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BACKGROUND: Isatidis Radix, the root of Isatis indigotica Fort. (Chinese woad) can produce a variety of efficacious compound with medicinal properties. The tetraploid I. indigotica plants exhibit superior phenotypic traits, such as greater yield, higher bioactive compounds accumulation and enhanced stress tolerance. In this study, a comparative transcriptomic and metabolomic study on Isatidis Radix autotetraploid and its progenitor was performed. RESULTS: Through the targeted metabolic profiling, 283 metabolites were identified in Isatidis Radix, and 70 polyploidization-altered metabolites were obtained. Moreover, the production of lignans was significantly increased post polyploidization, which implied that polyploidization-modulated changes in lignan biosynthesis. Regarding the transcriptomic shift, 2065 differentially expressed genes (DEGs) were identified as being polyploidy-responsive genes, and the polyploidization-altered DEGs were enriched in phenylpropanoid biosynthesis and plant hormone signal transduction. The further integrative analysis of polyploidy-responsive metabolome and transcriptome showed that 1584 DEGs were highly correlated with the 70 polyploidization-altered metabolites, and the transcriptional factors TFs-lignans network highlighted 10 polyploidy-altered TFs and 17 fluctuated phenylpropanoid pathway compounds. CONCLUSIONS: These results collectively indicated that polyploidization contributed to the high content of active compounds in autotetraploid roots, and the gene-lignan pathway network analysis highlighted polyploidy-responsive key functional genes and regulators.
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Isatis , Transcriptoma , Regulação da Expressão Gênica de Plantas , Isatis/genética , Metaboloma , Poliploidia , Metabolismo Secundário/genéticaRESUMO
Pathogenic bacterial infection, especially in the wound, may threaten human health. Developing new antibacterial materials for wound healing is still urgent. Metal nanoclusters have been explored as a novel antibacterial agent. Herein, biomolecule gelatin was chosen as a substrate and functionalized with gold/silver clusters for bacterial killing. Through a simple amidation reaction, gold/silver clusters were successfully conjugated in a gelatin substrate to obtain a Au/Ag@gelatin sponge. The presence of gold/silver clusters modified the porous structure of the gelatin. Thus, the water absorption and water retention of the Au/Ag@gelatin sponge were enhanced. More importantly, the gold/silver clusters show aggregation-enhanced emission and strong reactive oxygen generation, that endow the Au/Ag@gelatin sponge with a good antibacterial property. The good physical performance and favorable bactericidal activity of the Au/Ag@gelatin sponge suggest its potential for application as a wound dressing.
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Antibacterianos/farmacologia , Gelatina/farmacologia , Ouro/química , Prata/química , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Gelatina/química , Nanopartículas Metálicas , Testes de Sensibilidade Microbiana , Porosidade , Pseudomonas aeruginosa/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Propriedades de Superfície , SuínosRESUMO
The reduction in habitat quality (as shown, in part, by the increase in habitat rarity) is an important challenge when protecting the Yunnan snub-nosed monkey. We used the InVEST model to quantitatively analyze the dynamic changes in the habitat of the Yunnan snub-nosed monkey from 1975 to 2022. The results show that in the study period, the degree of habitat degradation increased, with the degradation range at its widest in the south, and the degradation intensity highest in the north, especially along a center "spine" area in the north. Over the latter part of the study period, the habitat quality of most monkey groups improved, which is conducive to the survival and reproduction of the population. However, the habitat quality and monkey populations are still at significant risk. The results provide the basis for formulating the protection of the Yunnan snub-nosed monkey and provide research cases for the protection of other endangered species.
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The habitats of the already endangered Yunnan snub-nosed monkey (Rhinopithecus bieti) are degrading as village economies develop in and around these habitat areas, increasing the depopulation and biodiversity risk of the monkey. The paper aims to show the areas of these monkeys' high-quality habitats that are at highest risk of degradation by continued village development and hence be the focus of conservation efforts. Our analysis leveraged multiple tools, including primary component analysis, the InVEST Habitat-Quality model, and GIS spatial analysis. We enhanced our analysis by looking at habitat quality as it relates to the habitat suitability for the monkey specifically, instead of general habitat quality. We also focused on the impact of the smallest administrative scale in China-the village. These foci produced a clearer picture of the monkeys' and villages' situations, allowing for more targeted discussions on win-win solutions for both the monkeys and the village inhabitants. The results show that the northern habitat for the monkey is currently higher quality than the southern habitat, and correspondingly, the village development in the north is lower than in the south. Hence, we recommend conservation efforts be focused on the northern areas, though we also encourage the southern habitats to be protected from further degradation lest they degrade beyond the point of supporting any monkeys. We encourage developing a strategy that balances ecological protection and economic development in the northern region, a long-term plan for the southern region to reduce human disturbance, increase effective habitat restoration, and improve corridor design.
