Self-enhanced peroxidase-like activity in a wide pH range enabled by heterostructured Au/MOF nanozymes for multiple ascorbic acid-related bioenzyme analyses.

Nanozymes, a class of catalytic nanomaterials, have shown great potential to substitute natural enzymes in various applications. Nevertheless, the pursuit of high-efficiency peroxidase-like activity in a wide pH range is one of the major challenges existing in designing nanozymes. A feasible strategy is to construct an artificial active center by using porous materials as stable supporting structures, which can actively modulate biocatalytic activities via their porous atomic structures and more active sites. Herein, a gold nanoparticles/metal-organic framework (MOF) heterostructure was prepared using UiO-66 as a stable support structure (Au NPs/UiO-66), which demonstrates enhanced peroxidase-like activity, ∼8.95 times higher than that of pure Au NPs. Strikingly, Au NPs/UiO-66 exhibits excellent stability (maintains above 80% activity at 40-70 °C and retains 93% activity after 3 months of storage) and sustained high relative activity (above 90%) over a pH range of 5.0-9.0 due to the homogeneous dispersibility of free-ligand Au NPs and the strong chemical interaction between the Au NPs and the UiO-66 host. Moreover, a colorimetric assay of ascorbic acid (AA) and three AA-related biological enzymes was developed based on Au NPs/UiO-66 nanozyme, which has a good linear detection range and excellent anti-interference ability. This work provides important guidance for the expansion of metal NPs/MOF heterostructure nanozymes and their application prospects in the development of biosensors.

[To evaluate the efficacy of Yishen Tongluo decoction combined with low-dose tadalafil in the treatment of diabetic erectile dysfunction with kidney deficiency and blood stasis syndrome].

OBJECTIVE: To observe the clinical efficacy of the combined treatment of Yishen Tongluo formula and low-dose tadalafil in diabetic erectile dysfunction. METHODS: A total of 80 patients with diabetic erectile dysfunction were randomly divided into two groups. The control group given tadalafil treatment, observation group in the control group given Yishen Tongluo Formula on the basis of treatment. The treatment period was 8 weeks. Erectile function were observed before and after treatment in the two groups patients-5 international questionnaire (IIEF - 5) score, erection quality scale (EQS) score, erectile hardness (EHS) score, TCM syndrome integral, content of serum homocysteine (HCY), endothelial function index ï¼»serum levels of prostaglandin I2 (PGI2)ï¼½ and endothelin (ET) content, a The changes of nitrogen oxide (NO), glucose and lipid metabolism indexes ï¼»triglyceride (TC), total cholesterol (TG)ï¼½ and oxidative stress related factors ï¼»total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px)ï¼½ were evaluated, and the clinical efficacy of the two groups was evaluated. RESULTS: In terms of overall efficacy rate, the observation group (79.4%) outperformed that of the control (48.7%, P< 0.01).After treatment, the IIEF-5 score, EQS score, EHS score, and serum levels of PGI2, NO, T-AOC and GSH-Px were higher than those before treatment in the two groups (P< 0.05). The TCM syndrome score and serum HCY, ET-1, TC and TG were lower than those before treatment (P< 0.05), and the comparison group's consequence was comparatively worse than the group under observation (P< 0.01). CONCLUSIONS: Yishen Tongluo Formula can dramatically enhance the erectile dysfunction andimprovement of glucose-lipid metabolism when adopted in together with low-dose tadalafil.

Optimization of retina-like illumination patterns in ghost imaging.

Ghost imaging (GI) reconstructs images using a single-pixel or bucket detector, which has the advantages of scattering robustness, wide spectrum, and beyond-visual-field imaging. However, this technique needs large amounts of measurements to obtain a sharp image. Numerous methods are proposed to overcome this disadvantage. Retina-like patterns, as one of the compressive sensing approaches, enhance the imaging quality of the region of interest (ROI) while maintaining measurements. The design of the retina-like patterns determines the performance of the ROI in the reconstructed image. Unlike the conventional method to fill in ROI with random patterns, optimizing retina-like patterns by filling in the ROI with the patterns containing the sparsity prior of objects is proposed. The proposed method is then verified by simulations and experiments compared with conventional GI, retina-like GI, and GI using patterns optimized by principal component analysis. The method using optimized retina-like patterns obtains the best imaging quality in ROI among other methods. Meanwhile, the good generalization capability of the optimized retina-like pattern is also verified. The feature information of the target can be obtained while designing the size and position of the ROI of retina-like patterns to optimize the ROI pattern. The proposed method facilitates the realization of high-quality GI.

