RESUMO
The research examined the impact of an ethanolic extract from the leaves of Kratom (Mitragyna speciosa (Korth.) Havil.) on the growth, antioxidant capacity, immune-related gene expression, and resistance to disease caused by Edwardsiella tarda in Nile tilapia (Oreochromis niloticus). The findings revealed that the extract had the important phytochemical content in the extract included total phenolics content, total flavonoids content, vitamin C, and total antioxidant capacity and 5.42 % of the crude extract was mitragynine. The extract demonstrated antioxidant activity, as evidenced by its IC50 values against ABTS and DPPH radicals and its ferric reducing power in vitro. Moreover, the MIC-IC50 value of 0.625 mg/mL indicated that the growth of the bacteria was reduced by approximately 50 %, and the MBC was 2.50 mg/mL against E. tarda. Furthermore, the orally administered Kratom leaf extract to fingerling tilapia for 8 weeks exhibited a noticeable increase in oxidative stress, as demonstrated by the increase in MDA production in the 10 and 25 g/kg groups. It also exhibited an increase in acetylcholinesterase (AChE) activity in muscle tissue at the 50 g/kg group. However, when administered at a feeding rate of 5-10 g/kg feed, the extract showed an increase in the expression of immune-related genes (IL1, IL6, IL8, NF-kB, IFNγ, TNFα, Mx, CC-chemokine, CD4, TCRß, MHC-IIß, IgM, IgT, IgD) and enhanced resistance to E. tarda infection in fish. Conversely, administering the extract at 25-50 g/kg feed resulted in contrasting effects, suppressing and reducing the observed parameters. Nevertheless, feeding the extract at all concentrations for 8 weeks did not produce any changes in the histology or systemic functioning of the liver and intestines, as indicated by blood biochemistry. These findings suggest that the ethanolic leaf extract from Kratom has the potential to be used as a substitute for antibiotics in the management of bacterial infections in Nile tilapia culture, with a recommended dosage of 5-10 g/kg feed/day for a maximum of 8 weeks.
Assuntos
Antibacterianos , Antioxidantes , Ciclídeos , Edwardsiella tarda , Infecções por Enterobacteriaceae , Doenças dos Peixes , Mitragyna , Extratos Vegetais , Folhas de Planta , Animais , Doenças dos Peixes/imunologia , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Extratos Vegetais/administração & dosagem , Ciclídeos/imunologia , Ciclídeos/crescimento & desenvolvimento , Edwardsiella tarda/efeitos dos fármacos , Edwardsiella tarda/fisiologia , Infecções por Enterobacteriaceae/veterinária , Infecções por Enterobacteriaceae/imunologia , Antioxidantes/farmacologia , Folhas de Planta/química , Antibacterianos/farmacologia , Mitragyna/química , Resistência à Doença/efeitos dos fármacos , Dieta/veterinária , Ração Animal/análise , Suplementos Nutricionais/análiseRESUMO
Interferon regulatory factor (IRF) family involves in the transcriptional regulation of type I Interferons (IFNs) and IFN-stimulated genes (ISGs) and plays a critical role in cytokine signaling and immune response. However, systematic identification of the IRF gene family in teleost has been rarely reported. In this study, twelve IRF members, named PoIRF1, PoIRF2, PoIRF3, PoIRF4a, PoIRF4b, PoIRF5, PoIRF6, PoIRF7, PoIRF8, PoIRF9, PoIRF10 and PoIRF11, were identified from genome-wide data of olive flounder (Paralichthys olivaceus). Phylogenetic analysis indicated that PoIRFs could be classified into four clades, including IRF1 subfamily (PoIRF1, PoIRF11), IRF3 subfamily (PoIRF3, PoIRF7), IRF4 subfamily (PoIRF4a, PoIRF8, PoIRF9, PoIRF10) and IRF5 subfamily (PoIRF5, PoIRF6). They were evolutionarily related to their counterparts in other fish. Gene structure and motif analysis showed that PoIRFs protein sequences were highly conserved. Under normal physiological conditions, all PoIRFs were generally expressed in multiple developmental stages and healthy tissues. After E. tarda attack and temperature stress, twelve PoIRFs showed significant and different changes in mRNA levels. The expression of PoIRF1, PoIRF3, PoIRF4a, PoIRF5, PoIRF7, PoIRF8, PoIRF9, PoIRF10 and PoIRF11 could be markedly induced by E. tarda, indicating that they played a key role in the process of antibacterial immunity. Besides, temperature stress could significantly stimulate the expression of PoIRF3, PoIRF5, PoIRF6 and PoIRF7, indicating that they could transmit signals rapidly when the temperature changes. In conclusion, this study reported the molecular properties and expression analysis of PoIRFs, and explored their role in immune response, which laid a favorable foundation for further studies on the evolution and functional characteristics of the IRF family in teleost fish.
