Soil bacterial community dynamics in plots managed with cover crops and no-till farming in the Lower Mississippi Alluvial Valley, USA.

AIMS: Assess bacterial community changes over time in soybean (Glycine max) crop fields following cover crop (CC) and no-till (NT) implementation under natural abiotic stressors. METHOD AND RESULTS: Soil bacterial community composition was obtained by amplifying, sequencing, and analysing the V4 region of the 16S rRNA gene. Generalized linear mixed models were used to assess the effects of tillage, CC, and time on bacterial community response. The most abundant phyla present were Acidobacteria, Actinobacteria, Bacteroidetes, and Verrucomicrobia. Bacterial diversity increased in periods with abundant water. Reduced tillage (RT) increased overall bacterial diversity, but NT with a CC was not significantly different than RT treatments under drought conditions. CCs shifted abundances of Firmicutes and Bacteroidetes depending on abiotic conditions. CONCLUSIONS: In the Lower Mississippi Alluvial Valley (LMAV), USA, NT practices lower diversity and influence long-term community changes while cover crops enact a seasonal response to environmental conditions. NT and RT management affect soil bacterial communities differently than found in other regions of the country.

Bridging gaps and seeding futures: A synthesis of soil salinization and the role of plant-soil interactions under climate change.

Soil salinization, exacerbated by climate change, poses significant threats to agricultural productivity, land restoration, and ecosystem resilience. This study reviews current knowledge on plant-soil interactions as a strategy to mitigate soil salinization induced by climate change, focusing on their role in soil salinity dynamics and tolerance mechanisms. The review examines how alterations in hydrological and temperature regimes impact soil salinity and how plant-soil mechanisms-such as salt exclusion, compartmentalization, and plant-microbe interactions-contribute to salinity mitigation. This, in turn, enhances soil quality, fertility, microbial diversity, and ecosystem services. The analysis identifies a growing body of research and highlights key themes and emerging trends, including drought, microbial communities, and salt tolerance strategies. This study underscores the critical role of plant-soil interactions in sustainable salinity management and identifies knowledge gaps and future research priorities, advocating for plant-soil interactions as a crucial pathway for improving ecosystem resilience to salinity stress amid climate change.

Soil salinization in agriculture: Mitigation and adaptation strategies combining nature-based solutions and bioengineering.

Soil salinization is among the most critical threats to agriculture and food security. Excess of salts adversely affects soil structure and fertility, plant growth, crop yield, and microorganisms. It is caused by natural processes, such as dry climates and low precipitations, high evaporation rate, poor waterlogging, and human factors, such as inappropriate irrigation practices, poor drainage systems, and excessive use of fertilizers. The growing extremization of climate with prolonged drought conditions is worsening the phenomenon. Nature-based solutions (NBS), combined with precision or conservation agriculture, represent a sustainable response, and offer benefits through revitalizing ecosystem services. This perspective explores NBS that can be adopted, along with their challenges and implementation limitations. We also argue that NBS could not be enough to combat hunger in the world's most vulnerable regions and fully achieve the Sustainable Development Goal - Zero Hunger (SDG2). We therefore discuss their possible combination with salt-tolerant crops based on bioengineering.

The soil microbiome: An essential, but neglected, component of regenerative agroecosystems.

Regenerative agriculture (RA) is gaining traction globally as an approach for meeting growing food demands while avoiding, or even remediating, the detrimental environmental consequences associated with conventional farming. Momentum is building for science to provide evidence for, or against, the putative ecosystem benefits of RA practices relative to conventional farming. In this perspective article, we advance the argument that consideration of the soil microbiome in RA research is crucial for disentangling the varied and complex relationships RA practices have with the biotic and abiotic environment, outline the expected changes in soil microbiomes under RA, and make recommendations for designing research that will answer the outstanding questions on the soil microbiome under RA. Ultimately, deeper insights into the role of microbial communities in RA soils will allow the development of biologically relevant monitoring tools which will support land managers in addressing the key environmental issues associated with agriculture.

