Climate warming could free cold-adapted trees from C-conservative allocation strategy of storage over growth.

Carbon allocation has been fundamental for long-lived trees to survive cold stress at their upper elevation range limit. Although carbon allocation between non-structural carbohydrate (NSC) storage and structural growth is well-documented, it still remains unclear how ongoing climate warming influences these processes, particularly whether these two processes will shift in parallel or respond divergently to warming. Using a combination of an in situ downward-transplant warming experiment and an ex situ chamber warming treatment, we investigated how subalpine fir trees at their upper elevation limit coordinated carbon allocation priority among different sinks (e.g., NSC storage and structural growth) at whole-tree level in response to elevated temperature. We found that transplanted individuals from the upper elevation limit to lower elevations generally induced an increase in specific leaf area, but there was no detected evidence of warming effect on leaf-level saturated photosynthetic rates. Additionally, our results challenged the expectation that climate warming will accelerate structural carbon accumulation while maintaining NSC constant. Instead, individuals favored allocating available carbon to NSC storage over structural growth after 1 year of warming, despite the amplification in total biomass encouraged by both in situ and ex situ experimental warming. Unexpectedly, continued warming drove a regime shift in carbon allocation priority, which was manifested in the increase of NSC storage in synchrony to structural growth enhancement. These findings imply that climate warming would release trees at their cold edge from C-conservative allocation strategy of storage over structural growth. Thus, understanding the strategical regulation of the carbon allocation priority and the distinctive function of carbon sink components is of great implication for predicting tree fate in the future climate warming.

Multiomics analyses reveal the mechanisms of the responses of subalpine treeline trees to phenology and winter low-temperature stress.

Withstanding extreme cold stress is a prerequisite for alpine treeline trees to persist and survive. However, the underlying mechanism by which treeline trees sense phenological changes and survive hard winters has not been fully elucidated. Here, we investigated the physiology, transcriptome, and metabolome of the subalpine treeline species Larix chinensis to identify the molecular mechanism of phenological and cold resistance. Calcium and antioxidant enzyme activities (e.g., superoxide dismutase and glutathione peroxidase) are essential for coping with winter cold stress in L. chinensis. Transcriptome analysis revealed that circadian rhythm and phytohormone signalling transduction played important roles in regulating L. chinensis phenological changes and cold stress responses. The variations in the transcriptome identified were accompanied by the specific accumulation of flavones, flavonols, and monosaccharides. The flavonoid biosynthesis and phenylpropanoid biosynthesis pathways played important roles in the adaptation of L. chinensis to the extreme winter environment, and flavone and flavonol biosynthesis was an important pathway involved in bud burst. In addition, temperature and photoperiod had synergistic influences on the formation and release of bud dormancy. Thus, our findings provided new insights into the mechanism of subalpine treeline formation.

Asymmetric effects of daytime and nighttime warming on alpine treeline recruitment.

Trees at their upper range limits are highly sensitive to climate change, and thus alpine treelines worldwide have changed their recruitment patterns in response to climate warming. However, previous studies focused only on daily mean temperature, neglecting the asymmetric influences of daytime and nighttime warming on recruitments in alpine treelines. Here, based on the compiled dataset of tree recruitment series from 172 alpine treelines across the Northern Hemisphere, we quantified and compared the different effects of daytime and nighttime warming on treeline recruitment using four indices of temperature sensitivity, and assessed the responses of treeline recruitment to warming-induced drought stress. Our analyses demonstrated that even in different environmental regions, both daytime and nighttime warming could significantly promote treeline recruitment, and however, treeline recruitment was much more sensitive to nighttime warming than to daytime warming, which could be attributable to the presence of drought stress. The increasing drought stress primarily driven by daytime warming rather than by nighttime warming would likely constrain the responses of treeline recruitment to daytime warming. Our findings provided compelling evidence that nighttime warming rather than daytime warming could play a primary role in promoting the recruitment in alpine treelines, which was related to the daytime warming-induced drought stress. Thus, daytime and nighttime warming should be considered separately to improve future projections of global change impacts across alpine ecosystems.

