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In this study, we conducted molecular dynamic simulations to investigate the thermal expansion behavior of Janus MoSSe nanotubes. We focused on understanding how the intrinsic strain in these nanotubes affects their thermal expansion coefficient (TEC). Interestingly, we found that Janus MoSSe nanotubes with sulfur (S) on the outer surface (MoSeS) exhibit a different intrinsic strain compared to those with selenium (Se) on the outer surface (MoSSe). In light of this observation, we explored the influence of this intrinsic strain on the TEC of the nanotubes. Our results revealed distinct trends for the TEC along the radial direction (TEC-r) and the axial direction (TEC-lx) of the MoSSe and MoSeS nanotubes. The TEC-rof MoSeS nanotubes was found to be significantly greater than that of MoSSe nanotubes. Moreover, the TEC-lxof MoSeS nanotubes was smaller than that of MoSSe nanotubes. Further analysis showed that the TEC-rof MoSeS nanotubes decreased by up to 37% as the radius increased, while that of MoSSe nanotubes exhibited a slight increase with increasing radius. On the other hand, the TEC-lxof MoSeS nanotubes increased by as much as 45% with increasing radius, whereas that of MoSSe nanotubes decreased gradually. These opposite tendencies of the TECs with respect to the radius were attributed to the presence of intrinsic strain within the nanotubes. The intrinsic strain was found to play a crucial role in inducing thermally induced bending and elliptization of the nanotubes' cross-section. These effects are considered key mechanisms through which intrinsic strain influences the TEC. Overall, our study provides valuable insights into the thermal stability of Janus nanotubes. By understanding the relationship between intrinsic strain and the thermal expansion behavior of nanotubes, we contribute to the broader understanding of these materials and their potential applications.
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Insect gut bacteria play an essential role in the nutritional metabolism, growth, and development of insects. Grasshoppers (Orthoptera) are cellulose-rich plant-feeding pests. Although the biological potential of grasshopper gut microorganisms to assist cellulose decomposition is well established, microbial resources for efficient degradation of cellulose biomass are still scarce and need to be developed. In this study, we used selective media to isolate cellulose-degrading bacteria from the intestines of Atractomorpha sinensis, Trilophidia annulata, Sphingonotus mongolicus, and Calliptamus abbreviatus. Phylogenetic analysis based on the maximum likelihood method using 16S rDNA sequencing sequences to identify bacteria revealed the isolation of 11 strains belonging to 3 genera, including Klebsiella, Aeromonas, and Bacillus. The degradability of the isolates to cellulose was then determined by the DNS colorimetric method, and the results showed that Bacillus had the highest degradation rate. The elucidation of microbial cellulose degradation capacity in grasshoppers not only contributes to the understanding of multiple plant-insect-microbe interactions, but also provides a valuable microbial resource for solving the biomass conversion of cellulose species problem.
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Gafanhotos , Animais , Gafanhotos/metabolismo , Filogenia , Celulose/metabolismo , Bactérias/genética , BiomassaRESUMO
Some methoxy-, hydroxyl-, pyridyl-, or fluoro-substituted 3,5-bis(arylidene)-4-piperidones (BAPs) could reduce inflammation and promote hepatoma cell apoptosis by inhibiting activation of NF-κB, especially after introduction of trifluoromethyl. Herein, a series of trifluoromethyl-substituted BAPs (4-30) were synthesised and the biological activities were evaluated. We successfully found the most potential 16, which contains three trifluoromethyl substituents and exhibits the best anti-tumour and anti-inflammatory activities. Preliminary mechanism research revealed that 16 could promote HepG2 cell apoptosis in a dose-dependent manner by down-regulating the expression of Bcl-2 and up-regulating the expression of Bax, C-caspase-3. Meanwhile, 16 inhibited activation of NF-κB by directly inhibiting the phosphorylation of p65 and IκBα induced by LPS, together with indirectly inhibiting MAPK pathway, thereby exhibiting both anti-hepatoma and anti-inflammatory activities. Molecular docking confirmed that 16 could bind to the active sites of Bcl-2, p65, and p38 reasonably. The above results suggested that 16 has enormous potential to be developed as a multifunctional agent for the clinical treatment of liver cancers and inflammatory diseases.