The legacy of microbial inoculants in agroecosystems and potential for tackling climate change challenges.

Microbial inoculations contribute to reducing agricultural systems' environmental footprint by supporting sustainable production and regulating climate change. However, the indirect and cascading effects of microbial inoculants through the reshaping of soil microbiome are largely overlooked. By discussing the underlying mechanisms of plant- and soil-based microbial inoculants, we suggest that a key challenge in microbial inoculation is to understand their legacy on indigenous microbial communities and the corresponding impacts on agroecosystem functions and services relevant to climate change. We explain how these legacy effects on the soil microbiome can be understood by building on the mechanisms driving microbial invasions and placing inoculation into the context of ecological succession and community assembly. Overall, we advocate that generalizing field trials to systematically test inoculants' effectiveness and developing knowledge anchored in the scientific field of biological/microbial invasion are two essential requirements for applying microbial inoculants in agricultural ecosystems to tackle climate change challenges.

Exolaccase-boosted humification for agricultural applications.

Globally, phenolic contaminants have posed a considerable threat to agro-ecosystems. Exolaccase-boosted humification may be an admirable strategy for phenolic detoxification by creating multifunctional humic-like products (H-LPs). Nonetheless, the potential applicability of the formed H-LPs in agricultural production is still overlooked. This review describes immobilized exolaccase-enabled humification in eliminating phenolic pollutants and producing artificial H-LPs. The similarities and differences between artificial H-LPs and natural humic substances (HSs) in chemical properties are compared. In particular, the agronomic effects of these reproducible artificial H-LPs are highlighted. On the basis of the above summary, the granulation process is employed to prepare granular humic-like organic fertilizers, which can be applied to field crops by mechanical side-deep fertilization. Finally, the challenges and perspectives of exolaccase-boosted humification for practical applications are also discussed. This review is a first step toward a more profound understanding of phenolic detoxification, soil improvement, and agricultural production by exolaccase-boosted humification.

Ensuring future food security and resource sustainability: insights into the rhizosphere.

Feeding the world's growing population requires continuously increasing crop yields with less fertilizers and agrochemicals on limited land. Focusing on plant belowground traits, especially root-soil-microbe interactions, holds a great promise for overcoming this challenge. The belowground root-soil-microbe interactions are complex and involve a range of physical, chemical, and biological processes that influence nutrient-use efficiency, plant growth and health. Understanding, predicting, and manipulating these rhizosphere processes will enable us to harness the relevant interactions to improve plant productivity and nutrient-use efficiency. Here, we review the recent progress and challenges in root-soil-microbe interactions. We also highlight how root-soil-microbe interactions could be manipulated to ensure food security and resource sustainability in a changing global climate, with an emphasis on reducing our dependence on fertilizers and agrochemicals.

Aeromonas hydrophila, Bacillus thuringiensis, Escherichia coli and Pseudomonas aeruginosa utilization of Ammonium-N, Nitrate-N and Urea-N in culture.

Agricultural drainage ditches represent a major source of nutrient pollution. Shifts in nitrogen source and use of animal manures have changed the bacterial composition both in species of bacteria and their abundance in agricultural ditches. This change affects how nitrogen is being cycled and potentially the final forms of available nutrients. In particular, animal manures often have bacteria such as Escherichia coli present, increasing the abundance of a bacterial species in ditches. Research has shown that the effect of different nitrogen sources is to change bacterial community composition (class, family). How this influences the role of an individual bacterial species is poorly understood. Thus, our question was how individual species would respond to different sources of nitrogen. We used Aeromonas hydrophila, Bacillus thuringiensis, Escherichia coli and Pseudomonas aeruginosa that are common in agricultural ditches and exposed them to different concentrations of nitrogen in cultures of 1 × 100 and 1 × 10-1 dilutions from a stock solution of bacteria. Nitrogen sources were ammonium chloride, sodium nitrate and urea. The results showed A. hydrophila and E. coli have strong similarities particularly with nitrate-N and urea-N utilization and the response was often correlated with the amount of nutrient added. P. aeruginosa while similar did not show any strong correlation with amount of nutrient added. B. thuringiensis was different from the other three bacteria in utilization or production. Research has provided insight into the role of some bacteria in nitrogen cycling and may be valuable in the future to developing management strategies to reduce nutrients.