Global analysis of the perturbation effects of metal-based nanoparticles on soil nitrogen cycling.

Although studies have investigated the effects of metal-based nanoparticles (MNPs) on soil biogeochemical processes, the results obtained thus far are highly variable. Moreover, we do not yet understand how the impact of MNPs is affected by experimental design and environmental conditions. Herein, we conducted a global analysis to synthesize the effects of MNPs on 17 variables associated with soil nitrogen (N) cycling from 62 studies. Our results showed that MNPs generally exerted inhibitory effects on N-cycling process rates, N-related enzyme activities, and microbial variables. The response of soil N cycling varied with MNP type, and exposure dose was the most decisive factor for the variations in the responses of N-cycling process rates and enzyme activities. Notably, Ag/Ag2 S and CuO had dose-dependent inhibitory effects on ammonia oxidation rates, while CuO and Zn/ZnO showed hormetic effects on nitrification and denitrification rates, respectively. Other experimental design factors (e.g., MNP size and exposure duration) also regulated the effect of MNPs on soil N cycling, and specific MNPs, such as Ag/Ag2 S, exerted stronger effects during long-term (>28 days) exposure. Environmental conditions, including soil pH, organic carbon, texture, and presence/absence of plants, significantly influenced MNP toxicity. For instance, the effects of Ag/Ag2 S on the ammonia oxidation rate and the activity of leucine aminopeptidase were more potent in acid (pH <6), organic matter-limited (organic carbon content ≤10 g kg-1 ), and coarser soils. Overall, these results provide new insights into the general mechanisms by which MNPs alter soil N processes in different environments and underscore the urgent need to perform multivariate and long-term in situ trials in simulated natural environments.

Nanoplastics alter ecosystem multifunctionality and may increase global warming potential.

Although the presence of nanoplastics in aquatic and terrestrial ecosystems has received increasing attention, little is known about its potential effect on ecosystem processes and functions. Here, we evaluated if differentially charged polystyrene (PS) nanoplastics (PS-NH2 and PS-SO3 H) exhibit distinct influences on microbial community structure, nitrogen removal processes (denitrification and anammox), emissions of greenhouse gases (CO2 , CH4 , and N2 O), and ecosystem multifunctionality in soils with and without earthworms through a 42-day microcosm experiment. Our results indicated that nanoplastics significantly altered soil microbial community structure and potential functions, with more pronounced effects for positively charged PS-NH2 than for negatively charged PS-SO3 H. Ecologically relevant concentration (3 g kg-1 ) of nanoplastics inhibited both soil denitrification and anammox rates, while environmentally realistic concentration (0.3 g kg-1 ) of nanoplastics decreased the denitrification rate and enhanced the anammox rate. The soil N2 O flux was always inhibited 6%-51% by both types of nanoplastics, whereas emissions of CO2 and CH4 were enhanced by nanoplastics in most cases. Significantly, although N2 O emissions were decreased by nanoplastics, the global warming potential of total greenhouse gases was increased 21%-75% by nanoplastics in soils without earthworms. Moreover, ecosystem multifunctionality was increased 4%-12% by 0.3 g kg-1 of nanoplastics but decreased 4%-11% by 3 g kg-1 of nanoplastics. Our findings provide the only evidence to date that the rapid increase in nanoplastics is altering not only ecosystem structure and processes but also ecosystem multifunctionality, and it may increase the emission of CO2 and CH4 and their global warming potential to some extent.

Divergent effects of warming on nonstructural carbohydrates in woody plants: a meta-analysis.

