How to become a crab: Phenotypic constraints on a recurring body plan.

A fundamental question in biology is whether phenotypes can be predicted by ecological or genomic rules. At least five cases of convergent evolution of the crab-like body plan (with a wide and flattened shape, and a bent abdomen) are known in decapod crustaceans, and have, for over 140 years, been known as "carcinization." The repeated loss of this body plan has been identified as "decarcinization." In reviewing the field, we offer phylogenetic strategies to include poorly known groups, and direct evidence from fossils, that will resolve the history of crab evolution and the degree of phenotypic variation within crabs. Proposed ecological advantages of the crab body are summarized into a hypothesis of phenotypic integration suggesting correlated evolution of the carapace shape and abdomen. Our premise provides fertile ground for future studies of the genomic and developmental basis, and the predictability, of the crab-like body form.

Osmo-ionic regulation and carbonic anhydrase, Na+/K+-ATPase and V-H+-ATPase activities in gills of the ancient freshwater crustacean Aegla schmitti (Anomura) exposed to high salinities.

Aeglidae anomuran crabs originated in the sea, but invaded and diversified in southern South American freshwater (FW) streams. We here aimed at examining their tolerance of increased salinity, after a long time of evolution in FW (~33 million years). Aegla schmitti were exposed to FW and dilute seawater of salinities 15, 20, and 25‰ for 1, 5 and 10 days. Mortality in 35‰ was also assessed. Hemolymph osmolality, Na+, K+, Cl-, and Mg2+ ions, and hydration levels of the abdominal muscle were assayed. The activities of the Carbonic Anhydrase (CA), Na+/K+-ATPase (NKA) and V-H+-ATPase (VHA) were also assayed in the gills. A. schmitti preserves osmoregulatory mechanisms of its marine ancestors. It is able to survive in high salinities (25‰) for at least 10 days. Mortality in 35‰ was of 56% after 1 day, and of 100% after 7 days. In 25‰, NaCl is apparently hyporegulated at all times, while hemolymph osmolality rises after 5 days. CA and NKA activities remained unchanged in all experimental conditions, while VHA activity decreased after 10 days in 25‰. Hemolymph NaCl data was compatible with either hyporegulation and/or putative influx of NaCl into cells for regulatory volume increase (RVI). Further studies should deepen the understanding of the roles of low permeabilities and saturation of high affinity uptake systems in truly FW decapods, in their responses to high salinities. Moreover, the fate of extracellular NaCl as secretion in true hypo-regulation and/or influx into cells for RVI should also be investigated.

ORDER within the chaos: Insights into phylogenetic relationships within the Anomura (Crustacea: Decapoda) from mitochondrial sequences and gene order rearrangements.

The infraorder Anomura consists of a morphologically and ecologically heterogeneous group of decapod crustaceans, and has attracted interest from taxonomists for decades attempting to find some order out of the seemingly chaotic diversity within the group. Species-level diversity within the Anomura runs the gamut from the "hairy" spindly-legged yeti crab found in deep-sea hydrothermal vent environments to the largest known terrestrial invertebrate, the robust coconut or robber crab. Owing to a well-developed capacity for parallel evolution, as evidenced by the occurrence of multiple independent carcinization events, Anomura has long tested the patience and skill of both taxonomists attempting to find order, and phylogeneticists trying to establish stable hypotheses of evolutionary inter-relationships. In this study, we performed genome skimming to recover the mitogenome sequences of 12 anomuran species including the world's largest extant invertebrate, the robber crab (Birgus latro), thereby over doubling these resources for this group, together with 8 new brachyuran mitogenomes. Maximum-likelihood (ML) and Bayesian-inferred (BI) phylogenetic reconstructions based on amino acid sequences from mitogenome protein-coding genes provided strong support for the monophyly of the Anomura and Brachyura and their sister relationship, consistent with previous studies. The majority of relationships within families were supported and were largely consistent with current taxonomic classifications, whereas many relationships at higher taxonomic levels were unresolved. Nevertheless, we have strong support for a polyphyletic Paguroidea and recovered a well-supported clade of a subset of paguroids (Diogenidae + Coenobitidae) basal to all other anomurans, though this requires further testing with greater taxonomic sampling. We also introduce a new feature to the MitoPhAST bioinformatics pipeline (https://github.com/mht85/MitoPhAST) that enables the extraction of mitochondrial gene order (MGO) information directly from GenBank files and clusters groups based on common MGOs. Using this tool, we compared MGOs across the Anomura and Brachyura, identifying Anomura as a taxonomic "hot spot" with high variability in MGOs among congeneric species from multiple families while noting the broad association of highly-rearranged MGOs with several anomuran lineages inhabiting extreme niches. We also demonstrate the value of MGOs as a source of novel synapomorphies for independently reinforcing tree-based relationships and for shedding light on relationships among challenging groups such as the Aegloidea and Lomisoidea that were unresolved in phylogenetic reconstructions. Overall, this study contributes a substantial amount of new genetic material for Anomura and attempts to further resolve anomuran evolutionary relationships where possible based on a combination of sequence and MGO information. The new feature in MitoPhAST adds to the relatively limited number of bioinformatics tools available for MGO analyses, which can be utilized widely across animal groups.

