BioBlocksLab: A portable DIY Bio Lab using BioBlocks language.

Standard molecular biology laboratories are usually made with complex, sophisticated, and expensive equipment. Unfortunately, most of these labs are not affordable for everyone. In this paper, we show how we built a portable bio lab BioBlocksLab, made of four modules: a centrifuge, a thermocycler, electrophoresis, and an incubator. We also propose a new version of a blockly programming language to describe experimental lab protocols, called BioBlocks 2.0, which is based on the Microsoft MakeCode platform from the open-source project Microsoft Programming Experience Toolkit (PXT). We run BioBlocks programs of real lab protocols to control different hardware modules with biological reagents and get positive results. We offer an easy, affordable, and open-source way for everyone to do experiments with Do-It-Yourself (DIY) portable bio-labs.

Security Evaluation of Arduino Projects Developed by Hobbyist IoT Programmers.

Arduino is an open-source electronics platform based on cheap hardware and the easy-to-use software Integrated Development Environment (IDE). Nowadays, because of its open-source nature and its simple and accessible user experience, Arduino is ubiquitous and used among hobbyist and novice programmers for Do It Yourself (DIY) projects, especially in the Internet of Things (IoT) domain. Unfortunately, such diffusion comes with a price. Many developers start working on this platform without having a deep knowledge of the leading security concepts in Information and Communication Technologies (ICT). Their applications, often publicly available on GitHub (or other code-sharing platforms), can be taken as examples by other developers or downloaded and used by non-expert users, spreading these issues in other projects. For these reasons, this paper aims at understanding the current landscape by analyzing a set of open-source DIY IoT projects and looking for potential security issues. Furthermore, the paper classifies those issues according to the proper security category. This study's results offer a deeper understanding of the security concerns in Arduino projects created by hobbyist programmers and the dangers that may be faced by those who use these projects.

Miniaturized Non-Contact Heating and Transmitted Light Imaging Using an Inexpensive and Modular 3D-Printed Platform for Molecular Diagnostics.

The ability to simultaneously heat and image samples using transmitted light is crucial for several biological applications. However, existing techniques such as heated stage microscopes, thermal cyclers equipped with imaging capabilities, or non-contact heating systems are often bulky, expensive, and complex. This work presents the development and characterization of a Miniaturized Optically-clear Thermal Enclosure (MOTE) system-an open-source, inexpensive, and low-powered modular system-capable of convectively heating samples while simultaneously imaging them with transmitted light. We develop and validate a computational fluid dynamics (CFD) model to design and optimize the heating chamber. The model simulates velocity and temperature profiles within the heating chamber for various chamber materials and sizes. The computational model yielded an optimal chamber dimension capable of achieving a stable temperature ranging from ambient to 95 °C with a spatial discrepancy of less than 1.5 °C, utilizing less than 8.5 W of power. The dual-functionality of the MOTE system, enabling synchronous heating and transmitted light imaging, was demonstrated through the successful execution of paper-based LAMP reactions to detect λ DNA samples in real-time down to 10 copies/µL of the target concentration. The MOTE system offers a promising and flexible platform for various applications, from molecular diagnostics to biochemical analyses, cell biology, genomics, and education.

A review of homemade cosmetics based on a study of 150 blogs and their authors.

Do-it-yourself methods have been used in many fields (cooking, home improvement, decoration, gardening, etc.) for a long time, but their application to the cosmetics sector is relatively recent and seems to be linked to a number of health scandals. The objective of this work is to analyse homemade cosmetics based on a study of blogs and their authors. We made a study of 150 blogs advocating homemade cosmetics. With only one exception, the blog authors were women, mostly in their thirties, with no specific qualifications or knowledge in the fields of cosmetic formulation or chemistry. The most highly qualified of them (with at least a Master's degree) had studied marketing and management. So, the situation is an illustration of the Dunning-Kruger effect, with authors believing themselves to be qualified in a field wholly outside their own. This leads to scientific falsehoods about, for example, preservatives like parabens and phenoxyethanol. On the contrary, the relevance of the ecological motivation frequently mentioned in these blogs is undeniable.

