RESUMO
Objective: To investigate the correlation of cognitive dysfunction with intracranial lesions and symptoms of depression and anxiety in patients with neuromyelitis optica spectrum disorders (NMOSD). Methods: Thirty-one NMOSD patients (7/24 males/females) were enrolled in the Department of Neurology of the Sixth Medical Center of the PLA General Hospital from August 2019 to August 2022. The average age was 42±13 years, and the average education level was 12 (9, 12) years. There were 30 healthy controls, 11/19 males/females, with an average age of 47±9 years and an average education of 12 (9, 15) years. The general clinical data and imaging data were collected, and the subjects were assessed on their cognition, anxiety and depression using the assessment scale approved at home and abroad. A cross-sectional study was conducted on them. The t-test or Wilcoxon test was used for inter-group comparison, and Pearson test or Spearman test was used to explore the correlation between the cognition of NMOSD patients and their intracranial lesions, depression and anxiety. Results: Compared with the healthy control group, NMOSD patients had significantly lower scores on MoCA (Z=-3.10,P=0.002), CRAVLT-N7 (Z=-5.12, P<0.001), CRAVLT-N8 (t=-4.40, P<0.001), ROCF-R (t=-3.10,P<0.01), ROCF-C (Z=-2.72,P<0.01), PASAT-3 (Z=-2.71,P<0.01), PASAT-2 (Z=-3.14,P<0.01), and CWT-A (Z=-3.10,P<0.01)scales. Frontal lobe lesions were negatively correlated with PASAT-2 (r=-0.448, P=0.012) scores, temporal lobe lesions were negatively correlated with CRAVLT-N9 (r=-0.564, P=0.001), and parietal lobe lesions were negatively correlated with MoCA (r=-0.374, P=0.038), PASAT-3 (r=-0.426, P=0.017), and PASAT-2 (r=-0.459, P=0.009) scores; The scores of MoCA (r=-0.392, P=0.029), CRAVLT-N6 (r=-0.396, P=0.028), CRAVLT-N7 (r=-0.415, P=0.020), CRAVLT-N8 (r=-0.406, P=0.023), PASAT-3 (r=-0.537, P=0.002) and PASAT-2 (r=-0.495, P=0.005) scales were negatively correlated with the scores of HAMD assessment, and the scores of PASAT-3 (r=-0.499, P=0.004) and PASAT-2 (r=-0.452, P=0.011) were negatively correlated with the scores of HAMA. Conclusions: The cognitive function of patients with NMOSD is significantly reduced, involving multiple cognitive domains. The cognitive function is affected by the distribution of intracranial lesions and the degree of depression and anxiety.
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Neuromielite Óptica , Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Neuromielite Óptica/patologia , Depressão , Estudos Transversais , Cognição , AnsiedadeRESUMO
Objective: To investigate the collateral circulation compensation model in patients with favorable prognosis of basilar artery occlusion/severe stenosis treated with drugs or endovascular therapy. Methods: Clinical data of patients with basilar artery occlusion/severe stenosis and good clinical outcome were retrospectively collected in the Department of Neurology, Sixth Medical Center of PLA General Hospital from January 2019 to January 2020. They were divided into intensive drug therapy group and combined endovascular therapy group. The number and ways of collateral compensation pathway described by digital substraction angiography (DSA) were analyzed, and the characteristics of the collateral compensation model were summarized. SPSS22.0 software was used for statistical analysis, and the constituent ratio (%) was used for statistical description of the enumeration data. Results: A total of 32 eligible patients were included, including 27 males and 5 females, with an average age 45-76 (59±10) years. The compensation model included posterior communicating artery-posterior cerebral artery (13 cases, 40.6%), posterior communicating artery-posterior cerebral artery-basilar artery (10 cases, 31.2%), cerebellar artery-anastomotic branches of superior cerebellar artery (8 cases, 25.0%), anterior choroid artery-anastomotic branches of posterior choroid artery (2 cases, 6.2%), collateral circulation not established (11 cases, 34.4%).In drug treatment group, collateral compensation was found in the majority (14/15), with mainly posterior communicating artery (10/14).Most patients in combined treatment group did not develop collateral compensation (10/17), anastomotic branches of PICA-SCA were the main routes (6/7). Conclusion: In patients with basilar artery occlusion/severe stenosis, favorable clinical outcome can be achieved in both groups of patients treated with intensive drug therapy or endovascular therapy.