Taxonomic status and phylogenetic relationship of Anomura (Crustacea: Decapoda) based on mitochondrial sequences and gene order rearrangements.

The complete mitochondrial genome (mitogenome) contributed crucial information, which could improve the better understanding of molecular phylogenetic analysis, evolution, and gene rearrangements. However, only a few Coenobitidae and Albuneidae mitogenomes have been described, and controversies about the Anomuran phylogeny remain. Here, we determined three Coenobitidae species (Coenobita perlatus, Coenobita rugosus, Coenobita clypeatus) and one Albuneidae species (Emerita talpoida). As the representative species of genera or families, and even the first species of the genus, supplementing these four species is essential to study the phylogenetic relationships within Anomura. Most of the four novel mitogenomes have the typical 37 genes, except for E. talpoida, which lacked trnF. Through combining these new data with 31 Anomuran mitogenomes from Genbank, different gene rearrangement patterns and internal phylogenetic relationships of Anomura were investigated. In our phylogeny analysis, all the Anomuran species were clustered into one group, and the polyphyly of Paguroidea was well supported. Moreover, various peculiar mitochondrial gene orders (MGOs) were summarized among Anomura and preliminary determined their rearrangement mechanisms through CREx. Twenty different MGOs were suggested in our research, and we were focused seven MGO patterns (Pattern A-J) in the process of discussing the gene rearrangement mechanism. Currently making full use of the large taxon sampling, our results provide valuable information on the evolutionary status of Anomuran species and are available for systematic rearrangement and phylogenetic analyses.

The Complete Mitochondrial Genome of the Hermit Crab Diogenes edwardsii (Anomura: Diogenidae) and Phylogenetic Relationships within Infraorder Anomura.

A complete mitochondrial genome (mitogenome) can provide important information for gene rearrangement, molecular evolution and phylogenetic analysis. Currently, only a few mitogenomes of hermit crabs (superfamily Paguridae) in the infraorder Anomura have been reported. This study reports the first complete mitogenome of the hermit crab Diogenes edwardsii assembled using high-throughput sequencing. The mitogenome of Diogenes edwardsii is 19,858 bp in length and comprises 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. There are 28 and six genes observed on the heavy and light strands, respectively. The genome composition was highly A + T biased (72.16%), and exhibited a negative AT-skew (-0.110) and positive GC-skew (0.233). Phylogenetic analyses based on the nucleotide dataset of 16 Anomura species indicated that D. edwardsii was closest related to Clibanarius infraspinatus in the same family, Diogenidae. Positive selection analysis showed that two residues located in cox1 and cox2 were identified as positively selected sites with high BEB value (>95%), indicating that these two genes are under positive selection pressure. This is the first complete mitogenome of the genus Diogenes, and this finding helps us to represent a new genomic resource for hermit crab species and provide data for further evolutionary status of Diogenidae in Anomura.

Novel gene re-arrangement in the mitochondrial genome of Pisidiaserratifrons (Anomura, Galatheoidea, Porcellanidae) and phylogenetic associations in Anomura.