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Anti-Inflamatórios não Esteroides/farmacologia , Antineoplásicos/farmacologia , Carcinoma Hepatocelular/tratamento farmacológico , Neoplasias Hepáticas/tratamento farmacológico , NF-kappa B/antagonistas & inibidores , Piperidonas/farmacologia , Anti-Inflamatórios não Esteroides/síntese química , Anti-Inflamatórios não Esteroides/química , Antineoplásicos/síntese química , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Simulação de Acoplamento Molecular , Estrutura Molecular , NF-kappa B/metabolismo , Fosforilação/efeitos dos fármacos , Piperidonas/síntese química , Piperidonas/química , Relação Estrutura-AtividadeRESUMO
Brønsted acid-catalyzed general Petasis allylation and isoprenylation of unactivated ketones were developed by using o-hydroxyaniline and the corresponding pinacolyl boronic esters. This robust methodology provided access to a broad variety of quaternary homoallylic amines and dienyl amines in high yields, proved to be applicable to a gram-scale synthesis, and allowed the synthesis of a potentially bioactive quaternary homoallylic aminodiol.
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Iron oxide nanoparticles are nanomaterials that are used extensively in the biomedical field, but they are associated with adverse effects, including mitochondrial toxicity. Mitochondrial homeostasis is achieved through dynamic stability based on two sets of antagonistic balanced processes: mitochondrial biogenesis and degradation as well as mitochondrial fission and fusion. In this study, we showed that PEG-COOH-coated Fe3 O4 (PEG-Fe3 O4 ) nanoparticles induced mitochondrial instability in dendritic cells (DCs) by impairing mitochondrial dynamics due to promotion of mitochondrial biogenesis through activation of the peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α) pathway, inhibiting mitochondrial degradation via decreased autophagy, and facilitating mitochondrial fragmentation involving increased levels of DRP1 and MFN2. The resulting reduced levels of dextran uptake, CD80, CD86 and chemokine receptor 7 (CCR7) suggested that PEG-Fe3 O4 nanoparticles impaired the functionally immature state of DCs. Autophagy inhibitor 3-methyladenine (3-MA) alleviated PEG-Fe3 O4 nanoparticle-induced mitochondrial instability and impairment of the functionally immature state of DCs due to unexpected enhancement of PGC1α/MFN2-mediated coordination of mitochondrial biogenesis and fusion.
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Adenina/análogos & derivados , Autofagia/efeitos dos fármacos , Células Dendríticas/efeitos dos fármacos , Nanopartículas Magnéticas de Óxido de Ferro/toxicidade , Mitocôndrias/efeitos dos fármacos , Dinâmica Mitocondrial/efeitos dos fármacos , Adenina/farmacologia , Animais , Apoptose/efeitos dos fármacos , Células Cultivadas , Células Dendríticas/metabolismo , Células Dendríticas/patologia , Endocitose/efeitos dos fármacos , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Mitocôndrias/metabolismo , Mitocôndrias/patologia , FenótipoRESUMO
This study investigated the lifetime prevalence of suicide attempts (SA) and independent demographic and clinical correlates in stabilized schizophrenia inpatients. A cross-sectional study was conducted in three psychiatric hospitals in Anhui province, an agricultural province located in east China. Psychopathology and depressive symptoms were assessed using the Positive and Negative Syndrome Scale (PANSS) and Hamilton Depression Rating Scale (HAMD), respectively. A total of 315 stable schizophrenia inpatients were interviewed prior to discharge. The lifetime prevalence of SA was 22.2%. Multiple logistic regression analysis revealed that female gender (P < 0.001, OR = 3.4, 95%CI: 1.9-6.0), being married (P = 0.02, OR = 2.2, 95%CI: 1.1-4.4) and having more severe depressive symptoms (P = 0.014, OR = 1.2, 95%CI: 1.01-1.3) were independently and significantly associated with higher risk of SA. Lifetime SA is common among hospitalized schizophrenia patients living in agricultural areas of China. For suicide prevention, regular assessments, appropriate interventions and clinical management should be integrated into a community-based psychiatric service model for this population.