Nonstructural carbohydrates (NSC, including soluble sugars and starch) are essential for supporting growth and survival of woody plants, and play multifunctional roles in various ecophysiological processes that are being rapidly changed by climate warming. However, it still remains unclear whether there is a consistent response pattern of NSC dynamics in woody plants to climate warming across organ types and species taxa. Here, based on a compiled database of 52 woody plant species worldwide, we conducted a meta-analysis to investigate the effects of experimental warming on NSC dynamics. Our results indicated that the responses of NSC dynamics to warming were primarily driven by the fluctuations of starch, while soluble sugars did not undergo significant changes. The effects of warming on NSC shifted from negative to positive with the extension of warming duration, while the negative warming effects on NSC became more pronounced as warming magnitude increased. Overall, our study showed the divergent responses of NSC and its components in different organs of woody plants to experimental warming, suggesting a potentially changed carbon (C) balance in woody plants in future global warming. Thus, our findings highlight that predicting future changes in plant functions and terrestrial C cycle requires a mechanism understanding of how NSC is linked to a specific global change driver.

Open riparian canopy and nutrient pollution interactively decrease trophic redundancy and allochthonous resource in streams.

Riparian deforestation, which leads to increase in light intensity and excessive nutrient loading in waterways, are two pervasive environmental stressors in the stream ecosystems. Both have been found to alter basal resource availability and consequently stream food webs. However, their interactive effects on trophic structure in stream food webs are unclear. Here, we manipulated light intensity and nutrient availability in three headwater streams to evaluate their effects on consumer diet composition and food web characteristics (i.e., trophic diversity and redundancy) with stable isotope analysis. Dietary analysis revealed that the relative contribution of stream periphyton to the diets of macroinvertebrates increased, while that of allochthonous resources, specifically leaf litter from the terrestrial ecosystems in the catchment, decreased in response to open canopy and nutrient enrichment in the streams. The trophic diversity also increased with the elevated light intensity and nutrient availability, while the trophic redundancy decreased, suggesting a reduced ability of the stream ecosystems to resist environmental changes. Nutrient enrichment also increased the δ15N ratios of periphyton and macroinvertebrates, indicating potential δ15N enrichment of stream benthos by nitrogen pollution. Our results suggested that an increase in light intensity due to riparian canopy openness and stream water nutrient enrichment primarily from human activities have interactive effects on resource flow and trophic structure in stream food webs.

Changes in the composition of rhizosphere bacterial communities in response to soil types and acid rain.

It is widely known how acid rain negatively impacts plant physiology. However, the magnitude of these effects may depend on soil types. Although the response of aboveground parts has received much attention, the effects of soil types and acid rain on underground processes are yet to be studied, specifically with respect to the composition and diversity of bacterial communities in the rhizosphere. Based on a high throughput sequencing approach, this study examined how different soil types, acid rain of different pH, and interactions between the two factors influenced the growth and rhizosphere bacterial communities of Jatropha curcas L. The present study pointed out that the soil pH, total nitrogen (TN), total phosphorus (TP), total potassium (TK), and total organic carbon/total nitrogen (C/N) were more related to soil type than to acid rain. The growth of J. curcas aboveground was mainly affected by acid rain, while the underground growth was mainly influenced by soil type. Changes in bacterial abundance indicated that the genera (Burkholderia-Paraburkholde, Bryobacter, Cupriavidus, Mycobacterium, and Leptospirillu) and phyla (Acidobacteria and Actinobacteria) could likely resist acid rain to some extent, with Acidobacteria, Gemmatimonadetes and Proteobacteria being well adapted to the copiotrophic environments. Results of correlational analyses between Firmicutes and soil properties (pH, TN, TK) further indicated that this phylum was also well adapted to a nutrient-deficient habitat of low pH. Finally, while Mycobacterium and Bradyrhizobium could adapt to low pH, high soil TK contents were not conducive to their enrichment. The results also showed that acid rain shifted the bacterial groups from fast-growing copiotrophic populations to slow-growing oligotrophic ones. The RDA analysis, and Pearson's rank correlation coefficients indicated that soil pH and TK were the main factors influencing bacterial richness.

Anthropogenic impacts on the nitrate pollution in an urban river: Insights from a combination of natural-abundance and paired isotopes.