L'étude de 150 blogs prônant le DIY cosmétique nous a permis de mettre en évidence, qu'à une exception près, les auteurs étaient des femmes, des trentenaires pour la plupart, sans qualification particulière ni connaissance dans le domaine de la formulation cosmétique, ni dans celui de la chimie. Les plus diplômées (niveau Master minimum) ont suivi des formations orientées vers le marketing et le management. Nous avons donc ici une illustration de l'effet Dunning-Kruger, les auteurs s'estimant qualifiées dans un domaine qui n'est pas du tout le leur. Cela se traduit par des contre-vérités scientifiques sur les conservateurs, les parabens et le phénoxyéthanol, par exemple. Il ne faut, en revanche, pas nier la pertinence des motivations écologiques qui sont fréquemment évoquées dans ces blogs.

Development of a plant conveyance system using an AGV and a self-designed plant-handling device: A case study of DIY plant phenotyping.

Plant phenotyping technology has been actively developed in recent years, but the introduction of these technologies into the field of agronomic research has not progressed as expected, in part due to the need for flexibility and low cost. "DIY" (Do It Yourself) methodologies are an efficient way to overcome such obstacles. Devices with modular functionality are critical to DIY experimentation, allowing researchers flexibility of design. In this study, we developed a plant conveyance system using a commercial AGV (Automated Guided Vehicle) as a case study of DIY plant phenotyping. The convey module consists of two devices, a running device and a plant-handling device. The running device was developed based on a commercial AGV Kit. The plant-handling device, plant stands, and pot attachments were originally designed and fabricated by us and our associates. Software was also developed for connecting the devices and operating the system. The run route was set with magnetic tape, which can be easily changed or rerouted. Our plant delivery system was developed with low cost and having high flexibility, as a unit that can contribute to others' DIY' plant research efforts as well as our own. It is expected that the developed devices will contribute to diverse phenotype observations of plants in the greenhouse as well as to other important functions in plant breeding and agricultural production.

Hardware Selection and Performance of Low-Cost Fluorometers.

Access to and extensive use of fluorometric analyses is limited, despite its extensive utility in environmental transport and fate. Wide-spread application of fluorescent tracers has been limited by the prohibitive costs of research-grade equipment and logistical constraints of sampling, due to the need for high spatial resolutions and access to remote locations over long timescales. Recently, low-cost alternatives to research-grade equipment have been found to produce comparable data at a small fraction of the price for commercial equipment. Here, we prototyped and benchmarked performance of a variety of fluorometer components against commercial units, including performance as a function of tracer concentration, turbidity, and temperature, all of which are known to impact fluorometer performance. While component performance was found to be comparable to the commercial units tested, the best configuration tested obtained a functional resolution of 0.1 ppb, a working concentration range of 0.1 to >300 ppb, and a cost of USD 59.13.

Product hacking as a systematic intervention: towards new strategies and platforms in design education.

This paper is mainly about how product hacking can be categorized and implemented in a systematic way in design education. The theoretical or analytical part of the project is based on a study in which a great number of hacking projects are reviewed and categorized found in popular DIY platforms. The implementation or synthesis part of the research is a design project held in an industrial design department with freshman design students based on the categorization of function proposed, which are namely; enhance, change and add function. In this project students are required to make three kinds of interventions on functions of existing products, also share their projects in the selected platform (www.instructables.com), so that other people can find, criticize, develop, or apply it by using the instructions given. Sharing in platforms like these creates a connection between the analytical and synthetic phases of the paper, and also makes it different from other classical projects confined to a closed studio atmosphere.This paper also seeks for the possibility of fresh perspectives on issues like sustainability, ready-made and open source design by engaging students in an intervention-based design process where they look for formulating their designs in a methodological way.One other aspect of the project was the idea that it has the potential to create an advantage or value for the time and context it was given, that is when the COVID-19 has started to influence our lives deeply.

A real-world study of user characteristics, safety and efficacy of open-source closed-loop systems and Medtronic 670G.