To improve the taxonomy and systematics of Porcellanidae within the evolution of Anomura, we describe the complete mitochondrial genomes (mitogenomes) sequence of Pisidiaserratifrons, which is 15,344 bp in size, contains the entire set of 37 genes and has an AT-rich region. Compared with the pancrustacean ground pattern, at least five gene clusters (or genes) are significantly different with the typical genes, involving eleven tRNA genes and four PCGs and the tandem duplication/random loss and recombination models were used to explain the observed large-scale gene re-arrangements. The phylogenetic results showed that all Porcellanidae species clustered together as a group with well nodal support. Most Anomura superfamilies were found to be monophyletic, except Paguroidea. Divergence time estimation implies that the age of Anomura is over 225 MYA, dating back to at least the late Triassic. Most of the extant superfamilies and families arose during the late Cretaceous to early Tertiary. In general, the results obtained in this study will contribute to a better understanding of gene re-arrangements in Porcellanidae mitogenomes and provide new insights into the phylogeny of Anomura.

Hot crabs with bold choices: Temperature has little impact on behavioural repeatability in Caribbean hermit crabs.

An animal's boldness is generally considered to be influenced by genetic and developmental factors. However, abiotic factors such as temperature have profound effects on the physiology of ectothermic animals, and thus can influence the expression and measurement of this behavioural trait. We examined the relationship between temperature and behaviour in the Caribbean hermit crab (Coenobita clypeatus) using field and lab experiments. Crabs captured in the sun were bolder than crabs captured in the shade, even when measured at a common temperature, which led to bold crabs experiencing higher microhabitat temperatures. In laboratory housed conditions, crabs demonstrated highly repeatable boldness behaviours at all temperatures, and as temperature increased, the mean behavioural latencies decreased across all individuals. Bolder crabs do not seem to rely on an innately higher thermal preference, since there was no association between boldness behaviours and thermal preference in the laboratory. Instead, bolder crabs seem to exploit more open, riskier habitats than shyer crabs. Our results highlight the complex interplay between physiological and ecological factors influencing the behaviour of a widespread and ecologically important ectothermic animal.

New records and species of deep-sea squat lobsters (Galatheoidea, Munidopsidae) from the Hawaiian Archipelago: an integrative approach using micro-CT and barcodes.

The Hawaiian Archipelago remains extensively under-sampled for many marine invertebrate taxa, including squat lobsters. During the last few years, several deep-sea expeditions carried out in the Pacific Ocean have conducted opportunistic collections of specimens and image data from the vicinity of Hawai'i. Here we describe a new species: Munidopsis hawaii sp. nov. and provide new records for Munidopsidae in the Archipelago and its associations. We illustrate and describe the new species using an integrative approach including micro-CT 3D imaging. Phylogenetic analyses of the species collected from seamounts from Hawai'i indicate that the new species represents a divergent lineage compared to morphologically similar species such as M. dispar and M. papanui. We also study the genetic distances for the species recorded in Hawai'i and other populations of the same species in the adjacent West Pacific. Three species are now known in the Hawaiian region. We also compiled identifications from images captured with ROVs in the area. These observations suggest that munidopsid species are common in the deep sea of Hawaiian waters below 1,000 m.

Two new hermit crab species of Diogenes (Crustacea: Decapoda: Diogenidae) from Atlanto-Mediterranean coasts of Iberian Peninsula: Poleward migrants or merely overlooked indigenous species?

A new hermit crab species of the genus Diogenes with reddish-orange cheliped, Diogenes erythromanus sp. nov., is described and illustrated based on specimens from the Mediterranean coasts of the Iberian Peninsula, southern Spain. In addition, a second morphotype originating from Mauritanian waters and morphologically very close to D. erythromanus sp. nov. is described as a different species, D. arguinensis sp. nov. The new species are here compared to morphologically similar congeners, especially to those inhabiting the same geographical range. Diogenes erythromanus sp. nov. is distinguishable from other Diogenes primarily by the shape and armature of the left cheliped, with a palm slightly higher than long, with a ridge of spines running along the proximal lower margin that continues with a series of spinose rows forming a central band parallel to the upper margin of the palm. The palm in D. arguinensis sp. nov. is longer than high and shows similar proximal ridge, but without central spinose ridge. The shape of the cheliped is also different in D. arguinensis sp. nov., with long dactylus, which is also flattened and twisted. Sequences from two mitochondrial and one nuclear genes, and comparative analyses with other available sequences for the genus, are also included. Molecular phylogenetic analyses support the morphological delimitation, with D. erythromanus sp. nov. and D. arguinensis sp. nov. forming a separate group, more related to other tropical species, which raises different possible explanations for its presence in the Iberian Peninsula.