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Agricultura , Depressão/epidemiologia , População Rural/estatística & dados numéricos , Esquizofrenia/epidemiologia , Tentativa de Suicídio/estatística & dados numéricos , Adulto , China/epidemiologia , Doença Crônica/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , PrevalênciaRESUMO
BACKGROUND: In the early stage of severe burn, patients often exhibit a high level of inflammatory mediators in blood and are likely to develop sepsis. High-volume haemofiltration (HVHF) can eliminate these inflammatory mediators. We hypothesised that early application of HVHF may be beneficial in reducing sepsis and improving the prognosis of patients with severe burns. METHODS: Adults patients with burns ≥ 50% total burn surface area (TBSA) and in whom the sum of deep partial and full-thickness burn areas was ≥ 30% were enrolled in this randomised prospective study, and they were divided into control (41 cases) and HVHF (41 cases) groups. Patients in the control group received standard management for major burns, whereas the HVHF group additionally received HVHF treatment (65 ml/kg/h for 3 consecutive days) within 3 days after burn. The incidence of sepsis and mortality, some laboratory data, levels of inflammatory cytokines in the blood, HLA-DR expression on CD14+ peripheral blood monocytes, the proportion of CD25+Foxp3+ in CD4+ T lymphocytes, and the counts of CD3+, CD4+ and CD8+ T lymphocytes were recorded within 28 days post-burn. RESULTS: The incidence of sepsis, septic shock and duration of vasopressor treatment were decreased significantly in the HVHF group. In addition, in the subgroup of patients with burns ≥ 80% TBSA, the 90-day mortality showed significant decreases in the HVHF group. The ratio of arterial oxygen partial pressure to the fraction of inspiration oxygen was improved after HVHF treatment. In the patients who received HVHF treatment, the blood levels of inflammatory cytokines, including tumour necrosis factor-α, interleukin (IL)-1ß, IL-6 and IL-8, as well as the blood level of procalcitonin were found to be lower than in the control group. Moreover, higher HLA-DR expression on CD14+ monocytes and a lower proportion of CD25+Foxp3+ in CD4+ T lymphocytes were observed in the patients in the HVHF group. CONCLUSIONS: Early application of HVHF benefits patients with severe burns, especially for those with a greater burn area (≥ 80% TBSA), decreasing the incidence of sepsis and mortality. This effect may be attributed to its early clearance of inflammatory mediators and the recovery of the patient's immune status. TRIAL REGISTRATION: Chinese Clinical Trial Register, ChiCTR-TRC-12002616 . Registered on 24 October 2012.
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Queimaduras/complicações , Hemofiltração/normas , Sepse/terapia , Adulto , Queimaduras/mortalidade , Queimaduras/terapia , Citocinas/análise , Citocinas/sangue , Feminino , Hemofiltração/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Escores de Disfunção Orgânica , Pró-Calcitonina/análise , Pró-Calcitonina/sangue , Prognóstico , Estudos Prospectivos , Prevenção Secundária/métodos , Prevenção Secundária/normas , Sepse/etiologia , Sepse/mortalidade , Índice de Gravidade de Doença , Estatísticas não ParamétricasRESUMO
In acidic Cd-contaminated soils, soil nitrogen conversion is inhibited and usually block nitrogen supply for plants. Earthworms are well known for improving soil properties and regulating various soil biogeochemical processes including nitrogen cycling. To figure out the effect and mechanisms of earthworms on soil nitrogen transformation in Cd-contaminated soil, ten treatments with and without A. robustus in five soil Cd concentration gradients were established. The tolerant concentration of A. robustus to Cd in the acidic soil is about 6â¯mgâ¯kg-1. The potential ammonia oxidation of the acidic soils was very low, ranging from 0.05 to 0.1⯵g NO2--N g-1 d-1. Although AOA was more abundant in the acidic soil than AOB, AOA was inhibited by Cd pollution, while AOB showed some increase under Cd-stress. AOA may play a dominant role in ammonia oxidation in acidic soil, but the recovery of nitrification in Cd-contaminated acidic soil was probably due to the effect of AOB. Earthworms significantly increased soil pH, DOC, ammonium and PAO, thus promoted soil ammonification and potential nitrification, but had no significant effect on soil net nitrification. Correlation analysis results demonstrate that earthworms may promote soil PAO by increasing soil pH, NH4+-N content, and AOB abundance. This study could provide a theoretical basis for solving the problem of nitrogen-cycling-functional degradation and nitrogen supply in the process of phytoremediation of heavy metals-contaminated soils.