We report a real-world evaluation of the first commercially approved automated insulin delivery (AID) system, MiniMed 670G (670G), and open source-automated insulin delivery (OS-AID) systems. This was undertaken as a retrospective observational study in adults with type 1 diabetes using AID systems for 6 months or longer in a publicly funded health service using clinically validated data. Sixty-eight adults (38 670G, 30 OS-AID systems) were included. OS-AID system users were younger, had a shorter diabetes duration and a higher education status. OS-AID systems displayed a significantly better change in HbA1c (median -0.9% [-0.4%, -1.1%] vs. -0.1% [IQR -0.7%, 0.2%], P = .004) and time in range 3.9-10 mmol/L (mean 78.5%, SD ± 12.0% vs. 68.2% ± 14.7%, P = .024) compared with 670G. Both systems showed minimal hypoglycaemia, with OS-AID systems revealing significantly improved secondary outcomes of mean glucose and percentage of time more than 10 mmol/L, with a higher percentage of time of less than 3 mmol/L. OS-AID system users displayed improved glycaemic outcomes with no clinical safety concerns compared with 670G, although higher weight-adjusted insulin dose and weight gain were noted. The study highlights key differences in OS-AID system user characteristics that are important for interpreting real-world findings from recent OS-AID system studies.

An Open-Source, Durable, and Low-Cost Alternative to Commercially Available Soil Temperature Data Loggers.

Soil temperatures play an important role in determining the distribution and function of organisms. However, soil temperature is decoupled from air temperature and varies widely in space. Characterizing and predicting soil temperature requires large and expensive networks of data loggers. We developed an open-source soil temperature data logger and created online resources to ensure our design was accessible. We tested data loggers constructed by students, with little prior electronics experience, in the lab, and in the field in Alaska. The do-it-yourself (DIY) data logger was comparably accurate to a commercial system with a mean absolute error of 2% from -20-0 °C and 1% from 0-20 °C. They captured accurate soil temperature data and performed reliably in the field with less than 10% failing in the first year of deployment. The DIY loggers were ~1.7-7 times less expensive than commercial systems. This work has the potential to increase the spatial resolution of soil temperature monitoring and serve as a powerful educational tool. The DIY soil temperature data logger will reduce data collection costs and improve our understanding of species distributions and ecological processes. It also provides an educational resource to enhance STEM, accessibility, inclusivity, and engagement.

Deaths and Severe Adverse Events after the use of Mifepristone as an Abortifacient from September 2000 to February 2019.

OBJECTIVES: Primary: Analyze the Adverse Events (AEs) reported to the Food and Drug Administration (FDA) after use of mifepristone as an abortifacient. Secondary: Analyze maternal intent after ongoing pregnancy and investigate hemorrhage after mifepristone alone. METHODS: Adverse Event Reports (AERs) for mifepristone used as an abortifacient, submitted to the FDA from September 2000 to February 2019, were analyzed using the National Cancer Institute's Common Terminology Criteria for Adverse Events (CTCAEv3). RESULTS: The FDA provided 6158 pages of AERs. Duplicates, non-US, or AERs previously published (Gary, 2006) were excluded. Of the remaining, there were 3197 unique, US-only AERs of which there were 537 (16.80%) with insufficient information to determine clinical severity, leaving 2660 (83.20%) Codable US AERs. (Figure 1). Of these, 20 were Deaths, 529 were Life-threatening, 1957 were Severe, 151 were Moderate, and 3 were Mild.The deaths included: 9 (45.00%) sepsis, 4 (20.00%) drug toxicity/overdose, 1 (5.00%) ruptured ectopic pregnancy, 1 (5.00%) hemorrhage, 3 (15.00%) possible homicides, 1 (5.00%) suicide, 1 (5.00%) unknown. (Table 1).Retained products of conception and hemorrhage caused most morbidity. There were 75 ectopic pregnancies, including 26 ruptured ectopics (includes one death).There were 2243 surgeries including 2146 (95.68%) D&Cs of which only 853 (39.75%) were performed by abortion providers.Of 452 patients with ongoing pregnancies, 102 (22.57%) chose to keep their baby, 148 (32.74%) had terminations, 1 (0.22%) miscarried, and 201 (44.47%) had unknown outcomes.Hemorrhage occurred more often in those who took mifepristone and misoprostol (51.44%) than in those who took mifepristone alone (22.41%). CONCLUSIONS: Significant morbidity and mortality have occurred following the use of mifepristone as an abortifacient. A pre-abortion ultrasound should be required to rule out ectopic pregnancy and confirm gestational age. The FDA AER system is inadequate and significantly underestimates the adverse events from mifepristone.A mandatory registry of ongoing pregnancies is essential considering the number of ongoing pregnancies especially considering the known teratogenicity of misoprostol.The decision to prevent the FDA from enforcing REMS during the COVID-19 pandemic needs to be reversed and REMS must be strengthened.