High-density Ag nanosheets for selective electrochemical CO2 reduction to CO.

To increase the specific surface area, high-density (i.e. number per unit area) Ag nanosheets (ANS) with large electrochemically active surface area and rich edge active sites over Ag plates were synthesized via a facile electrodeposition approach in a double electrode system at a constant current of -1 mA for 1800 s. By adjusting the concentration of H3BO3 (0.5 M, 0.1 M and 0.05 M), which is used to control the growth direction of ANS, ANS-20, -50, -350 were obtained with varying thickness of 20 nm, 50 nm, and 350 nm, respectively. Notably, ANS-20 showed a remarkable current density of -6.48 mA cm-2 at -0.9 V versus the reversible hydrogen electrode (RHE), which is almost 1.6 and 2.4 times as high as those of ANS-50 and -350, respectively. Furthermore, ANS-20 exhibits the best CO selectivity of 91.2% at -0.8 V versus RHE, while the other two give 84.6% and 77.9% at the same potential. The excellent performance of ANS-20 is attributed to its rich edge active sites and large electrochemically active surface area (ECSA).

Au aerogel for selective CO2electroreduction to CO: ultrafast preparation with high performance.

Noble metal aerogels (NMAs) have been used in a variety of (photo-)electrocatalytic reactions, but pure Au aerogel (AG) has not been used in CO2electroreduction to date. To explore the potential application in this direction, AG was prepared to be used as the cathode in CO2electroreduction to CO. However, the gelation time of NMAs is usually very long, up to several weeks. Here, an excess NaBH4and turbulence mixing-promoted gelation approach was developed by introducing magnetic stirring as an external force field, which therefore greatly shortened the formation time of Au gels to several seconds. The AG-3 (AG with Au loading of 0.003 g) exhibited a high CO Faradaic efficiency (FE) of 95.6% at an extremely low overpotential of 0.39 V, and over 91% of CO FE was reached in a wide window of -0.4 to -0.7 V versus the reversible hydrogen electrode (RHE). Partial current density in CO was measured to be -19.35 mA cm-2at -0.8 V versus RHE under 1 atm of CO2. The excellent performance should be ascribed to its porous structure, abundant active sites, and large electrochemical active surface area. It provides a new method for preparation of AG with ultrafast gelation time and large production at room temperature, and the resulting pure AG was for the first time used in the field of CO2electroreduction.

A Novel Approach of Parallel Retina-Like Computational Ghost Imaging.

Computational ghost imaging (CGI), with the advantages of wide spectrum, low cost, and robustness to light scattering, has been widely used in many applications. The key issue is long time correlations for acceptable imaging quality. To overcome the issue, we propose parallel retina-like computational ghost imaging (PRGI) method to improve the performance of CGI. In the PRGI scheme, sampling and reconstruction are carried out by using the patterns which are divided into blocks from designed retina-like patterns. Then, the reconstructed image of each block is stitched into the entire image corresponding to the object. The simulations demonstrate that the proposed PRGI method can obtain a sharper image while greatly reducing the time cost than CGI based on compressive sensing (CSGI), parallel architecture (PGI), and retina-like structure (RGI), thereby improving the performance of CGI. The proposed method with reasonable structure design and variable selection may lead to improve performance for similar imaging methods and provide a novel technique for real-time imaging applications.

Ultrathin Tunable Lens Based on Boundary Tension Effect.

Solid and liquid lenses are commonly used in optical design. Such lenses have suitable thicknesses due to their working principle and processing mode. Thus, zoom optical systems comprising solid and liquid lenses are extremely large. This work presents a new ultrathin tunable lens (UTL) comprising two liquid film lenses (LFLs) obtained through aspheric deformation and produced from the surface of a micro-liquid under gravity and boundary tension. The UTL can flexibly change focal lengths between positive and negative lenses when the device thickness is merely 2.15 mm. The proposed lens has the advantages of small volume, light weight, simple fabrication, and independence from external force during zooming. This research makes up for the drawback that traditional solid and liquid lenses cannot further reduce their thicknesses. The proposed UTL provides a new lens form and fabrication method, and can be used to replace solid and liquid lenses for designing miniature zoom optical systems.

Combining Non-Uniform Time Slice and Finite Difference to Improve 3D Ghost Imaging.