Influence of soil characteristics and leaching rate on copper migration: column test.

Soil copper (Cu) supply is used to correct Cu deficiency in many crops grown in southern Mediterranean areas. Oversupply of this fertilizer may contaminate soil and groundwater. This study aims to assess Cu migration in different soils of Moroccan agricultural areas. Three soil samples were collected from different areas. For each soil, three annual leaching rates were tested: 35 mm, 237 mm, and 565 mm for a sandy soil; 35 mm, 70 mm, and 140 mm for a clay calcareous soil; and 35 mm, 103 mm, and 247 mm for silty clay soil. The leaching experiments were conducted in columns filled identically up to 30 cm with each soil sample. Then, 0.6 mg of Cu kg-1 was added to the soil surface in the sulfate form (CuSO4.5H2O). The results showed that soil Cu loss increased mainly with leaching rate: y = 0.004 x - 0.10 (R2 = 0.97) where y: soil Cu loss (10-4 meq 100 g-1) and x: leaching rate (mm). The regression analysis did not reveal a significant influence of the soil properties on Cu leaching. However, the Cu loss seems insignificant regarding the no recurrence of high annual leaching rate (over 500 mm), in south Mediterranean areas. Also, soil Cu accumulation risk occurred in all studied soils. For sandy soil, available Cu content was 78% higher in the top layer (0-10 cm) than its content in the middle (10-20 cm) and lower (20-30 cm) layers. Furthermore, a release of bioavailable Cu was recorded in silty clay and clay calcareous soils at a leaching rate of over 70 mm.

The fertilising potential of manure-based biogas fermentation residues: pelleted vs. liquid digestate.

Spreading of manure on agricultural soils is a main source of ammonia emissions and/or nitrate leaching. It has been addressed by the European Union with the Directives 2001/81/EC and 91/676/EEC to protect the environment and the human health. The disposal of manure has therefore become an economic and environmental challenge for farmers. Thus, the conversion of manure via anaerobic digestion in a biogas plant could be a sustainable solution, having the byproducts (solid and liquid digestates) the potential to be used as fertilizers for crops. This work aimed at characterizing and assessing the effect of digestates obtained from a local biogas plant (Biogas Wipptal, Gmbh), either in the form of liquid fraction or as a solid pellet on: (i) the fertility of the soils during an incubation experiment; (ii) the plant growth and nutritional status of different species (maize and cucumber). Moreover, an extensive characterization of the pellet was performed via X-ray microanalytical techniques. The data obtained showed that both digestates exhibit a fertilizing potential for crops, depending on the plant species and the fertilizer dose: the liquid fraction increases the shoot fresh weight at low dose in cucumber, conversely, the solid pellet increases the shoot fresh weight at high dose in maize. The liquid digestate may have the advantage to release nutrients (i.e. nitrogen) more rapidly to plants, but its storage represents the main constraint (i.e. ammonia volatilization). Indeed, pelleting the digestates could improve the storability of the fertilizer besides enhancing plant nutrient availability (i.e. phosphate and potassium), plant biomass and soil biochemical quality (i.e. microbial biomass and activity). The physical structure and chemical composition of pellet digestates allow nutrients to be easily mobilized over time, representing a possible source of mineral nutrients also in long-term applications.

Assessment of soil health parameters and application of the sustainability index to fields under conservation agriculture for 3, 6, and 9 years in India.

