Associations of MCM8 rs3761873 and rs16991617 variants with abnormal uterine bleeding induced by copper intrauterine device.

AIM: Intrauterine device (IUD) is a commonly used contraceptive method worldwide. Abnormal uterine bleeding (AUB) is one of the most common side effects of Cu-IUDs. Since AUB varies among Cu-IUD users, changes in the bleeding-related genetic factors may contribute to AUB. This study aimed to determine the genetic risk factors of AUB after Cu-IUD insertion. METHODS: We conducted a case-control study on women who experienced AUB after Cu-IUD insertion (case:control = 62:59). Six candidate variants were genotyped using the Sequenom MassARRAY. Genotype and allele frequencies were analyzed using SHEsisPlus. We performed Pearson's Chi-squared test to analyze categorical data, and ESEfinder to predict the impact on splicing regulation. RESULTS: MCM8 coding sequence variants: rs3761873-A>C was in Exon 7 and rs16991617 A>G was in Exon 12 of all 19 exons, both of which were significantly different between cases and controls (pallele  = 0.039 and pgenotype  = 0.092). rs6022 and rs6029 in F5 gene and rs3761873 and rs16991617 in the MCM8 gene showed strong linkage disequilibrium (R2 > 0.8). ESEfinder indicated that the variants of MCM8 may affect the splicing regulation. CONCLUSIONS: MCM8 rs376187 and rs16991617 were associated with AUB in Cu-IUDs users. MCM8 may play a role in AUB by regulating functions of reproductive organs and primary ovarian insufficiency. Our findings may improve the understanding of the genetic basis of AUB caused by Cu-IUDs.

Epigenetic alternations of microRNAs and DNA methylation contribute to gestational diabetes mellitus.

This study aimed to identify epigenetic alternations of microRNAs and DNA methylation for gestational diabetes mellitus (GDM) diagnosis and treatment using in silico approach. Data of mRNA and miRNA expression microarray (GSE103552 and GSE104297) and DNA methylation data set (GSE106099) were obtained from the GEO database. Differentially expressed genes (DEGs), differentially expressed miRNAs (DEMs) and differentially methylated genes (DMGs) were obtained by limma package. Functional and enrichment analyses were performed with the DAVID database. The protein-protein interaction (PPI) network was constructed by STRING and visualized in Cytoscape. Simultaneously, a connectivity map (CMap) analysis was performed to screen potential therapeutic agents for GDM. In GDM, 184 low miRNA-targeting up-regulated genes and 234 high miRNA-targeting down-regulated genes as well as 364 hypomethylation-high-expressed genes and 541 hypermethylation-low-expressed genes were obtained. They were mainly enriched in terms of axon guidance, purine metabolism, focal adhesion and proteasome, respectively. In addition, 115 genes (67 up-regulated and 48 down-regulated) were regulated by both aberrant alternations of miRNAs and DNA methylation. Ten chemicals were identified as putative therapeutic agents for GDM and four hub genes (IGF1R, ATG7, DICER1 and RANBP2) were found in PPI and may be associated with GDM. Overall, this study identified a series of differentially expressed genes that are associated with epigenetic alternations of miRNA and DNA methylation in GDM. Ten chemicals and four hub genes may be further explored as potential drugs and targets for GDM diagnosis and treatment, respectively.

Return-Sludge Treatment with Endogenous Free Nitrous Acid Limits Nitrate Production and N2O Emission for Mainstream Partial Nitritation/Anammox.

