Abscisic acid alleviates harmful effect of saline-alkaline stress on tomato seedlings.

Saline-alkaline stress inhibits plant growth and reduces yield. Abscisic acid (ABA) is an important plant hormone in response to plant stress. However, the role of ABA under saline-alkaline stress is poorly understood. Therefore, the mechanisms of ABA accumulation and resistance improvement in tomato seedlings were studied under saline-alkaline stress. We investigated whether ABA accumulation improved the saline-alkaline stress resistance ability of tomato. Here, wild-type (Solanum lycopersicum cv. Ailsa Craig) and ABA-deficient mutant (notabilis) seedlings were used to determine the membrane lipid peroxidation, osmotic substance and chlorophyll contents. ABA synthesis and signal transduction changes and ABA roles regulating the antioxidation in tomato seedlings subject to saline-alkaline stress were further explored. Results showed that ABA synthesis and signal transduction were induced by saline-alkaline stress. Under saline-alkaline stress, tomato seedlings had decreased relative water content, increased relative electrical conductivity and malondialdehyde content, and these changes were alleviated by exogenous ABA treatment. Exogenous ABA alleviated the degradation of chlorophyll in the leaves of tomato seedlings caused by saline-alkaline stress, further promoted the accumulation of proline and soluble sugar, reduced the content of ROS and improved the ability of the antioxidant enzyme system. Moreover, notabilis appeared to be sensitive to saline-alkaline stress. Overall, ABA is involved in the resistance of tomato seedlings to saline-alkaline stress, and exogenous ABA improves the saline-alkaline tolerance of tomato seedlings.

RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity-alkalinity stress.

Salinity-alkalinity stress is a limiting factor in tomato production in the world. Plants perceive salinity-alkalinity stress by activating signaling pathways to increase plant tolerance (Xu et al., 2020). Here, we investigated whether spermine (Spm) induces respiratory burst oxidase homolog 1 (RBOH1) and hydrogen peroxide (H2O2) signaling in response to salinity-alkalinity stress in tomato. The results showed that exogenous Spm induced the expression of RBOH1 and the accumulation of H2O2 under normal condition. Accordingly, we tested the function of H2O2 signal in tomato seedlings and found that exogenous H2O2 increased the expression levels of Cu/Zn-superoxide dismutase (Cu/Zn-SOD), catalase 1 (CAT1), cytosolic ascorbate peroxidase (cAPX), and glutathione reductase 1 (GR1) and the activities of SOD (EC 1.15.1.1), CAT (EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and GR (EC 1.6.4.2) in tomato seedlings under salinity-alkalinity stress. DMTU increased the malondialdehyde (MDA) content and relative electrical conductivity, and the relative water content (RWC), and accelerated leaf yellowing in tomato seedlings under salinity-alkalinity stress, even though we sprayed Spm on tomato leaves. We also found that RBOH1 silencing decreased the expression levels of Cu/Zn-SOD, CAT1, cAPX, and GR1 and the activities of SOD, CAT, APX, and GR when tomato seedlings were under salinity-alkalinity stress. Exogenous Spm did not increase RWC and decrease MDA content in RBOH1 silencing tomato seedlings under salinity-alkalinity stress.