RESUMO
The development of sustainable advanced energy conversion technologies and efficient pollutant treatment processes is a viable solution to the two global crises of the lack of non-renewable energy resources and environmental harm. In recent years, the interaction of biological and chemical oxidation units to utilize biomass has been extensively studied. Among these systems, bio-electro-Fenton (BEF) and photo-bio-electro-Fenton (PBEF) systems have shown prospects for application due to making rational and practical conversion and use of energy. This review compared and analyzed the electron transfer mechanisms in BEF and PBEF systems, and systematically summarized the techniques for enhancing system performance based on the generation, transfer, and utilization of electrons, including increasing the anode electron recovery efficiency, enhancing the generation of reactive oxygen species, and optimizing operational modes. This review compared the effects of different methods on the electron flow process and fully evaluated the benefits and drawbacks. This review may provide straightforward suggestions and methods to enhance the performance of BEF and PBEF systems and inspire the reader to explore the generation and utilization of sustainable energy more deeply.
Assuntos
Oxirredução , Peróxido de Hidrogênio/química , Ferro/química , Espécies Reativas de Oxigênio , BiomassaRESUMO
The combined process of biochar (BC) and potassium ferrate (PF) offers a fascinating technique for efficient dewatering of digestate. However, the effects of BC/PF treatment on the dewaterability and mechanisms of FWD are still unknown. This study aimed to reveal the impact mechanisms of BC/PF treatment on digestate dewatering performance. Experimental results indicated that BC/PF treatment significantly enhanced the dewaterability of digestate, with the minimum specific resistance to filtration of (1.05 ± 0.02) × 1015 m·kg-1 and water content of 57.52 ± 0.51% being obtained at the concentrations of 0.018 g·g-1 total solid (TS) BC300 and 0.20 g·g-1 TS PF, which were 8.60% and 13.59% lower than PF treatment, respectively. BC/PF treatment proficiently reduced the fractal dimension, bound water content, apparent viscosity, and gel-like network structure strength of digestate, as well as increased the floc size and zeta potential of digestate. BC/PF treatment promoted the conversion of extracellular polymeric substances (EPS) fractions from inner EPS to soluble EPS, increased the fluorescence intensity of the dissolved compounds, and enhanced the hydrophobicity of proteins. Mechanisms investigations showed that BC/PF enhanced dewatering through non-reactive oxygen species pathways, i.e., via strong oxidative intermediate irons species Fe(V)/Fe(IV). BC/PF treatment enhanced the solubilization of nutrients, the inactivation of fecal coliforms, and the mitigation of heavy metal toxicity. The results suggested that BC/PF treatment is an effective digestate dewatering technology which can provide technological supports to the closed-loop treatment of FWD.
Assuntos
Carvão Vegetal , Perda e Desperdício de Alimentos , Compostos de Ferro , Ferro , Compostos de Potássio , Eliminação de Resíduos , Alimentos , Esgotos/química , Água/química , Eliminação de Resíduos Líquidos/métodosRESUMO
OBJECTIVES: To investigate the potential mechanisms of shikonin in preventing and treating periodontitis using network pharmacology and molecular docking methods. MATERIALS AND METHODS: The targets of shikonin were obtained in TCMSP and SEA databases, and targets of periodontitis were gathered from the OMIM, GeneCards and Drugbank Databases. The intersecting targets were entered into the DAVID database to obtain the relevant biological functions and pathways by GO and KEGG enrichment analysis. The obtained targets were analysed the protein-protein interaction (PPI) in STRING platform. In Cytoscape 3.8.0, the network analysis function with the MCODE plug-in were used to obtain the key targets, of shikonin and periodontitis. Molecular docking and molecular dynamics simulation (MD) were used to assess the affinity between the shikonin and the key targets. RESULTS: Shikonin was screened for 22 targets and periodontitis was screened for 944 targets, the intersecting targets were considered as potential therapeutic targets. The targets played important roles in cellular response to hypoxia, response to xenobiotic stimulus and positive regulates of apoptotic process by GO enrichment analysis. 10 significant pathways were analyzed by KEGG, such as human cytomegalovirus infection and PI3K-Akt signaling pathway, etc. Cytoscape software screened the key genes including AKT1, CCL5, CXCR4, PPARG, PTEN, PTGS2 and TP53. Molecular docking and MD results showed that shikonin could bind stably to the targets. CONCLUSIONS: The present study enriched the molecular mechanisms in periodontitis with shikonin, providing potential therapeutic targets for periodontitis.