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Fluorescent conjugated polymers (CPs) have attracted considerable interest in biosensing owing to their high fluorescence, tunable bandgap, and good biocompatibility. Aiming at acquiring the desired optical responses of CPs for bioapplications, it is essential that the CPs bind to biological targets with high efficacy and affinity. However, the efficient binding of CPs is largely driven by their effective interaction with target surfaces. In this Review, we will focus on the different surface interactions that pervade between CPs and biological targets. The multiple surface interactions can lead to changes in spatial conformation and distribution of CPs, which manifest alterable optical properties of CPs based on accumulation of target-directed CPs, Förster resonance energy transfer mechanism, and metal-enhanced fluorescence mechanism. Then, we display diverse bioapplications applying CPs-based surface interactions, such as cell imaging, imaging-guided detection, and photodynamic therapy. Finally, the challenges and future developments to control the efficient attachment of CPs to biological targets are discussed. We expect that the understanding of surface interactions between CPs and biological targets benefits the CPs-based system design and expands their applications in biological detections and therapies.
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Corantes Fluorescentes/metabolismo , Polímeros/metabolismo , Membrana Celular/metabolismo , Corantes Fluorescentes/química , Humanos , Ligação de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Nanopartículas/química , Oligonucleotídeos/metabolismo , Polímeros/química , Proteínas/metabolismo , Eletricidade EstáticaRESUMO
Gold nanocluster (AuNC) decorated hydrogels have attracted considerable attention as versatile biomaterials. To date AuNCs and hydrogels have mainly been mixed as independent components. Here, we report the use of AuNCs as reactive monomers in the polymerization of hydrogels. We used a free radical polymerization to copolymerize AuNCs with acrylamide and N-acryloyl glycinamide to prepare stimuli-responsive smart hydrogels. Multiple CâC bonds were decorated on the surface of the AuNCs as active sites for polymerization. These CâC bonds not only protected the structure of the AuNCs from oxidation by free radicals during polymerization but also covalently connected the AuNCs with the polymer chains. This structure ensured good photothermal performance of the AuNCs while preserving the thermoresponsive hydrogen bonds of polymers. Moreover, the copolymerized AuNCs acted as cross-linkers, which improved the mechanical properties of the hydrogels. These smart hydrogels had good stability, efficient photothermal conversion, and a sensitive thermoresponsive. We examined their potential for capture of MDA-MB-231 cells with hyaluronic acid as target molecules. The captured cells were released under 660 nm irradiation. This process of targeted capture and light-controlled remote release could be repeatedly applied. These results suggest that systems based on AuNCs copolymerized with hydrogels have great potential for biomedical applications.
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Portadores de Fármacos/química , Liberação Controlada de Fármacos , Ouro/química , Hidrogéis/química , Luz , Nanopartículas Metálicas/química , Polimerização , Linhagem Celular Tumoral , Radicais Livres/química , Humanos , Ácido Hialurônico/química , Ácido Hialurônico/metabolismo , Ligação de HidrogênioRESUMO
Fluorescent gold nanoclusters with unique luminescence properties have drawn great attention in bioimaging. The gold nanoclusters with controlled multicolor fluorescence may benefit accurate fluorescence imaging. In this work, gold-nanocluster-engineered spiropyran ligands (AuNC-SP) were reported to achieve controlled dual-color imaging under single excitation. Through switching the Förster resonance energy transfer from the gold nanocluster segment to the open-ring state merocyanine by UV-vis irradiation, AuNC-SP possesses reversibly dual-color fluorescence. After AuNC-SP combined with chitosan to form AuNC-SP@CS nanoparticles, the steric protection of chitosan largely improved the oxidative stability of AuNC-SP upon UV irradiation. Moreover, the AuNC-SP@CS could be internalized within cancer cells and released AuNC-SP that further underwent transportation into the nucleus. Thus, the AuNC-SP@CS exhibits excellent and reversible dual-color fluorescence to label not only the cytoplasm but also the nucleus. We envisage the AuNC-SP@CS as an ideal probe for fine subcellular assays in the cell interior.