Three-dimensional ghost imaging (3DGI) using a detector is widely used in many applications. The performance of 3DGI based on a uniform time slice is difficult to improve because obtaining an accurate time-slice position remains a challenge. This paper reports a novel structure based on non-uniform time slice combined with finite difference. In this approach, finite difference is beneficial to improving sensitivity of zero crossing to accurately obtain the position of the target in the field of view. Simultaneously, non-uniform time slice is used to quickly obtain 3DGI on an interesting target. Results show that better performances of 3DGI are obtained by our proposed method compared to the traditional method. Moreover, the relation between time slice and the signal-noise-ratio of 3DGI is discussed, and the optimal differential distance is obtained, thus motivating the development of a high-performance 3DGI.

A Hybrid Bionic Image Sensor Achieving FOV Extension and Foveated Imaging.

Based on bionic compound eye and human foveated imaging mechanisms, a hybrid bionic image sensor (HBIS) is proposed in this paper to extend the field of view (FOV) with high resolution. First, the hybrid bionic imaging model was developed and the structure parameters of the HBIS were deduced. Second, the properties of the HBIS were simulated, including FOV extension, super-resolution imaging, foveal ratio and so on. Third, a prototype of the HBIS was developed to validate the theory. Imaging experiments were carried out, and the results are in accordance with the simulations, proving the potential of the HBIS for large FOV and high-resolution imaging with low cost.

Improving the performance of time-domain pulsed echo laser profile using tunable lens.

We theoretically and experimentally examined the effects of the different positions between receiving lens (RL) and detector, namely, defocus, off-axis, and tilt, on the time-domain pulsed echo laser profile (TDPELP). Results show that distortions including saturation and broadening of TDPELP are obtained, regardless of the position between RL and detector. Thus, we adjust the focal length of RL to successfully obtain an optimal TDPELP using tunable lens under extreme situations, such as too strong or too weak intensity of the TDPELP.

Compound eye and retina-like combination sensor with a large field of view based on a space-variant curved micro lens array.

A compound eye and retina-like combination sensor based on a space-variant curved micro lens array (CMLA) is proposed to simultaneously offer the large FOV characteristic of a compound eye and retina-like feature of a single aperture eye. The mathematical models of the sensor are developed and the structure parameters of the space-variant CMLA are deduced. Modeling verification is carried out and the results show that the whole field of view (FOV) of the sensor is 105° and the optical information loss rate is 0.06 when the sector is 32. Imaging simulations illustrate that the sensor possesses the retina-like property, i.e., logarithmic-polar transformation. Meanwhile, the simulation results indicate that the overlapping angles between the two micro lenses on the adjacent rings can be reduced by decreasing the rings and the blind radius, and increasing the sectors. This work is beneficial for large FOV and time-efficient applications.

Analytical and numerical approaches to study echo laser pulse profile affected by target and atmospheric turbulence.

The integrated model of echo laser pulse profile (ELPP) of a target with arbitrary shape is studied under the condition of the ELPP affected by target and atmospheric turbulence simultaneously. The ELPPs of four typical targets (a plane, a cone, a sphere and an aspherical surface) are employed to test the validity of the model by analytical and numerical approaches. Based on simulations of the ELPP under different targets and atmospheric turbulence intensity, the results show a good agreement between two methods, and the ELPP of a target with discontinuous surface is more easily affected by atmospheric turbulence than that with a continuous surface. Besides that, we study the relationship between the number of grids and the relative error of analytical and numerical approaches, which are of interest to obtain the optimal number of grids used in the simulations.

Modeling and simulations on retina-like sensors based on curved surface.

A space-variant lens array on a curved surface (SVLACS) with a large field of view is proposed to decrease the size and improve the performance of a space-variant lens array on a plane (SVLAP). The whole mathematical models are developed and tested, and comparative simulations between SVLACS and SVLAP are carried out. Under the identical simulated situations, the radius of SVLACS decreases to 58% of SVLAP. Meanwhile, the performance of optical information loss rate is improved from 0.22 to 0.08. The results are beneficial for designing a retina-like image sensor based on SVLACS.

Primary multiple endocrine insufficiency during immune checkpoint inhibitor treatment: A case report.

