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1.
Biochem Biophys Res Commun ; 693: 149371, 2024 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-38096615

RESUMO

Adenosine diphosphate (ADP) is a nucleotide that is structurally very similar to ATP but lacks one of the two high-energy bonds due to hydrolysis. In muscle studies, ADP is usually considered exclusively as a product formed during myosin cross-bridge cycling and is not otherwise involved in this process. In our study, we question the widely held view of ADP as a final product formed during muscle contraction. Using biophysical and biochemical methods, we managed to show that ADP can act as a substrate for myosins in at least three types of muscles: smooth and striated adductor muscles of bivalves (Mytilidae and Pectinidae), and also vertebrate skeletal muscles. According to our data, the differences in the effect of ATP and ADP on the optical, biochemical, and structural properties of actomyosins are exclusively quantitative. We explain the previous ideas about ADP as a compound capable of inhibiting the ATPase activity of actomyosin by the ability of ATP and ADP to depolymerize the polymeric myosin when the concentration in the medium reaches more than 0.3 mM.


Assuntos
Trifosfato de Adenosina , Apirase , Miosinas/metabolismo , Actomiosina/metabolismo , Músculo Esquelético/metabolismo , Difosfato de Adenosina/farmacologia , Actinas/metabolismo , Cinética
2.
J Crohns Colitis ; 14(6): 818-830, 2020 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-31693091

RESUMO

BACKGROUND AND AIMS: CD39/ENTPD1 scavenges pro-inflammatory nucleotides, to ultimately generate immunosuppressive adenosine, which has a central role in immune homeostasis. Global deletion of Cd39 increases susceptibility to experimental colitis while single nucleotide polymorphisms within the human CD39 promoter, and aberrant patterns of expression during experimental hypoxia, predispose to Crohn's disease. We aimed to define the impact of transgenic human CD39 [hTG] overexpression in experimental colitis and to model therapeutic effects using the recombinant apyrase APT102 in vivo. We also determined the in vitro effects of APT102 on phenotypic and functional properties of regulatory T-lymphocytes derived from patients with Crohn's disease. METHODS: Colitis was induced by administration of dextran sulfate sodium in wild-type [WT] or hTG mice, and, in another model, by adoptive transfer of CD45RBhigh cells with or without WT or hTG regulatory T cells [Treg]. In additional experiments, mice were treated with APT102. The effects of APT102 on phenotype and function of Treg and type-1 regulatory T [Tr1] cells were also evaluated, after purification from peripheral blood and lamina propria of Crohn's disease patients [n = 38]. RESULTS: Overexpression of human CD39 attenuated experimental colitis and protected from the deleterious effects of systemic hypoxia, pharmacologically induced by deferoxamine. Administration of APT102 in vivo enhanced the beneficial effects of endogenous Cd39 boosted by the administration of the aryl hydrocarbon receptor [AhR] ligand unconjugated bilirubin [UCB]. Importantly, supplemental APT102 restored responsiveness to AhR stimulation by UCB in Treg and Tr1 cells, obtained from Crohn's disease patients. CONCLUSIONS: hCD39 overexpression ameliorated experimental colitis and prevented hypoxia-related damage in vivo. Exogenous administration of APT102 boosted AhR-mediated regulatory effects in vivo while enhancing Treg functions in Crohn's disease in vitro.


Assuntos
Antígenos CD/imunologia , Apirase/imunologia , Fatores de Transcrição Hélice-Alça-Hélice Básicos/imunologia , Doença de Crohn , Receptores de Hidrocarboneto Arílico/imunologia , Linfócitos T Reguladores/imunologia , Animais , Apirase/administração & dosagem , Doença de Crohn/imunologia , Doença de Crohn/terapia , Humanos , Imunidade Celular , Fatores Imunológicos/administração & dosagem , Fatores Imunológicos/imunologia , Camundongos
3.
Hematology ; 2(1): 39-53, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-27406725

RESUMO

Various studies, mostly done in vitro have suggested that there is interaction between platelets and polymorphonuclear leukocytes (PMNLs). Both the cells depending upon the experimental conditions release various mediators which modulate their reactivity. In the present review the effects of mediators released from PMNLs and their effect on platelet aggregation has been discussed. The present review summarizes various studies on the release of mediators such as free radicals, arachidonic acid and its metabolites, platelet activating factor, proteases, nitric oxide, ectonucleotidases and other uncharacterized factors from PMNLs. The effect of these mediators on the platelet aggregation response has also been discussed.

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