Nitrite oxidizing bacteria (NOB) and nitrous oxide (N2O) hinder the development of mainstream partial nitritation/anammox. To overcome these, endogenous free ammonia (FA) and free nitrous acid (FNA), which can be produced in the sidestream, were used for return-sludge treatment for two integrated-film activated sludge reactors containing biomass in flocs and on carriers. The repeated exposure of biomass from one reactor to FA shocks had a limited impact on NOB suppression but inhibited anammox bacteria (AnAOB). In the other reactor, repeated FNA shocks to the separated flocs failed to limit the system's nitrate production since NOB activity was still high on the biofilms attached to the unexposed carriers. In contrast, the repeated FNA treatment of flocs and carriers favored aerobic ammonium-oxidizing bacteria (AerAOB) over NOB activity with AnAOB negligibly affected. It was further revealed that return-sludge treatment with higher FNA levels led to lower N2O emissions under similar effluent nitrite concentrations. On this basis, weekly 4 h FNA shocks of 2.0 mg of HNO2-N/L were identified as an optimal and realistic treatment, which not only enabled nitrogen removal efficiencies of ∼65% at nitrogen removal rates of ∼130 mg of N/L/d (20 °C) but also yielded the lowest cost and carbon footprint.

Association of UHRF1 gene polymorphisms with oligospermia in Chinese males.

BACKGROUND: UHRF1 plays an important role in maintaining DNA methylation patterns during spermatogenesis. This study was performed to evaluate the association between UHRF1 gene variations and infertility in males with oligozoospermia in a Chinese population. METHODS: In this case-control study of 735 Chinese men, single-nucleotide polymorphism (SNP) genotypes and alleles in the UHRF1 gene were assessed by direct sequencing. The effects of the mutations on UHRF1 transcription were investigated using a dual-luciferase reporter gene assay. RESULTS: We identified 24 SNPs, including nine SNPs in the promoter region, three in the 5' untranslated region, five in introns, and seven in exons. Interestingly, the genotype frequencies of SNP rs2656927 (P = 0.014) and rs8103849 (P < 0.001) significantly differed between men with oligozoospermia in case group 1 and normozoospermic men. Moreover, four variants (three were novel) were detected only in the patient group, with two in introns and the others in the promoter region. The results of the luciferase assay showed that the -1615C>T-C and -1562A>G-A alleles increased luciferase activity compared with the -1615C>T-T and -1562A>G-G alleles. CONCLUSIONS: We detected two SNPs in the UHRF1 gene showing a significant difference between the case and control groups. Two screened SNPs affected UHRF1 promoter activity, improving the understanding of the pathophysiology of oligozoospermia.

Nitrogen removal performance of anaerobic ammonia oxidation (ANAMMOX) in presence of organic matter.

A sequencing batch reactor (SBR) was used to test the nitrogen removal performance of anaerobic ammonium oxidation (ANAMMOX) in presence of organic matter. Mesophilic operation (30 ± 0.5 °C) was performed with influent pH 7.5. The results showed, independent of organic matter species, ANAMMOX reaction was promoted when COD was lower than 80 mg/L. However, specific ANAMMOX activity decreased with increasing organic matter content. Ammonium removal efficiency decreased to 80% when COD of sodium succinate, sodium potassium tartrate, peptone and lactose were 192.5, 210, 225 and 325 mg/L, respectively. The stoichiometry ratio resulting from different OM differed largely and R1 could be as an indicator for OM inhibition. When COD concentration was 240 mg/L, the loss of SAA resulting from lactose, peptone, sodium potassium tartrate and sodium succinate were 28, 36, 50 and 55%, respectively. Sodium succinate had the highest inhibitory effect on SAA. When ANAMMOX process was used to treat wastewater containing OM, the modified Logistic model could be employed to predict the NREmax.

Effect of influent substrate ratio on anammox granular sludge: performance and kinetics.

