Electrophoretic behaviour of yeast mitochondrial translation products.

We have studied the mobility of yeast mitochondrial translation products during electrophoresis on polyacrylamide gels of different composition and found that these polypeptides can be divided into two groups. One, to which subunit II of cytochrome c oxidase belongs, behaves normal as all water-soluble reference proteins. The other, to which cytochrome b and subunits I and III of cytochrome c oxidase belong, shows a free electrophoretic mobility about twice as fast as the first group. Conditions have been found to separate cytochrome c1 from cytochrome b.

Modification of telomeric DNA in Trypanosoma brucei; a role in antigenic variation?

Expression of surface antigen genes in Trypanosoma brucei occurs at expression sites located near telomeres. Since only one antigen is produced at a time, a mechanism must exist to prevent the simultaneous activity of multiple expression sites. Here we report that PstI and PvuII restriction sites in silent telomeric antigen genes are partially uncleavable , presumably as a consequence of DNA modification. The modification, which is absent in transcribed genes but returns after gene inactivation, may be specific for telomeric DNA because (1) it is not detected in non-telomeric genes; (2) modification is highest close to the telomere; (3) the level of modification in a telomeric gene is influenced by the size of the telomeric DNA segment downstream. Whether telomere modification is cause or consequence of antigen gene switch-off remains to be determined.

Physical mapping of 4S RNA genes on chloroplast DNA of Spirodela oligorhiza.

We have determined the position of Spirodela oligorhiza chloroplast 4S RNA genes on the restriction fragment map of cp DNA, using purified in vitro[(32)P]-labeled 4S RNA. The overall organization of these genes is very similar to the organization of tRNAs on spinach cp DNA (Driesel et al. 1979).

The organization of the genes for ribosomal RNA on mitochondrial DNA of Kluyveromyces lactis.

We have constructed a physical map of Kluyveromyces lactis mtDNA using the restriction enzymes HindII and HindIII. In contrast to Saccharomyces, the genes for the large and small ribosomal RNAs are much closer to each other, being separated by a maximal distance of 2,250 base pairs.

Var1 is associated with the small ribosomal subunit of mitochondrial ribosomes in yeast.

We show that the mitochondrial protein known as Var1 is identical with the ribosome-associated protein previously identified as a product of mitochondrial protein synthesis. Although indirect evidence suggests that Var1 is not involved in ribosome biosynthesis, a definite conclusion has to await further investigation.

RNA synthesis in isolated yeast mitochondria.

Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide sequences transcribed from this intervening sequence. In omega- strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed. These results suggest that isolated yeast mitochondria are capable of carrying out RNA synthesis and processing, including splicing.