First detection of Chlamydia trachomatis LGV biovar in the Czech Republic, 2010-2011.

We present four cases of proctitis in HIV-infected men having sex with men (MSM) living in the Czech Republic. The causative agent in all cases was the lymphogranuloma venereum (LGV) biovar of Chlamydia trachomatis. The spread of proctitis caused by C. trachomatis serovars L1­3 among MSM has been observed in several European countries, the United States and Canada since 2003. To our knowledge, no LGV cases in eastern Europe have been published to date.

Genetic variability of cytomegalovirus glycoprotein O in hematopoietic stem cell transplant recipients.

UNLABELLED: Genetic variation of cytomegalovirus (CMV) strains can correlate with their pathogenicity for immunocompromised patients. Glycoprotein O (gO), together with glycoprotein L and glycoprotein H, mediate the fusion of the viral envelope with the cell membrane and promotes virus penetration, envelopment, and release. The variability of gO might play a role in CMV cell tropism. The goal was a retrospective analysis of gO variability in a cohort of hematopoietic stem cell transplant (HSCT) recipients to determine the distribution of gO genotypes and to investigate their impact on clinical outcome and manifestation of CMV infection. METHODS: In archived blood samples from 51 adult allogeneic HSCT recipients with active CMV infection, gO was analyzed by sequencing the N-terminal domain of the UL74 gene using the dye deoxy termination method. RESULTS: The gO1 and gO2 clades were most common (39% and 20%, respectively, and gO3 was associated with higher risk of symptomatic infection (P = 0.026 in multivariant analysis). Despite being associated with higher antigenemia levels (P = 0.02), gO4 had the best survival and lower rate of CMV recurrence. No significant differences were found in clinical manifestation and outcome of CMV disease between patients with various gO clades. Because CMV strains sharing an identical gO sequence differed in glycoprotein B genotypes, sequencing the N-terminal part of the gO gene does not seem to be optimal for the identification of strains. CONCLUSIONS: gO genotyping may contribute to the biological characterization of CMV strains in HSCT recipients.

[Genotyping of viral glycoprotein B (gB) in hematopoietic stem cell transplant recipients with active cytomegalovirus infection: analysis of the impact of gB genotypes on the patients' outcome]. / Genotypizace virového glykoproteinu B (gB) u príjemcu transplantátu kmenových bunek krvetvorby s aktivní infekcí cytomegalovirem--sledováni vlivu genotypu na prubeh infekce.

AIM OF THE STUDY: Genetic variation of CMV strains may correlate with their pathogenicity for immunocompromised patients. On the basis of sequence variation in the UL55 gene encoding the most abundant viral envelope glycoprotein gB, CMV can be classified into four major gB genotypes. The aim of the study was the analysis of the distribution of gB genotypes in a cohort of haematopoietic stem cell transplant (HSCT) recipients and of the correlation of genetic polymorphisms with clinical outcomes and manifestation of CMV infection. MATERIAL AND METHODS: Archived DNA isolates from consecutive blood samples of 53 adult allogeneic HSCT recipients with active CMV infection, transplanted in 2004-2005, were used for the genetic analysis. HCMV gB genotyping was performed by restriction fragment length polymorphism (RFLP) analysis and sequencing of the central variable region of UL55. The association of gB genotypes with selected clinical parameters was assessed by multivariate analysis after adjustment for graft donor type, HLA-matching and anti-thymocyte immunoglobulin (ATG) therapy. RESULTS: gB1, gB2, gB3, and gB4 genotypes were detected in 30%, 17%, 26% and 4% of the patients, respectively. An atypical gB genotype was found in one patient. Co-infection with two or more gB genotypes was revealed in 17% of the patients. The distribution of gB genotypes did not vary in time, despite the fact that the patients transplanted in 2005 had more severe CMV infection with higher viral loads in the blood than those transplanted in 2004. gB1 was associated with a lower viral load (p = 0.046) and a milder course of symptomatic CMV infection, but with a higher rate of acute graft versus host disease (OR 3.4; p = 0.067). Pancytopenia was less frequent in the patients infected with gB3 (OR 0.09; p = 0.075). In contrast, gB2-infected patients had a worse outcome of CMV infection with a higher rate of organ involvement and were less responsive to antiviral therapy (OR 6.65 and 0.18; p = 0.15 and 0.12, respectively). The prognostic impact of co-infection with two or more gB genotypes was not shown. CONCLUSIONS: gB genotype may have an impact on the course of CMV infection and its complications in HSCT recipients. Nevertheless, these results need to be tested on a larger group of patients in the context of genetic variability of other functionally important viral genes. The characterization of viral genetic factors determining CMV pathogenesis will be of relevance to the treatment of patients at high risk of CMV infection.

[Prevalence of glycoprotein B (gB) genotypes in the patients with high risk of symptomatic cytomegalovirus infection]. / Zastoupení ruzných genotypu glykoproteinu gB u pacientu s vysokým rizikem symptomatické infekce lidským cytomegalovirem (CMV).

OBJECTIVES: Based on genetic variability of the dominant envelope glycoprotein (gB), human cytomegalovirus is classified into four major genotypes. The aims were to determine the prevalence of particular gB genotypes in Czech CMV-infected patients and to compare three groups of the patients with high risk of symptomatic CMV infection, i.e., haematopoietic stem cell transplant (HSCT) recipients, HIV-positive persons and infants. MATERIAL AND METHODS: The study was performed on 134 archived CMV-positive DNA isolates from the patients tested in the National Reference Laboratory for Herpesviruses in 2004-2007. For genotyping, the variable part of gB was amplified and analysed using the restriction fragment length polymorphism (RFLP) method. RESULTS: The most frequently detected genotype was gB1 (33%), followed by gB2 (29%), gB3 (18%) and gB4 (7%). However, the distribution of gB genotypes varied between groups of high-risk patients: gB2 dominated in HIV-positives (55%, p = 0.004), while gB3 was most common in HSCT recipients (26%, p = 0.03) and gB4 was relatively more frequent in infants (20%, p = 0.03). In HSCT recipients, we found increased frequency of gB3 (26%, p = 0.03) and co-infection with two or more gB genotypes (17%, p = 0.016). CONCLUSIONS: The distribution of CMV gB genotypes from Czech CMV-infected patients is similar to that reported in other European countries or in the United States. Differences in the prevalence of CMV gB genotypes between groups of high-risk patients indicate variation in biological properties of particular gB genotypes, possibly resulting in distinct virulence, immunogenicity or epidemiological characteristics of circulating strains.