Potential of crop straw incorporation for replacing chemical fertilizer and reducing nutrient loss in Sichuan Province, China.

Sichuan Province is rich in crop straw, yet little is known about its spatial distribution pattern, potential in replacing chemical fertilizer and mitigating nutrient loss. Based on the statistical data and literature review, the spatial distribution and potential of nutrient resources in crop straw for replacing chemical fertilizers was evaluated in this study. The nutrient loss with both crop incorporation and chemical fertilizer application were examined using a nutrient release coefficient method and compared. Results showed that Chengdu Plain, Northeast and South Sichuan produced more than 95% of the total straw nutrient resources during the period of 2016-2020. The potential of crop straw to substitute potassium (K), nitrogen (N) and phosphorus (P) fertilizer were K2O 33.08-285.95 kg hm-2, N 9.52-82.32 kg hm-2 and P2O5 4.91-28.71 kg hm-2, respectively. If chemical fertilizer was substituted by all the available straw nutrient resources, N and P loss can be decreased by 55.12% and 65.84% in average in Sichuan Province. 343.93 t of N loss and 20.05 t of P loss can be reduced in plain areas, 122.88 t of N loss and 46.29 t of P loss can be reduced in mountainous and hilly areas, and 5.65 t of t N loss and 3.54 t of P loss can be reduced in plateau areas. It can be concluded that there were rich crop straw nutrient resources in Sichuan Province with obvious spatial variability, solid consideration should be put on to the proper use of crop straw nutrient resources, with the aim of chemical fertilizer reduction, nutrient loss reduction and sustainable development.

[Effects of Chemical Fertilizer Reduction Combined with Organic Fertilizer Application on Bacterial Community Structure in Rhizosphere/Non-Rhizosphere Soil of Lemon].

The aim of this study was to examine the effects of different fertilization methods on the physicochemical properties and bacterial community structure of lemon rhizosphere/non-rhizosphere soil in order to provide theoretical basis for scientific and rational fertilization of orchards. A pot experiment was carried out, and six fertilization treatments were set up:control (CK), conventional fertilization (FM), organic fertilizer (P), fresh organic fertilizer (NP), 70% chemical fertilizer+30% organic fertilizer (70FP), and 50% chemical fertilizer+50% organic fertilizer (50FP). Chemical analysis, real-time fluorescence quantitative PCR, and terminal restriction fragment length polymorphism (T-RFLP) were used to study the effects of different fertilization treatments on the physicochemical properties of rhizosphere and non-rhizosphere soils, the abundance of the bacterial 16S rRNA gene, and bacterial community structure. Redundancy analysis (RDA) was used to explore the environmental factors affecting the bacterial community structure of lemon rhizosphere/non-rhizosphere soil. The results showed the following:① the pH and contents of organic matter, alkali-hydrolyzed nitrogen, available phosphorus, available potassium, and nitrate nitrogen in rhizosphere/non-rhizosphere soil were significantly increased by reducing the amount of chemical fertilizer and applying organic fertilizer (50FP and 70FP) (P<0.05). Compared with conventional fertilization (FM) and single application of organic fertilizer (P and NP), the soil available P content, available K content, and nitrate nitrogen content increased by 24.76%-97.98%, 6.87%-45.11%, and 18.42%-55.82%, respectively. ② Fertilizer reduction combined with organic fertilizer significantly increased the abundance of soil bacteria and soil respiration intensity (P<0.05), and the abundance of soil rhizosphere bacteria and soil respiration intensity under the 50FP treatment increased by 15.83%-232.98% and 8.0%-162.5% compared with that under conventional fertilization and organic fertilizer alone, respectively. The bacterial abundance of rhizosphere soil was positively correlated with the pH and contents of organic matter, total nitrogen, and total phosphorus. ③ The PCoA and RDA analysis results showed that the single organic fertilizer and organic fertilizer and chemical fertilizer de-weighting of rhizosphere bacterial community structure and not adding fertilizer had a bigger difference between processing, and the main environmental factors influencing the rhizosphere/non rhizosphere bacterial community structure were organic matter, total nitrogen, total phosphorus, total potassium, alkali solution nitrogen, nitrate nitrogen, and available potassium. Fertilizer reduction combined with organic fertilizer could significantly increase soil nutrient content, increase soil bacterial abundance, and change the bacterial community structure of rhizosphere soil, and the 50FP treatment yielded better results. Therefore, 50% Chemical fertilizer+50% organic fertilizer (50FP) was a better fertilization method to improve the physical and chemical properties of orchard soil, increase the abundance of soil bacteria, and improve the soil respiration intensity.