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Artéria Basilar , Insuficiência Vertebrobasilar , Idoso , Artéria Basilar/diagnóstico por imagem , Circulação Cerebrovascular , Circulação Colateral , Constrição Patológica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Insuficiência Vertebrobasilar/diagnóstico por imagem , Insuficiência Vertebrobasilar/tratamento farmacológicoRESUMO
The clinical data of 47 patients [41 males, 6 females, aged (62±7) years] treated at the Department of Neurology of the Sixth Medical Center of PLA General Hospital from October 2018 to October 2020 who were diagnosed with severe bilateral vertebral artery stenosis/occlusion through Digital Subtraction Angiography (DSA) were retrospectively collected, and the characteristics of the compensatory pathways of collateral circulation were determined. Results showed that there were 8 intracranial and extracranial compensatory pathways, with posterior communicating artery as the major type (21 cases) for intracranial ones and deep carotid artery as the major type (16 cases) for extracranial ones. As the establishment of the compensatory pathway is closely related to the lesion site, DSA assessment for patients with severe bilateral vertebral artery stenosis/occlusion is helpful for clinicians to make judgement on prognosis of patients and choose individualized treatment plans.
Assuntos
Estenose das Carótidas , Insuficiência Vertebrobasilar , Angiografia Digital , Artéria Carótida Interna , Circulação Cerebrovascular , Circulação Colateral , Tontura/etiologia , Feminino , Humanos , Masculino , Estudos Retrospectivos , Artéria Vertebral/diagnóstico por imagemRESUMO
Objective: To explore the predictive effect of pelvic floor muscle function on stress urinary incontinence (SUI) . Methods: A total of 258 women in gynecological outpatients at Fuzhou General Hospital were evaluated the pelvic floor muscle function by intravaginal manometery, then all of outpatients were divided into urinary incontinence group and non-incontinence group, and compared pelvic floor muscle function and clinical characteristic to establish prediction model of SUI by classification tree and analyse the predictive role of pelvic floor muscle function for SUI. Results: There were significant difference in body mass index [BMI; (22.8±2.9) vs (21.5±2.7) kg/m2, P<0.05], maximum newborn weight [ (3 396±424) vs (3 284±384) g, P<0.05] between urinary incontinence group (n=114) and non-incontinence group (n=144) . However, there were no significant differences in age, parity and mode of delivery between two groups (all P>0.05) . There were significant differences (all P<0.01) in maximum vaginal pressure [ (21±7) vs (35±9) mmHg (1 mmHg=0.133 kPa) ], average pressure [ (13±7) vs (23±9) mmHg], fatigue [ (-65±20) % vs (-46±17) %] and collecting time [ (1.0±0.6) vs (0.8±0.5) s] between two groups. Prediction model, which obtained by classification tree analysis with the affecting factors of SUI (including BMI, maximum vaginal pressure, fatigue and collecting time) , suggested that the incidence of SUI was 88.6% (70/79) , when maximum vaginal pressure ≤26.2 mmHg. While, when maximum vaginal pressure was greater than 28.2 mmHg, there was no occurrence of SUI (0/7) . But it would increase, when BMI >22.6 kg/m2. Conclusions: The occurrence of SUI is related to the BMI and pelvic floor muscles function. It would increase the risk of SUI with vaginal maximum pressure (≤26.2 mmHg) and BMI (>22.6 kg/m2) . While there is almost no SUI, while vaginal maximum pressure >28.2 mmHg. To select high-risk group of SUI and intervene early according to the prediction model, which may be make sense of reducing incidence of SUI.
