RESUMO
OBJECTIVE: To prepare resveratrol solid lipid nanoparticles (Res-SLN) and investigate its physical and chemical speciality and anticancer effects in vitro. METHODS: Res-SLN was prepared by the solvent emulsification-evaporation method. Its morphology, particle size and zata potential were examined by transmission electron microscope and laser granularity equipment. Its entrapment efficiency, drug loading, release concentration were determined by HPLC. Its anticancer effect of Res-SLN in vitro were studied by MTT. RESULTS: Res-SLN assumed spherical shape. Its distribution of diameter was even with average particle size of 96. 7 nm, zata potential was--16.3mV, drug loading was (7.95 +/- 0.21)%, entrapment efficiency was (91.34 +/- 0.18)%; Res-SLN could retard drug release in vitro and its cytotoxicity was significantly higher than that of oridonin solution against HepG2 cells. CONCLUSION: Res-SLN has high entrapment efficiency and drug loading, uniform particle size, and can retard drug release in vitro and enhance anticancer effect.
Assuntos
Antineoplásicos Fitogênicos/farmacocinética , Portadores de Fármacos/química , Lipídeos/química , Nanopartículas/química , Estilbenos/administração & dosagem , Estilbenos/farmacocinética , Antineoplásicos Fitogênicos/administração & dosagem , Antineoplásicos Fitogênicos/química , Proliferação de Células/efeitos dos fármacos , Preparações de Ação Retardada , Composição de Medicamentos/métodos , Estabilidade de Medicamentos , Glicerídeos/química , Células Hep G2 , Humanos , Nanopartículas/ultraestrutura , Tamanho da Partícula , Fosfolipídeos/química , Poloxâmero/química , Resveratrol , Estilbenos/química , Propriedades de SuperfícieRESUMO
OBJECTIVE: To study the preparation of alpha-Hydrocycholic-beta-Cyclodextrin inclusion compound. METHODS: It was studied with orthogonal design to analysis three factors of inclusion rate such as the weight ratio between HDCA and beta-Cyclodextrin, the temperature and the reaction time. Fourier transform infrared spectroscopy, scanning electron microscopy, taste and solubility test were used to identify the inclusion compound. RESULTS: Stable inclusion compound was made by HDCA and beta-Cyclodextrin. The weight ratio between HDCA and beta-Cyclodextrin was the most important factor. The alpha-Hydrocycholic-beta-Cyclodextrin inclusion compound taste and the solubility were improved. CONCLUSION: The optimum preparation condition is alpha-Hydrocycholic : beta-Cyclodextrin = 8.67 : 1, the inclusion temperature is 60 degrees C and the inclusion time is 30 minutes.
Assuntos
Ácido Desoxicólico/química , Medicamentos de Ervas Chinesas/química , Tecnologia Farmacêutica/métodos , beta-Ciclodextrinas/química , Análise de Variância , Portadores de Fármacos , Composição de Medicamentos/métodos , Medicamentos de Ervas Chinesas/análise , Plantas Medicinais/química , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Fatores de TempoRESUMO
Palynology, one science of plant's pollen and spores, has been proven to be a new frontier discipline. Because of the characteristics of pollen and spores, such as small size, light weight, large amount, and difficult to be found, they can leave physical evidence and provide new clues to solve a case. Therefore palynology has a good prospect for practical application in forensic medicine. The paper intends to analyze the advantage and limitation of palynology in forensic medicine by reviewing its general characteristics, classification, morphology, and disseminating circadian rhythm. We hope to provide some reference to apply palynology in forensic medicine.