The effect of duration of conservation agriculture adoption on soil carbon dynamics and system sustainability was evaluated on farms of 30 villages in the Nilokheri block of Karnal district, Haryana, India. Sustainability was evaluated, in which a number of soil physical, chemical, and biological parameters were measured and a Sustainability Index (SI) was applied. Soil samples were collected from existing conservation agriculture (CA) and conventional tillage (CT) farms. Villages under CA practices were subdivided as CA3, CA6, and CA9 based on the number of years of CA practice adoption. Results showed that bulk density (BD) of 0-15 cm soil depth was 7% greater in CA3 plots, whereas in CA6 and CA9 plots BD values were only 2% and 3% higher than CT. Soil organic carbon (SOC) in 0-15 cm soil depth was found to be greater by 16.32% in CA3 than CT plots, whereas SOC was higher by 38.77% and 61.22% in CA6 and CA9. In CA, for the 0-15 and 15-30 cm soil depths, labile pools were 36% and 22% greater than CT, respectively. For both the soil depths in CA, the recalcitrant pool was 12% and 9% more than CT, respectively. Microbial biomass carbon (MBC) values of the 0-15 cm soil depth were increased over CT by 18.57%, 47.08%, and 71.5% for CA3, CA6, and CA9 respectively. In CA plots, the SI of 0-15 cm soil depth ranged between cumulative ratings (CR) of 18-21, which indicates that CA practice is "sustainable" for both soil depths. For CT, CR ranged from 25 to 30 for both soil depths resulting in a SI of "sustainability with high input". Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) scores showed that SOC had the maximum weight (0.96) towards sustainability, giving it a rank of 1. Effective rooting depth (ERD), BD, texture, and wilting point (WP) ranked 2, 3, 4 and 5, respectively, indicating their corresponding weight of contribution towards the SI. Farmers in the Karnal district should be encouraged to adopt CA practices as they can increase SOC and move the systems from "sustainable with high input" to "sustainable".

Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion.

Three potential rhizobacteria namely Burkholderia gladioli (MTCC 10216), Pseudomonas sp. (MTCC 9002) and Bacillus subtilis (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek (Trigonella foenum-graecum L.) and tomato (Lycopersicon esculentum L.). Phosphate solubilizing ability of these strains individually and as consortia was tested on Pikovskayas agar medium, Phosphate solubilizing agar medium and National Botanical Research Institute phosphate agar medium containing six different sources of insoluble inorganic phosphate such as tri-calcium phosphate (TCP), di-calcium phosphate (DCP), zinc phosphate (ZP), ferric phosphate (FP), sodium di-hydrogen phosphate (SP), and aluminum phosphate (AP), and two organic P such as calcium and sodium phytate. The maximum P solubilizing ability was recorded in consortium-4 having all three potential bacterial strains. Phosphate solubilization after 7th day of incubation was 37.9 mg/100 ml of TCP, 40.01 mg/100 ml of DCP, 15.79 mg/100 ml of FP, 43.02 mg/100 ml of SP, no solubilization of ZP and AP, 39.75 mg/100 ml of calcium phytate and 24.01mg/100 ml of sodium phytate. Seed germination and the other plant parameters such as plant height and weight significantly increased in fenugreek and tomato seeds, bio-primed with consortium-4 followed by consortium-3. After bio-priming of seeds in pot assay, the level of phosphorus in soil got increased by 54% in consortium-4 treated soil followed by consortium-3 (47%) over untreated control soil. Based on these findings, consoritium-4 could be recommended as a good bio-inoculant for fenugreek, tomato and other crops in comparison to individual strains and other consortia.

Multivariate analysis for yield and yield-related traits of sesame (Sesamum indicum L.) genotypes.