Assuntos
Incontinência Urinária por Estresse , Feminino , Humanos , Recém-Nascido , Músculo Esquelético , Paridade , Diafragma da Pelve , Gravidez , VaginaRESUMO
Objective: To evaluate transperineal sonography for lower urinary tract symptoms after pelvic floor reconstruction. Methods: Eighty-three patients with severe pelvic organ prolapse received surgeries in Fuzhou General Hospital from September 2014 to September 2015, dividing into two groups: 27 patients were selected to receive transvaginal mesh (TVM) pelvic floor reconstruction surgery with tension-free vaginal tape-Abbrevo (TVT-Abbrevo) incontinence surgery, named TVM+TVT-Abbrevo group; 56 patients were selected to receive TVM pelvic floor reconstruction surgery only, named TVM group. The ultrasonic parameters at rest, on contraction and Valsalva condition respectively were observed and measured, including the bladder neck descent (BND), urethral rotation angle, retrovesical angle, levator urethra gap (LUG), the existence of bladder neck funneling, position of the tape, by using 2D and 3D transperineal ultrasound. Results: The two groups were compared with the ultrasonic parameters before and after operation: two groups of patients with postoperative BND [(2.3±0.5) versus (3.1±0.7) cm, (1.6±0.4) versus (3.6±0.4) cm] were significantly reduced, the difference was statistically significant (P=0.02, P<0.01). The two groups of LUG before and after operation [(3.62±0.45) versus (3.26±0.92) cm, (2.96±0.47) versus (2.72±0.38) cm] both had significant difference by maximum Valsalva (P<0.01, P=0.04). There was statistical significance difference of urethral rotation angle in TVM+TVT-Abbrevo group by maximum Valsalva (P=0.01). Observation of morphology: (1) 2 patients with difficulty in urination in TVM+TVT-Abbrevo group, ultrasound showed when the position of the bladder down the urethra discount; 4 patients with stress urinary incontinence (SUI), ultrasound showed slings off or release. (2) One patient with difficulty in urination in TVM group, but ultrasound showed lower urinary tract anatomy were normal; 5 patients with SUI, ultrasound showed the position of the bladder neck were significantly lower in 3 patients, showing high mobility, and the other 2 patients had a larger urethral diameter, showing a tendency of natural deletion. Conclusion: s Anatomy of lower urinary tract could be clearly showed by transperineal sonography. This could provide imaging support for the diagnosis of lower urinary tract symptoms after pelvic floor reconstruction.
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Sintomas do Trato Urinário Inferior/diagnóstico por imagem , Diafragma da Pelve/cirurgia , Prolapso de Órgão Pélvico/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Ultrassonografia/métodos , Feminino , Humanos , Pessoa de Meia-Idade , Período Pós-Operatório , Slings Suburetrais/efeitos adversos , Resultado do Tratamento , Incontinência Urinária por Estresse/epidemiologiaRESUMO
OBJECTIVE: To seek the predictive value of pudendal nerve function that need preventive anti-incontinence surgery at the same time following pelvic prolapse surgery in severe pelvic organ prolapse (POP) patients. METHODS: Seventy women completed this study from January 2014 to June 2015 in Fuzhou General Hospital of Nanjing Military Command, dividing into four groups: POP with or without coexisting occult stress urinary incontinence (OSUI) in preoperation, women with persistent stress urinary incontinence (SUI) in postoperation, women without SUI in postoperation. The pudendal nerve function in preoperation was measured by using Solar Urodynamic Neuro Module, including pudendal nerve terminal motor latency (PNTML), and amplitude. RESULTS: There were statistical significance on bilateral PNTML between POP coexisting OSUI group and only severe POP group [(2.62±0.23) versus (2.40±0.26) ms in right of PNTML, (2.55± 0.21) versus (2.37 ±0.30) ms in left of PNTML; all P<0.05], but no statistical significance on bilateral amplitude (P>0.05). Compared de novo SUI group with POP group in postoperation, de novo SUI group's right of PNTML was significantly increased [(2.74±0.16) versus (2.47±0.26) ms; P< 0.05]; and the right of PNTML was extending 2.5 standard deviation at least compared with the health's [(2.10±0.20) ms]. CONCLUSIONS: The PNTML of pudendal nerve of POP coexisting OSUI is severe than only severe POP, the velocity of nerve conduction is slowing, and PNTML extension has a predictive value for postoperative urinary incontinence. When the right of PNTML of preoperative POP increased by at least 2.5 standard deviations than health's, the risk of SUI postoperative strongly increased, and a anti-incontinence surgery at the same time following pelvic prolapse surgery should be adviced.