DIY gerontechnology: circumventing mismatched technologies and bureaucratic procedure by creating care technologies of one's own.

This study analyses 'Do-It-Yourself' (DIY) gerontechnologies and shows that they can be viable and valuable alternatives to 'ready-made' gerontechnologies. Using the concept of innosumption, we analyze the work of care workers in gerontechnology showrooms in Norway. We show how and why care workers will sometimes advice older adults to assemble DIY-gerontechnologies. Such DIY-gerontechnologies are not high-tech solutions made by technology producers, but creative solutions that older adults' suit to their specific needs and assemble for themselves from mundane objects that are available in shops. So far, analyses of the design, implementation and use of gerontechnologies have almost exclusively focused on professionally designed and produced 'ready-made' gerontechnologies. But for various reasons, ready-made gerontechnologies often do not fit in well with the lives of older people. In such cases, care workers guide older people to the innosumption of DIY-gerontechnologies that offer workable solutions that are useful, quickly implemented, easily understandable and often cheap. We show that and how the existence of DIY-gerontechnologies questions the reasons behind the strong and widely accepted assumption that only high-tech innovations are a proper solution to the needs of older people.

A DIY Low-Cost Wireless Wind Data Acquisition System Used to Study an Arid Coastal Foredune.

Environmental studies on coastal dune systems are faced with a considerable cost barrier due to the cost of the instrumentation and sensory equipment required for data collection. These systems play an important role in coastal areas as a protection against erosion and as providers of stability to coastal sedimentary deposits. The DIY (Do-It-Yourself) approach to data acquisition can reduce the cost of these environmental studies. In this paper, a low-cost DIY wireless wind data acquisition system is presented which reduces the cost barrier inherent to these types of studies. The system is deployed for the analysis of the foredune of Maspalomas, an arid dune field situated on the south coast of Gran Canaria (Canary Islands, Spain), for the specific purpose of studying the dynamics of a dune type (tongue dunes), which is typical of this environment. The results obtained can be of interest for the study of these coastal environments at both the local level, for the management of this particular dune field, and at the general level for other similar dune fields around the world.

[Effect of using the nightscout system on metabolic control, safety, and incidence of complications in patients with diabetes mellitus].

OBJECTIVE: Introduction: The Nightscout system is a free Do It Yourself solution. This system appeared in Poland in 2016. The concept of the project is to provide insight into measurements from the system of continuous glucose monitoring in interstitial fluid by authorized persons. The aim: The study was carried out to assess the functionality of the Nightscout system and its effect onmetabolic control, safety and the incidence of complications in patients with type 1 diabetes mellitus. PATIENTS AND METHODS: Material and methods:The study comprised 98 patients with type 1 diabetes. The study was conducted online using an anonymous questionnaire targeted at members of the "Nightscout Polska (Poland)" group on the Facebook. RESULTS: Results: Severe hypoglycaemic episodes with loss of consciousness were more frequent before using the Nightscout system and were reported by 3 adults (1-2 episodes). No severe hypoglycaemic episodes were reported when using the Nightscout system. The number of ketoacidosis episodes was reduced from 5 before using the Nightscout system to 2 episodes during the use of the system in children, and from 3 to 0 episodes in adults. Levels of glycated haemoglobin were lower in patients using the Nightscout system, both compared to control groups and values before the use it. In people using the Nightscout system glycated hemoglobin values were lower than their values before using this system. It was also shown that people using the Nightscout system had lower glycated hemoglobin than people from the control group. CONCLUSION: Conclusions: Using the Nightscout system can positively affect the safety of insulin therapy and the treatment process of type 1 diabetes. The Nightscout system can be an improvement of the system of continuous glucose monitoring in interstitial fluid.