Sesame production under irrigation is limited in Ethiopia because of in availability of high yielding varieties, inadequate and inefficient irrigation schemes, and insignificant awareness of producers. This study, comprising 13 sesame genotypes, was conducted around Humera and Werer during 2018 and 2019 under irrigation. The design was randomized completely block design with three replications and the objectives were to develop high yielding genotypes and identify important agronomic traits. Multivariate statistical methods like Additive Main Effect and Multiplicative Interaction (AMMI) model, Principal Component Analysis, Cluster and factor analyses were used. The genotypes (6.22%), environments (42.62) and Genotype × Environment Interactions (25.09%) were statistically (p < 0.001) significant for the agronomic traits. The grain yield in each observation varied from 383 kg/ha to 2044 kg/ha and the grand mean yield was 820.19 kg/ha. The highest mean yield was recorded from G12 (948.6 kg/ha) followed by G4 (938.9 kg/ha) while the lowest was recorded from G8 (703.1 kg/ha). G1, G4, G12, G5, G8, G11 and G13 are identified as unstable genotypes while G2, G3, G6, and G9 are stable genotypes. The genotypes were grouped in to four clusters and cluster-II was characterized as the high yielding cluster and it was also associated with grain yield, pods per plant, branches per plant and thousand seed weight. Branches per plant, pods per plant and thousand seed weight may be most determinant and crucial in developing high yielding sesame varieties. This finding recommends that G4 and G6 are desirable genotypes and can be used for irrigation production.

Evaluating potential impacts of land management practices on soil erosion in the Gilgel Abay watershed, upper Blue Nile basin.

Assessing the potential impacts of different land management practices helps to identify and implement sustainable watershed management measures. This study aims to assess a change in soil erosion rate under different land management practices in the Gilgel Abay watershed of the upper Blue Nile basin, Ethiopia. The Revised Universal Soil Loss Equation (RUSLE) model that was adapted to the Ethiopian highlands context was employed to estimate the rate of soil erosion. The impact of land management practices on soil erosion was estimated for three scenarios, which were baseline, intensive cultivation, and extensive cultivation scenarios. At the baseline scenario, the mean annual soil erosion was estimated at ~32.8 t ha-1yr-1, which is equivalent to a loss of ~13.66 Mt yr-1 from the entire watershed. While the rate of soil erosion reduced to ~11.3 t ha-1yr-1 during the implementation of intensive cultivation management practice, which reduced the total soil loss in the watershed by 65%. On the other hand, under the extensive cultivation scenario, the mean annual soil erosion rate increased to ~34.4 t ha-1yr-1. The findings suggest that implementing agricultural intensification management practices can significantly reduce soil erosion in the watershed.

Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere.

The rhizosphere offers a quintessential habitat for the microbial communities and facilitates a variety of plant-microbe interactions. Members of the genus Bacillus constitute an important group of plant growth promoting rhizobacteria (PGPR), which improve growth and yield of crops. In a total of 60 bacterial isolates from the tomato rhizosphere, 7 isolates were selected based on distinct morphological characteristics and designated as tomato rhizosphere (TRS) isolates with a number suffixed viz., TRS-1, 2, 3, 4, 5, 7, and TRS-8. All the seven isolates were Gram positive, with in vitro plant growth promoting (PGP) traits like phosphate and zinc solubilization, and also produced indoleacetic acid (IAA), phytase, siderophore, hydrogen cyanide (HCN), and 1-aminocyclopropane-1-carboxylate (ACC) deaminase, besides being antagonistic to other microbes and formed biofilm. The seven isolates belonged to the genus Bacillus as per the 16S rDNA sequence analysis. Phylogenetic tree grouped the isolates into four groups, while BOX-PCR fingerprinting allowed further differentiation of the seven isolates. The PGP activity of the isolates was measured on tomato seedlings in plant tissue culture and greenhouse assays. A significant increase in root colonization was observed over 15 days with all the isolates. Greenhouse experiments with these isolates indicated an overall increase in the growth of tomato plants, over 60 days. Isolates TRS-7 and TRS-8 were best plant growth promoters among the seven isolates, with a potential as inoculants to increase tomato productivity.