Urban rivers are often characterized by high nitrate (NO3-) loadings. High NO3- loadings cause water quality and ecological damages, which undermines the sustainable development of cities. To date, the drivers of these high NO3- loadings remain unclear. This study, for the first time, integrated natural-abundance isotopes (δ15 N/δ18O-NO3- and δD/δ18O-H2O) and 15N-pairing techniques to comprehensively reveal the anthropogenic impacts on the NO3- pollution in an urban river. Natural-abundance isotopes suggested that in both the wet and dry seasons, the NO3- was predominantly from the conservative mixing of different sources, and biological NO3- removal was minor. The 15N-pairing experiments supported the natural-abundance isotope data, quantitatively showing that in-soil nitrification was prevailing, while NO3- removal processes (denitrification, anammox, and dissimilatory NO3- reduction to ammonium) were weak. A Bayesian isotope-mixing model showed that soil sources (soil organic nitrogen and chemical fertilizer) dominated the NO3- in the upper reaches, while in the lower reaches, the impermeable riparian zone short-circuited the access of soils to the river. Here, the wastewater treatment plants became a significant source of NO3-. This study quantitatively revealed the drivers of high NO3- loadings in an urban river, and generated important clues for effective NO3- pollution control and remediation in urban rivers.

Effects of national ecological restoration projects on carbon sequestration in China from 2001 to 2010.

The long-term stressful utilization of forests and grasslands has led to ecosystem degradation and C loss. Since the late 1970s China has launched six key national ecological restoration projects to protect its environment and restore degraded ecosystems. Here, we conducted a large-scale field investigation and a literature survey of biomass and soil C in China's forest, shrubland, and grassland ecosystems across the regions where the six projects were implemented (∼16% of the country's land area). We investigated the changes in the C stocks of these ecosystems to evaluate the contributions of the projects to the country's C sink between 2001 and 2010. Over this decade, we estimated that the total annual C sink in the project region was 132 Tg C per y (1 Tg = 1012 g), over half of which (74 Tg C per y, 56%) was attributed to the implementation of the projects. Our results demonstrate that these restoration projects have substantially contributed to CO2 mitigation in China.

Ecophysiological responses of Jatropha curcas L. seedlings to simulated acid rain under different soil types.

Acid rain is a global environmental problem. Acid rain can affect plants directly by damaging the leaves and indirectly by soil acidifying. Many studies have been conducted to investigate the impacts of acid rain on plant under a single soil type. However, there is little information on the effect of acid rain on plant under different soil types. Jatropha curcas L. is an energy plant widely distributed in acid rain pollution area with various soil types. In this study, we investigated the effects of acid rain (pH2.5, pH3.5, pH4.5, pH5.6) on the growth, physiology, nutrient elements and bacterial community of J. curcas seedlings under different soil types [Red soils (RS), Yellow soils (YS), Yellow-brown soils (YBS), and Purplish soils (PS)]. Acid rain and soil types significantly influence the growth of J. curcas seedlings, and there was a significant interaction between acid rain and soil types. Acid rain (pH 4.5) was beneficial to the growth of J. curcas seedlings, whereas acid rain (pH 2.5 or 3.5) inhibited growth of J. curcas seedlings. The growth of J. curcas seedlings could resist the stress of acid rain by scavenging and detoxification of active oxygen species in leaves. Combined with the increase in relative growth rate of seedlings treated with simulated acid rain at pH 4.5, we inferred that K can stimulate the growth of seedlings. The lower soil pH, cation exchange capacity and base saturation had stronger inhibitory effects on growth of J. curcas seedlings. YBS and PS were beneficial for growth of J. curcas seedlings by higher buffering capacity under acid rain treatments. The phylum Proteobacteria was found to predominate in rhizosphere soils. YBS was favorable to support Proteobacteria growth and reproduction. The redundancy analysis showed that the Cyanobacteria were favorable to growth of J. curcas seedlings.

Transformation and source identification of N in the upper reaches of the Han River basin, China: evaluated by a stable isotope approach.