A Low-Cost, Multi-Sensor System to Monitor Temporary Stream Dynamics in Mountainous Headwater Catchments.

While temporary streams account for more than half of the global discharge, high spatiotemporal resolution data on the three main hydrological states (dry streambed, standing water, and flowing water) of temporary stream remains sparse. This study presents a low-cost, multi-sensor system to monitor the hydrological state of temporary streams in mountainous headwaters. The monitoring system consists of an Arduino microcontroller board combined with an SD-card data logger shield, and four sensors: an electrical resistance (ER) sensor, temperature sensor, float switch sensor, and flow sensor. The monitoring system was tested in a small mountainous headwater catchment, where it was installed on multiple locations in the stream network, during two field seasons (2016 and 2017). Time-lapse cameras were installed at all monitoring system locations to evaluate the sensor performance. The field tests showed that the monitoring system was power efficient (running for nine months on four AA batteries at a five-minute logging interval) and able to reliably log data (<1% failed data logs). Of the sensors, the ER sensor (99.9% correct state data and 90.9% correctly timed state changes) and flow sensor (99.9% correct state data and 90.5% correctly timed state changes) performed best (2017 performance results). A setup of the monitoring system with these sensors can provide long-term, high spatiotemporal resolution data on the hydrological state of temporary streams, which will help to improve our understanding of the hydrological functioning of these important systems.

Seeing power with a flashlight: DIY thermal sensing technology in the classroom.

This paper contributes to the growing literature on 'making and doing' in Science and Technology Studies (STS) by describing and theorizing the teaching of making and doing. We describe a collaborative do-it-yourself (DIY) technology project taught simultaneously in Canada and the United States, in sociology and public health, to undergraduates with no prior electronics experience. Students built thermal flashlights - low cost digital tools for making thermal images - and employed them to research their surrounding environments. By making and using the thermal flashlights, learners investigated power in two senses: identifying social power relationships embedded within normally unquestioned infrastructures, and exploring these infrastructures' connection to industrial forms of power, such as heat and electricity. Students and instructors came to understand how the control of power, light and temperature is vital to human-made infrastructure and environmental health threats that characterize the 21st century. Through this project, students went from being passive consumers of such power to become active investigators of their socio-technical systems by producing unique knowledge that enabled them to imagine how they might make and inhabit their environments differently. Breaking down the distinction between teaching and research, this article explores the promise of 'making and doing' in university courses to create new collaborative research platforms that could spread laterally and scale to transform social and technical infrastructures.

3D Printing Neuron Equivalent Circuits: An Undergraduate Laboratory Exercise.

The electrical equivalent circuit for a neuron is composed of common electrical components in a configuration that replicates the passive electrical properties and behaviors of the neural membrane. It is a powerful tool used to derive such fundamental neurophysiological equations as the Hodgkin-Huxley equations, and it is also the basis for well-known exercises that help students to model the passive (Ohmic) properties of the neuronal membrane. Unfortunately, as these exercises require basic knowledge of electronics, they are generally not physically conducted in biomedical courses, but remain merely conceptual exercises in a book or simulations on a computer. In such manifestations, they lack the "hands-on" appeal for students and teachers afforded by laboratory experimentations. Here, we propose a new approach to these experiments in which a desktop 3D printer and conductive paint are used to build the circuit and the popular programmable microcontroller, the Arduino UNO, is used as a graphical oscilloscope when connected to a standard computer. This set-up has the advantage of being very easy to build and less clumsy than a circuit in a prototyping board or connected with alligator clips, with the added benefit of being conveniently portable for classroom demonstrations. Most importantly, this method allows the monitoring of real-time changes in the current flowing through the circuit by means of a graphical display (by way of the Arduino) at a fraction of the cost of commercially available oscilloscopes.