[Effects of Chemical Fertilizer Reduction Substitute with Organic Fertilizer on Soil Functional Microbes and Lemon Yield and Quality].

To clarify the effects of non-rhizosphere/rhizosphere soil functional microbes (nitrifiers, denitrifiers, and phosphorus-solubilizing microorganisms) on lemon yield and quality, the lemon fruit and non-rhizosphere/rhizosphere soil were selected as subjects. To explore the correlation between non-rhizosphere/rhizosphere soil functional microbes and lemon yield and quality under a chemical fertilizer reduction substitute with organic fertilizer, traditional fruit quality determination and multiple molecular techniques were used. The results showed that:① 30% chemical fertilizer reduction substitute with organic fertilizer increased the nitrification intensity and phosphatase activity but effectively controlled the denitrifying enzyme activity. ② The chemical fertilizer reduction substitute with organic fertilizer significantly decreased the abundances of nitrifiers and nirS/nirK-harboring denitrifiers and significantly increased the abundances of nosZ-harboring denitrifier and phoD-harboring microorganisms. However, the diversities of functional microbial community structure did not have clear regularity under chemical fertilizer reduction substitute with organic fertilizer. ③ Compared with that under the application of chemical fertilizer and organic fertilizer alone, lemon yield and quality were the highest under the 30% reduction of chemical fertilizer substitute with organic fertilizer. ④ Nitrogen and its related microbes significantly affected lemon yield through internal and external quality. Phosphorus and its related microbes affected lemon yield mainly through internal quality. In addition, the influence factors of non-rhizosphere soil and rhizosphere soil on lemon intrinsic quality were obviously different. Altogether, these results showed that the 30% reduction of chemical fertilizer substitute with organic fertilizer significantly affected soil nitrogen and phosphorus functional microorganisms and further improved lemon yield and quality.

Effect of chemical fertilizer reduction on the quality of hybrid rice of different amylose contents.

To promote the reduction and efficiency of chemical fertilizers in rice production, two hybrid rice varieties with different amylose contents (16.43% and 27.58%) were selected to study the yield and quality performance of different quality rice varieties under reduced nitrogen/phosphorus conditions. Thus, the specific mechanism of the long-term nitrogen/phosphorus reduction effect on the quality of low- and high-amylose content (16.43% and 27.58%) rice was investigated by comparative analyses of the rapid visco analyzer and X-ray diffraction patterns, amylose contents, and starch structures of the samples. The results revealed that the effect of nitrogen fertilizer on the quality and yield of the hybrid rice was greater than that of phosphorus fertilizer. Indeed, reducing the former increased the Wx gene expression and amylose content of the rice varieties. Moreover, it reduced the starch crystallinity of Yixiangyou 1108 (2.16%), increasing the space between the starch grains and thus, chalkiness. Reducing nitrogen/phosphorus application did not significantly affect the yield and quality of the high-amylose rice but affected that of the high-quality rice with lower amylose contents. Thus, for high-amylose rice created for processing, appropriate chemical fertilizer reduction will not affect their yield and processing demand. PRACTICAL APPLICATIONS: Fertilization with different nitrogen and phosphorus fertilizers will seriously affect rice quality. The rice varieties with low amylose content (AC) could produce high taste quality rice by increasing nitrogen fertilizer and decreasing phosphorus fertilizer. The rice varieties with relatively high ACs should reduce the application of nitrogen/phosphorus fertilizer to appropriately increase AC, which can be used to produce healthy food with high resistant starch.