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Cádmio/análise , Nitrogênio/metabolismo , Oligoquetos/metabolismo , Poluentes do Solo/análise , Animais , Archaea/metabolismo , Cádmio/química , Recuperação e Remediação Ambiental , Nitrificação , Oxirredução , Solo/química , Microbiologia do Solo , Poluentes do Solo/químicaRESUMO
Manganese-enhanced MRI studies have proven to be useful in monitoring physiological activities associated with calcium ions (Ca(2+)) due to the paramagnetic property of the manganese ion (Mn(2+)), which makes it an excellent probe of Ca(2+) . In this study, we developed a method in which a Mn(2+)-enhanced T1 -map MRI could enable the monitoring of Ca(2+) influx during the early stages of intestinal ischemia-reperfusion (I/R) injury. The Mn(2+) infusion protocol was optimized by obtaining dose-dependent and time-course wash-out curves using a Mn(2+)-enhanced T1-map MRI of rabbit abdomens following an intravenous infusion of 50 mmol/l MnCl2 (5-10 nmol/g body weight (BW)). In the rabbit model of intestinal I/R injury, T1 values were derived from the T1 maps in the intestinal wall region and revealed a relationship between the dose of the infused MnCl2 and the intestinal wall relaxation time. Significant Mn(2+) clearance was also observed over time in control animals after the infusion of Mn(2+) at a dose of 10 nmol/g BW. This technique was also shown to be sensitive enough to monitor variations in calcium ion homeostasis in vivo after small intestinal I/R injury. The T1 values of the intestinal I/R group were significantly lower (P < 0.05) than that of the control group at 5, 10, and 15 min after Mn(2+) infusion. Our data suggest that MnCl2 has the potential to be an MRI contrast agent that can be effectively used to monitor changes in intracellular Ca(2+) homeostasis during the early stages of intestinal I/R injury.
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Cálcio/metabolismo , Enteropatias/metabolismo , Intestino Delgado/metabolismo , Imageamento por Ressonância Magnética/métodos , Manganês/farmacocinética , Traumatismo por Reperfusão/metabolismo , Animais , Biomarcadores/metabolismo , Meios de Contraste/farmacocinética , Homeostase , Aumento da Imagem/métodos , Enteropatias/patologia , Intestino Delgado/patologia , Espectroscopia de Ressonância Magnética/métodos , Coelhos , Traumatismo por Reperfusão/patologia , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Distribuição TecidualRESUMO
Using outdoor exposure and cinnamon soil incubation test, by quality changes, infrared spectroscopy and electron microscopic scanning technology, to research the degradation ability of self-developed coated fertilizer films. The results of outdoor exposure and cinnamon soil incubation test showed that all films had certain degradation ability, and the degradation rate increased with the increase of time. Under two kinds of test conditions, the highest degradation rate could reach above 35%. The degradation ability of film citric acid/ PVA was much stronger than epoxy resin/PVA. The degradation ability of citric acid/PVA/diatomite composite film materials was further enhanced because of the addition of diatomite. The epoxy resin/PVA composite film materials, although they had certain degradability, compared to the contrast, the difference was not significant, and adding diatomite can't obviously increase the degradation rate. The results of IR spectroscopy showed that some major functional groups, such as C==O, C==C, ==C--H would be reduced after degradation, and the transmission rate also increased, which showed that the degradation of composite film materials must be happened Scanning electron microscopy showed that the surface becomes rough and uneven, and it also meant the films have some degradation. The results of IR spectroscopy and scanning electron microscopy were consistent with the results of quality change test, and could more objectively represent the degradability of film material. Modified film materials can effectively control nutrient release without causing harm to the soil environment, so it is suitable for the film materials of coated fertilizer.