Assuntos
Simulação de Acoplamento Molecular , Naftoquinonas , Farmacologia em Rede , Periodontite , Naftoquinonas/farmacologia , Naftoquinonas/uso terapêutico , Periodontite/tratamento farmacológico , Humanos , Mapas de Interação de Proteínas , Simulação de Dinâmica Molecular , Transdução de Sinais/efeitos dos fármacosRESUMO
OBJECTIVE: This study aimed to identify a quantitative index for optical coherence tomography (OCT) images to discriminate tumours from surrounding tissues. SUBJECTS AND METHODS: Based on OCT measurements, mean grey values were determined from 432 locations on fifty-four human tissue specimens (eighteen cancerous, para-cancerous, and normal tissues each). These results were histologically evaluated by hematoxylin and eosin staining (H&E). RESULTS: The mean grey values of oral squamous cell carcinoma (OSCC) measurements were significantly different from those of the surrounding healthy tissue (p value < 0.0001), with the former being higher. The sensitivity and specificity of detecting tumourous tissue using this approach were 93 and 94%, respectively. CONCLUSIONS: OCT as a non-invasive, real-time imaging method, correlates well with H&E pathological images. It can effectively distinguish squamous cell carcinoma from normal tissues with high sensitivity and specificity and is thus expected to assist and guide tumour margin evaluation. CLINICAL RELEVANCE: This discovery highlights the potential of OCT in the objective evaluation of tumour margin during surgery.
Assuntos
Carcinoma de Células Escamosas , Neoplasias Bucais , Sensibilidade e Especificidade , Tomografia de Coerência Óptica , Humanos , Neoplasias Bucais/diagnóstico por imagem , Neoplasias Bucais/patologia , Tomografia de Coerência Óptica/métodos , Carcinoma de Células Escamosas/diagnóstico por imagem , Carcinoma de Células Escamosas/patologia , Mucosa Bucal/diagnóstico por imagem , Mucosa Bucal/patologiaRESUMO
Dissolved organic matter (DOM) plays a pivotal role in the biogeochemical cycles of elements and the regulation of forest ecosystem functions. However, studies on the regional and seasonal characteristics of DOM in cold-temperate montane forests are still not comprehensive. In this study, samples of water, soil, and sediment from different sites in the forest drainage basin were collected, and their DOM was characterized by an excitation-emission matrix and parallel factor analysis (EEM-PARAFAC). The results showed that terrestrial-sourced humic-like substances were the dominant DOM in the studied reservoir and inflowing rivers. The quality and quantity of DOM exhibited spatiotemporal variations with the influence of terrain and monsoonal precipitation. The average concentration of dissolved organic carbon (DOC) in the wet season was 11.62 mg/L, which was higher than that in the dry season (8.18 mg/L). Higher humification index (HIX) values were observed in the wet season and upstream water than in the dry season and reservoir water. Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) was used to further develop a molecular-level understanding of the in situ degradation process of DOM. The results indicated that photodegradation rather than biodegradation may play a dominant role in the in situ degradation of terrestrial-sourced humic-like substances under natural conditions. The biodegradability of DOM was enhanced after the in situ degradation process. Additionally, a significant decrease in the precursors of disinfectant byproducts in DOM was observed after in situ degradation. To our knowledge, this is the first study of the sources, characteristics, and in situ degradation of DOM in a reservoir in a cold-temperate forest. These findings help better understand the quality, quantity, and biogeochemical process of DOM in the studied reservoir and may contribute to the selection of drinking water treatment technologies for water supply.
Assuntos
Matéria Orgânica Dissolvida , Água Potável , Ecossistema , Água Potável/análise , Florestas , Rios/química , Substâncias Húmicas/análise , Espectrometria de FluorescênciaRESUMO
Strengthening direct interspecies electron transfer (DIET), via adding conductive materials, is regarded as an effective way for improving methane productivity of anaerobic digestion (AD). Therein, the supplementation of combined materials (composition of biochar and iron-based materials) has attracted increasing attention in recent years, because of their advantages of promoting organics reduction and accelerating biomass activity. However, as far as we known, there is no study comprehensively summarizing the application of this kind combined materials. Here, the combined methods of biochar and iron-based materials in AD system were introduced, and then the overall performance, potential mechanisms, and microbial contribution were summarized. Furthermore, a comparation of the combinated materials and single material (biochar, zero valent iron, or magnetite) in methane production was also evaluated to highlight the functions of combined materials. Based on these, the challenges and perspectives were proposed to point the development direction of combined materials utilization in AD field, which was hoped to provide a deep insight in engineering application.