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Benzopiranos/química , Materiais Biocompatíveis/química , Ouro/química , Indóis/química , Nanopartículas Metálicas/química , Nitrocompostos/química , Imagem Óptica , Raios Ultravioleta , Materiais Biocompatíveis/síntese química , Transferência Ressonante de Energia de Fluorescência , Humanos , Células MCF-7 , Teste de Materiais , Tamanho da PartículaRESUMO
Phototherapeutic approaches, including photothermal therapy (PTT) and photodynamic therapy (PDT), have become a promising strategy to combat microbial pathogens and tackle the crisis brought about by antibiotic-resistant strains. Herein, porous gold nanoparticles (AuPNs) were synthesized as photothermal agents and loaded with indocyanine green (ICG), a common photosensitizer for PDT, to fabricate a nanosystem presenting near-infrared (NIR) light-triggered synchronous PTT and PDT effects. The AuPNs can not only convert NIR light into heat with a high photothermal conversion efficiency (50.6-68.5%), but also provide a porous structure to facilely load ICG molecules. The adsorption of ICG onto AuPNs was mainly driven by electrostatic and hydrophobic interactions with the surfactant layer of AuPNs, and the aggregate state of ICG significantly enhanced its generation of reactive oxygen species. Moreover, taking advantage of its synergistic PTT and PDT effect, the hybrid nanocomposites displayed a remarkable antibacterial effect to the gram-positive pathogen Staphylococcus aureus (S. aureus) upon 808 nm laser irradiation.
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Antibacterianos/administração & dosagem , Ouro/administração & dosagem , Verde de Indocianina/administração & dosagem , Nanopartículas Metálicas/administração & dosagem , Nanocompostos/administração & dosagem , Fármacos Fotossensibilizantes/administração & dosagem , Fototerapia , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/efeitos da radiação , Ouro/efeitos da radiação , Verde de Indocianina/efeitos da radiação , Luz , Nanopartículas Metálicas/efeitos da radiação , Nanocompostos/efeitos da radiação , Fármacos Fotossensibilizantes/efeitos da radiação , Porosidade , Staphylococcus aureus/crescimento & desenvolvimentoRESUMO
In the presence of K2S2O8 and HOAc, 3-(2-azidoaryl) substituted propargyl alcohols can go through chemoselective oxidative radical cyclizations to give a pool of anthranils based on Meyer-Schuster rearrangement. It's proposed that the cyclizations were triggered exclusively by the direct attack of oxygen radicals on the azides. The weak N-O bonds in anthranils could be easily cleaved in the presence of transition metal catalysts and went through aminations with 2-oxo-2-phenylacetic acid and iodobenzene.
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Human FOXP3+ regulatory T (Treg) cells are central to immune tolerance. However, their heterogeneity and differentiation remain incompletely understood. Here we use single-cell RNA and T cell receptor sequencing to resolve Treg cells from healthy individuals and patients with or without acute graft-versus-host disease (aGVHD) who undergo stem cell transplantation. These analyses, combined with functional assays, separate Treg cells into naïve, activated, and effector stages, and resolve the HLA-DRhi, LIMS1hi, highly suppressive FOXP3hi, and highly proliferative MKI67hi effector subsets. Trajectory analysis assembles Treg subsets into two differentiation paths (I/II) with distinctive phenotypic and functional programs, ending with the FOXP3hi and MKI67hi subsets, respectively. Transcription factors FOXP3 and SUB1 contribute to some Path I and Path II phenotypes, respectively. These FOXP3hi and MKI67hi subsets and two differentiation pathways are conserved in transplanted patients, despite having functional and migratory impairments under aGVHD. These findings expand the understanding of Treg cell heterogeneity and differentiation and provide a single-cell atlas for the dissection of Treg complexity in health and disease.