Assuntos
Infecções por Enterobacteriaceae , Doenças dos Peixes , Linguado , Animais , Edwardsiella tarda , Filogenia , Temperatura , Fatores Reguladores de Interferon/genética , Fatores Reguladores de Interferon/metabolismo , Infecções por Enterobacteriaceae/veterináriaRESUMO
The suppressors of cytokine signaling (SOCS) gene family participates in development and immunity through negative regulation of cytokine signaling pathways. Although the immune response of SOCS gene family members has been extensively characterized in teleost, no similar study has been reported in olive flounder yet. In our present study, a total of 13 SOCSs in olive flounder were identified and characterized systematically. By querying the SOCS sequences of ten teleost fish species, we found there were exactly more members of SOCSs in fish than mammals, which indicated that there were more duplication events occurred in fish than in higher vertebrates. Phylogenetic analysis clearly illuminated that SOCS genes were highly conserved. The analysis of gene structure and motif showed SOCS proteins of olive flounder shared a high level of sequence similarity strikingly. The expression profiles of tissues and developmental stages indicated that SOCS members had a kind of specificity in temporality and spatiality. RNA-Seq analysis of temperature stress and E. Tarda infection demonstrated SOCS members were involved in inflammatory response. In a word, our results would provide a further reference for understanding the mechanism of SOCS genes in olive flounder.
Assuntos
Infecções por Enterobacteriaceae , Doenças dos Peixes , Linguado , Animais , Linguado/genética , Edwardsiella tarda/fisiologia , Temperatura , Filogenia , Imunidade , Citocinas/genética , Infecções por Enterobacteriaceae/veterinária , MamíferosRESUMO
The signal transducer and activator of transcription (STAT) family members are not only the transcriptional activators, but also play important roles in regulating inflammatory response. Some members have been reported to be involved in innate bacterial and antiviral immunity in aquatic organisms. However, no systematic research on STATs has been found in teleost. In this present study, we characterized six STAT genes in Japanese flounder based on bioinformatics methods, namely PoSTAT1, PoSTAT2, PoSTAT3, PoSTAT4, PoSTAT5 and PoSTAT6. The phylogenetic analysis of STATs in fish indicated that STATs were highly conserved and revealed an absence of STAT5 in a few species. Further analysis of gene structures and motifs showed STAT proteins shared a similar structure and probably had similar functionality in Japanese flounder. The expression profiles of different development stages and tissues demonstrated that PoSTATs exhibited specificity in temporality and spatiality as well as PoSTAT4 was highly expressed in gill. The transcriptome data analysis of E. tarda and temperature stress showed that PoSTAT1 and PoSTAT2 were more respective to these two kinds of stress. In addition, the results also demonstrated that these PoSTATs might regulate immune response in different ways, manifested by up-regulation in E. tarda infection and down-regulation in temperature stress. In a word, this systematic analysis of PoSTATs would provide valuable information about the phylogenetic relationship of STATs in fish species and help understand the role of STAT genes in the immune response of Japanese flounder.