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A reduction of chemical fertilizers and improving fertilizer utilization rate are important for ensuring a balance between plant growth and minimizing the degradation of the black soil. We conducted a 2-year pot experiment with four treatments during 2019 and 2020, including T0: no fertilizer, T1: conventional use of chemical fertilizer, T2: 15% reduction of the chemical fertilizer combined with 400 kg·hm-2 of humic acid bio-fertilizer (HABF), and T3: 30% reduction of the chemical fertilizer combined with 600 kg·hm-2 of HABF, to examine the effect of reduction rates of chemical fertilizers combined with the HABF on soil microbial abundance, enzyme activity and nutrient content in maize cultivation. The results showed that the application of HABF significantly increased the abundance of soil bacteria and fungi, with the number of microbial colonies being positively correlated with the amount of HABF. When measured at the tassel stage of maize growth, T2 and T3 treatments significantly increased the activities of urease, sucrase, and catalase in soil by 11.4%-21.6%, 34.9%-46.7%, and 6.5%-13.4%, respectively. The available nitrogen contents in T2 and T3 treatments were higher than that in the T1 treatment by 8.2%-18.1%, which ensured the sufficient nitrogen supply to maize after the tassel stage. Soil available phosphorus and available potassium contents increased by 17.1%-121.0% and 9.6%-57.3%, respectively, compared with T1 treatment. With the increases of the amount of HABF, the activation effects of soil phosphorus and potassium and dry matter mass per plant increased significantly in T2 and T3 treatments compared with T1 treatment. In conclusion, HABF promoted the proliferation of soil bacteria and fungi, improved activities of catalase, urease, and sucrase, soil nutrient contents, and dry matter mass per plant. The 15% reduction of chemical fertilizer combined with 400 kg·hm-2 of HABF is the most suitable nutrient management strategy for maize production in black soil.

Suitable chemical fertilizer reduction mitigates the water footprint of maize production: evidence from Northeast China.

Long-term excessive use of chemical fertilizer has led to water environmental degradation. Reducing chemical fertilizer use in crop production has become a consensus, and the effects of chemical fertilizer reduction on yield, water consumption and water environment urgently need to be explored. A field experiment including four fertilization treatments: normal fertilizer application (NFA), 15%, 30% and 45% fertilizer reduction (FR) was conducted and the water footprint (WF) was used as an indicator to explore how the chemical fertilizer reduction affected the maize WF. The results showed that the blue, green and total WFs of maize in the 45% FR and NFA treatments were larger than those in the 30% FR and 15% FR treatments in 2018 and 2019. The grey WFs of maize in the NFA treatment were the highest in 2018 and 2019, exhibiting a trend that the grey WFs in the NFA treatment >15% FR treatment >30% FR treatment >45% FR treatment in 2018 and those in the NFA treatment >45% FR treatment >30% FR treatment >15% FR treatment in 2019. The optimal treatment was the 15-30% FR compared with the current fertilization, in which the total WF of maize can be minimized and the maize yield can be maximized at the same time. Precipitation had a wide-ranging impact on the yield and WF of maize, especially the amount, intensity and interval of rainfall, which had an evident impact on the grey WF. This study is expected to provide a data foundation for reducing chemical fertilizer and improving water and fertilizer use efficiency in maize production.

Commercial organic fertilizer substitution increases wheat yield by improving soil quality.

Traditional organic fertilizer substitution is an effective measure for increasing crop yield and soil quality while reducing chemical fertilizer input. However, the effects of commercial organic fertilizer substitution (COFS) on soil quality and wheat yield, and the underlying mechanisms, are unknown. In this study, agricultural fields with low fertility (LF) and high (HF) fertility soils were selected for a two-year (2018-2019) field experiment in the oasis region of Northwest China. Three fertilization treatments with three replications (no fertilization, CK; local conventional chemical fertilizer application, LCF; and 20 % of inorganic nitrogen (N) was substituted by commercial organic fertilizer, COFS) were established to study the effects of COFS on wheat growth, yield, nutrient-use efficiency and soil quality. The results showed that compared with LCF in 2018 and 2019, COFS in LF and HF promoted wheat growth, improved nitrogen use efficiency (NUE) and phosphorus use efficiency (PUE), and increased yield (by 1.52 %-3.05 % and 1.16 %-1.39 %) and soil quality (by 15.09 %-28.63 % and 22.53 %-64.82 %) by improving most soil indicators (e.g., soil organic matter (SOM) and available nutrients). Moreover, SOM and available nutrients significantly affect soil quality and wheat yield, which can monitor changes in soil quality and wheat yield. In conclusion, our study revealed that the mechanism of COFS in HF and LF increased wheat yield by improving soil quality. COFS is recommended for agricultural production, but its continuous application requires monitoring changes in SOM and available nutrients to adjust fertilization to guarantee soil quality and crop yield. This study provides guidance for the scientific application of COFS to improve farmland productivity and soil quality and helps to promote healthy and sustainable agricultural development.