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OBJECTIVE: Endoscopies are common clinical examinations that are somewhat painful and even cause fear and anxiety for patients. We performed this systematic review and meta-analysis of randomized controlled trials to determine the effect of music on patients undergoing various endoscopic procedures. METHODS: We searched the Cochrane Library, Issue 6, 2013, PubMed, and EMBASE databases up to July 2013. Randomized controlled trials comparing endoscopies, with and without the use of music, were included. Two authors independently abstracted data and assessed risk of bias. Subgroup analyses were performed to examine the impact of music on different types of endoscopic procedures. RESULTS: Twenty-one randomized controlled trials involving 2,134 patients were included. The overall effect of music on patients undergoing a variety of endoscopic procedures significantly improved pain score (weighted mean difference [WMD] = -1.53, 95% confidence interval [CI] [-2.53, -0.53]), anxiety (WMD = -6.04, 95% CI [-9.61, -2.48]), heart rate (P = 0.01), arterial pressure (P < 0.05), and satisfaction score (SMD = 1.83, 95% CI [0.76, 2.91]). Duration of the procedure (P = 0.29), except for gastrointestinal endoscopy (P = 0.03), and sedative or analgesic medication dose (P = 0.23, P = 0.12, respectively) were not significantly decreased in the music group, compared with the control group. Furthermore, music had little effect for patients undergoing colposcopy and bronchoscopy in the subanalysis. CONCLUSION: Our meta-analysis suggested that music may offer benefits for patients undergoing endoscopy, except in colposcopy and bronchoscopy.
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Endoscopia/efeitos adversos , Endoscopia/psicologia , Musicoterapia/métodos , Estresse Psicológico/prevenção & controle , Ansiedade/prevenção & controle , Ansiedade/psicologia , Humanos , Dor/prevenção & controle , Dor/psicologia , Ensaios Clínicos Controlados Aleatórios como Assunto , Estresse Psicológico/psicologiaRESUMO
Background: Colorectal cancer (CRC) is considered the most prevalent synchronous malignancy in patients with gastric cancer. This large retrospective study aims to clarify correlations between gastric histopathology stages and risks of specific colorectal neoplasms, to optimize screening and reduce preventable CRC. Methods: Clinical data of 36,708 patients undergoing gastroscopy and colonoscopy from 2005-2022 were retrospectively analyzed. Correlations between gastric and colorectal histopathology were assessed by multivariate analysis. Outcomes of interest included non-adenomatous polyps (NAP), conventional adenomas (CAs), serrated polyps (SPs), and CRC. Statistical analysis used R version 4.0.4. Results: Older age (≥50 years) and Helicobacter pylori infection (HPI) were associated with increased risks of conventional adenomas (CAs), serrated polyps (SPs), non-adenomatous polyps (NAP), and colorectal cancer (CRC). Moderate to severe intestinal metaplasia specifically increased risks of NAP and CAs by 1.17-fold (95% CI 1.05-1.3) and 1.19-fold (95% CI 1.09-1.31), respectively. For CRC risk, low-grade intraepithelial neoplasia increased risk by 1.41-fold (95% CI 1.08-1.84), while high-grade intraepithelial neoplasia (OR 3.76, 95% CI 2.25-6.29) and gastric cancer (OR 4.81, 95% CI 3.25-7.09) showed strong associations. More advanced gastric pathology was correlated with progressively higher risks of CRC. Conclusion: Precancerous gastric conditions are associated with increased colorectal neoplasm risk. Our findings can inform screening guidelines to target high-risk subgroups, advancing colorectal cancer prevention and reducing disease burden.
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In order to study the effects of temperature, wind speed, and leakage volume on the diffusion of heavy gas leakage, this paper establishes a scaling model for the experimental study of gas leakage and diffusion by using the similarity theory with a certain factory as the target. And carbon dioxide gas is selected to replace the toxic and harmful heavy gas to carry out experiments under different temperatures (0-40 °C), wind speeds (0-2 m/s), and leakage velocities (2.5-12.5 L/min), respectively. The results showed that the diffusion rate of heavy gas expanded with increasing temperature under the conditions of wind speed of 0.25 m/s and leakage velocity of 1.5 L/min. When the temperature was increased from 0 to 40 °C, the concentration increase at each location was 125-290% at 600 s. Under the condition of temperature of 20 °C and leakage velocity of 5 L/min, the concentration at each location increased linearly with diffusion time when there was wind, while the linear relationship was not obvious when there was no wind. The effect on the concentration was larger when the wind speed was less than 1 m/s and smaller when the wind speed was greater than 1 m/s. At 20 °C and a wind speed of 0.5 m/s, the concentration of carbon dioxide at each location was increasing as the leakage increased. As the leakage velocity increases from 2.5 to 12.5 L/min, the carbon dioxide concentration at 600 s spreads 2-14 times. The research in this paper provides some decision support for the rescue work, which is important for improving the emergency rescue capability of the leakage accident.
