RESUMO
Four peptide sequences characterized by high content of hydrophobic, charged, and polar amino acids were obtained from 23 clones of M13 phage. Peptides P2 and P4 exhibited highest binding affinity for immobilized trypomastigotes. The inhibitory effects of peptides seemed to be due to blockade of certain epitopes on T. cruzi surface proteins responsible for interactions with the respective receptors of host cells.
Assuntos
Estágios do Ciclo de Vida/efeitos dos fármacos , Oligopeptídeos/farmacologia , Biblioteca de Peptídeos , Tripanossomicidas/farmacologia , Trypanosoma cruzi/efeitos dos fármacos , Sequência de Aminoácidos , Sítios de Ligação , Biotinilação , Células HeLa , Humanos , Estágios do Ciclo de Vida/fisiologia , Oligopeptídeos/metabolismo , Ligação Proteica , Tripanossomicidas/metabolismo , Trypanosoma cruzi/crescimento & desenvolvimento , Trypanosoma cruzi/metabolismoRESUMO
Experiments with passive transfer of immunocompetent cell and serum demonstrated suppression of tumor growth after transfer of splenocytes from animals immunized with Trypanosoma cruzi. The nonspecific constituent of antitumor effect of immunocompetent cells was detected. The previously reported oncoprotective effect of mucins 2 and 3 was not confirmed.