Effect of influent substrate ratio on anammox process was studied in sequencing batch reactor. Operating temperature was fixed at 35 ± 1 °C. Influent pH and hydraulic retention time were 7.5 and 6 h, respectively. When influent NO2--N/NH4+-N was no more than 2.0, total nitrogen removal rate (TNRR) increased whereas NH4+-N removal rate stabilized at 0.32 kg/(m3 d). ΔNO2--N/ΔNH4+-N increased with enhancing NO2--N/NH4+-N. When NO2--N/NH4+-N was 4.5, ΔNO2--N/ΔNH4+-N was 1.98, which was much higher than theoretical value (1.32). The IC50 of NO2--N was 289 mg/L and anammox activity was inhibited at high NO2--N/NH4+-N ratio. With regard to influent NH4+-N/NO2--N, the maximum NH4+-N removal rate was 0.36 kg/(m3 d), which occurred at the ratio of 4.0. Anammox activity was inhibited when influent NH4+-N/NO2--N was higher than 5.0. With influent NO3--N/NH4+-N of 2.5-6.5, NH4+-N removal rate and NRR were stabilized at 0.33 and 0.40 kg/(m3 d), respectively. When the ratio was higher than 6.5, nitrogen removal would be worsened. The inhibitory threshold concentration of NO2--N was lower than NH4+-N and NO3--N. Anammox bacteria were more sensitive to NO2--N than NH4+-N and NO3--N. TNRR would be enhanced with increasing nitrogen loading rate, but sludge floatation occurred at high nitrogen loading shock. The Han-Levenspiel could be applied to simulate nitrogen removal resulting from NO2--N inhibition.

Nitrogen removal performance and operation strategy of anammox process under temperature shock.

Sequencing batch reactors were used to study anaerobic ammonium oxidation (anammox) process under temperature shock. Both long-term (15-35 °C) and short-term (10-50 °C) temperature effects on nitrogen removal performance were performed. In reactor operation test, the results indicated that ammonium removal rate decreased from 0.35 kg/(m3 day) gradually to 0.059 kg/(m3 day) when temperature dropped from 35 to 15 °C. Although bacteria morphology was not modified, sludge settling velocity decreased with decreasing temperature. In batch test, apparent activation energy (Ea) increased with decreasing temperature, which suggested the activity decrease of anaerobic ammonium oxidizing bacteria (AAOB). Low temperature inhibited AAOB and weakened nitrogen removal performance. The cardinal temperature model with inflection was first used to describe temperature effect on anammox process. Simulated results revealed that anammox reaction could occur at 10.52-50.15 °C with maximum specific anammox activity of 0.50 kg/(kg day) at 36.72 °C. The cold acclimatization of AAOB could be achieved and glycine betaine could slightly improve nitrogen removal performance at low temperature.

Performance and kinetics of ANAMMOX granular sludge with pH shock in a sequencing batch reactor.

As an efficient and cost-effective nitrogen removal process, anaerobic ammonium oxidation (ANAMMOX) could be well operated at suitable pH condition. However, pH shock occurred in different kinds of wastewater and affected ANANNOX process greatly. The present research aimed at studying the performance and kinetics of ANAMMOX granular sludge with pH shock. When influent pH was below 7.5, effluent [Formula: see text]-N and [Formula: see text]-N increased with decreasing pH. At Ph 6.0, effluent [Formula: see text]-N approached 100 mg/L, and the ratios of [Formula: see text] approached 2.2 and 1.3, respectively. Both greatly deviated from theoretical values. When influent pH was above 7.5, effluent [Formula: see text]-N and [Formula: see text]-N increased with increasing pH. At pH 9.0, ammonium removal rate (ARR) and nitrite removal rate (NRR) decreased to 0.011 ± 0.004 and 0.035 ± 0.004 kg/(m3·d), respectively. Besides, [Formula: see text]-N:[Formula: see text]-N deviated from theoretical value. Longer recovery time from pH 9.0 than from pH 6.0 indicated that alkaline surroundings inhibited anaerobic ammonium oxidizing bacteria (AAOB) greater. The sludge settling velocity was 2.15 cm/s at pH 7.5. However, it decreased to 2.02 cm/s when pH was 9.0. Acidic pH had little effect on sludge size, but disintegration of ANAMMOX granule was achieved with pH of 9.0. The Bell-shaped (A) model and the Ratkowsky model were more applicable to simulate the effect resulting from pH shock on ANAMMOX activity (R2 > 0.95), and both could describe ANAMMOX activity well with pH shock. They indicated that qmax was 0.37 kg [Formula: see text]-N/(kgMLSS·d) at the optimum pH value (7.47) in present study. The minimum pH during which ANAMMOX occurred was 5.68 while the maximum pH for ANAMMOX reaction was 9.26. Based on nitrogen removal performance with different pH, strongly acidic (pH ≤ 6.5) or alkaline (pH ≥ 8.5) inhibited ANAMMOX process. Besides, ANAMMOX appeared to be more susceptible to alkaline wastewater. Compared to extremely acidic condition (low pH), extremely alkaline condition (high pH) affected ANAMMOX granules much more.