De novo transcriptome assemblies of red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) molting gland and eyestalk ganglia - Temperature effects on expression of molting and growth regulatory genes in adult red king crab.

Red king crab (Paralithodes camtschaticus) and snow crab (Chionoecetes opilio) are deep-sea crustaceans widely distributed in the North Pacific and Northwest Atlantic Oceans. These giant predators have invaded the Barents Sea over the past decades, and climate-driven temperature changes may influence their distribution and abundance in the sub-Arctic region. Molting and growth in crustaceans are strongly affected by temperature, but the underlying molecular mechanisms are little known, particularly in cold-water species. Here, we describe multiple regulatory factors in the two high-latitude crabs by developing de novo transcriptomes from the molting gland (Y-organ or YO) and eye stalk ganglia (ESG), in addition to the hepatopancreas and claw muscle of red king crab. The Halloween genes encoding the ecdysteroidogenic enzymes were expressed in YO, and the ESG contained multiple neuropeptides, including molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), and ion-transport peptide (ITP). Both crabs expressed a diversity of growth-related factors, such as mTOR, AKT, Rheb and AMPKα, and stress-responsive factors, including multiple heat shock proteins (HSPs). Temperature effects on the expression of key regulatory genes were quantified by qPCR in adult red king crab males kept at 4 °C or 10 °C for two weeks during intermolt. The Halloween genes tended to be upregulated in YO at high temperature, while the ecdysteroid receptor and several growth regulators showed tissue-specific responses to elevated temperature. Constitutive and heat-inducible HSPs were expressed in an inverse temperature-dependent manner, suggesting that adult red king crabs can acclimate to increased water temperatures.

A new distribution record of Albunea groeningi (Crustacea: Anomura: Decapoda: Albuneidae) from the Digha Coast, West Bengal, India.

Members of the genus Albunea Weber, 1795 (family Albuneidae) are commonly known as sand crabs. Albuneidae contains 59 species belonging to 13 genera (Boyko McLaughlin 2010; WoRMS 2019), of which four genera and nine species are known only as fossils. Most species are relatively uncommon and adapted to living in sandy habitats (Boyko Harvey 1999). Among the 24 species of Albunea, four are known only as fossils (WoRMS 2019). The diversity and distributions of most albuneids were reported by Boyko (2002, 2010).

New occurrences of squat lobsters of the genus Eumunida Smith, 1883 (Decapoda, Eumunididae) in New Caledonia, the Solomon Islands and Papua-New Guinea, with the description of a new species.

Examination of numerous specimens of squat lobsters of the genus Eumunida Smith, 1883 collected by French cruises along the coasts of New Caledonia, the Solomon Islands and Papua-New Guinea revealed the presence of six species, including a new species. The collection data of all of these species are recorded. The new species, E. turbulenta n. sp., is described and illustrated from New Caledonia and Chesterfield Islands.

Notes on some crinoid associated decapod crustaceans (Crustacea: Decapoda) of Lakshadweep Archipelago, Central Indian Ocean.

Decapod crustaceans symbiotic with echinoderms (sea stars, brittle stars, feather stars, basket stars, sea cucumbers, and sea urchins) possess remarkable diversification in the Indo-Pacific. In the present study, seven species of decapod crustaceans symbiotic with crinoids are recorded based on collections from selected Islands (Agatti, Amini, Bangaram, Kavaratti, Kalpeni, and Minicoy) of Lakshadweep Archipelago. Of these decapods, five caridean shrimps (Alpheidae: Synalpheus carinatus (de Man, 1888), S. comatularum (Haswell, 1882), S. stimpsonii (de Man, 1888); Palaemonidae: Palaemonella pottsi (Borradaile, 1915), Pontoniopsis comanthi (Borradaile, 1915), one brachyuran crab [Pilumnidae: Permanotus purpureus (Gordon, 1934)] and one squat lobster [Galatheidae: Allogalathea elegans (Adams White, 1848)] were identified. The caridean shrimps have associated with the crinoids Comaster multifidus (Müller, 1841), Phanogenia gracilis (Hartlaub, 1893), P. distincta (Carpenter, 1888), P. multibrachiata (Carpenter, 1888) and Stephanometra indica (Smith, 1876). The brachyuran crab was observed in association with the crinoid Phanogenia gracilis whereas the squat lobsters were associated with Stephanometra tenuipinna (Hartlaub, 1890) and S. indica. Except S. stimpsonii and P. purpureus, all other species were recorded for the first time from India. Here, we provide details on morphology, distribution, habitat, and hosts for all species. We recommend further exploration of the sub-shallow coral reef areas of Lakshadweep as there will be many species that certainly remain to be discovered.