A Low-Cost Environmental Monitoring System: How to Prevent Systematic Errors in the Design Phase through the Combined Use of Additive Manufacturing and Thermographic Techniques.

nEMoS (nano Environmental Monitoring System) is a 3D-printed device built following the Do-It-Yourself (DIY) approach. It can be connected to the web and it can be used to assess indoor environmental quality (IEQ). It is built using some low-cost sensors connected to an Arduino microcontroller board. The device is assembled in a small-sized case and both thermohygrometric sensors used to measure the air temperature and relative humidity, and the globe thermometer used to measure the radiant temperature, can be subject to thermal effects due to overheating of some nearby components. A thermographic analysis was made to rule out this possibility. The paper shows how the pervasive technique of additive manufacturing can be combined with the more traditional thermographic techniques to redesign the case and to verify the accuracy of the optimized system in order to prevent instrumental systematic errors in terms of the difference between experimental and actual values of the above-mentioned environmental parameters.

Design and Development of a Nearable Wireless System to Control Indoor Air Quality and Indoor Lighting Quality.

The article describes the results of the project "open source smart lamp" aimed at designing and developing a smart object able to manage and control the indoor environmental quality (IEQ) of the built environment. A first version of this smart object, built following a do-it-yourself (DIY) approach using a microcontroller, an integrated temperature and relative humidity sensor, and techniques of additive manufacturing, allows the adjustment of the indoor thermal comfort quality (ICQ), by interacting directly with the air conditioner. As is well known, the IEQ is a holistic concept including indoor air quality (IAQ), indoor lighting quality (ILQ) and acoustic comfort, besides thermal comfort. The upgrade of the smart lamp bridges the gap of the first version of the device providing the possibility of interaction with the air exchange unit and lighting system in order to get an overview of the potential of a nearable device in the management of the IEQ. The upgraded version was tested in a real office equipped with mechanical ventilation and an air conditioning system. This office was occupied by four workers. The experiment is compared with a baseline scenario and the results show how the application of the nearable device effectively optimizes both IAQ and ILQ.

Grasshopper DCMD: An Undergraduate Electrophysiology Lab for Investigating Single-Unit Responses to Behaviorally-Relevant Stimuli.

Avoiding capture from a fast-approaching predator is an important survival skill shared by many animals. Investigating the neural circuits that give rise to this escape behavior can provide a tractable demonstration of systems-level neuroscience research for undergraduate laboratories. In this paper, we describe three related hands-on exercises using the grasshopper and affordable technology to bring neurophysiology, neuroethology, and neural computation to life and enhance student understanding and interest. We simplified a looming stimuli procedure using the Backyard Brains SpikerBox bioamplifier, an open-source and low-cost electrophysiology rig, to extracellularly record activity of the descending contralateral movement detector (DCMD) neuron from the grasshopper's neck. The DCMD activity underlies the grasshopper's motor responses to looming monocular visual cues and can easily be recorded and analyzed on an open-source iOS oscilloscope app, Spike Recorder. Visual stimuli are presented to the grasshopper by this same mobile application allowing for synchronized recording of stimuli and neural activity. An in-app spike-sorting algorithm is described that allows a quick way for students to record, sort, and analyze their data at the bench. We also describe a way for students to export these data to other analysis tools. With the protocol described, students will be able to prepare the grasshopper, find and record from the DCMD neuron, and visualize the DCMD responses to quantitatively investigate the escape system by adjusting the speed and size of simulated approaching objects. We describe the results from 22 grasshoppers, where 50 of the 57 recording sessions (87.7%) had a reliable DCMD response. Finally, we field-tested our experiment in an undergraduate neuroscience laboratory and found that a majority of students (67%) could perform this exercise in one two-hour lab setting, and had an increase in interest for studying the neural systems that drive behavior.

Synthetic biology: from mainstream to counterculture.

Existing at the interface of science and engineering, synthetic biology represents a new and emerging field of mainstream biology. However, there also exists a counterculture of Do-It-Yourself biologists, citizen scientists, who have made significant inroads, particularly in the design and development of new tools and techniques. Herein, I review the development and convergence of synthetic biology's mainstream and countercultures.