Omentin-1: Protective impact on ischemic stroke via ameliorating atherosclerosis.

Omentin-1, a newly identified adipokine, has recently been revealed as a novel biomarker for ischemic stroke (IS). Low circulating omentin-1 levels could indicate a high risk of IS, and elevated omentin-1 levels exert a favorable impact on cerebral ischemia. Furthermore, omentin-1 has anti-atherosclerotic, anti-inflammatory, and cardiovascular protective capabilities through the intracellular Akt/AMP-activated protein kinase (AMPK)/ nuclear factor-κB (NF-κB) and certain protein kinase (ERK, JNK, and p38) signaling pathways. Omentin-1 also alleviates endothelial cell dysfunction, improves revascularization via the Akt-endothelial nitric-oxide synthase (eNOS) regulatory axis, promotes endothelium-dependent vasodilation through endothelium-derived NO in an eNOS fashion, and inhibits VSMC proliferation by means of AMPK/ERK signaling pathways, VSMC migration via inactivation of the NADPH oxidase (NOX)/ROS/p38/HSP27 pathways and artery calcification via the PI3K-Akt pathway. These findings indicate that omentin-1 may be a negative mediator of IS. Pharmacologically, several lines of clinical evidence indicate that metformin and statins could elevate omentin-1 levels, although the specific mechanism has not been precisely delineated until now. This study is the first to summarize the comprehensive mechanisms between omentin-1 and atherosclerosis and to review the shielding effect of omentin-1 on IS. We shed light on omentin-1 as a novel therapeutic target for combating IS.

Structure of the human sodium leak channel NALCN in complex with FAM155A.

NALCN, a sodium leak channel expressed mainly in the central nervous system, is responsible for the resting Na+ permeability that controls neuronal excitability. Dysfunctions of the NALCN channelosome, NALCN with several auxiliary subunits, are associated with a variety of human diseases. Here, we report the cryo-EM structure of human NALCN in complex with FAM155A at an overall resolution of 3.1 angstroms. FAM155A forms extensive interactions with the extracellular loops of NALCN that may help stabilize NALCN in the membrane. A Na+ ion-binding site, reminiscent of a Ca2+ binding site in Cav channels, is identified in the unique EEKE selectivity filter. Despite its 'leaky' nature, the channel is closed and the intracellular gate is sealed by S6I, II-III linker and III-IV linker. Our study establishes the molecular basis of Na+ permeation and voltage sensitivity, and provides important clues to the mechanistic understanding of NALCN regulation and NALCN channelosome-related diseases.

Ionic-liquid pretreatment of cassava residues for the cogeneration of fermentative hydrogen and methane.

An ionic liquid of N-methylmorpholine-N-oxide (NMMO) was used to effectively pretreat cassava residues for the efficient enzymatical hydrolysis and cogeneration of fermentative hydrogen and methane. The reducing sugar yield of enzymolysed cassava residues with NMMO pretreatment improved from 36 to 42g/100g cassava residues. Scanning electron microscopy images revealed the formation of deep grooves (∼4µm wide) and numerous pores in the cassava residues pretreated with NMMO. X-ray diffraction patterns showed that the crystallinity coefficient of NMMO-pretreated cassava residues decreased from 40 to 34. Fourier transform infrared spectra indicated that crystal cellulose I was partially transformed to amorphous cellulose II in the NMMO-pretreated cassava residues. This transformation resulted in a reduced crystallinity index from 0.85 to 0.77. Hydrogen yield from the enzymolysed cassava residues pretreated with NMMO increased from 92.3 to 126mL/gTVS, and the sequential methane yield correspondingly increased from 79.4 to 101.6mL/g TVS.

Boosting biomethane yield and production rate with graphene: The potential of direct interspecies electron transfer in anaerobic digestion.