An innovative exploration to identify and isolate the dominant-flocculated-species from polytitanium chloride synthesized by electrodialysis.

Abundance of dominant-flocculated-species is the key to determine coagulation performance of coagulant. Titanium-based coagulants have garnered considerable attention due to their high coagulation efficiency, but with a current challenge of the identification and isolation of the dominant-flocculated-species. Herein, polytitanium chloride (PTC), enriched with dominant-flocculated-species, was successfully synthesized by electrodialysis through accurate micro-interface control of the reaction among Ti-hydrolyzed-species and OH-. Special attention was paid to a feasible and high-effective strategy to isolate the dominant-flocculated-species from PTC through one-step rapid ultrafiltration. Selective preference was the ultrafiltration membranes (made of polyethersulfone) with a molecular weight cut-off of 5 kDa, which enabled the isolation of the dominant-flocculated-species, named PTC-5k. Results from the electrospray time-of-flight mass spectrometry (ESI-TOF-MS) proved a large proportion of the small and medium-sized hydrolyzed products as dominant-flocculated-species in PTC-5k, with the main signals concentrated between m/z 100 and 500. This composition achieved approximately 15.0% higher removal of organic matter with a 33.0% reduction in dosage compared to PTC. Unique snowflake-like branched structure of PTC-5k enhanced the coagulation mechanisms of sweeping and adsorption-bridging flocculation. Worth noting was the more compact flocs formed by PTC-5k than PTC, which was the probable reason for the mitigated fouling of ceramic membrane when PTC-5k was utilized as pre-treatment methodology. Continuous operation of ceramic membrane filtration up to 30 h, demonstrated 30% improvement in stable flux compared to PTC. This study provides the strategy for the isolation of Ti-dominant-flocculated-species, and lays the foundation for practical application.

Corrigendum: Dilemma and countermeasure of sustainable leadership in physical education development in southern rural Ningxia, China.

[This corrects the article DOI: 10.3389/fpsyg.2022.947694.].

Peer effects and health impacts of different body cognitive biases in children: micro evidence from China.

Background: In China, children commonly display body cognitive biases, which constitute a significant yet hidden public health issue. These biases potentially jeopardize children's well-being, hinder the cultivation of human capital, and impede societal progress. However, limited research employs theoretical analysis and econometric testing to investigate the formation of different body cognitive biases among Chinese children and their health impacts. Methods: Based on a local average network model for theoretical analysis, this study utilizes a sample of 4,289 children from four phases of the China Health and Nutrition Survey (CHNS) conducted from 2004 to 2011. Utilizing Logit and IV Probit models, systematically evaluate the peer effect, heterogeneity of effects, and health impacts of children's different body cognitive biases. Results: (1) The peer effect contributes to the development of light- and heavy-body cognitive biases in Chinese children. (2) The heterogeneity analysis shows that the peer effect of body cognitive biases is more significant in rural and female children. (3) The influence of heavy-body cognitive bias is more pronounced in adolescent children. (4) The "eating-activity balance" is disrupted by the two body cognitive biases in children, leading to deviations from normal body type. (5) Specifically, the light-body cognitive bias leads children to intake more and burn fewer calories, increasing their risk of obesity. (6) Conversely, the heavy-body cognitive bias prompts children to intake less and expend more calories, resulting in a higher prevalence of thinness. Discussion: This study innovates by exploring peer effects on body cognitive biases in Chinese children, elucidating their direction and health implications. While overweight and obesity are recognized as overt health issues, the spread and impact of implicit issues like body cognitive biases should not be overlooked. Nevertheless, the issue is largely neglected in developing countries, such as China, where existing children's health policies are inadequate in addressing it. Promoting accurate body image perception and understanding of health prevention strategies among children requires adequate attention to peer effects.