A new species of squat lobster of the genus Hendersonida (Crustacea, Decapoda, Munididae) from Papua New Guinea.

Hendersonida parvirostris sp. nov. is described from Papua New Guinea. The new species can be distinguished from the only other species of the genus, H. granulata (Henderson, 1885), by the fewer spines on the dorsal carapace surface, the shape of the rostrum and supraocular spines, the antennal peduncles, and the length of the walking legs. Pairwise genetic distances estimated using the 16S rRNA and COI DNA gene fragments indicated high levels of sequence divergence between the new species and H. granulata. Phylogenetic analyses, however, recovered both species as sister species, supporting monophyly of the genus.

Eocarcinus praecursor Withers, 1932 (Malacostraca, Decapoda, Meiura) is a stem group brachyuran.

Beginning with the description by Withers in 1932, Eocarcinus praecursor from the Jurassic has long been considered the oldest representative of the Brachyura. In 2010 Feldmann and Schweitzer re-investigated the specimens of E. praecursor and expressed doubts about the brachyuran nature of this species. Among other characters, the suspected existence of small chelae in the 2nd or 3rd pereopods led them to the conclusion that E. praecursor must be removed from the Brachyura and rather be seen as a representative of the Anomala. However, Anomala also do not possess chelae on the 2nd and 3rd pereopods. This contradiction and other aspects initiated a new investigation of E. praecursor. It can be shown that neither the 2nd nor the 3rd pereopods of E. praecursor are chelate. Furthermore, there are no other derived characters shared with anomalans. By contrast, there are a number of apomorphies shared with Brachyura such as the shape and articulation of the large chelae and the attachment points of the last two pereopods. However, not all apomorphies of the crown group are present yet. Thus, E. praecursor is a stem group representative, which allows statements about individual steps in the evolution of the set of characters of the crown group Brachyura.

A new species of the hermit crab genus Cancellus H. Milne Edwards, 1836 from a mesophotic deep bank in the northwestern Gulf of Mexico (Crustacea: Decapoda: Diogenidae).

Recent sampling on mesophotic deep banks in the northwestern Gulf of Mexico has produced a previously undescribed hermit crab assignable to the genus Cancellus H. Milne Edwards, 1836. Members of the genus are most often found to occupy cavities of eroded coral, siliceous sponges, porous calcareous rock fragments, algal concretions, or worm tubes as shelters. The present specimen was found loose as by-catch in a dredged rhodolith sample taken for algal life history studies. In situ, it likely occupied a cavity within one of the collected calcareous rhodoliths or small sponges in the by-catch. While our description is based on a single female specimen, the holotype is fully mature and intact, and it was solidly frozen in seawater until its coloration could be photographically documented and tissues extracted for sequencing. In comparison to the three other known western Atlantic species, the frontal rim of the carapace shield in the new species is continuous between the blunt lateral teeth as in C. ornatus Benedict, 1901 and C. viridis Mayo, 1873, and thus distinct from the subdivided front found in C. spongicola Benedict, 1901. The rim itself is somewhat flattened as in C. ornatus rather than inflated as in C. viridis. However, each of the ocular scales bears a pair of spines at the tip, as in C. viridis. The lower palms of the chelipeds, while distinctly rugose, do not have a separated patch of stridulating ridges comparable to those reported for C. spongicola. The yellow-orange to deep-orange pigmentation of the color pattern differs from fresh coloration in both C. ornatus and C. viridis, but that of C. spongicola is unknown for other than preserved specimens. Description of the single available specimen is in this case justified by the low likelihood for timely acquiring of additional samples from the type locality or adjacent habitats, most of which are deep banks warranting protection under pending habitat management changes. Our diagnosis includes GenBank accession numbers for COI sequences to facilitate future molecular phylogenetic comparisons.

Description of zoeal stages of Albunea paretii Guérin-Méneville, 1853 (Decapoda: Albuneidae) collected from the Amazon Continental Shelf (Brazil).