Assuntos
Infecções por Enterobacteriaceae , Doenças dos Peixes , Linguado , Animais , Imunidade Inata/genética , Edwardsiella tarda/fisiologia , Temperatura , Filogenia , Proteínas de Peixes/química , Infecções por Enterobacteriaceae/genética , Infecções por Enterobacteriaceae/veterinária , Regulação da Expressão GênicaRESUMO
Toll-like receptor (TLR) genes are best known for their roles in the innate immune defense. However, studies focusing on the reaction mechanisms of TLR genes in olive flounder (Paralichthys olivaceus) immune responses are still limited. In this study, 11 TLR family members (PoTLRs) were identified and classified from P. olivaceus genome. Phylogenetic analysis showed that PoTLRs were highly conserved in olive flounder. The analysis of motif prediction and gene structure indicated that TLRs had high sequence similarity. The expression patterns in developmental stages and different tissues showed that TLR members were spatially and temporally specific. RNA-Seq analysis of temperature stress and Edwardsiella tarda infection suggested that TLR members were involved in inflammatory responses, PoTLR5b and PoTLR22 showed significant differences in response to both temperature stress and E. tarda stress, indicating their potential immune functions. The results of this study suggested that TLR genes played important roles in the innate immune response of olive flounder, and would provide a solid basis for further study of their functions.
Assuntos
Infecções por Enterobacteriaceae , Doenças dos Peixes , Linguado , Animais , Linguado/genética , Edwardsiella tarda/fisiologia , Temperatura , Filogenia , Receptores Toll-Like/genética , Imunidade Inata/genética , Infecções por Enterobacteriaceae/veterináriaRESUMO
Dual-specificity Phosphatases (DUSPs) are not only the key regulators of dephosphorylating and inactivating mitogen-activated protein kinases (MAPKs), but play a crucial role in the immune response. However, the role of DUSP genes in Japanese flounder (PoDUSPs) is still unclear. In this study, 28 DUSP genes in Japanese flounder were identified and classified based on the whole genome database. Phylogenetic analysis and protein structure analysis revealed that DUSPs had highly conserved domains in teleosts. Molecular evolution analysis indicated that the PoDUSP genes were conservative during evolution and were functional-constrained. Meanwhile, PoDUSP genes were found to express in different embryonic and larval stages which might play the role of sentinel in healthy organisms. Furthermore, PoDUSP genes' expression profiles after temperature stress and Edwardsiella tarda (E. tarda) infection were determined in Japanese flounder without precedent, and the results demonstrated that Podusp1, Podusp2 and Podusp16 were more respective to temperature variation whereas Podusp1 and Podusp6 were more respective to E. tarda infection. In summary, our results provide useful resources for understanding the immune responsibilities of DUSP genes in flatfish.
Assuntos
Infecções por Enterobacteriaceae , Doenças dos Peixes , Linguado , Animais , Fosfatases de Especificidade Dupla/química , Edwardsiella tarda/fisiologia , Proteínas Quinases Ativadas por Mitógeno/genética , Filogenia , TemperaturaRESUMO
The complement system plays an important role in immune regulation and acts as the first line of defense against any pathogenic attack. To comprehend the red sea bream (Pagrus major) immune response, three complement genes, namely, pmC1r, pmMASP and pmC3, belonging to the classical, lectin and alternative complement cascade, respectively, were identified and characterized. pmC1r, pmMASP, and pmC3 were comprised of 2535, 3352, and 5735 base mRNA which encodes 732, 1029 and 1677 aa putative proteins, respectively. Phylogenetically, all the three studied genes clustered with their corresponding homologous clade. Tissue distribution and cellular localization data demonstrated a very high prevalence of all the three genes in the liver. Both bacterial and viral infection resulted in significant transcriptional alterations in all three genes in the liver with respect to their vehicle control counterparts. Specifically, bacterial challenge affected the pmMASP and pmC3 expression, while the viral infection resulted in pmC1r and pmC3 mRNA activation. Altogether, our data demonstrate the ability of pmC1r, pmMASP and pmC3 in bringing about an immune response against any pathogenic encroachment, and thus activating, not only one, but all the three complement pathways, in red sea bream.