Assuntos
Biocombustíveis , Ferro , Anaerobiose , Reatores Biológicos , Metano , EsgotosRESUMO
The easily synthesized, cost-effective, and stable photocatalysts for sulfite activation are always required for the enhancement of organic contaminants degradation. Herein, the facile coprecipitation synthesis of Bismuth oxybromide (BiOBr)/Montmorillonite (MMT) was reported, which could activate sulfite (SO32-/HSO3-) under sunlight and accelerate the catalytic performance more effectively than pristine BiOBr. After adding sulfite to the photocatalysis system, the photodegradation efficiency of atrazine (ATZ) achieved 73.7% ± 1.5% after 5 min and 94.4% ± 1.6% after 30 min of sunlight irradiation with BiOBr/MMT. The BiOBr/MMT-sulfite system also presented remarkable photocatalytic performance to eliminate various contaminants, including ciprofloxacin, sulfadiazine, tetracycline, and carbamazepine. The various features of the photocatalyst materials were studied, including their surface morphology, structure, optical properties, and composition. The results illustrated that by adding MMT, the bandgap of the pristine BiOBr was reduced and the surface area was increased, which led to an increased ability to adsorb materials. Results of various influence factors showed this enhanced system had satisfactory and stable removal performance of ATZ in the pH range of 3.0-6.5, but HPO42- had a strong negative effect on the system performance. Oxysulfur radicals (SO5·- and SO4·-), h+, and 1O2 were discovered as the prevailing active species in the BiOBr/MMT-sulfite system. The proposed degradation mechanism of this photocatalyst-enhanced system revealed that sulfite adsorption on the surface of the photocatalyst played a vital role during the initial phase, and the degradation pathway of ATZ was discussed. This study provides a new synthesis strategy of a photocatalyst for sulfite activation and expands the potential uses of Bi-based photocatalysts in degrading difficult-to-remove organic pollutants.
Assuntos
Bentonita , Luz , Luz Solar , Sulfitos , CatáliseRESUMO
Microstructure surfaces are widely and deeply applied in optical fields to decrease the volume of an optical system and realize sophisticated optical requirements. This paper studies the machining of optical micro-structures with micro-scale height on single crystal germanium by elliptical vibration assisted sculpturing (EVAS) method in which two vibration amplitudes keep constant. Due to the superimposition of low-frequency sculpture path and high-frequency elliptical locus, the investigations are focused on cutting plane to describe motion trajectory of the tool tip of cutting tool for desired complex motion path and to calculate the sculpture path of elliptical vibration cutting device to make corresponding path of tool tip be the envelope of target profile. A comparative experiment is firstly conducted to verify that elliptical locus would deteriorate machining accuracy without accurate compensation for the ellipse. Following that, 2D and 3D sinusoidal surfaces and a picture, whose structural heights are all not less than 2.0µm, are experimentally machined on single crystal germanium. Satisfying experimental results demonstrate the feasibility of EVAS method for fabricating optical micro-structures on germanium.
RESUMO
Optical coherence tomography angiography (OCTA) images suffer from inevitable micromotion (breathing, heartbeat, and blinking) noise. These image artifacts can severely disturb the visibility of results and reduce accuracy of vessel morphological and functional metrics quantization. Herein, we propose a multiple wavelet-FFT algorithm (MW-FFTA) comprising multiple integrated processes combined with wavelet-FFT and minimum reconstruction that can be used to effectively attenuate motion artifacts and significantly improve the precision of quantitative information. We verified the fidelity of image information and reliability of MW-FFTA by the image quality evaluation. The efficiency and robustness of MW-FFTA was validated by the vessel parameters on multi-scene in vivo OCTA imaging. Compared with previous algorithms, our method provides better visual and quantitative results. Therefore, the MW-FFTA possesses the potential capacity to improve the diagnosis of clinical diseases with OCTA.