Earthquake focal mechanisms with distributed acoustic sensing.

Earthquake focal mechanisms provide critical in-situ insights about the subsurface faulting geometry and stress state. For frequent small earthquakes (magnitude< 3.5), their focal mechanisms are routinely determined using first-arrival polarities picked on the vertical component of seismometers. Nevertheless, their quality is usually limited by the azimuthal coverage of the local seismic network. The emerging distributed acoustic sensing (DAS) technology, which can convert pre-existing telecommunication cables into arrays of strain/strain-rate meters, can potentially fill the azimuthal gap and enhance constraints on the nodal plane orientation through its long sensing range and dense spatial sampling. However, determining first-arrival polarities on DAS is challenging due to its single-component sensing and low signal-to-noise ratio for direct body waves. Here, we present a data-driven method that measures P-wave polarities on a DAS array based on cross-correlations between earthquake pairs. We validate the inferred polarities using the regional network catalog on two DAS arrays, deployed in California and each comprising ~ 5000 channels. We demonstrate that a joint focal mechanism inversion combining conventional and DAS polarity picks improves the accuracy and reduces the uncertainty in the focal plane orientation. Our results highlight the significant potential of integrating DAS with conventional networks for investigating high-resolution earthquake source mechanisms.

Development and validation of the Chinese family environment influencing physical activity habits scale.

Introduction: Adolescence represents a pivotal stage in the development of healthy behaviors, where establishing positive physical activity habits can have enduring effects on an individual's overall wellbeing. The ecological model highlights the influence of environmental factors on human behavior, with the family environment playing a significant role in shaping the physical activity habits of adolescents. Methods: The aim of this scale is to develop a reliable and effective tool, customized for the unique societal context of China, to assess how family factors influence the physical activity habits of Chinese adolescents. Participants were recruited through stratified cluster sampling from 24 secondary schools in six Chinese provinces between October and November 2021, resulting in 1,061 participants. Analysis was conducted on 1,004 valid questionnaires, divided into two samples. Sample 1, consisting of 502 students (248 males and 254 females; Mage = 15.5), underwent item analysis and exploratory factor analysis. Sample 2, comprising 502 students (267 males and 235 females; Mage = 16.5), underwent confirmatory factor analysis and internal consistency reliability analysis. Results: Through exploratory factor analysis, we extracted three factors comprising 15 items: "Family Environment Construction" (4 items), "Family Action Support" (6 items), and "Family Health Awareness" (5 items). The Cronbach's alpha values for these factors ranged from 0.890 to 0.894. Confirmatory factor analysis confirmed a satisfactory model fit (CMIN/DF = 1.45, SRMR = 0.027, GFI = 0.991, TLI = 0.989, RMSEA = 0.03). Discussion: The scale demonstrated strong internal consistency and test-retest reliability, confirming its effectiveness in empirical research. This study holds significant implications for enhancing the physical activity levels of adolescents, promoting their physical and mental wellbeing, enriching their developmental experiences, and contributing to their overall sense of happiness.

Comparison of typical nitrite oxidizing bacteria suppression strategies and the effect on nitrous oxide emissions in a biofilm reactor.