Given the spatial and temporal variability in hydrological conditions and nitrogen (N) processes, it is of great uncertainty to identify the N sources and evaluate N transformation processes in the upper Han River. Investigations were conducted in November 2015 and January, April, and July 2016, using an isotopic method and water quality monitoring. The significant and positive correlation between NO3- concentrations and Cl- (p < 0.01) in most sampling months suggested that the great influence of human activities and sewage or manure was the dominant NO3- source. The δ15NO3- values and NO3-/Cl- variations indicated that riverine N mainly came from soil organic N and sewage in November. Fertilizer and sewage were the major N sources in January and April, respectively. In July, water was influenced by various N inputs. The nitrification process played an important role in the low δ15NO3- values in January, while both nitrification and plant uptake resulted in the increase in δ15NH4+ values in April. The simultaneous effect of N fixation and plant uptake maintained the stabilization of δ15NH4+ concentrations. Our study provides theoretical basis on N sources and transformations for controlling N pollution and improving water quality in the upper Han River in the near future.

Global-scale patterns of nutrient density and partitioning in forests in relation to climate.

Knowledge of nutrient storage and partitioning in forests is imperative for ecosystem models and ecological theory. Whether the nutrients (N, P, K, Ca, and Mg) stored in forest biomass and their partitioning patterns vary systematically across climatic gradients remains unknown. Here, we explored the global-scale patterns of nutrient density and partitioning using a newly compiled dataset including 372 forest stands. We found that temperature and precipitation were key factors driving the nutrients stored in living biomass of forests at global scale. The N, K, and Mg stored in living biomass tended to be greater in increasingly warm climates. The mean biomass N density was 577.0, 530.4, 513.2, and 336.7 kg/ha for tropical, subtropical, temperate, and boreal forests, respectively. Around 76% of the variation in biomass N density could be accounted by the empirical model combining biomass density, phylogeny (i.e., angiosperm, gymnosperm), and the interaction of mean annual temperature and precipitation. Climate, stand age, and biomass density significantly affected nutrients partitioning at forest community level. The fractional distribution of nutrients to roots decreased significantly with temperature, suggesting that forests in cold climates allocate greater nutrients to roots. Gymnosperm forests tended to allocate more nutrients to leaves as compared with angiosperm forests, whereas the angiosperm forests distributed more nutrients in stems. The nutrient-based Root:Shoot ratios (R:S), averaged 0.30 for R:SN , 0.36 for R:SP , 0.32 for R:SK , 0.27 for R:SCa , and 0.35 for R:SMg , respectively. The scaling exponents of the relationships describing root nutrients as a function of shoot nutrients were more than 1.0, suggesting that as nutrient allocated to shoot increases, nutrient allocated to roots increases faster than linearly with nutrient in shoot. Soil type significantly affected the total N, P, K, Ca, and Mg stored in living biomass of forests, and the Acrisols group displayed the lowest P, K, Ca, and Mg.

Edaphic Conditions Regulate Denitrification Directly and Indirectly by Altering Denitrifier Abundance in Wetlands along the Han River, China.

Riparian wetlands play a critical role in retaining nitrogen (N) from upland runoff and improving river water quality, mainly through biological processes such as soil denitrification. However, the relative contribution of abiotic and biotic factors to riparian denitrification capacity remains elusive. Here we report the spatiotemporal dynamics of potential and unamended soil denitrification rates in 20 wetlands along the Han River, an important water source in central China. We also quantified the abundance of soil denitrifying microorganisms using nirK and nirS genes. Results showed that soil denitrification rates were significantly different between riparian and reservoir shoreline wetlands, but not between mountain and lowland wetlands. In addition, soil denitrification rates showed strong seasonality, with higher values in August (summer) and April (spring) but lower values in January (winter). The potential and unamended denitrification rates were positively correlated with edaphic conditions (moisture and carbon concentration), denitrifier abundance, and plant species richness. Path analysis further revealed that edaphic conditions could regulate denitrification rates both directly and indirectly through their effects on denitrifier abundance. Our findings highlight that not only environmental factors, but also biotic factors including denitrifying microorganisms and standing vegetation, play an important role in regulating denitrification rate and N removal capacity in riparian wetlands.