Assuntos
Prolapso de Órgão Pélvico/cirurgia , Nervo Pudendo , Incontinência Urinária por Estresse/diagnóstico , Incontinência Urinária por Estresse/fisiopatologia , Potenciais Evocados/fisiologia , Feminino , Humanos , Prolapso de Órgão Pélvico/complicações , Prolapso de Órgão Pélvico/fisiopatologia , Complicações Pós-Operatórias , Período Pós-Operatório , Nervo Pudendo/fisiologia , Resultado do Tratamento , Incontinência Urinária por Estresse/etiologia , Urodinâmica , Prolapso Uterino/fisiopatologiaRESUMO
We aimed to detect expressional profiles of intracellular cell adhesion molecule-1 (ICAM-1), nuclear factor-kappa B (NF-κB), and monocyte chemoattractant protein-1 (MCP-1) in human cerebral aneurysm, in order to investigate the effect of chronic inflammation on the pathogenesis of cerebral aneurysm. Samples from 40 cases of human cerebral aneurysms diagnosed at our hospital were selected along with 20 normal cerebral artery samples. Western blotting and immunohistochemical (IHC) staining were used to reveal expressional profiles of ICAM-1 and NF-κB in the aneurysmal wall of patients and normal cerebral artery tissues. Reverse transcription (RT)-PCR was employed to detect changes in transcript levels of MCP-1 mRNA. Western blotting showed significantly higher expressions of ICAM-1 and NF-κB in patients with cerebral aneurysm compared to the normal group (P < 0.01), which was consistent with IHC staining results. RT-PCR revealed significantly higher MCP-1 transcripts in cerebral aneurysm tissues compared to the normal group (P < 0.01), in addition to a positive relationship between ICAM-1 and NF-κB expression levels. In conclusion, expression levels of ICAM-1, NF-κB, and MCP-1 in patients are significantly elevated, suggesting an enhanced chronic inflammatory response and a significant correlation between inflammatory factors/adhesion molecules and the pathogenesis of cerebral aneurysm.
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Quimiocina CCL2/biossíntese , Inflamação/genética , Molécula 1 de Adesão Intercelular/biossíntese , Aneurisma Intracraniano/genética , NF-kappa B/biossíntese , Adulto , Artérias Cerebrais/patologia , Quimiocina CCL2/genética , Feminino , Regulação da Expressão Gênica , Humanos , Inflamação/patologia , Molécula 1 de Adesão Intercelular/genética , Aneurisma Intracraniano/patologia , Masculino , Pessoa de Meia-Idade , NF-kappa B/genética , RNA Mensageiro/biossínteseRESUMO
The aim of this study was to investigate the roles of Fas/FasL, Bcl-2/Bax, and Caspase-8 mRNA expressions in nonalcoholic fatty liver disease (NAFLD). The apoptosis percentage was measured by flow cytometry, the immunohistochemical assay was performed for the determination of Fas, FasL, Bcl-2, and Bax expressions, and a real-time polymerase chain reaction (PCR) assay was performed to detect Caspase-8 mRNA expression. Flow cytometry showed that the apoptosis percentage of the rat liver in the experimental group increased, which increased more obviously with the extension of modeling time. Immunohistochemistry showed that with increasing hepatic steatosis, Fas and FasL protein staining intensified and the number of positive cells increased; the number of positive cells for Bcl-2 and Bax gradually increased on the 4th, 8th, and 12th weeks in the experimental group, whereas the Bcl-2/Bax ratio decreased. The real-time PCR assay showed that Caspase-8 mRNA expression increased with increasing hepatic steatosis and inflammation, exhibiting a progressively rising trend. Hepatocyte apoptosis could promote NAFLD progression; Fas, FasL, and Caspase-8 mRNA activation were important contributing factors to NAFLD. The upregulation of Bax and Bcl-2 expression might be one important mechanism of the apoptosis in NAFLD.