Assuntos
Diferenciação Celular/genética , Fatores de Transcrição Forkhead/imunologia , Transdução de Sinais/genética , Análise de Célula Única/métodos , Linfócitos T Reguladores/imunologia , Transcriptoma/genética , Western Blotting , Ensaio de Imunoadsorção Enzimática , Fatores de Transcrição Forkhead/metabolismo , Doença Enxerto-Hospedeiro/etiologia , Doença Enxerto-Hospedeiro/imunologia , Doença Enxerto-Hospedeiro/metabolismo , Transplante de Células-Tronco Hematopoéticas/métodos , Humanos , RNA-Seq/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Subpopulações de Linfócitos T/imunologia , Subpopulações de Linfócitos T/metabolismo , Linfócitos T Reguladores/metabolismoRESUMO
Gold nanoclusters are promising optically functional materials because of their attractive optical properties, such as luminescence, two-photon absorption, photothermal conversion, and photodynamics. Regulating the optical functions of gold nanoclusters and improving their performance have attracted wide interest in biological applications. In this Review, we introduce the principles to manipulate both the intrinsic optical properties and the apparent optical performance of gold nanoclusters. Manipulating the surface ligands and compounding with other nanomaterials are facile and efficient strategies. Based on the regulated optical properties, the gold nanoclusters can be well applied in various biomedical applications from multimodal bioimaging toward theranostics. By correlating structures and optical properties, we expect a better utilization of the optical gold nanoclusters in the biological field.
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Biomolecule-protected gold nanostructures show good performance in biomedical applications. However, precise control over gold nanocluster (AuNC) preparation with biomolecules remains challenging. Here, we develop a simple near-infrared (NIR)-light-assisted method for in situ reduction of antimicrobial peptide (AMP)-protected AuNCs. Take advantage of the high photothermal conversion efficiency of the conjugated polymer (CP) upon NIR light irradiation, we promote the rapid reduction of AuNCs by the AMP on the surface of the CP. The fluorescent properties of the AuNCs were improved owing to the formation of a unique Au(0)NC@Au(I)AMP core-shell nanostructure. This nanostructure is attributed to the rapid reduction of Au(0) and collision and fusion of Au(0) at high temperatures. Integrating antibacterial AMPs, fluorescent AuNCs, and photothermal CPs, the composites facilitated different killing mechanisms for both bacteria and cancer cells. This material system provides an all-in-one strategy for the stepwise killing of cancer cells and bacterial infection.
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Antibacterianos/química , Antibacterianos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Ouro/química , Peptídeos/química , Peptídeos/farmacologia , Bactérias/efeitos dos fármacos , Linhagem Celular Tumoral , Sobrevivência Celular , Humanos , Raios Infravermelhos , Nanopartículas Metálicas/química , Oxirredução/efeitos da radiaçãoRESUMO
Ligand-protected gold nanoclusters (AuNCs) show promise for high performance in biological applications, such as imaging and therapeutics. The assembly of AuNCs with biological macromolecules represents a simple but effective approach to fine-tuning of material functionalities. Thus, these materials might enable intracellular applications of AuNCs. Herein, we prepared a new AuNC-based nanometric system through a self-assembly approach mediated by hydrophobic and electrostatic effects. We show that hydrophobic and electrostatic effects between fluorescent AuNCs with protamine and hyaluronic acid contribute to the formation of small nanocomposites with acceptable colloidal stability. More importantly, the AuNC-decorated nanocomposites show assembly enhanced emission and singlet oxygen generation. In vitro experiments showed that our nanocomposites labeled specific cells by targeting CD44 and induced cell death by producing singlet oxygen. Hence, our AuNC-decorated nanocomposites show great potential as theranostic fluorescent nanomaterials.
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Ouro , Nanopartículas Metálicas/química , Nanocompostos/química , Oxigênio Singlete , Ouro/química , Ouro/farmacologia , Humanos , Células MCF-7 , Oxigênio Singlete/química , Oxigênio Singlete/farmacologiaRESUMO
Here, we develop bifunctional ligand-engineered gold nanoclusters (AuNCs) as signal amplifying reporters for multimode imaging. Modified streptavidin (SA) and biotin alkyl acid-based ligands were applied to AuNCs to form AuNC-SA and AuNC-biotin. The zwitterionic ligands promoted bioassembly and avoided nonspecific adsorption. The AuNCs resisted aggregation-induced quenching and showed strong emission benefited from biological self-assembly. The engineered AuNCs featured stable emission, a large two-photon absorption cross section, long fluorescence lifetime, and good biocompatibility. Thus, cell-expressed antigen-induced protein-binding events were effectively converted into signals from the biological assemble of AuNCs. We performed a comprehensive assay of specific antigens and the cell structure, through one-photon imaging, two-photon imaging, and fluorescence lifetime imaging of AuNCs in a simple, sensitive, and reliable way.
