Downregulation of paraoxonase 3 contributes to aggressive human hepatocellular carcinoma progression and associates with poor prognosis.

Paraoxonase (PON) enzymes possess antioxidant properties and protect against cardiovascular diseases. As a member of PON family, PON3 is primarily synthesized in the liver and poorly investigated. This study aimed to examine the expression of PON3 in human hepatocellular carcinoma (HCC) and investigate the clinical significance and biological function of PON3 in HCC patients. We first analyzed PON3 expression in 50 paired HCC samples (HCC tissues vs matched para-cancerous tissues) and 160 clinical HCC specimens by using immunohistochemistry (IHC). Our results showed that the expression of PON3 was downregulated in HCC and significantly associated with tumor-node-metastasis (TNM) stage, tumor size, and tumor number. Kaplan-Meier survival and Cox regression analyses showed that PON3 was an independent prognostic factor for overall survival (OS) and time to recurrence (TTR). Finally, we aimed to reveal the biological function of PON3 in HCC growth and metastasis, and our results showed that overexpression of PON3 potently inhibited growth and metastasis of HCC. Collectively, our study demonstrated that PON3 exhibited tumor-suppressive effects toward HCC and it might serve as a novel prognostic marker in HCC.

Harvesting Energy from Atmospheric Water: Grand Challenges in Continuous Electricity Generation.

Atmospheric water is ubiquitous on earth and extensively participates in the natural water cycle through evaporation and condensation. This process involves tremendous energy exchange with the environment, but very little of the energy has so far been harnessed. The recently emerged hydrovoltaic technology, especially moisture-induced electricity, shows great potential in harvesting energy from atmospheric water and gives birth to moisture energy harvesting devices. The device performance, especially the long-term operational capacity, has been significantly enhanced over the past few years. Further development; however, requires in-depth understanding of mechanisms, innovative materials, and ingenious system designs. In this review, beginning with describing the basic properties of water, the key aspects of the water-hygroscopic material interactions and mechanisms of power generation are discussed. The current material systems and advances in promising material development are then summarized. Aiming at the chief bottlenecks of limited operational time, advanced system designs that are helpful to improve device performance are listed. Especially, the synergistic effect of moisture adsorption and water evaporation on material and system levels to accomplish sustained electricity generation is discussed. Last, the remaining challenges are analyzed and future directions for developing this promising technology are suggested.

The reference genome of Miscanthus floridulus illuminates the evolution of Saccharinae.

Miscanthus, a member of the Saccharinae subtribe that includes sorghum and sugarcane, has been widely studied as a feedstock for cellulosic biofuel production. Here, we report the sequencing and assembly of the Miscanthus floridulus genome by the integration of PacBio sequencing and Hi-C mapping, resulting in a chromosome-scale, high-quality reference genome of the genus Miscanthus. Comparisons among Saccharinae genomes suggest that Sorghum split first from the common ancestor of Saccharum and Miscanthus, which subsequently diverged from each other, with two successive whole-genome duplication events occurring independently in the Saccharum genus and one whole-genome duplication occurring in the Miscanthus genus. Fusion of two chromosomes occurred during rediploidization in M. floridulus and no significant subgenome dominance was observed. A survey of cellulose synthases (CesA) in M. floridulus revealed quite high expression of most CesA genes in growing stems, which is in agreement with the high cellulose content of this species. Resequencing and comparisons of 75 Miscanthus accessions suggest that M. lutarioriparius is genetically close to M. sacchariflorus and that M. floridulus is more distantly related to other species and is more genetically diverse. This study provides a valuable genomic resource for molecular breeding and improvement of Miscanthus and Saccharinae crops.

[Bibliometrics evaluation of biological feed based on patents].

As one of the emerging industries, feed industry not only effectively utilizes existing agricultural resources but also provides a strong material foundation and protection for animal food. In this article, status and trends of biological feed in the world based on bibliometrics were analyzed. Issues including major institutions, international cooperation, status, trends and frontiers were analyzed. These co-countries map and keywords clustering analysis can reveal co-countries and hot spots in this field. These analyses can help the researchers get an overview of this field quickly and accurately.

A stretchable, self-healing conductive hydrogels based on nanocellulose supported graphene towards wearable monitoring of human motion.

Stretchable, self-healing and conductive hydrogels have attracted much attention for wearable strain sensors, which are highly required in health monitoring, human-machine interaction and robotics. However, the integration of high stretchability, self-healing capacity and enhanced mechanical performance into one single conductive hydrogel is still challenging. In this work, a type of stretchable, self-healing and conductive composite hydrogels are fabricated by uniformly dispersing TEMPO-oxidized cellulose nanofibers (TOCNFs)-graphene (GN) nanocomposites into polyacrylic acid (PAA) hydrogel through an in-situ free radical polymerization. The resulting hydrogels demonstrate a stretchability (∼850 %), viscoelasticity (storage modulus of 32 kPa), mechanical strength (compression strength of 2.54 MPa, tensile strength of 0.32 MPa), electrical conductivity (∼ 2.5 S m-1) and healing efficiency of 96.7 % within 12 h. The hydrogel-based strain sensor shows a high sensitivity with a gauge factor of 5.8, showing great potential in the field of self-healing wearable electronics.

Highly Stretchable and Self-Healing Strain Sensors Based on Nanocellulose-Supported Graphene Dispersed in Electro-Conductive Hydrogels.

Intrinsic self-healing and highly stretchable electro-conductive hydrogels demonstrate wide-ranging utilization in intelligent electronic skin. Herein, we propose a new class of strain sensors prepared by cellulose nanofibers (CNFs) and graphene (GN) co-incorporated poly (vinyl alcohol)-borax (GN-CNF@PVA) hydrogel. The borax can reversibly and dynamically associate with poly (vinyl alcohol) (PVA) and GN-CNF nanocomplexes as a cross-linking agent, providing a tough and flexible network with the hydrogels. CNFs act as a bio-template and dispersant to support GN to create homogeneous GN-CNF aqueous dispersion, endowing the GN-CNF@PVA gels with promoted mechanical flexibility, strength and good conductivity. The resulting composite gels have high stretchability (break-up elongation up to 1000%), excellent viscoelasticity (storage modulus up to 3.7 kPa), rapid self-healing ability (20 s) and high healing efficiency (97.7 ± 1.2%). Due to effective electric pathways provided by GN-CNF nanocomplexes, the strain sensors integrated by GN-CNF@PVA hydrogel with good responsiveness, stability and repeatability can efficiently identify and monitor the various human motions with the gauge factor (GF) of about 3.8, showing promising applications in the field of wearable sensing devices.