Role of Shape in Particle-Lipid Membrane Interactions: From Surfing to Full Engulfment.

Understanding and manipulating the interactions between foreign bodies and cell membranes during endo- and phagocytosis is of paramount importance, not only for the fate of living cells but also for numerous biomedical applications. This study aims to elucidate the role of variables such as anisotropic particle shape, curvature, orientation, membrane tension, and adhesive strength in this essential process using a minimal experimental biomimetic system comprising giant unilamellar vesicles and rod-like particles with different curvatures and aspect ratios. We find that the particle wrapping process is dictated by the balance between the elastic free energy penalty and adhesion free energy gain, leading to two distinct engulfment pathways, tip-first and side-first, emphasizing the significance of the particle orientation in determining the pathway. Moreover, our experimental results are consistent with theoretical predictions in a state diagram, showcasing how to control the wrapping pathway from surfing to partial to complete wrapping by the interplay between membrane tension and adhesive strength. At moderate particle concentrations, we observed the formation of rod clusters, which exhibited cooperative and sequential wrapping. Our study contributes to a comprehensive understanding of the mechanistic intricacies of endocytosis by highlighting how the interplay between the anisotropic particle shape, curvature, orientation, membrane tension, and adhesive strength can influence the engulfment pathway.

A colloidal viewpoint on the sausage catastrophe and the finite sphere packing problem.

It is commonly believed that the most efficient way to pack a finite number of equal-sized spheres is by arranging them tightly in a cluster. However, mathematicians have conjectured that a linear arrangement may actually result in the densest packing. Here, our combined experimental and simulation study provides a physical realization of the finite sphere packing problem by studying arrangements of colloids in a flaccid lipid vesicle. We map out a state diagram displaying linear, planar, and cluster conformations of spheres, as well as bistable states which alternate between cluster-plate and plate-linear conformations due to membrane fluctuations. Finally, by systematically analyzing truncated polyhedral packings, we identify clusters of 56 ≤ N ≤ 70 number of spheres, excluding N = 57 and 63, that pack more efficiently than linear arrangements.

Charge Trapping-Based Electricity Generator (CTEG): An Ultrarobust and High Efficiency Nanogenerator for Energy Harvesting from Water Droplets.

Strategies toward harvesting energy from water movements are proposed in recent years. Reverse electrowetting allows high efficiency energy generation, but requires external electric field. Triboelectric nanogenerators, as passive energy harvesting devices, are limited by the unstable and low density of tribo-charges. Here, a charge trapping-based electricity generator (CTEG) is proposed for passive energy harvesting from water droplets with high efficiency. The hydrophobic fluoropolymer films utilized in CTEG are pre-charged by a homogeneous electrowetting-assisted charge injection (h-EWCI) method, allowing an ultrahigh negative charge density of 1.8 mC m-2 . By utilizing a dedicated designed circuit to connect the bottom electrode and top electrode of a Pt wire, instantaneous currents beyond 2 mA, power density above 160 W m-2 , and energy harvesting efficiency over 11% are achieved from continuously falling water droplets. CTEG devices show excellent robustness for energy harvesting from water drops, without appreciable degradation for intermittent testing during 100 days. These results exceed previously reported values by far. The approach is not only applicable for energy harvesting from water droplets or wave-like oscillatory fluid motion, but also opens up avenues toward other applications requiring passive electric responses, such as diverse sensors and wearable devices.