Assuntos
Proteínas Reguladoras de Apoptose/biossíntese , Caspase 8/biossíntese , Proteína Ligante Fas/biossíntese , Hepatopatia Gordurosa não Alcoólica/genética , Proteínas Proto-Oncogênicas c-bcl-2/biossíntese , Proteína X Associada a bcl-2/biossíntese , Animais , Apoptose/genética , Proteínas Reguladoras de Apoptose/genética , Caspase 8/genética , Proteína Ligante Fas/genética , Citometria de Fluxo , Regulação da Expressão Gênica , Hepatócitos/metabolismo , Hepatócitos/patologia , Humanos , Hepatopatia Gordurosa não Alcoólica/fisiopatologia , Proteínas Proto-Oncogênicas c-bcl-2/genética , RNA Mensageiro/genética , Ratos , Proteína X Associada a bcl-2/genéticaRESUMO
Objective: To investigate the relationship between nutritional risk status and clinical outcome in children with tuberculous meningitis (TBM). Methods: The clinical data (basic information, clinical symptoms and laboratory test results) of 112 patients with TBM, who were admitted to Department of Pediatric Infectious Diseases of West China Second Hospital of Sichuan University,from January 2013 to December 2020 were retrospectively analyzed. The patients were divided into the nutritional risk group and the non-nutritional risk group according to the assessment of the nutritional risk by the STRONGkids Scale. The variables of basic information, clinical symptoms and laboratory test measurements etc. were compared between the two groups by using Student t test, Rank sum test or Chi-square test. Multivariate Logistic regression analysis were used to analyze nutritional risk factors. Results: Among 112 patient with TBM, 55 were males and 57 females. There were 62 cases in the nutritional risk group and 50 cases in the non-nutritional risk group. The proportion of cases with nutritional risk was 55.4% (62/112). Patients in the nutritional risk who lived in rural areas, had symptoms of brain nerve damage, convulsions, emaciation and anorexia, with a diagnosis time of ≥21 days, and the level of cerebrospinal fluid (CSF) protein were all higher than those in the non-nutritional risk group ((50 cases (80.6%) vs. 32 cases (64.0%), 20 cases (32.3%) vs.8 cases (16.0%), 33 cases (53.2%) vs. 15 cases (30.0%), 30 cases (48.4%) vs. 2 cases (4.0%), 59 cases (95.2%) vs. 1 case (2.0%),41 cases (66.1%) vs.18 cases (36.0%), 1 406 (1 079, 2 068) vs. 929 (683, 1 208) mg/L, χ2=3.91, 3.90, 6.10, 26.72, 98.58, 10.08, Z=4.35, all P<0.05). The levels of serum albumin,hemoglobin,lymphocyte count, white blood cell count, and CSF glucose were significantly lower in patients with nutritional risk ((36±5) vs. (41±4) g/L, (110±17) vs. (122±14) g/L, 1.4 (1.0, 2.0)vs. 2.3 (1.6, 3.8)×109/L, 7.8 (6.3, 10.0)×109 vs. 10.0 (8.3, 12.8)×109/L, 1.0 (0.8, 1.6) vs. 2.1 (1.3, 2.5) mmol/L, t=-6.15, -4.22, Z=-4.86, -3.92, -4.16, all P<0.05).Increased levels of serum albumin (OR=0.812, 95%CI:0.705-0.935, P=0.004) and lymphocyte count (OR=0.609, 95%CI:0.383-0.970, P=0.037) may reduce the nutritional risk of children with TBM; while convulsions (OR=3.853, 95%CI:1.116-13.308, P=0.033) and increased level of CSF protein (OR=1.001,95%CI:1.000-1.002, P=0.015) may increase the nutritional risk of children with TBM. Similarly, the rate of complications and drug-induced liver injury was higher in the nutritional risk group (47 cases (75.8%) vs. 15 cases(30.0%), 31 cases (50.0%) vs.8 cases (16.0%), χ2=23.50, 14.10, all P<0.05). Moreover, the length of hospital stay was also longer in the nutritional risk group ((27±13) vs. (18±7) d, t=4.38, P<0.05). Conclusions: Children with TBM have a high incidence of nutritional risk. Convulsive, the level of serum albumin, the level of lymphocyte count and CSF protein may affect the nutritional risk of children with TBM. The nutritional risk group has a high incidence of complications and heavy economic burden.It is necessary to carry out nutritional screening and nutritional support for children with TBM as early as possible.