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Locusta migratoria is an important phytophagous pest, and its gut microbial communities play an important role in cellulose degradation. In this study, the gut microbial and cellulose digestibility dynamics of Locusta migratoria were jointly analyzed using high-throughput sequencing and anthrone colorimetry. The results showed that the gut microbial diversity and cellulose digestibility across life stages were dynamically changing. The species richness of gut bacteria was significantly higher in eggs than in larvae and imago, the species richness and cellulose digestibility of gut bacteria were significantly higher in early larvae (first and second instars) than in late larvae (third to fifth instars), and the diversity of gut bacteria and cellulose digestibility were significantly higher in imago than in late larvae. There is a correlation between the dynamics of gut bacterial communities and cellulose digestibility. Enterobacter, Lactococcus, and Pseudomonas are the most abundant genera throughout all life stages. Six strains of highly efficient cellulolytic bacteria were screened, which were dominant gut bacteria. Carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) experiments revealed that Pseudomonas had the highest cellulase enzyme activity. This study provides a new way for the screening of cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors. IMPORTANCE: Cellulose is the most abundant and cheapest renewable resource in nature, but its degradation is difficult, so finding efficient cellulose degradation methods is an urgent challenge. Locusta migratoria is a large group of agricultural pests, and the large number of microorganisms that inhabit their intestinal tracts play an important role in cellulose degradation. We analyzed the dynamics of Locusta migratoria gut microbial communities and cellulose digestibility using a combination of high-throughput sequencing technology and anthrone colorimetry. The results revealed that the gut microbial diversity and cellulose digestibility were dynamically changed at different life stages. In addition, we explored the intestinal bacterial community of Locusta migratoria across life stages and its correlation with cellulose digestibility. The dominant bacterial genera at different life stages of Locusta migratoria were uncovered and their carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) were determined. This study provides a new avenue for screening cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors.
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Bactérias , Celulose , Microbioma Gastrointestinal , Locusta migratoria , Animais , Celulose/metabolismo , Microbioma Gastrointestinal/fisiologia , Locusta migratoria/microbiologia , Bactérias/metabolismo , Bactérias/genética , Bactérias/isolamento & purificação , Larva/microbiologia , Sequenciamento de Nucleotídeos em Larga Escala , Digestão/fisiologiaRESUMO
A cell carrier plays an important role in the maintenance, growth and engraftment of specific cells aimed for defined therapeutic uses in many tissue engineering strategies. A suitable microenvironment for the cells allows for the maximum efficacy of the hybrid device. We have prepared an injectable thermosensitive chitosan/ß-glycerophosphate/collagen (C/GP/Co) gel and investigated its potential application as a support for the culture of skeletal muscle satellite cells (SMSCs). A cell viability assay was used to evaluate the in vitro cytocompatibility of the gel. Cell growth was assessed by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and histological analysis. The influence of the C/GP/Co gel on the plasticity of SMSCs seeded at the surface of the gel was assessed by induction of the myogenic, osteogenic and adipogenic differentiation. C/GP/Co gel provided the appropriate environment for the culture of SMSCs in vitro. In addition, the C/GP/Co gel supported SMSC plasticity. In vivo testing of the SMSC-seeded gel was investigated by subcutaneous injection into the dorsum of nude mice. Cell viability was assessed both by in vivo imaging and histological examination of the explants. In conclusion, C/GP/Co hydrogel is a cytocompatible carrier for the in vivo delivery of SMSCs and supportive for SMSC plasticity. Thus, this gel has potential applications in tissue engineering and regenerative medicine.