Response of soil physico-chemical properties to restoration approaches and submergence in the water level fluctuation zone of the Danjiangkou Reservoir, China.

With the completion of the Danjiangkou Dam, the impoundment and drainage of dams can significantly alter shorelines, hydrological regime, and sediment and can result in the loss of soil and original riparian vegetation. Revegetation may affect soil properties and have broad important implications both for ecological services and soil recovery. In this work, we investigated the soil properties under different restoration approaches, and before and after submergence in the water level fluctuation zone (WLFZ) of the Danjiangkou Reservoir. Soil physical (bulk density and soil moisture), chemical (pH, soil organic carbon, nitrogen, phosphorus and potassium contents), and heavy metals were determined. This study reported that restoration approaches have impacts on soil moisture, pH, N, soil organic carbon, P, K and heavy metals in the WLFZ of the Danjiangkou Reservoir. Our results indicated that different restoration approaches could increase the soil moisture while decrease soil pH. Higher soil organic carbon in propagule banks transplantation (PBT) and shrubs restoration (SR) indicate that PBT and SR may provide soil organic matter more quickly than trees restoration (TR). SR and TR could significantly improve the soil total P and available P. PBT and SR could improve the soil total K and available K. SR and TR could significantly promote Cu and Zn adsorption, and Pb and Fe release by plant. Submergence could significantly affect the soil pH, NO3－-N, NH4＋-N, total P and available P. Submergence could promote NO3－-N and available P adsorption, and NH4＋-N and total P release by soil. The soil quality index (SQI) values implied that TR and PBT greatly improved soil quality. The present study suggests that PBT and TR could be effective for soil restoration in WLFZ of the Danjiangkou Reservoir.

Down-regulation of tissue N:P ratios in terrestrial plants by elevated CO2.

Increasing atmospheric CO2 concentrations generally alter element stoichiometry in plants. However, a comprehensive evaluation of the elevated CO2 impact on plant nitrogen: phosphorus (N:P) ratios and the underlying mechanism has not been conducted. We synthesized the results from 112 previously published studies using meta-analysis to evaluate the effects of elevated CO2 on the N:P ratio of terrestrial plants and to explore the underlying mechanism based on plant growth and soil P dynamics. Our results show that terrestrial plants grown under elevated CO2 had lower N:P ratios in both above- and belowground biomass across different ecosystem types. The response ratio for plant N:P was negatively correlated with the response ratio for plant growth in croplands and grasslands, and showed a stronger relationship for P than for N. In addition, the CO2-induced down-regulation of plant N:P was accompanied by 19.3% and 4.2% increases in soil phosphatase activity and labile P, respectively, and a 10.1% decrease in total soil P. Our results show that down-regulation of plant N:P under elevated CO2 corresponds with accelerated soil P cycling. These findings should be useful for better understanding of terrestrial plant stoichiometry in response to elevated CO2 and of the underlying mechanisms affecting nutrient dynamics under climate change.

Soil carbon dynamics following land-use change varied with temperature and precipitation gradients: evidence from stable isotopes.