Assuntos
Tuberculose Meníngea , Feminino , Humanos , Contagem de Leucócitos , Masculino , Avaliação Nutricional , Estado Nutricional , Estudos Retrospectivos , Tuberculose Meníngea/líquido cefalorraquidiano , Tuberculose Meníngea/diagnósticoRESUMO
OBJECTIVE: Bladder cancer is the most frequent tumor of the urinary system. Despite variety of new treatment options, bladder cancer remains a main global medical problem. Our purpose was to explore the potential molecular and therapeutic targets of bladder cancer diagnosis. PATIENTS AND METHODS: The qRT-PCR was used to assess the expression of miR-20a in tissues and cell lines. Counting Cell Kit-8 (CCK-8) assay was carried out to evaluate cell proliferation. Cell migration was calculated using the transwell assay. RESULTS: The expression of miR-20a increased and PDCD4 decreased in bladder cancer tissues compared with normal tissues. Overexpression of miR-20a promoted T24 cell proliferation and migration, while miR-20a inhibitor suppressed cell proliferation and migration. MiR-20a targeted PDCD4 to regulate its expression in T24 cells. MiR-20a is inversely related to PDCD4 and PTENPL in bladder cancer tissues. Upregulation of PDCD4 suppressed T24 cell proliferation and migration. CONCLUSIONS: The PTENP1/miR-20a/PTEN axis was involved in the progression of bladder cancer. Our study investigated the function of miR-20a in bladder cancer and provided new insights into the treatment of bladder cancer.
Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , MicroRNAs/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , RNA Longo não Codificante/metabolismo , Proteínas de Ligação a RNA/metabolismo , Neoplasias da Bexiga Urinária/metabolismo , Proteínas Reguladoras de Apoptose/genética , Movimento Celular , Proliferação de Células , Células Cultivadas , Humanos , MicroRNAs/genética , PTEN Fosfo-Hidrolase/genética , RNA Longo não Codificante/genética , Proteínas de Ligação a RNA/genética , Neoplasias da Bexiga Urinária/patologiaRESUMO
The electrocaloric and elastocaloric properties at the 180° domain wall in the tetragonal BaTiO3 are studied using the Landau-Ginzburg-Devonshire model as a function of the domain wall rotation angle α. The Ising-Bloch character is predicted at the 180° domain wall in tetragonal BaTiO3 under the flexoelectric effect. The electric field-induced adiabatic temperature change (ΔT E) which is induced by the Bloch-type polarization component depends on α, and a giant positive ΔT E appears at α = (π + 12n)/24 where n is an integer. The asymmetry of ΔT E is found around the Bloch-type domain wall. The Bloch-type polarization component has a little contribution to the stress-induced adiabatic temperature change. This calculation indicates a contribution of helix polarization at the domain wall on the caloric effects (CEs) in the ferroelectric materials.
RESUMO
Electrocaloric effect (ECE) has the potential applications in solid-state refrigeration with the features of high efficiency and environmentally friendly. Large adiabatic temperature change in a wide temperature range is needed for electrocaloric effect to meet the requirement of commercially application. In this work, giant electrocaloric effect is found in PbTiO3 nanoparticle with double-vortex domain structure in a wide temperature range by using phase field method, which the lowest and highest adiabatic temperature change (ΔT) is 7.2 K and 16.5 K, respectively. The influence of misfit strain on the ECE of PbTiO3 nanoparticle with the double-vortex domain structure is investigated, and results show that the compress misfit strain can enhance the ECE, but the tensile misfit strain reduces the ECE. This work reveals a way to obtain giant ECE of ferroelectric materials by domain engineering and strain engineering in a wide temperature range.
RESUMO
BACKGROUND: Previous studies suggest that persistent post-surgical pain (PPSP) is correlated with preoperative pain status and amplification of central sensitization. Protein kinase Mζ (PKMζ) is an essential substrate of the late long-term potentiation underlying central sensitization, which is one mechanism of pain memory formation. However, the potential contributions of spinal PKMζ to PPSP, a condition in which preoperative pain is prevalent, are not known. METHODS: Here, a modified 'hyperalgesia priming' model was established to simulate the clinical situation. This model used intraplantar injections of carrageenan (Car) as priming stimuli to elicit persistent nociceptive sensitization after plantar incision in rats. Upon treatment with PKMζ inhibitor ZIP, Scr-ZIP or protein kinase Cs (PKCs) inhibitor NPC-15437, altered behaviour and spinal PKMζ/PKCs expression were observed. RESULTS: A long-lasting hypersensitivity induced by Car-priming was identified and precipitated by subsequent plantar incision in this 'two-hit' paradigm. Post-treatment with ZIP, but not Scr-ZIP and NPC-15437, after the resolution of Car-priming selectively relieved hypersensitivity. In contrast, pre-priming NPC-15437 treatment only prevented Car-induced initial transient hyperalgesia. Immunoassays showed a significant decrease in spinal PKMζ expression after plantar incision with post-priming ZIP treatment as compared with Scr-ZIP and NPC-15437, but no notable differences in PKCs expression were observed. CONCLUSIONS: Spinal PKCs solely contribute to the initial induction of persistent pain, whereas PKMζ plays an essential role in spinal plasticity storage. PKMζ is responsible for the maintenance of peripheral inflammation-primed PPSP. Therefore, spinal PKMζ may be a therapeutic target to prevent surgery-induced chronic pain in patients with preoperative pain.
