RESUMO
Preimmunization of BN recipients with 1 ml of WAG/Rij blood 7 days before grafting led to accelerated rejection of WAG/Rij hearts in 5 days (normal, 8 to 9 days) and of (WAG/Rij X BN)F1 hearts in 7 to 11 days (normal, 9 to 11 days). Preimmunization with mixtures of 1 ml of WAG/Rij blood and 0.25 ml of BN anti-WAG/Rij serum (antidonor serum) prevented the accelerated rejection in the WAG/Rij to BN combination and even led to prolonged graft survival. However, using the (WAG/Rij X BN)F1 as donor, this pretreatment led to accelerated rejection as well as prolonged graft survival. Preimmunization of BN recipients with 1 ml of WAG/Rij blood and antigen-antibody (Ag-Ab) complexes gave more consistent results; WAG/Rij hearts were normally rejected, whereas (WAG/Rij X BN)F1 grafts survived for 13 to 16 days. Treatment of preimmunized BN rats with antidonor serum or Ag-Ab complexes after grafting did not abolish the effect of sensitization in the WAG/Rij leads to BN combination. In the (WAG/Rij X BN)F1 to BN combination, these treatments resulted in graft survival times of 4 to 13 and 11 to greater than 50 days, respectively. In most experimental groups, Ag-Ab complexes were more potent as an immunoregulatory agent than was antidonor serum.
Assuntos
Complexo Antígeno-Anticorpo , Doadores de Sangue , Sobrevivência de Enxerto , Transplante de Coração , Soros Imunes/farmacologia , Imunização , Animais , Imunidade Celular , Masculino , Ratos , Ratos Endogâmicos BN , Fatores de Tempo , Transplante HomólogoRESUMO
Various rhesus alloantisera were tested for their capacity to induce enhancement of kidney allograft survival in D locus-incompatible rhesus monkeys. Six experimental groups were investigated: 1, ten allografted monkeys remained untreated; 2, six recipients were treated with Imuran and prednisolone; 3, eight rhesus monkeys were treated with a polyspecific "anti-SD" serum; 4, four animals were given oligospecific antikidney serum; 5, four monkeys were treated with oligospecific antiblood serum; and 6, nine recipients were given anti-Ia-like sera plus immunosuppressive treatment with Imuran and prednisolone. It was found that neither treatment with Imuran and prednisolone nor the administration of any of the alloantisera had a substantial effect on kidney graft survival. However, there appeared to be a difference in renal function among experimental groups. At 6 days after grafting, the mean blood urea level in the group treated with Ia-like antisera was found to be significantly lower than those of all other groups. It is discussed that this favourable effect is attributable to treatment with anti-Ia-like sera and not to the additional factors (conventional immunosuppression and partial matching for Ia-like antigens) occurring in this experimental group.
Assuntos
Linfócitos B/imunologia , Terapia de Imunossupressão/métodos , Transplante de Rim , Macaca mulatta , Macaca , Animais , Complexo Antígeno-Anticorpo , Azatioprina/farmacologia , Sobrevivência de Enxerto/efeitos dos fármacos , Haplorrinos , Antígenos de Histocompatibilidade , Soros Imunes , Isoanticorpos , Isoantígenos , Rim/imunologia , Prednisolona/farmacologia , Transplante HomólogoAssuntos
Complexo Antígeno-Anticorpo , Sobrevivência de Enxerto , Animais , Transplante de Coração , Antígenos de Histocompatibilidade/administração & dosagem , Soros Imunes/administração & dosagem , Terapia de Imunossupressão , Injeções Intravenosas , Masculino , Ratos , Ratos Endogâmicos BN , Transplante de Pele , Transplante HomólogoRESUMO
The effects of droperidol and fentanyl on three samples of human bone marrow with regard to their colony forming in culture was studied in more than 256 multiple experiments. There was no evidence of any toxic effect of droperidol and fentanyl on the bone marrow cells.