Assuntos
Artefatos , Tomografia de Coerência Óptica , Tomografia de Coerência Óptica/métodos , Reprodutibilidade dos Testes , Algoritmos , Angiografia/métodosRESUMO
In this study, a microbial fuel cell powered electro-Fenton system (MFCâ EFs) was established in order to overcome the shortcomings of low electron flux and unexpected methane production, while simultaneously treating excess sludge (ES, substrate) and refractory syringic acid (SA). A strategy of co-adding lysozyme (LZ, as ES degradation catalyst) and 2-bromoethanesulfonate (BES, as methane inhibitor) into ES was optimized in MFCâ EFs to maximize electron flux, microbial community diversity and functional gene abundance. The removal of sludge total chemical oxygen demand (TCOD) achieved 81.69% in 25 d under an optimal co-addition strategy (40.41 mg/gSS of LZ, 27.03 mmol/L of BES, adding on 22.8 h of the7th day), with a simultaneous high degradation of SA (99.30% in 25 h). Correspondingly, a maximum power density of 3.35 W/m3 was achieved (only 0.62 W/m3 from the control), which effectively realizes in-situ micro-electricity generation and utilization for bioelectric Fenton processes. Moreover, 42.25% of the total charges were employed for bio-electricity generation. The electricigens of Pseudomonas, Acinetobacter and Chlorobium showed effective enrichment, while the abundance of methanogenesis archaea was extremely decreased. Functional genes associated with methanogenesis including mtaA, hdra, and mcrA were effectively inhibited. The life cycle assessment along with an optimized co-addition strategy illustrated a beneficial environmental effect, particularly in terms of ecosystem quality and climate change. Above all, an enhanced synchronous degradation of excess sludge and refractory pollutants had been realized in a green and environmentally friendly way.
Assuntos
Fontes de Energia Bioelétrica , Poluentes Ambientais , Ácidos Alcanossulfônicos , Ecossistema , Eletricidade , Eletrodos , Elétrons , Metano/metabolismo , Muramidase , EsgotosRESUMO
A bioelectrochemical assisted sludge treatment wetland (BE-STW) is a promising technology used in the elimination of organic compounds and recovery of bio-energy. In this study, four BE-STW systems were constructed to investigate the effects of some substrates (i.e. graphite particles, zeolite, ceramsite, and gravel) on organic compounds biodegradation and transformation, electricity production, and anodic bacterial community. The maximum output voltages were 0.939, 0.870, 0.741 and 0.835 V, and the maximum power densities were 0.467, 0.143, 0.110, and 0.131 W/m3 for the graphite particles (BS-GP), zeolite (BS-Z), ceramsite (BS-C), and gravel (BS-G) systems, respectively. Also, the dissolved organic carbon (DOC) removal rates were 61.84%, 28.54%, 25.56%, and 18.34% in BS-GP, BS-G, BS-Z, and BS-C, respectively. The degradation of aromatic compounds in sludge extracellular biological organic matter (EBOM) was mainly due to the decrease of hydrophilic fraction (HPI) and transphilic acid fraction (TPI-A) contents. Moreover, aromatic proteins were preferentially removed in BS-Z. For BS-C, the tyrosine-like proteins and humic acid-like substances in TPI-A were totally removed. An excitation-emission matrix (EEM) analysis showed that the fluorescent intensity of the humic acid-like substances was the lowest in BS-GP, and no fluorescence peaks of fulvic acid-like substances were observed. Finally, at the genus level, Longilinea, Terrimonas, Ottowia, and Saccharibacteria_genera_incertae_sedis were the dominant bacteria in BE-STW, and Methylophilus was also only detected in BS-GP. These results confirmed that substrate materials have a significant impact on the preferentially degraded organic matter in BE-STWs, which can provide a theoretical basis for the practical application of STW in the future.
Assuntos
Microbiota , Esgotos , Substâncias Húmicas , Compostos Orgânicos , Áreas AlagadasRESUMO
The intensive production and utilization of antibiotics worldwide has inevitably led to releases of very large amounts of these medicines into the environment, and numerous strategies have recently been developed to eliminate antibiotic pollution. Therefore, bismuth-based photocatalysts have attracted much attention due to their high adsorption of visible light and low production cost. This review summarizes the performance, degradation pathways and relevant mechanisms of typical antibiotics during bismuth-based photocatalytic degradation. First, the band gap and redox ability of the bismuth-based catalysts and modified materials (such as morphology, structure mediation, heterojunction construction and element doping) were compared and evaluated. Second, the performance and potential mechanisms of bismuth oxides, bismuth sulfides, bismuth oxyhalides and bismuth-based metal oxides for antibiotic removal were investigated. Third, we analysed the effect of co-existing interfering substances in a real water matrix on the photocatalytic ability, as well as the coupling processes for degradation enhancement. In the last section, current difficulties and future perspectives on photocatalytic degradation for antibiotic elimination by bismuth-based catalysts are summarized. Generally, modified bismuth-based compounds showed better performance than single-component photocatalysts during photocatalytic degradation for most antibiotics, in which h+ played a predominant role among all the related reactive oxygen species. Moreover, the crystal structures and morphologies of bismuth-based catalysts seriously affected their practical efficiencies.