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Hiperalgesia/tratamento farmacológico , Hiperalgesia/metabolismo , Proteína Quinase C/metabolismo , Inibidores de Proteínas Quinases/uso terapêutico , Animais , Carragenina , Hiperalgesia/induzido quimicamente , Inflamação/metabolismo , Masculino , Medição da Dor , Proteína Quinase C/antagonistas & inibidores , Ratos , Ratos Sprague-DawleyRESUMO
The ultrastructural changes of chronic cardiopathy in diabetic biobreeding rats were observed electronmicroscopically. The thickness of the myocardial capillary basement membrane (CBM) and volume fraction of myofilaments in the myocardium were quantitatively measured with a video interactive display system III (VIDS-III). The effect of metabolic control on chronic diabetic cardiopathy was also investigated.
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Cardiomiopatias/patologia , Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Tipo 1/patologia , Miocárdio/ultraestrutura , Citoesqueleto de Actina/ultraestrutura , Animais , Angiopatias Diabéticas/patologia , Ratos , Ratos Endogâmicos BBRESUMO
Microscopic quantitative cardiomyopathy was previously reported in spontaneous diabetic biobreeding (BB) rats, but the nature of the lesions remains obscure. To further study the specificity of cardiomyopathy in streptozotocin-diabetic rats and discuss the existence of diabetic heart disease, we compared 40 streptozotocin-diabetic rats (D group) with 40 normal control rats (C group) at various durations in plasma glucose, fructosamine, plasma lipid, left ventricular enzyme contents and alterations of myocardial cells and coronary artery tree under electron and light microscope respectively. In 4 weeks, D rats showed myocardial mitochondrial swelling and degeneration, whereas at 8 weeks, myocardial enzyme contents markedly decreased, and myocardial lesions were more conspicuous with disruption of myocardial cells, formation of myocardial contraction bands, dilatation of intercalated discs, deposition of glycogen granules, etc. At 11 weeks, microscopic changes were essentially similar, whereas the decrease of enzyme contents became more conspicuous. During the 11 weeks, persistent and marked hyperglycemia and elevation of fructosamine were observed, however, no abnormalities were found along the coronary artery tree in the D rats. The results indicated that cardiomyopathy is diabetes specific, and exists independently as one of the three important components of diabetic heart disease.
