Three new triterpene saponins from Clematis chinensis.

Three new triterpene saponins, clematochinenosides H-J (1-3), were isolated from the roots and rhizomes of Clematis chinensis. Their structures were elucidated on the basis of spectroscopic means including 1D and 2D NMR experiments and hydrolysis products.

Chemical constituents from leaves of Evodia lepta.

To explore the alternative material for the stems of Evodia lepta used in clinic, the leaves extract of E. lepta was chemically investigated by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC and the structures of the compounds were identified mainly by spectroscopic methods. Ten known compounds 4-hydroxy-4, 7-dimethyl-1-tetralone (1), (6R, 7E) -4, 7-megastigmadien-3, 9-dione (2), 4-megastigmen-3, 9-dione (3), formononetin (4), daidzein (5), oroxylin A (6), wogonin (7), 5, 7-dihydroxy-3, 4'-dimethoxyflavone (8), N-trans-coumaroyltyranine (9) and (E) -p-hydroxycinnamic acid (10), have been obtained and identified. All these compounds were isolated from this species for the first time. The results revealed that there is a considerate chemical difference between the stems and leaves of E. lepta.

Prostaglandin-like fatty acid derivatives from Artemisia anomala.

Four prostaglandin-like fatty acid derivatives anomalone A-D (1-4) were isolated from the aerial part of Artemisia anomala S. Moore. Their structures were determined on the basis of extensive spectroscopic analyses.

Inhibitory effect of triterpenoid saponins from the leaves of Ilex kudingcha on aggregated LDL-induced lipid deposition in macrophages.

We have investigated the inhibitory effect of triterpenoid saponins from the leaves of Ilex kudingcha C. J. Tseng on aggregated low-density lipoprotein (LDL)-induced lipid deposition in macrophages. A cell-based screening model was initially applied on aggregated LDL (aggLDL)-induced lipid deposition on macrophages to test the inhibitory effects of the 12 triterpenoid saponins from this plant. Eight of these compounds inhibited the formation of foam cells and reduced intracellular total cholesterol and triglyceride contents. Structure-activity relationship analysis showed the essential role of the delta-lactone ring for the biological activity. The promoter action of the OH group at the C-12 position, the number of monosaccharides in the sugar chain and the rhamnose at the terminal of the sugar chain is also discussed.

Triterpene saponins from the leaves of Ilex kudingcha.

Three new triterpene saponins, ilekudinosides T-V (1-3), along with six known saponins were isolated from the 70% ethanolic extract of the leaves of Ilex kudingcha. The new saponins were characterized as 3-O-beta-d-glucopyranosyl-(1 --> 3)-[alpha-l-rhamnopyranosyl-(1 --> 2)]-alpha-l-arabinopyranosyl-3beta,19alpha-dihydroxy-urs-12(13)-en-28,20beta-lactone (1), 3-O-beta-d-glucopyranosyl-(1 --> 3)-[alpha-l-rhamnopyranosyl-(1 --> 2)]-alpha-l-arabinopyranosyl-3beta,19alpha-dihydroxy-12-ethoxy-urs-13(18)-ene-28,20beta-lactone (2), 3-O-beta-d-glucopyranosyl-(1 --> 3)-[alpha-l-rhamnopyranosyl-(1 --> 2)]-alpha-l-arabinopyranosyl-3beta,19alpha-dihydroxy-11-oxo-urs-13(18)-ene-28,20beta-lactone (3), respectively. The structures of compounds 1-3 were elucidated on the basis of the chemical and spectroscopic evidence, and the structures of known compounds were identified by comparison of their spectroscopic data with those reported in the literature.

[Studies on triterpenoid constituents in leaf of Ilex pernyi].

OBJECTIVE: To investigate the chemical constituents of Ilex pernyi. METHOD: The chemical constituents were isolated by various column chromatographic methods. The structures were identified by spectral data. RESULT: Eight triterpenoid compounds were isolated and identified as ursolic acid (1), lupeol (2), alpha-amyrin (3), uvaol (4), 3beta-hydroxyurs-11-ene-13beta-olide (5), pomolic acid (6), lup-20 (29)-ene-3beta, 24-diol (7), 3beta, 23-dihydroxy-urs-12-en-28-oic acid (8). CONCLUSION: The eight compounds were obtained from this plant for the first time.

Prenylated benzoic acid derivatives from the stem of Euodia lepta.

Two new prenylated benzoic acid derivatives, leptoic acid A and (+)-S-anodendroic acid (1-2), along with one known compound, 2,2-dimethyl-2H-1-benzopyran-6-carboxylic acid (3) were isolated from the stem of Euodia lepta (spreng.) Merr. Their structures were elucidated on the basis of the chemical and spectroscopic evidence.

Polygalins D-G, four new flavonol glycosides from the aerial parts of Polygala sibirica L. (Polygalaceae).

Four new flavonol glycosides (1-4), polygalins D-G, together with 12 known flavonoids (5-16) were isolated from the aerial parts of Polygala sibirica L. The chemical structures of these compounds were characterised by NMR and ESI-MS spectroscopic data and acid hydrolysis results. This report is a continuous research work on the systematic chemical investigations of plants of the genus Polygala in our laboratory.

A novel sterol sulfate and new oligosaccharide polyester from the aerial parts of Polygala sibirica.

Polygalasterol A (1), a new sterol sulfate, and a new oligosaccharide ester, polygalasibiricose I (2), have been isolated from the n-butanol soluble fraction of the aerial parts of Polygala sibirica L. Their structures were determined to be (24R)-24-sulfo-5a-cholestane-3a,7a,12a,24-tetrol (1) and 3-O-benzoyl-beta-D-fructofuranosyl-(2-->1 )-[beta-D-glucopyranosyl-( 1 --2)]-4-0- {4-0-[3-O-[3-O-benzoyl-(2-O-acetyl)-alpha-L-rhamnopyranosyl-(1-->3 )]-2-O-acetyl-alpha-L-rhamnopyranosyl]-(Z)-p-coumaroyl}-alpha-D-glucopyranoside (2), respectively, by HRESIMS, and 1D and 2D NMR spectroscopic studies.

Triterpenoid glycosides from the leaves of Ilex pernyi.

Ten triterpenoid glycosides, including five new ones (1-5), were isolated from the leaves of Ilex pernyi. The chemical structures of 1-5 were determined on the basis of the chemical and spectroscopic evidence.