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Ouro/química , Nanopartículas Metálicas/química , Reações Antígeno-Anticorpo , Biotina/química , Moléculas de Adesão Celular/química , Moléculas de Adesão Celular/imunologia , Humanos , Concentração de Íons de Hidrogênio , Proteínas Imobilizadas/química , Proteínas Imobilizadas/imunologia , Ligantes , Células MCF-7 , Microscopia de Fluorescência , Espectrometria de Fluorescência , Estreptavidina/químicaRESUMO
In this work, we report a flexible film based on conjugated polyelectrolyte/silver nanocomposites with efficient antibacterial activity. A flexible poly(dimethylsiloxane) film served as a substrate for deposition of nanostructured silver. A light-activated antibacterial agent, based on the cationic conjugated polyelectrolyte poly({9,9-bis[6'-(N,N-trimethylamino)hexyl]-2,7-fluorenyleneethynylene}-alt-co-1,4-(2,5-dimethoxy)phenylene)dibromide (PFEMO) was self-assembled on the negatively charged substrate. By changing the thickness of the poly(l-lysine)/poly(acrylic acid) multilayers between the metal substrate and PFEMO, we obtained concomitant enhancement of PFEMO fluorescence, phosphorescence, and reactive oxygen species generation. These enhancements were induced by surface plasmon resonance effects of the Ag nanoparticles, which overlapped the PFEMO absorption band. Owing to the combination of enhanced bactericidal effects and good flexibility, these films have great potential for use as novel biomaterials for preventing bacterial infections.
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Fluorescent gold nanoclusters (AuNCs) have drawn considerable research interest owing to their unique emission properties. However, the environment surrounding the NC greatly influences its luminous behavior. In this work, a novel nanocomposite based on AuNCs with bright fluorescence and high biocompatibility was prepared. In this nanocomposite, mesoporous silica nanospheres provided a mesoporous framework, which helped to template the formation of ultrasmall AuNCs and also prevented their aggregation in different solutions. These nanocomposites emitted stable fluorescence even in complex biological environments. After the self-assembly of folic acid-conjugated poly(l-lysine), the presence of folic acid on the nanocomposites guaranteed a good recognition in folate receptor (FR)-positive cells, improving detection selectivity. Cellular experiments demonstrated that the nanocomposites had good dispersity in the physiological environment and could be internalized by FR-positive cancer cells, resulting in bright fluorescence. We believe that this research provides a simple approach to the fabrication of stable fluorescent AuNC nanocomposites, which show good compatibility with complex biological systems and great potential for applications in biological imaging and cell detection.
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Nanocompostos , Ouro , Nanopartículas Metálicas , Dióxido de Silício , Espectrometria de FluorescênciaRESUMO
Regularity of greenhouse gas emissions from black soil with different reclamation years in northern China was investigated by an incubation experiment. Soil samples cultivated for 2 years, 30 years, 100 years and uncultivated were collected and incubated at 2 degrees C and a soil moisture of 60% of the water hold capacity (WHC) for 7 days. The results indicated that the physical and chemical properties of black soil changed significantly after reclamation, which had significant influence on the greenhouse gas emissions. N2O emission was stimulated after soil was reclaimed, the longer time of reclamation, the higher N2O emitted from soil, and the N2O emissions from soil cultivated for 30 and 100 years were significantly higher than that from uncultivated soil. There were significant positive correlations between N2O emission and the content of water organic nitrogen and silt in soil, whereas significant negative correlations were found between pH, sand content and N2O emission. CO2 emission decreased after the soil was cultivated, and CO2 emission from soil cultivated for 30 and 100 years were significantly lower than that of uncultivated soil. There were significant positive correlations between organic carbon, water organic carbon and CO2 emission. All of the soils behaved as weak sources of CH4 emission during the first 4 days of incubation, and then behaved as weak sink of atmospheric CH4, the CH4 cumulative emission increased with reclamation years. The difference of CO2 and CH4 emissions from black soil with different reclamation years may be attributed to the difference of soil pH, organic carbon, soluble organic carbon and the contents of sand and silt.