RATIONALE: Immunotherapy with immune checkpoint inhibitors (ICI) has shown promising activity against many tumor types. However, they can also induce a wide array of immune-related adverse events, ranging from mild to fatal. Primary 3 endocrine gland insufficiency during treatment with ICI has rarely been reported. PATIENT CONCERNS: We report the case of a 33-year-old man with Ewing sarcoma who was treated with toripalimab as a second-line treatment. Approximately 11 months after initiating treatment, the patient developed subclinical hypothyroidism, which was followed by adrenal insufficiency and hypogonadism 6 months later. Consequently, the decision was made to discontinue ICI therapy and initiate hormone replacement therapy to manage endocrine deficiencies. DIAGNOSES: Serum adrenocorticotropic hormone, thyroid stimulating hormone, and prolactin levels increased significantly, while cortisol, estradiol, and testosterone levels decreased (Table 1). The patient had negative findings on the pituitary MRI. INTERVENTION: As part of the management strategy, ICI therapy was ceased and hormone replacement therapy was commenced to address endocrine deficiencies. OUTCOMES: After hormone replacement therapy, his symptoms improved and follow-up examinations showed normalization of hormone levels. LESSONS: Clinicians should be aware of the potential of immune checkpoint inhibitor therapy to cause endocrine dysfunction. Prompt recognition and management of these adverse events are crucial for patient health and quality of life.

Effects of co-supplementation of chromium and magnesium on metabolic profiles, inflammation, and oxidative stress in impaired glucose tolerance.

PURPOSE: To evaluate the effects of chromium (Cr) and magnesium (Mg) ions on metabolic profiles, inflammation, and oxidative stress with impaired glucose tolerance (IGT) and insulin resistance (IR). METHODS: 120 individuals with IGT and IR were randomly divided into four groups treated with (1) chromium, (2) magnesium, (3) chromium and magnesium or (4) placebo. Metabolic and inflammatory indicators were measured at baseline and after 3 months intervention. RESULTS: Comparison among groups showed that fasting plasma glucose (FPG), 2 h post glucose (2hPPG), fasting insulin (FINS) and homeostatic model assessment for insulin resistance (HOMA-IR) in Cr + Mg group were significantly decreased compared with the other three groups (p < .05), and high density lipoprotein (HDL-c) levels were higher. 8-iso prostaglandin F2 alpha (8-iso-PGF2a) decreased in Cr, Mg, and Cr + Mg groups compared with placebo (p < .05), and 8-iso-PGF2a decreased in Cr + Mg groups compared with Cr group and Mg groups (p > .05). Intra-group comparison showed that the levels of FPG, 2hPPG and FINS in Cr + Mg group were significantly decreased after intervention (p < .05), and FINS in Mg group was significantly decreased (p < .01). The levels of HDL-c and triacylglycerol (TG) in Cr + Mg group were significantly improved (p < .05). The level of HDL-c in Mg group was significantly improved compared with baseline (p < .05). Compared with baseline, high-sensitivity C-reactive protein (hsCRP) levels in Cr + Mg group and Mg group were significantly decreased (p < .05). CONCLUSIONS: The co-supplementation of Cr and Mg improves glycemic and lipid levels and reduces the inflammatory response and oxidative stress profiles of individuals with impaired glucose tolerance and insulin resistance.

High-fidelity intracellular imaging of multiple miRNAs via stimulus-responsive nanocarriers and catalytic hairpin assembly.

An advanced nanoplatform was developed by integrating catalytic hairpin assembly (CHA) with glutathione-responsive nanocarriers, enabling superior imaging of dual cancer-related miRNAs. Two distinct CHA circuits for the sensing of miRNA-21 and miRNA-155 were functionalized on biodegraded MnO2. In the presence of GSH and the corresponding miRNAs, the degraded MnO2 released the DNA cargos, activating the CHA circuits and recovering the fluorescence. This approach offers a reliable sensing performance with highly selective cell-identification capacity, positioning it as a pivotal tool for imaging multiple biomarkers in living cells.

Endogenous glutathione-activated DNA nanoprobes for spatially controllable imaging of microRNA in living cells.

A DNA nanoprobe, activated by glutathione (GSH), was designed to enable spatially selective sensing and imaging of miRNA in living cells. The nanoprobe was constructed using nano-sized metal-organic frameworks (MOFs) and DNA hairpin probes tethered to the surface of the MOFs, with the loop portion of the hairpin structure containing a disulfide bond. Cleavage of the disulfide bond by GSH triggers a strand-displacement reaction with target miRNAs, facilitating in situ readout of the fluorescence signal. The synergy of endogenous GSH activation and MOF improves the spatial resolution of miRNA detection and imaging.

DNA-modulated single-atom nanozymes with enhanced enzyme-like activity for ultrasensitive detection of dopamine.