Assuntos
Cardiomiopatias/etiologia , Diabetes Mellitus Experimental/complicações , Miocárdio/patologia , Animais , Cardiomiopatias/patologia , Frutosamina , Hexosaminas/sangue , Mitocôndrias Cardíacas/ultraestrutura , Ratos , Ratos Sprague-DawleyRESUMO
PIP: A series of 146 women taking compound quinestrol (CQ) was tested for glucose tolerance and insulin release to determine the effects of CQ on these parameters. 23 normal subjects (6 male and 17 female) and 12 diabetics (7 male and 5 female) were also tested and their results were compared with those from the CQ-taking women. The curves of glucose tolerance, insulin release, release index during different phases, and the immunoreactive insulin-blood glucose (IRI-BG) distribution were compared. In this series, the insulin release curve, 45-minutes-after-release index, and IRI-BG distribution of subjects on CQ approximated those of normals, but differ dramatically from diabetic subjects. The glucose intolerance which accompanied the more active insulin reaction caused by CQ seems to gradually ameliorate or revert to normal after CQ withdrawal, a phenomenon which does not occur in true diabetics. Hence, the glucose intolerance caused by CQ is due to the drug's effect on the metabolism of carbohydrates or of insulin.^ieng
Assuntos
Glucose/metabolismo , Norpregnatrienos/efeitos adversos , Quinestrol/efeitos adversos , Adulto , Diabetes Mellitus/metabolismo , Feminino , Teste de Tolerância a Glucose , Humanos , Insulina/metabolismo , Secreção de Insulina , Masculino , Pessoa de Meia-IdadeRESUMO
Pre- and postoperative hypothalamic-pituitary-thyroid axis function was studied in 38 patients with pituitary adenomas (PRL, GH and ACTH tumours), of whom 35 were surgically confirmed and three diagnosed by clinical signs, CT scanning and hormone assessments. About ten days after operation, the same study was repeated in 10 patients with prolactinoma and 7 with growth hormone (GH) tumour. The preoperative abnormal serum TSH response to TRH was found in 8/20 patients with prolactinoma, 9/16 with GH tumour, and 2/2 with Cushing's disease due to ACTH microadenoma. The incidence of abnormal TSH response to TRH was not significantly increased in patients with larger adenoma in either PRL or GH tumour group. In 8 cases of prolactinoma, metoclopramide (MCP, 10 mg, P.O.) test was also performed and there was a significant positive correlation between TSH responses to TRH and to MCP. Serum TT3 in the GH tumour group was within normal ranges, but significantly higher than that of the normal and prolactinoma groups. After operation, TT3 was significantly decreased as compared with that before operation and there were marked changes in TSH response to TRH. In conclusion, there were some abnormalities in TSH control in patients with non-TSH pituitary tumour, and in serum TT3 control in patients with GH tumour. The surgical treatment of pituitary adenoma can lead to transient decrease in TSH reserve and serum TT3 level probably resulting from both stress and/or destruction of thyro-trophs by the operation.
Assuntos
Adenoma/fisiopatologia , Hormônio Adrenocorticotrópico/metabolismo , Sistema Hipotálamo-Hipofisário/fisiopatologia , Neoplasias Hipofisárias/fisiopatologia , Prolactinoma/fisiopatologia , Glândula Tireoide/fisiopatologia , Adenoma/cirurgia , Adolescente , Adulto , Feminino , Hormônio do Crescimento/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Hipofisárias/cirurgia , Prolactinoma/cirurgia , Tireotropina/metabolismoRESUMO
Sorbitol pathway activity, myo-inositol content and ouabain-sensitive and ouabain-resistant ATPase activities were measured in homogenates of the sciatic nerve from alloxan-induced diabetic rats for 4, 8 and 12 weeks. Compared with the age-matched control, glucose, fructose and sorbitol contents of the sciatic nerve from diabetic rats for 4, 8 and 12 weeks were increased respectively to a level of 3-4 fold, 3-5 fold and 6-9 fold of those of the control rats. Myo-inositol was reduced approximately 50%. There was a significant decrease (P less than 0.01) in total ATPase, ouabain-sensitive and ouabain-resistant ATPase activities. These changes could be relevant to the development of diabetic neuropathy.
Assuntos
Diabetes Mellitus Experimental/metabolismo , Neuropatias Diabéticas/metabolismo , Nervo Isquiático/metabolismo , ATPase Trocadora de Sódio-Potássio/metabolismo , Sorbitol/metabolismo , Animais , Glucose/metabolismo , Inositol/metabolismo , Masculino , Ouabaína/metabolismo , Ratos , Ratos EndogâmicosRESUMO
We have synthesized a 162 bp gene of human Plasmodium falciparum hybrid peptide antigen by the solid-phase phosphoramidite method with ABI 381A DNA synthesizer. The gene encodes three fragments of the relative molecules 83 kDa, 55 kDa and 35 kDa merozoite-specific proteins and two CS repeats or four peptides. The gene with the designed two cohesive ends was divided into 8 fragments to be synthesized. All synthetic fragments were annealed and ligated with T4 DNA ligase to form double DNA chain. This synthetic gene was recombined with P-Blue script as vector and transformed into E. coli JM109. The positive recombinants were screened out by dot hybridization and enzyme analysis. The DNA sequence analysis showed that the synthesized human Plasmodium falciparum hybrid peptide antigen gene was identical with the designed one. (Figs. 1-4).