The present work describes and illustrates in detail the six zoeal stages of Albunea paretii collected from plankton samples on the Amazon continental shelf in Brazil. The morphological characters are compared with those of other known albuneid larvae. As in other albuneids, the zoeae of Albunea paretii have a semi-globose carapace, a long rostral spine and a pair of long posterolateral spines on pereon, as well as three aesthetascs on the antennule of zoea I, a strong antennal spinous process and a convex telson with a stout posterolateral spine. The zoeal morphology of Albunea paretii and other species of the genus differs, especially regarding the unique presence of the posterolateral spine only on the 5th pleonite in zoea I-IV and the absence in later zoeal stages. The differing features of the albuneid subfamilies are in the telson, antenna and maxilla format and number of setae.

Additional notes on the publication of the Narrative, Zoology and Notes from a Journal of Research into the Natural History of the Voyage of H.M.S. Samarang and its consequences for the nomenclature of decapod crustaceans and other taxa.

Captain Edward Belcher was instructed by the Lords of the Admiralty to conduct a Surveying Expedition of the various coasts and islands in the Eastern Seas using H.M.S. Samarang. During this voyage from 1843-1846, Assistant-Surgeon Arthur Adams, made a significant contribution to the collection of natural history specimens, and together with fellow officers J. Richards and W. Browne, he prepared numerous drawings used by Belcher to illustrate the Narrative of the voyage. Later, Adams collaborated with Adam White (an Assistant in the Zoological Branch of the British Museum) to describe the Samarang Crustacea, published jointly with Lovell Reeve on the Samarang Mollusca, edited the Zoology of the voyage of H.M.S. Samarang and was the author of Notes from a Journal of the Natural History which was published in the Narrative of the voyage by Belcher. In his Natural History, Adams provided detailed accounts on some of the crustaceans collected with formal descriptions of species new to science thereby making these names available. The history, nomenclature and validity of the crustacean species cited in this work is discussed and a list of the available names is tabulated.

Galathea nasimae, new name for Galathea keijii Tirmizi amp; Javed, 1993, preoccupied by Galathea keijii Karasawa, 1993 (Decapoda: Anomura: Galatheidae).

The name, Galathea keijii, was given to a Miocene fossil species of squat lobster from Japan by Karasawa (1993: 39, pl. 6, figs. 1, 2, 3, 10). In the same year, the name, Galathea keijii, was established for a living species from the Andaman Sea and Arabian Sea (Tirmizi Javed 1993: 50, fig. 22). Therefore, both names are homonymous. The description of Karasawa was published in the Bulletin of the Mizunami Fossil Museum, no. 20, which, on the inside back cover states the date of publication as December 25, 1993. The description of Tirmizi Javed was published in "Indian Ocean galatheids (Crustacea: Anomura)" by the Marine Reference Collection and Resource Centre, University of Karachi. This work was published in 1993 but the day and month of publication were not cited in the publication. In the absence of evidence to the contrary, the date of publication for "Indian Ocean galatheids (Crustacea: Anomura)" is deemed to be the last day of 1993, by application of Article 21.3.2 of the International Code of Zoological Nomenclature (ICZN 1999: 20). As such, application of Article 52 on the principle of homonymy of the International Code of Zoological Nomenclature (ICZN, 1999: 56) shows that Galathea keijii Karasawa, 1993, takes precedence over Galathea keijii Tirmizi Javed, 1993. In accordance with Article 60 on the replacement of junior homonyms (ICZN 1999: 62, 63) a new replacement name, Galathea nasimae, is here proposed for Galathea keijii Tirmizi Javed, 1993. It is dedicated to the late Dr. Nasima M. Tirmizi (Marine Reference Collection and Resource Centre, University of Karachi).

Sequencing and analysis of the complete mitochondrial genome of Coenobita brevimanus.

Coenobitidae is one of the most important families in Anomura. However, systematic classification and taxonomic studies are still limited. In this study, we determined the complete mitochondrial genome (mitogenome) of Coenobita brevimanus and further explored the phylogenetic relationships of Anomura. In the whole mitogenome of C. brevimanus (16388 bp), the AT-skew is negative and the GC-skew is positive.The phylogenetic tree was constructed using MrBayes method based on the 13 PCGs, which showed that Coenobitidae is monophyletic with maximal support value. Our phylogenetic analysis can be used to provide a basis for studies of the mitochondrial evolution of Anomura.