Despite the current progress in optimizing and tailoring the performance of nanozymes through structural and synthetic adaptation, there is still a lack of dynamic modulation approaches to alter their catalytic activity. Here, we demonstrate that DNA can act as an auxiliary regulator via a straightforward incubation method with Fe-N-C single-atom nanozymes (SAzymes), causing a leap in the enzyme-like activity of Fe-N-C from moderate to a higher level. The DNA-assisted enhancement is attributed to the increased substrate affinity of Fe-N-C nanozymes through electrostatic attraction between the substrate and DNA. Based on the prepared DNA/Fe-N-C system, colorimetric sensors for dopamine (DA) detection were constructed. Surprisingly, the incorporation of DNA not only enabled the detection of DA in a low concentration range, but also greatly improved the sensitivity with a 436-fold decrease in detection limit. The quantitative determination of DA was achieved in two-segment linear ranges of 0.01-4 µM and 5-100 µM with an ultralow detection limit of 9.56 nM. The DNA/Fe-N-C system shows superior performance compared to the original Fe-N-C system, making it an ideal choice for nanozyme-based biosensors. This simple design approach has paved the way for enhancing nanozyme activity and is expected to serve as a general strategy for optimizing biosensor performance.

Affibody Modified G-quadruplex DNA Micelles Incorporating Polymeric 5-Fluorodeoxyuridine for Targeted Delivery of Curcumin to Enhance Synergetic Therapy of HER2 Positive Gastric Cancer.

Combination chemotherapy is emerging as an important strategy for cancer treatment with decreased side effects. However, chemotherapeutic drugs with different solubility are not easy to realize co-delivery in traditional nanocarriers. Herein, an affibody modified G-quadruplex DNA micellar prodrug (affi-F/GQs) of hydrophilic 5-fluorodeoxyuridine (FUdR) by integrating polymeric FUdRs into DNA strands is developed for the first time. To achieve synergistic efficacy with hydrophobic drugs, curcumin (Cur) is co-loaded into affi-F/GQs micelles to prepare the dual drug-loaded DNA micelles (Cur@affi-F/GQs), in which affibody is employed as a targeting moiety to facilitate HER2 receptor-mediated uptake. Cur@affi-F/GQs have a small size of approximately 130 nm and exhibit excellent stability. The system co-delivers FUdR and Cur in a ratiometric manner, and the drug loading rates are 21.1% and 5.6%, respectively. Compared with the physical combination of FUdR and Cur, Cur@affi-F/GQs show higher cytotoxicity and greater synergistic effect on HER2 positive gastric cancer N87 cells. Surprisingly, Cur@affi-F/GQs significantly enhance the expression and activity of apoptosis-associated proteins in Bcl-2/Bax-caspase 8, 9-caspase 3 apoptotic pathway, which is the main factor in the death of tumor cells induced by FUdR. Overall, this nanoencapsulation is a promising candidate for the targeted co-delivery of drugs with significant differences in solubility.

Amphiphilic Cationic Peptide-Coated PHA Nanosphere as an Efficient Vector for Multiple-Drug Delivery.

Amphiphilic core-shell (ACS) nanoparticles are gaining increasing research interest for multi-drug delivery in cancer therapy. In this work, a new cationic peptide-coated PHA nanosphere was prepared by self-assembly of a hydrophobic core of biodegradable poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and a hydrophilic shell of fusion proteins of PHA granule-associated protein (PhaP) and cationic peptide RALA through a strong hydrophobic effect. The hydrophobic drug curcumin (Cur) was encapsulated in PHBHHx nanoparticles. The chemotherapy drug 5-fluorouracil (5-FU) was administered in the form of its metabolite oligomeric 5-fluorodeoxyuridine (FUdR). Fifteen consecutive FUdR (FUdR15S) were adsorbed on the surface of PHBHHx nanoparticles by electrostatic interaction with RALA to form Cur@PHBX-PR/FUdR15S. Such amphiphilic cationic nanospheres had 88.3% EE of Cur and the drug loading of Cur and FUdR were 7.8% and 12.1%. The dual-drug-loaded nanospheres showed a time-differential release of Cur and FUdR. In addition, Cur@PHBX-PR/FUdR15S exhibited excellent anticancer activity and played a vital role in promoting the synergistic effect of FUdR and Cur in gastric cancer cells. The exploration of antitumor mechanisms demonstrated that Cur improved the activity of apoptosis-related proteins and cancer cells sensitized to FUdR. This amphiphilic core-shell system can serve as a general platform for sequential delivery of multiple drugs to treat several cancer cells.