Assuntos
Proteínas do Sistema Complemento/genética , Proteínas do Sistema Complemento/imunologia , Doenças dos Peixes/imunologia , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Dourada/genética , Dourada/imunologia , Animais , Infecções por Vírus de DNA/imunologia , Edwardsiella tarda/fisiologia , Infecções por Enterobacteriaceae/imunologia , Proteínas de Peixes/genética , Proteínas de Peixes/imunologia , Perfilação da Expressão Gênica/veterinária , Iridoviridae/fisiologia , FilogeniaRESUMO
Our previous studies demonstrated that molecular breeding via DNA shuffling directs the evolution of polyvalent vaccines with desired traits, which leads to generation of polyvalent ompA vaccines using Vibrio alginolyticus VA0764 primers. Here, we replaced VA0764 primers with Edwardsiella tarda ompA primers to generate new polyvalent ompA vaccines by DNA shuffling of the same five ompA genes from four species of bacteria E. tarda, V. parahaemolyticus, V. alginolyticus and Escherichia coli. We identified four polyvalent vaccine candidates from a eukaryotic expressing library EompAs-FE containing 82 ompAs using active immune protection against V. alginolyticus and E. tarda. Furthermore, we explored mechanisms of polyvalent vaccine candidates by investigation of the innate immune response to these ompAs, and found that expression of IL-1ß, IL-8, IL-15, COX-2, IFN-γ, TLR-1, TLR-3 and C3b genes was elevated as a characteristic feature of these polyvalent vaccine candidates. These results indicate that use of different primers to construct a DNA library selects new evolution of polyvalent vaccines with desired traits, and polyvalent ompA vaccines elicit high innate immune response.
Assuntos
Proteínas da Membrana Bacteriana Externa/imunologia , Vacinas Bacterianas/imunologia , Edwardsiella tarda/imunologia , Doenças dos Peixes/imunologia , Vibrio alginolyticus/imunologia , Peixe-Zebra , Animais , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/imunologia , Embaralhamento de DNA/veterinária , Edwardsiella tarda/genética , Infecções por Enterobacteriaceae/imunologia , Infecções por Enterobacteriaceae/microbiologia , Infecções por Enterobacteriaceae/veterinária , Escherichia coli/genética , Escherichia coli/imunologia , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/imunologia , Doenças dos Peixes/microbiologia , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Vibrioses/imunologia , Vibrioses/microbiologia , Vibrioses/veterinária , Vibrio alginolyticus/genética , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/imunologiaRESUMO
A total of 127 wild eels caught in the L'Albufera Lake (Spain) and 24 samples of lagoon freshwater were analysed for 1-year period. Edwardsiella strains were isolated from liver/kidney on TSA-1 plates in 31.9% of total diseased specimens, and the edwardsiellosis prevalence in the fishery was of 11.8%. The use of double-strength Salmonella-Shigella (DSSS) broth and SS agar yielded Edwardsiella isolation from intestine in 100% of those edwardsiellosis-diseased eels, but also in 40.4% of other sick fish with vibriosis or aeromonosis and in 28.8% of healthy eels, as well as from freshwater in 8.3% of samples. Pure cultures were isolated on SS agar from the former, but motile Aeromonas, Plesiomonas shigelloides and Hafnia alvei were recovered along with Edwardsiella in the other samples. Edwardsiella isolates identification at species level revealed that E. piscicida was distributed between wild eels and freshwater but E. tarda only did in freshwater. All E. piscicida strains were virulent for eels (LD < 1.0 × 106 CFU/fish) but that of E. tarda was not. This is the first report of E. piscicida in wild eel intestines and natural freshwater, highlighting its role as potential reservoirs for the bacterium. A seasonal recovery was found for E. piscicida at water temperature above 20°C.
Assuntos
Anguilla , Edwardsiella/isolamento & purificação , Infecções por Enterobacteriaceae/veterinária , Doenças dos Peixes/epidemiologia , Lagos/microbiologia , Animais , Edwardsiella/patogenicidade , Infecções por Enterobacteriaceae/epidemiologia , Infecções por Enterobacteriaceae/microbiologia , Doenças dos Peixes/microbiologia , Incidência , Prevalência , Espanha/epidemiologia , VirulênciaRESUMO
TLR5 is one of the important PRR (pathogen recognition receptors) and plays a fundamental role in pathogen recognition and activation of innate immune responses. It recognizes bacterial flagellin and stimulates the production of proinflammatory cytokines, through signalling via the adaptor protein MyD88. In this study, we characterized partial TLR5 (soluble form) gene from Pangasianodon hypophthalmus and analysed its expression profile upon challenge by Edwardsiella tarda. Bioinformatic analysis of gene sequence revealed a putative protein of 266 amino acids with four Leucine rich repeats. Quantitative expression analysis of TLR 5S showed its wide distribution in various organs and tissues. However, significant expression of TLR5S was observed in liver and spleen at 12 h (â¼207.8 fold, p < 0.05). Significant upregulation was observed in kidney at 72 h.p.i. (50 folds, p < 0.05) indicating that the kidney provides longer protection almost till the activation of the adaptive immune system. This study enriches the knowledge of TLR5S in boosting the innate immunity against bacterial invasion in fish.