Factors influencing the farmer's chemical fertilizer reduction behavior from the perspective of farmer differentiation.

Farmers are the primary decision-makers in chemical fertilizer application. Identifying their chemical fertilizer reduction behavior and its influencing factors is critical to controlling the surface source pollution caused by excessive fertilizer. This paper incorporates farmer characteristic, technology cognition and social capital into the analytical framework of farmers' fertilizer reduction behavior. Based on 889 farmers' questionnaire data, this paper constructs a Structural Equation Model to analyze farmers' fertilizer reduction behavior and its influencing factors from the perspective of farmers' differentiation. The findings are as follows. (1) Farmer characteristics, technology cognition and social capital all positively influences farmers' chemical fertilizer reduction behavior. Among them, social capital has the highest degree of influence on farmers' chemical fertilizer reduction behavior, followed by farmer characteristics, while technology cognition shows the lowest influence. (2) The effect of technology cognition on small-scale pure farmers' chemical fertilizer reduction behavior is insignificant. However, the effect on part-time farmers and large professional farmers is significant, and the effect is higher for large professional farmers. (3) The effect of farmer characteristics on part-time farmers' chemical fertilizer reduction behavior is insignificant. However, the effect on small-scale pure farmers and large professional farmers is significant, and the effect is higher for large professional farmers. (4) The effect of social capital on chemical fertilizer reduction behavior of small-scale pure farmers, part-time farmers, and large professional farmers is significant. This effect is the highest for part-time farmers, followed by large professional farmers, and the lowest for small-scale pure farmers. Accordingly, to continuously promote chemical fertilizer reduction, the government should focus on strengthening policy support, technical support, education guidance and classification.

[Impacts of Co-application of Chemical Fertilizer Reduction and Organic Material Amendment on Fluvo-aquic Soil Microbial N-cycling Functional Gene Abundances and N-converting Genetic Potentials in Northern China].

The emerging environment-associated issues due to the overuse of inorganic fertilizers in agricultural production are of global concern despite the benefit of high yields. Eco-friendly organic materials with the capability to fertilize soil are encouraged to partially replace mineral fertilizer. The N cycle conducted by soil microorganisms is the most important biogeochemical process, dictating the N bioavailability in farmland ecosystems; however, little is known about how organic material amendment affects soil microbial N cycling under chemical fertilizer reduction. Hence, a fixed field trial with five fertilization practices was implemented to experimentally alter microorganisms essential for the soil N cycle, including conventional chemical fertilization (NPK), reduced chemical fertilization (NPKR), reduced chemical fertilization plus straw (NPKRS), reduced chemical fertilization plus organic fertilizer (NPKRO), and reduced chemical fertilization plus organic fertilizer and straw (NPKROS). The microbial N-cycling gene abundances and associated N-converting genetic potentials were evaluated using real-time quantitative PCR. In comparison to conventional chemical fertilization (NPK), organic addition significantly increased the amounts of heterotrophic microbes involved in organic N decomposition, N fixation, and N reduction; however, it reduced autotrophic microbes performing ammonia oxidization. Consequently, the overall proportion of heterotrophic microbes was remarkably enhanced, and the autotrophic proportion was correspondingly lowered. The fertilization practice shift significantly improved N fixation and gaseous N emission potentials, whereas it suppressed NO3- leaching potential. A significant discrepancy among five fertilization treatments was observed based on functional gene abundances (PERMANOVA, P=0.002),as revealed by distance-based redundancy analysis (db-RDA), with NH4+ as the dominant factor. Organic fertilizer addition was beneficial for heterotrophic N functional microorganisms, with simultaneous input of straw augmenting such an effect. Pearson's correlation analysis revealed that N storage and gaseous N emission potentials were both substantially negatively correlated with NH4+; NO3- leaching potential was notably negatively associated with SOC and TN but significantly related to NH4+. In conclusion, chemical fertilizer reduction combined with organic material amendments, a main fertilization recommendation, may enhance soil N storage, diminish N loss by leaching, and mitigate the environmental risk of N2O emission. This deserves attention considering that healthy and sustainable agricultural soil environment can be cultivated from the view of microbial N-cycling.