Effect of soil amendments on antioxidant activity and photosynthetic pigments in pea crops grown in arsenic contaminated soil.

The mechanism of arsenic (As) immobilization in soils is crucial for improving photosynthetic pigments and antioxidants in food crops. The effects of soil amendments with arbuscular mycorrhizal fungi (AMF), biochar (BC), selenium (Se), sulfur (S) and Si-gel on the concentrations of chlorophyll, carotenoid, proline, malondialdehyde (MDA), and the activity of ascorbate peroxidase (APX), guaiacol peroxidase (POD), and catalase (CAT) were studied in BARI pea (Pisum sativum ) under As stress. Soil amendments with AMF, Se, Si-gel and S enhanced chlorophyll a and total chlorophyll contents by 31-35% and 60-75%, respectively. Likewise, CAT activity was increased by 24-46% in BC, AMF, Se, Si-gel and S-treated pea, respectively. APX and POD activity was also found to be enriched with the treatment of BC, AMF and Se. In contrast, the content of MDA and proline was found lower than that of control in peas. These findings indicate that oxidative damage, osmotic stress and cell injury were possibly reduced in As-stressed peas. Particularly, AMF and Se both were comparatively more potential in comparison to BC. Thus, soil amendments with AMF, BC and Se are significantly important for improving antioxidant enzyme activity of food crops grown in soil with elevated As levels.

Microbial biomass carbon and enzyme activities as influenced by tillage, crop rotation and residue management in a sweet sorghum cropping system in marginal soils of South Africa.

Questions on sustainable and appropriate cropping systems for bioenergy sweet sorghum in the smallholder farming sector still exist. Therefore, a short-term experiment was carried out to study the influence of management on microbial biomass carbon (MBC), ß-glucosidase, acid phosphatase, and urease activities in a sweet sorghum cropping system in South Africa. Tillage [no-till (NT) and conventional tillage (CT)], rotation [sorghum-vetch-sorghum (S-V-S) and sorghum-fallow-sorghum (S-F-S)] and residue retention [0%, 15% and 30%] were evaluated. Tillage× rotation× residue management interaction influenced (P < 0.05) MBC whilst crop rotation residue influenced (P < 0.05) ß-glucosidase. Tillage affected ß-glucosidase (P < 0.05), acid phosphatase (P < 0.001), and urease enzyme (P < 0.01) while crop rotation only influenced acid phosphatase (P < 0.01). Residue retention affected acid phosphatase (P < 0.001) and urease enzyme (P < 0.001). NT + S-V-S+30% interaction resulted in the highest MBC content compared to CT + S-F-S+0%. NT+30% enhanced ß-glucosidase activity, S-V-S enhanced acid phosphatase compared to S-F-S. MBC and enzyme activities were positively correlated with each other. Tillage and residue management were the main factors influencing soil biological indicators under bioenergy sweet sorghum in South African marginal soils in the short-term. Soil biological indicators were higher under NT and 30% residue retention respectively. NT + S-V-S+30% was a better treatment combination to enhance soil quality under bioenergy sweet sorghum in South African marginal soils.

An investigation of the effect of biochar application rates on CO2 emissions in soils under upland rice production in southern Guinea Savannah of Nigeria.