Assuntos
Bismuto , Luz , Antibacterianos , Catálise , OxirreduçãoRESUMO
Diabetic retinopathy (DR) is the most common ocular complication caused by diabetes mellitus and is the main cause of visual impairment in working-age people. Reactive gliosis and pro-inflammatory cytokine production by Müller cells contribute to the progression of DR. Melatonin is a strong anti-inflammatory hormone, mediating the cytoprotective effect of a variety of retinal cells against hyperglycemia. In this study, melatonin inhibited the gliosis activation and inflammatory cytokine production of Müller cells in both in vitro and in vivo models of DR. The melatonin membrane blocker, Luzindole, invalidated the melatonin-mediated protective effect on Müller cells. Furthermore, melatonin inhibited Müller cell activation and pro-inflammatory cytokine production by upregulating the long noncoding RNA maternally expressed gene 3/miR-204/sirtuin 1 axis. In conclusion, our study suggested that melatonin treatment could be a novel therapeutic strategy for DR.
Assuntos
Citocinas/efeitos dos fármacos , Retinopatia Diabética/tratamento farmacológico , Células Ependimogliais/efeitos dos fármacos , Melatonina/uso terapêutico , RNA Longo não Codificante/genética , Animais , Apoptose/efeitos dos fármacos , Citocinas/metabolismo , Diabetes Mellitus/tratamento farmacológico , Diabetes Mellitus/metabolismo , Retinopatia Diabética/metabolismo , Células Ependimogliais/metabolismo , Hiperglicemia/tratamento farmacológico , Hiperglicemia/metabolismo , Inflamação/metabolismo , Camundongos Endogâmicos C57BL , Ativação Transcricional/genéticaRESUMO
On-demand drug release nanoplatforms are promising alternative strategies for enhancing the therapeutic effect of cancer chemotherapy. However, these nanoplatforms still have many drawbacks including rapid blood clearance, nontargeted specificity, and a lack of immune escape function. Even worse, they are also hindered via the dosage-limiting toxicity of traditional chemotherapeutic drugs. Herein, both dual-functional mannose (enhances the antitumor activity of chemotherapeutic drugs and exhibits an innate affinity against the lectin receptor) and amphiphilic d-α-tocopheryl polyethylene glycol 1000 succinate were selected to be covalently linked via a redox-responsive monothioether linkage. The synthesized self-distinguished polymer (TSM), as a structural motif, can be self-assembled into nanoparticles (TSM NPs) in an aqueous solution, in which doxorubicin (DOX) is loaded by weak interactions (TSM-DOX NPs). These TSM-DOX NPs can provide targeted, on-demand drug release under dual stimuli from lysosomal acidity and glutathione (GSH). In addition, TSM-DOX NPs can be self-distinguished via tumor cells in vitro and specifically self-distinguished from the tumor site in vivo. Further in vitro and in vivo research consistently demonstrated that TSM-DOX NPs display highly synergistic chemotherapeutic effects. Taken together, the data show that the self-distinguished GSH-responsive polymer TSM has the potential to load various therapeutic agents.
Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Antibióticos Antineoplásicos/farmacocinética , Doxorrubicina/administração & dosagem , Doxorrubicina/farmacocinética , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Nanopartículas/química , Neoplasias/tratamento farmacológico , Polímeros/química , Animais , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Feminino , Células Hep G2 , Humanos , Células MCF-7 , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Células NIH 3T3 , Ratos , Ratos Sprague-Dawley , Carga Tumoral/efeitos dos fármacos , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
Agriculture-oriented cities in Northeastern China have experienced frequent atmospheric pollution events. Deeper understandings of the pollution characteristics, haze causes and effects of management on local air quality are crucial for conducting integrated management approaches for the sustainable development of agriculture-oriented cities. Taking a typical agriculture-dominant city (i.e., Suihua) in Northeast China, we analyzed in detail the characteristics and causes of atmospheric pollution and evaluated the straw-burning prohibition using multisource data. The results showed a clear temporal pattern of air quality index (AQI) on an annual scale (i.e., 2015-April 2019), with two typical pollution periods occurring in late autumn and early spring. The large areas of concentrated straw burning at local and regional scales accounted for the first period (i.e., October and November), while dust emissions and farming disturbances comprised the second period. The interannual variation in pollution periods among these years was large, showing similar trends from 2015 to 2017 and the postponed late-autumn pollution period in 2018. Our evaluation has shown that the prohibition effect of straw burning significantly improved air quality in 2018, with a reduction of 59% ± 88% in the PM2.5 concentrations in October and November compared to 2015-2017. However, From October to April of the following year, the improvement effect was not significant due to postponement of straw burning to February or March. Our analysis also highlighted the roles of meteorological conditions, Therefore, combined with the promotion of straw utilization, scientifically prescribed burning considering the burning amount and location, meteorological conditions and regional transportation should be implemented.
Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/análise , Agricultura , China , Cidades , Monitoramento Ambiental , Material Particulado/análise , Estações do AnoRESUMO
Elemental tantalum is a well-known biomedical metal in clinics due to its extremely high biocompatibility, which is superior to that of other biomedical metallic materials. Hence, it is of significance to expand the scope of biomedical applications of tantalum. Herein, it is reported that tantalum nanoparticles (Ta NPs), upon surface modification with polyethylene glycol (PEG) molecules via a silane-coupling approach, are employed as a metallic photoacoustic (PA) contrast agent for multiwavelength imaging of tumors. By virtue of the broad optical absorbance from the visible to near-infrared region and high photothermal conversion efficiency (27.9%), PEGylated Ta NPs depict high multiwavelength contrast capability for enhancing PA imaging to satisfy the various demands (penetration depth, background noise, etc.) of clinical diagnosis as needed. Particularly, the PA intensity of the tumor region postinjection is greatly increased by 4.87, 7.47, and 6.87-fold than that of preinjection under 680, 808, and 970 nm laser irradiation, respectively. In addition, Ta NPs with negligible cytotoxicity are capable of eliminating undesirable reactive oxygen species, ensuring the safety for biomedical applications. This work introduces a silane-coupling strategy for the surface engineering of Ta NPs, and highlights the potential of Ta NPs as a biocompatible metallic contrast agent for multiwavelength photoacoustic image.
Assuntos
Meios de Contraste/química , Nanopartículas/química , Neoplasias/diagnóstico , Técnicas Fotoacústicas , Polietilenoglicóis/química , Tantálio/química , Animais , Morte Celular , Linhagem Celular Tumoral , Sobrevivência Celular , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Injeções Intravenosas , Camundongos , Nanopartículas/ultraestrutura , Espectroscopia Fotoeletrônica , Espécies Reativas de Oxigênio/metabolismoRESUMO
High-resolution and real-time visualization of the morphological changes during embryonic development are critical for studying congenital anomalies. Optical coherence tomography (OCT) has been used to investigate the process of embryogenesis. However, the structural visibility of the embryo is decreased with the depth due to signal roll-off and high light scattering. To overcome these obstacles, in this study, combined is a spectral-domain OCT (SD-OCT) with gold nanorods (GNRs) for 2D/3D imaging of live mouse embryos. Inductively coupled plasma mass spectrometry is used to confirm that GNRs can be effectively delivered to the embryos during ex vivo culture. OCT signal, image contrast, and penetration depth are all enhanced on the embryos with GNRs. These results show that after GNR treatment, more accurate spatial localization and better contrasting of the borders among organs can be observed on E9.5 and E10.5 mouse embryos. Furthermore, the strong optical absorbance of GNRs results in much clearer 3D images of the embryos, which can be used for calculating the heart areas and volumes of E9.5 and E10.5 embryos. These findings provide a promising strategy for monitoring organ development and detecting congenital structural abnormalities in mice.
