatologia , Reação de Fase Aguda , Adulto , Biomarcadores/sangue , Doença Crônica , Feminino , Humanos , Inflamação/sangue , Masculino , Índice de Gravidade de DoençaRESUMO
BACKGROUND: Activation of fibrinolysis system has been observed in spontaneous urticaria. AIM: To investigate the haemostatic activation in DPU, expressed by plasma D-dimer concentration, the index of active fibrinolysis. METHODS: Plasma D-dimer concentrations were measured in eight patients with pure DPU of varying severity as well as in 30 healthy subjects. RESULTS: The analysis of individual results indicated that three patients of the eight show d-dimer concentration higher than the upper limit of the normal range. All the patients had severe DPU and manifested extensive lesions. The remaining five milder cases showed values within the normal range. CONCLUSIONS: Our analysis revealed that DPU patients may differ in their haemostatic activation state depending on severity of the disease. In severe DPU cases hyperfibrinolysis may occur, manifested by elevated plasma d-dimer concentration, probably as a consequence of the systemic inflammatory response.