Biochar is a chemically recalcitrant carbon-rich solid material used in soil for its potential to improve soil quality and sequester carbon. While the rate of application has implications for soil carbon dioxide (CO2) emission and the overall benefits of biochar, its effects are yet to be fully understood. To evaluate the effect of application rates of rice husk biochar on CO2 emissions, 91-day field experiments were conducted on three soil types (Anthraquic Ustorthent, Grossarenic Kandiustalf, and Ustic Quartzipsamment) in the southern Guinea Savannah of Nigeria, using three biochar application rates of 5, 15 and 25 t h-1, and control. A two-way ANOVA showed that cumulative CO2 emissions were significantly (p < 0.01) different between soil types and treatments, and soil type/treatment interactions were also significant at p = 0.05. The highest cumulative CO2-C emission of 2.77g/m2 was recorded in the Grossarenic Kandiustalf, while the least value of 2.11g/m2 was recorded in the Ustic Quartzipsamment. CO2 emission increased with increasing biochar application rates, with the highest (3.06 CO2-C g/m2) value recorded at 25 t/ha compared to 2.78 g/m2 and 1.52 g/m2 values recorded for 5 t/ha and control treatments respectively. While CO2 emissions increased with biochar application rate however, the percentage of biochar-C mineralized was higher at lower biochar rates, and differences were significant at p = 0.01. While 0.63 % of biochar C was mineralized under 5 t/ha biochar treatment, 0.15 % was recorded for 25 t/ha treatment. Factors that had significant correlation with CO2-C emission in the soils were biochar addition rate, soil pH, N, P, Ca, Mg and K. At day 91, there were no significant differences in CO2 emissions between amended treatments and control, and only a small percentage (<1) of biochar C had been mineralized.

Performance evaluation of maize hybrids in inner-plains of Nepal.

Hybridization plays a vital role in increasing the production and productivity of maize. Evaluating maize hybrids in a specific environment is a key task for the hybrid maize program. The objective of this study was to identify a promising maize hybrid for winter planting in inner terai regions. Ten maize hybrids were evaluated in a randomized complete block design (RCBD) with three replications during the winter season of 2018 and 2019 at the research field of Purwanchal Agriculture Campus, Jhapa, Nepal. The results suggested that among tested hybrids, P3396 (11.18 tons ha-1), Shresta (10.67 tons ha-1), and Rampur Hybrid 6 (10.37 tons ha-1) produced significantly higher yield in 2018 whereas P3396 (11.10 tons ha-1), Shresta (10.20 tons ha-1), and Ganga Kaveri (10.03 tons ha-1) were the ones with the highest grain yield in 2019. Comparing both years, P3396 and Shresta consistently outperform the other hybrids in terms of grain yield, which is an important traits for the farmers. Correlation studies suggested that ear weight and thousand-grain weight showed a positive significant correlation with grain yield. Therefore, we suggest P3396 and Shresta as promising hybrids for the maize growers in the inner terai regions of Nepal.

Contamination of staple crops by heavy metals in Sibaté, Colombia.

Heavy metal contamination in water resources, soil, and food sources is an issue that compromises food safety in Sibaté, Colombia. In the present study concentration of heavy metals [HMs], such as Cu, As, Pb, Cr, Zn, Co, Cd and Ni, present in vegetables included in the typical Colombian diet were measured. The study was conducted as follows: samples of parsley, artichoke and carrots produced in a location near the Muña dam were collected, where the Bogotá River water is treated for use as a water resource. To determine food safety, national and international [HMs] established limits were compared with quantified [HMs] in samples of different vegetable parts and of the surrounding soil. Fresh samples were separated in their respective parts for cold acid digestion with HCl and HNO3 (1:1) for 15 days. Heavy metal mean ± standard error (SE) were as follows (mg/kg) As 2.36 ± 0.185, Cd 0.16 ± 0.009, Co 0.43 ± 0.019, Cr 12.1 ± 0.453, Cu 13.1 ± 1.68, Ni 0.00, Pb 7.07 ± 0.482 and Zn 3.976 ± 0.332. Cd, Cr, As, Co and Ni showed high transfer factor in Cynara scolymus. Moreover, high Pb, Cu and Zn transfer factor were present in Petroselinum crispum. Except for Daucus carota roots, there was a high metal transfer specifically in Petroselinum crispum leaves and other different plant parts, with high transfer factor for Cr, As, Co, Pb, Cu and Zn.