Assuntos
Meios de Contraste/química , Embrião de Mamíferos/diagnóstico por imagem , Ouro/química , Nanopartículas Metálicas/química , Tomografia de Coerência Óptica/métodos , Animais , Meios de Cultura , CamundongosRESUMO
In this study, a novel step-feed three-stage integrated anoxic/oxic biological aerated filter (STIAOBAF) process was developed to enhance nitrogen removal from the synthetic domestic wastewater through optimizing influent flow distribution ratio (IFDR) for three stage reactors (R1, R2, R3). Long-term operation demonstrated that the maximum nitrogen removal efficiency was achieved at the IFDR of 30%:50%:20%. The corresponding effluent total nitrogen (TN) was less than 10â¯mg/L, superior to the first A grade discharge standard of China (Effluent TNâ¯<â¯15â¯mg/L). The IFDR was further optimized to 32%:49%:19% by response surface methodology (RSM) model, thus obtaining the highest TN removal efficiency of 81.4%. Nitrogen profiles suggested the 2nd stage reactor was the greatest significant contributor for nitrogen removal of the whole system. Microbial community analysis revealed that Chloroflexi, Bacteroidetes, Firmicutes, and Acidobacteria were abundant in anoxic zones, while Planctomycetes, Bacteroidetes and Verrucomicrobia were rich in oxic zones. Nitrogen removal-associated functional bacterial groups (Nitrospira, Thauera, Azospira and Candidatus Kuenenia) were also identified, supporting high-rate nitrogen removal through the combination of anoxic denitrification with aerobic simultaneous nitrification and denitrification (SND). The STIAOBAF will offer a compact and robust alternative for advanced nitrogen removal from the sewage.
Assuntos
Nitrogênio , Águas Residuárias , Reatores Biológicos , China , Desnitrificação , Esgotos , Eliminação de Resíduos LíquidosRESUMO
Near-infrared light-mediated theranostic agents with superior tissue penetration and minimal invasion have captivated researchers in cancer research in the past decade. Herein, a probe sonication-assisted liquid exfoliation approach for scalable and continual synthesis of colloidal rhenium disulfide nanosheets, which is further explored as theranostic agents for cancer diagnosis and therapy, is reported. Due to high-Z element of Re (Z = 75) and significant photoacoustic effect, the obtained PVP-capped ReS2 nanosheets are evaluated as bimodality contrast agents for computed tomography and photoacoustic imaging. In addition, utilizing the strong near-infrared absorption and ultrahigh photothermal conversion efficiency (79.2%), ReS2 nanosheets could also serve as therapeutic agents for photothermal ablation of tumors with a tumor elimination rate up to 100%. Importantly, ReS2 nanosheets show no obvious toxicity based on the cytotoxicity assay, serum biochemistry, and histological analysis. This work highlights the potentials of ReS2 nanosheets as a single-component theranostic nanoplatform for bioimaging and antitumor therapy.
Assuntos
Fototerapia/métodos , Rênio/química , Nanomedicina Teranóstica/métodos , Técnicas Fotoacústicas/métodos , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVES: This study aimed to demonstrate the capacity for noninvasive localisation and characterisation of myocardial infarction (MI) in vivo using a hemispherical photoacoustic imaging (PAI) system. MI remains a leading cause of morbidity and mortality worldwide. To enable optimal treatment of patients, timely and accurate diagnosis and longitudinal monitoring is critical. METHODS: Ischaemia was induced in Balb/c mice by ligation of the left anterior descending artery. The hemispherical PAI system, equipped with 128 ultrasonic transducers spirally distributed on the surface, along with parallel data acquisition, was applied for imaging of the mouse heart. RESULTS: Our study showed that hemispherical PAI can delineate thoracic vessels and the morphology of the entire heart. Longitudinal PAI images revealed gradual expansion of the infarcted area along with necrosis and fibrosis, which were quantitatively validated by triphenyltetrazolium chloride staining. After MI modelling, the photoacoustic (PA) signal intensity decreased by 399.1 ± 56.3 (p < 0.001), a ~2.5-fold reduction compared to that of healthy cardiac tissue. The calculated size of the enlarged heart, 10.4 ± 6.0 mm2 (p < 0.001), represents an increase of ~18% versus that of a healthy heart. CONCLUSIONS: PAI enables MI diagnosis and injury localisation with its capabilities for both deep organ imaging and lesion region differentiation. KEY POINTS: ⢠Photoacoustic imaging (PAI), combining optical absorption and ultrasonic resolution, can delineate cardiac anatomy. ⢠PAI can diagnose myocardial infarction lesions with 10 mm imaging depth in vivo. ⢠Quantified results are in excellent agreement with enzyme and histological examinations. ⢠PAI can serve as a complementary modality to SPECT and ultrasound imaging. ⢠This study will encourage further PAI development for clinical use.