In mainstream partial nitritation/anammox (PN/A), suppression of nitrite oxidizing bacteria (NOB) and mitigation of N2O emissions are two essential operational goals. The N2O emissions linked to three typical NOB suppression strategies were tested in a covered rotating biological contactor (RBC) biofilm system at 21 °C: (i) low dissolved oxygen (DO) concentrations, and treatments with (ii) free ammonia (FA), and (iii) free nitrous acids (FNA). Low emerged DO levels effectively minimized NOB activity and decreased N2O emissions, but NOB adaptation appeared after 200 days of operation. Further NOB suppression was successfully achieved by periodic (3 h per week) treatments with FA (29.3 ± 2.6 mg NH3-N L-1) or FNA (3.1 ± 0.3 mg HNO2-N L-1). FA treatment, however, promoted N2O emissions, while FNA did not affect these. Hence, biofilm PN/A should be operated at relatively low DO levels with periodic FNA treatment to maximize nitrogen removal efficiency while avoiding high greenhouse gas emissions.

The magmatic web beneath Hawai'i.

The deep magmatic architecture of the Hawaiian volcanic system is central to understanding the transport of magma from the upper mantle to the individual volcanoes. We leverage advances in earthquake monitoring with deep learning algorithms to image the structures underlying a major mantle earthquake swarm of nearly 200,000 events that rapidly accelerated after the 2018 Kilauea caldera collapse. At depths of 36 to 43 kilometers, we resolve a 15-kilometers-long collection of near-horizontal sheeted structures that we identify as a sill complex. These sills connect to the lower depths of Kilauea's plumbing by a 25-kilometers-long belt of seismicity. Additionally, a column of seismicity links the sill complex to a shallow décollement near Mauna Loa. These findings implicate the mantle sill complex as a nexus for magma transport beneath Hawai'i and furthermore indicate widespread magmatic connectivity in the volcanic system.

Feasibility of a return-sludge nursery concept for mainstream anammox biostimulation: Creating optimal conditions for anammox to recover and grow in a parallel tank.

To overcome limiting anammox activity under sewage treatment conditions, a return-sludge nursery concept is proposed. This concept involves blending sludge reject water treated with partial nitritation with mainstream effluent to increase the temperature, N levels, and electrical conductivity (EC) of the anammox nursery reactor, which sludge periodically passes through the return sludge line of the mainstream system. Various nursery frequencies were tested in two 2.5 L reactors, including 0.5-2 days of nursery treatment per 3.5-14 days of the total operation. Bioreactor experiments showed that nursery increased nitrogen removal rates during mainstream operation by 33-38%. The increased anammox activity can be partly (35-60%) explained by higher temperatures. Elevated EC, higher nitrogen concentrations, and a putative synergy and/or unknown factor were responsible for 15-16%, 12-14%, and 10-36%, respectively. A relatively stable microbial community was observed, dominated by a "Candidatus Brocadia" member. This new concept boosted activity and sludge growth, which may facilitate mainstream anammox implementations based on partial nitritation/anammox or partial nitrification/denitratation/anammox.

An upper-crust lid over the Long Valley magma chamber.

Geophysical characterization of calderas is fundamental in assessing their potential for future catastrophic volcanic eruptions. The mechanism behind the unrest of Long Valley Caldera in California remains highly debated, with recent periods of uplift and seismicity driven either by the release of aqueous fluids from the magma chamber or by the intrusion of magma into the upper crust. We use distributed acoustic sensing data recorded along a 100-kilometer fiber-optic cable traversing the caldera to image its subsurface structure. Our images highlight a definite separation between the shallow hydrothermal system and the large magma chamber located at ~12-kilometer depth. The combination of the geological evidence with our results shows how fluids exsolved through second boiling provide the source of the observed uplift and seismicity.

Seismic arrival-time picking on distributed acoustic sensing data using semi-supervised learning.

Distributed Acoustic Sensing (DAS) is an emerging technology for earthquake monitoring and subsurface imaging. However, its distinct characteristics, such as unknown ground coupling and high noise level, pose challenges to signal processing. Existing machine learning models optimized for conventional seismic data struggle with DAS data due to its ultra-dense spatial sampling and limited manual labels. We introduce a semi-supervised learning approach to address the phase-picking task of DAS data. We use the pre-trained PhaseNet model to generate noisy labels of P/S arrivals in DAS data and apply the Gaussian mixture model phase association (GaMMA) method to refine these noisy labels and build training datasets. We develop PhaseNet-DAS, a deep learning model designed to process 2D spatio-temporal DAS data to achieve accurate phase picking and efficient earthquake detection. Our study demonstrates a method to develop deep learning models for DAS data, unlocking the potential of integrating DAS in enhancing earthquake monitoring.