Green Manure Amendment Can Reduce Nitrogen Fertilizer Application Rates for Oilseed Rape in Maize-Oilseed Rape Rotation.

Excessive use of chemical fertilizers has led to a reduction in the quality of arable land and environmental pollution. Using green manure to replace chemical fertilizers is one of the most effective solutions. To study the effect of green manure on the requirement for nitrogen fertilizer in oilseed rape, a field experiment with maize-oilseed rape rotation was conducted. Green manure was intercropped between rows of maize and returned after the maize harvest, with no green manure intercropped as control. Different nitrogen fertilizer treatments (0, 65%, 75% and 100% N rates, respectively) were applied during the oilseed rape season. The results showed that with a 35% reduction in nitrogen application rate, the rapeseed grain yield was significantly higher with the maize intercropping with green manure returned to the field than with the maize monocropping treatment at the same nitrogen level. Under conditions of intercropping and return of green manure, compared with the full standard rate of nitrogen fertilizer treatment, a reduction in nitrogen application of 25-30% in the rape season had no significant effect on rape yield. The agronomic efficiency of nitrogen fertilizer on oilseed rape increased significantly, by 47.61-121%, with green manure incorporation. In addition, green manure incorporation significantly increased the soil organic matter content and the soil-available nitrogen content when chemical nitrogen fertilization was abandoned. Benefit analysis showed that a 25-35% reduction in chemical nitrogen fertilizer applied to oilseed rape crops could be achieved by intercropping green manure in the maize season before the sowing of rapeseed in the experimental area. In the long-term, this measure would increase nitrogen utility, reduce production costs, and have concomitant environmental benefits of improving the quality of cultivated land.

Optimization of reduced chemical fertilizer use in tea gardens based on the assessment of related environmental and economic benefits.

Chemical fertilizer application is the primary method used to maintain tea yield and quality, but has a negative environmental impact owing to its excessive use. This study sought to assess the environmental and economic benefits of three different chemical fertilizer reduction modes: Single reduction of chemical fertilizer (SRCF), combined application of organic and chemical fertilizer (CAOF), and controlled-release fertilizer substitute (CRFS). Differences in soil nutrient content, NP (NH4+-N, NO3-N and total P) runoff loss, tea yield and quality, and the revenue of tea planting across different fertilizer reduction treatments were then discussed. We also analyzed the coupling effects of these different fertilization modes, fertilization rate and time on soil NP runoff loss, which allowed us to determine the optimum fertilization method based on differences in their respective environmental and economic benefits. Our results revealed differences in soil nutrient content, tea yield and quality, NP runoff loss, and revenue owing to tea planting across the different fertilization treatments. Soil pH after fertilization was significantly lower than before fertilization. CAOF was beneficial and improved soil nutrients as well as tea yield and quality. Of the tested methods, 50% combined application of organic and chemical fertilizer (CAOF2) was the best, as it resulted in the best tea quality and yield. CAOF2 also had the highest revenue. In addition, it was beneficial in reducing NP runoff loss. CRFS was advantageous in its persistent fertilizer efficiency and reduction in NP runoff loss. With CAOF, NP runoff loss was primarily caused by quick-acting chemical fertilizer. With extended time, NP runoff loss caused by fertilization was gradually decreased. Given our analysis of the environmental and economic benefits of different fertilizer reduction methods, CAOF2 emerged in this study as the best fertilizer reduction treatment option.