Interspecies electron transfer between bacteria and archaea plays a vital role in enhancing energy efficiency of anaerobic digestion (AD). Conductive carbon materials (i.e. graphene nanomaterial and activated charcoal) were assessed to enhance AD of ethanol (a key intermediate product after acidogenesis of algae). The addition of graphene (1.0g/L) resulted in the highest biomethane yield (695.0±9.1mL/g) and production rate (95.7±7.6mL/g/d), corresponding to an enhancement of 25.0% in biomethane yield and 19.5% in production rate. The ethanol degradation constant was accordingly improved by 29.1% in the presence of graphene. Microbial analyses revealed that electrogenic bacteria of Geobacter and Pseudomonas along with archaea Methanobacterium and Methanospirillum might participate in direct interspecies electron transfer (DIET). Theoretical calculations provided evidence that graphene-based DIET can sustained a much higher electron transfer flux than conventional hydrogen transfer.

Enhanced energy recovery from cassava ethanol wastewater through sequential dark hydrogen, photo hydrogen and methane fermentation combined with ammonium removal.

Cassava ethanol wastewater (CEW) was subjected to sequential dark H2, photo H2 and CH4 fermentation to maximize H2 production and energy yield. A relatively low H2 yield of 23.6mL/g soluble chemical oxygen demand (CODs) was obtained in dark fermentation. To eliminate the inhibition of excessive NH4(+) on sequential photo fermentation, zeolite was used to remove NH4(+) in residual dark solution (86.5% removal efficiency). The treated solution from 5gCODs/L of CEW achieved the highest photo H2 yield of 369.7mL/gCODs, while the solution from 20gCODs/L gave the lowest yield of 259.6mL/gCODs. This can be explained that photo H2 yield was correlated to soluble metabolic products (SMPs) yield in dark fermentation, and specific SMPs yield decreased from 38.0 to 18.1mM/g CODs. The total energy yield significantly increased to 8.39kJ/gCODs by combining methanogenesis with a CH4 yield of 117.9mL/gCODs.

Effective instructional procedures for teaching individuals with severe disabilities in motor skills.

The purpose was to describe effective instructional procedures used to teach motor skills to individuals with severe disabilities. Five effective instructional procedures supported with experimental data in adapted physical education are identified through the review of literature: (a) response shaping, (b) increasing prompt hierarchy, (c) decreasing prompt hierarchy, (d) constant time delay, and (e) progressive time delay. The characteristics, documentations, guidelines, and recommendations for application of these procedures are described.

Use of an ecologically based program for teaching motor skills in a naturalistic setting to individuals with disabilities.

The purpose of this action study was to apply an ecological program for teaching motor skills to individuals with disabilities in naturalistic settings. The program was developed based on an ecological survey. Participants were 22 individuals with disabilities, ages 7 to 26 years in the Fall of 1998 and 18 individuals with disabilities, ages 7 to 24 in the Winter of 1999. In each semester, a participant received one or two 50-min, training session(s) per week for 10 weeks. The number of steps in a motor-activity task performed correctly by a participant was measured in the first and last sessions in each semester. The magnitude of increase in the motor performance by participants and answers to questions in a short survey by participants' parents were used to describe the group performance. Analysis showed group performance of targeted motor skills could be improved using this ecological program in this local learning lab for special physical education.

Validity and reliability of the Wood Motor Success Screening Tool in a special physical education learning laboratory.

The purpose of this study was to estimate the concurrent validity and test-retest reliability of the Wood Motor Success Screening Tool. Participants were 66 children with learning and behavioral disabilities (44 boys and 22 girls; M of age= 7.6 yr.; SD of age= 1.8 yr.). The concurrent validity was checked using the Bruininks-Oseretsky Test of Motor Proficiency-Short Form as the criterion measure. A Pearson product-moment correlation of .84 (p< .01), a sensitivity index of .88, and a specificity index of .64 were found for estimating the concurrent validity. The test-retest reliability was checked using scores on the Wood Motor Success Screening Tool administered twice within a week. An intraclass correlation coefficient of .93 from the one-way analysis of variance was found for estimating the test-retest reliability. These results indicated that this test is valid and reliable for assessing young children.