Predictive plasma biomarker for gestational diabetes: A case-control study in China.

OBJECTIVE: This study aims to find new plasma biomarkers in early pregnancy. DESIGN: The original study enrolled 1219 pregnant women. We investigated protein expression profiles of placental tissues from women with GDM (n = 89) and normal glucose tolerance (NGT) (n = 83). Maternal plasma samples between two groups in early and middle pregnancy were used for validation of candidate biomarkers. METHODS: Differentially expressed proteins (DEPs) were identified by label-free quantitative proteomics from human placenta samples between two groups. Several DEPs were validated in plasma by Luminex assays. An automatic biochemical analyzer was used to detect blood lipid indexes. The associations of GAL-3BP with biochemical indicators were demonstrated by Pearson's correlation analysis. Binary logistic regression was used to model potential predictive indicators in early pregnancy of GDM. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic accuracy of the predictive model and the value of GAL-3BP. RESULTS: 123 DEPs were found in placenta involved in ribosomal function, pancreatic secretion, oxidative phosphorylation, and inflammatory signaling pathway. Plasma GAL-3BP are significantly higher in women with GDM than NGT in the first (p = 0.008) and second (p = 0.026) trimester, but C9 and VWF have no difference. The predictive value of GAL-3BP in the first trimester of pregnancy (AUC 0.64) is better than that in the second trimester (AUC 0.61), and combined predictive model of TG and GAL-3BP at early pregnancy has greater predictive and diagnostic value for GDM (AUC 0.69) than individual GAL-3BP (AUC 0.64). CONCLUSIONS: Plasma TG and GAL-3BP has good predictive and diagnostic value at early pregnancy, suggesting that these two indicators may be used as biomarkers for early prediction and diagnosis of GDM. SIGNIFICANCE: The advantage of this study is that circulating TG and GAL-3BP might differentiate the progress of women with GDM and normal glucose tolerance (NGT) at the early stage of pregnancy. It is the first study to consider the role of GAL-3BP as an early predictive biomarker in the development of GDM during the whole pregnancy. Another advantage is that volunteers in this study were recruited from two provinces in China to eliminate the impacts of environmental confounders. The similar changes of blood glucose/lipid indicators for women with GDM and NGT in both regions was found in the first and second trimester of pregnancy, which added to the reliability of analytical results.

Dilemma and countermeasure of sustainable leadership in physical education development in southern rural Ningxia, China.

Globally, above 1.4 billion adults did not reach the recommended level of physical education in their daily life, thus triple intendent reforms are proposed by the Ministry of Physical Education for the development of sporting leadership in schools, colleges, and universities, which are essentially important for the development of physical and mental health of the students. This article analyzes the situation of lacking sustainable sporting leadership among other factors related to Physical Education (PE) resources in the southern areas of Ningxia. A mixed and multi-method approach was adopted to conduct the study. First, an in-depth but an open-ended qualitative interview with the professionals was carried out, followed by cross-sectional data collected from the respondents in two districts of the southern mountainous area of Ningxia. Moreover, a case study was included to support the phenomenon from a contextual perspective. The study present that PE education needs a modernization and rejuvenation plan to link with PE development and its sustainable execution and implementation for the physical and mental development of the learners. Moreover, it is suggested to strengthen the development of physical education with/through the fields of regional integration of educational resources sharing, cultural elements and integration, latest technological tools, research-based and cultural supported curriculum, and endogenous strength construction to promote the development of school physical education. Furthermore, continuous monitoring and evaluation mechanisms need to be adopted to develop physical education in the region.