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Adipócitos/efeitos dos fármacos , Hidrogéis/farmacologia , Bombas de Infusão Implantáveis , Músculo Esquelético/efeitos dos fármacos , Osteócitos/efeitos dos fármacos , Células Satélites de Músculo Esquelético/efeitos dos fármacos , Adipócitos/citologia , Animais , Materiais Biocompatíveis , Diferenciação Celular , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Quitosana/química , Colágeno/química , Glicerofosfatos/química , Hidrogéis/química , Injeções Subcutâneas , Masculino , Camundongos , Camundongos Nus , Músculo Esquelético/citologia , Osteócitos/citologia , Células Satélites de Músculo Esquelético/citologia , Temperatura , Engenharia TecidualRESUMO
In the title compound, C31H30N2O, the cis-fused cyclo-hexene and cyclo-octane rings adopt boat and boat-chair conformations, respectively. The essentially planar five-membered N-heterocyclic ring [r.m.s. deviation = 0.017â (1)â Å] is cis- and trans-fused, respectively, with the cyclo-hexene and cyclo-octane rings. In the crystal, mol-ecules are linked into inversion dimers through pairs of weak C-Hâ¯O inter-actions.
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A new species of the genus Hamodes Guenée, 1852, Hamodes zhipengi sp. nov. is described and illustrated from China. The species resembles H. pseudobutleri Wei & Wang, 2019 but differs in wings ground colour; and male genitalia with differently shaped valvae, ampullae and armature of vesica. DNA barcode of the new species and relatives are deposited in GenBank. The holotype is deposited in the Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou.
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Lepidópteros , Mariposas , Masculino , Animais , Lepidópteros/genética , Mariposas/genética , China , Universidades , GenitáliaRESUMO
Gastric cancer (GC) is a prominent global health issue, as it ranks as the fifth most prevalent type of cancer and the fourth most significant cause of cancer-related mortality worldwide. Although H. pylori is known to play a role in the development of GC, genetic factors also play a role in its onset and progression. Recent studies have shown that genetic polymorphisms are strongly associated with the development of GC and that certain single nucleotide polymorphisms (SNPs) can be used as biomarkers for early diagnosis and prevention. Epigenetic disturbances, such as DNA methylation, are involved in the development of GC, and mutations in the DNA methyltransferase (DNMT) gene have been found to increase the risk of GC. However, previous findings on the association between DNMTs SNPs and GC risk have been inconsistent. In this study, an updated meta-analysis of three well-studied and controversial DNMTs polymorphic loci, DNMT1 rs16999593, DNMT3A rs1550117 and DNMT3B rs1569686, was performed to provide more reliable results. It was found that DNMT1 rs16999593 was not associated with GC, DNMT3A rs1550117 may have a positive association with GC risk, and DNMT3B rs1569686 may be a protective factor for GC. These findings may provide valuable information for early diagnosis and prevention of GC, but further studies are needed to confirm these results.
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Predisposição Genética para Doença , Neoplasias Gástricas , Humanos , Genótipo , DNA Metiltransferase 3A , DNA (Citosina-5-)-Metiltransferases/genética , DNA (Citosina-5-)-Metiltransferase 1/genética , Polimorfismo de Nucleotídeo Único , Metilação de DNA , Neoplasias Gástricas/genética , Fatores de ProteçãoRESUMO
A new species Eterusia sinoraja Huang & Horie sp. n. is described from western Sichuan Province, western China. The comparison is made with the reminiscent E. raja Moore, 1859 from northeastern India. The adult and genitalia of both sexes of the aforementioned taxa are illustrated.
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Lepidópteros , Mariposas , Masculino , Feminino , Animais , Genitália , ChinaRESUMO
The name Striatochrista nom. n. is introduced as replacement for Striatella Volynkin & S.-Y. Huang, 2019. A new genus, Letrasilta S.-Y. Huang & Volynkin gen. n. is erected to include the Striatella cernyi species-group with the new species, L. ratnasambhava S.-Y. Huang, Volynkin & Yin sp. n. from Xizang, southwestern China designated as the type species. Based on the molecular phylogenetic analysis, the new genus is found to be sister to the clade (Aberrasine + ((Indiania + Idopterum) + Striatochrista nom. n.)) but is distinguished from all the relevant genera by the unique genitalia features. Letrasilta cernyi (Volynkin, 2018) comb. n. is also reported from India for the first time. Adults and genitalia of the aforementioned taxa are illustrated. A checklist of the genus Striatochrista is also provided.