Knowledge of soil organic matter (SOM) dynamics following deforestation or reforestation is essential for evaluating carbon (C) budgets and cycle at regional or global scales. Worldwide land-use changes involving conversion of vegetation with different photosynthetic pathways (e.g. C3 and C4 ) offer a unique opportunity to quantify SOM decomposition rate and its response to climatic conditions using stable isotope techniques. We synthesized the results from 131 sites (including 87 deforestation observations and 44 reforestation observations) which were compiled from 36 published papers in the literatures as well as our observations in China's Qinling Mountains. Based on the 13 C natural abundance analysis, we evaluated the dynamics of new and old C in top soil (0-20 cm) following land-use change and analyzed the relationships between soil organic C (SOC) decomposition rates and climatic factors. We found that SOC decomposition rates increased significantly with mean annual temperature and precipitation in the reforestation sites, and they were not related to any climatic factor in deforestation sites. The mean annual temperature explained 56% of variation in SOC decomposition rates by exponential model (y = 0.0014e0.1395x ) in the reforestation sites. The proportion of new soil C increased following deforestation and reforestation, whereas the old soil C showed an opposite trend. The proportion of new soil C exceeded the proportion of old soil C after 45.4 years' reforestation and 43.4 years' deforestation, respectively. The rates of new soil C accumulation increased significantly with mean annual precipitation and temperature in the reforestation sites, yet only significantly increased with mean annual precipitation in the deforestation sites. Overall, our study provides evidence that SOC decomposition rates vary with temperature and precipitation, and thereby implies that global warming may accelerate SOM decomposition.

Development of a benthic diatom index of biotic integrity (BD-IBI) for ecosystem health assessment of human dominant subtropical rivers, China.

As efforts intensify to address the issues of declining water quality and biodiversity losses in freshwater ecosystems, there have been great demands for effective methods of evaluating aquatic ecosystem health. In this study, benthic algae assemblages and water quality variables were analyzed to develop a benthic diatom-based index of biotic integrity (BD-IBI) for assessment of the aquatic environment in the upper Han River (China). Through the use of multivariate and multimetric approaches, four metrics - % prostrate individuals, % Amphora individuals, % polysaprob species, and diatom-based eutrophication/pollution index (EPI-D) - were identified from 98 candidate metrics to develop a BD-IBI. Application of the index revealed that water quality in 11% of the 31 sampled sites could be described as excellent condition, in 43% of the sites it could be described as good condition, in 25% as moderate condition, and in 21% as poor condition. The assessment further revealed that the main reason for degradation of the Han river ecosystem was nutrient enrichment through agricultural land use.

Lead induced changes in growth and micronutrient uptake of Jatropha curcas L.

Effects of lead treatment on growth and micronutrient uptake in Jatropha curcas L. seedlings were assessed by means of microcosm experiments. Results suggested that superoxide dismutase (SOD) activity increased with increasing lead concentration. There was significant positive correlation between lead treatment concentration and SOD and peroxidase activity. Catalase activity was initiated under lower lead stress but, was inhibited under higher lead exposure. Lead had a stimulating effect on seedlings height and leaf area at lower lead concentrations. The J. curcas can accumulate higher amounts of available lead from soil but can translocate only low amounts to the shoots. Results indicating SOD and peroxidase activity in J. curcas seedlings played an important role in resisting the oxidative stress induced by lead. The addition of lead significantly increased the content of zinc in plant tissue and enhanced the transport of iron from roots to shoots but contributed to a decrease in measured copper, iron, and manganese content.

Lithology constrains the origination of autochthonous carbon in river ecosystems.

Dissolved inorganic carbon (DIC) provides a substrate for primary production in the lotic ecosystems, yet carbon's biogeochemical origination in the lotic food webs is still poorly constrained. Here, we assembled a global dataset of isotopic composition (i.e., 13C/12C or δ13C) of DIC and periphyton (algae being the primary producers) in river waters, and carried out a field study in two catchments respectively with carbonate and silicate dominated lithologies on the Tibetan Plateau. A two-endmember mixing model based on the datasets indicated that δ13C and concentrations of DIC in the river waters were largely determined by the catchment-scale chemical weathering of different lithologies. Meanwhile, a significant correlation was obtained between δ13C-DIC and δ13C-periphyton in the datasets, strongly implying that the origination of periphyton carbon was largely regulated by the catchment lithologies. The δ13C-periphyton compositions are also affected by isotopic fractionations during algal primary production, which, in turn, were closely related to the relationships between primary productivity and DIC availability in the rivers. The study advances our understanding of the origination and transfer of carbon biogeochemically bridging the geosphere and biosphere in the lotic ecosystems.