Assuntos
Peixes-Gato/imunologia , Infecções por Enterobacteriaceae/veterinária , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Regulação da Expressão Gênica , Imunidade Inata , Receptor 5 Toll-Like/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Peixes-Gato/classificação , Peixes-Gato/genética , DNA Complementar/genética , DNA Complementar/metabolismo , Edwardsiella tarda/imunologia , Infecções por Enterobacteriaceae/genética , Infecções por Enterobacteriaceae/imunologia , Proteínas de Peixes/química , Proteínas de Peixes/imunologia , Perfilação da Expressão Gênica , Especificidade de Órgãos , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Alinhamento de Sequência/veterinária , Receptor 5 Toll-Like/química , Receptor 5 Toll-Like/imunologiaRESUMO
The artificially synthesized polyinosinic-polycytidylic acid (poly IC) has been widely used to induce type I IFN responses in various vertebrates including fish. However, as poly IC is too expensive to use in aquaculture, the development of another economical long dsRNA producing method is needed to practically use long dsRNAs in aquaculture farms for the control of infectious diseases. In the present study, to produce long dsRNAs economically, we developed a novel long dsRNA production system based on the RNase III gene deleted auxotrophic mutant E. tarda (ΔalrΔrncΔasd E. tarda) and a long dsRNA-producing vector that was equipped with two modified λ phage PR promoters arranged in a head-to-head fashion. As the present genetically engineered E. tarda cannot live without supplementation of d-alanine and DAP, environmental and medicinal risks are minimized. Olive flounder (Paralichthys olivaceus) fingerlings administered the long dsRNA-producing auxotrophic E. tarda mutant (Δalr ΔrncΔasd E. tarda) showed significantly higher expressions of TLR22, Mx1, and ISG15 genes, indicating a potential to increase type I interferon responses.
Assuntos
Aquicultura/métodos , Proteínas de Bactérias/metabolismo , Edwardsiella tarda/genética , Linguados/imunologia , RNA Bacteriano/metabolismo , RNA de Cadeia Dupla/administração & dosagem , Ribonuclease III/genética , Adjuvantes Imunológicos/administração & dosagem , Adjuvantes Imunológicos/metabolismo , Animais , Técnicas de Inativação de Genes/veterinária , Imunização/veterinária , RNA de Cadeia Dupla/genética , RNA de Cadeia Dupla/metabolismoRESUMO
Molecular breeding via DNA shuffling directs the evolution of vaccines with desired traits. In the present study, polyvalent OmpA vaccines were generated by DNA shuffling of five ompA genes from four species of bacteria Vibrio parahaemolyticus, V. alginolyticus, Edwardsiella tarda and Escherichia coli. First, a new hybrid OmpA was constructed using VA0764 primers and used for construction of a prokaryotic expressing library PompAs-FV containing 84 ompAs, which were validated by PCR and SDS/PAGE. Then, the 84 ompAs were used to construct a eukaryotic expressing library EompAs-FV for preparing DNA vaccines. Third, extracellular bacterium V. alginolyticus challenge post active immunization using these DNA vaccines was carried out to identify genes with high immunoprotection. Among the 84 ompAs, 17 showed higher or equal immune protection against infection caused by V. alginolyticus than control VA0764. Finally, immune protection against infection caused by intracellular bacterium Edwardsiella tarda was assessed further using the top seven out of the 17 ompAs. This led to identification of three efficient polyvalent vaccines against infections caused by the extracellular bacterium V. alginolyticus and intracellular bacterium E. tarda. In addition, we sequenced genes for understanding mechanisms of the polyvalent vaccines, but association of immune protection with mutation of gene and amino acids is not determined. These results indicate that DNA shuffling is an efficient way to develop polyvalent vaccines against microbial infections.
Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Vacinas Bacterianas/imunologia , Edwardsiella tarda/imunologia , Escherichia coli/imunologia , Doenças dos Peixes/prevenção & controle , Vibrio/imunologia , Peixe-Zebra , Animais , Proteínas da Membrana Bacteriana Externa/metabolismo , Embaralhamento de DNA/veterinária , Infecções por Enterobacteriaceae/imunologia , Infecções por Enterobacteriaceae/microbiologia , Infecções por Enterobacteriaceae/prevenção & controle , Infecções por Enterobacteriaceae/veterinária , Infecções por Escherichia coli/imunologia , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/prevenção & controle , Infecções por Escherichia coli/veterinária , Vacinas contra Escherichia coli/imunologia , Doenças dos Peixes/imunologia , Doenças dos Peixes/microbiologia , Vibrioses/imunologia , Vibrioses/microbiologia , Vibrioses/prevenção & controle , Vibrioses/veterinária , Vibrio alginolyticus/imunologia , Vibrio parahaemolyticus/imunologiaRESUMO
Edwardsiella tarda, the causative agent of Edwardsiellosis, imposes medical challenges in both the clinic and aquaculture. The emergence of multidrug resistant strains makes antibiotic treatment impractical. The identification of molecules that facilitate or promote antibiotic efficacy is in high demand. In the present study, we aimed to identify small molecules whose abundance is correlated with kanamycin resistance in E. tarda by gas chromatography-mass spectrometry. We found that the abundance of fructose was greatly suppressed in kanamycin-resistant strains. The incubation of kanamycin-resistant bacteria with exogenous fructose sensitized the bacteria to kanamycin. Moreover, the fructose also functioned in bacteria persisters and biofilm. The synergistic effects of fructose and kanamycin were validated in a mouse model. Furthermore, the mechanism relies on fructose in activating TCA cycle to produce NADH, which generates proton motive force to increase the uptake of the antibiotics. Therefore, we present a novel approach in fighting against multidrug resistant bacteria through exploration of antibiotic-suppressed molecules.
Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Edwardsiella tarda/efeitos dos fármacos , Frutose/farmacologia , Canamicina/farmacologia , Testes de Sensibilidade MicrobianaRESUMO
A technology for inactivation of pathogens in human blood products by treatment with amotosalen hydrochloride (S-59) in combination with long wavelength ultraviolet light (UVA) to decrease transfusion-mediated sepsis has been applied to make safe vaccines against human pathogenic bacteria, and the resultants were called killed but metabolically active (KBMA) bacteria. In the present study, we first generated KBMA Edwardsiella tarda and evaluated its potential as a protective vaccine in olive flounder (Paralichthys olivaceus). To prevent the restoration of division ability by removal of psoralen adducts in the bacterial chromosome through the nucleotide excision repair (NER), the uvrA and uvrB genes knock-out E. tarda (ΔuvrAB E. tarda) was produced by the allelic exchange method. The optimal condition for generation of KBMA E. tarda was exposure of the ΔuvrAB E. tarda to 100 ng/ml of S-59 and 2.8 J/cm(2) of UVA irradiation. The KBMA E. tarda could not replicate but showed a high metabolic activity (measured by lactate dehydrogenase activity) that was comparable to the wild-type E. tarda. In comparison of survival rates between groups vaccinated with the same dose of bacteria, fish immunized with KBMA E. tarda showed significantly higher survival rates than fish immunized with formalin-killed cell (FKC) E. tarda. Furthermore, fish immunized with 1 × 10(7) CFU/fish of KBMA E. tarda showed no mortality, while PBS-injected fish showed 100% mortality. The serum agglutination titer was sharply increased by 10(7) CFU/fish of KBMA E. tarda compared to those of fish immunized with 10(6) CFU/fish of KBMA E. tarda or 10(7) CFU/fish of FKC E. tarda. The consistently lower serum agglutination titers against KBMA E. tarda than against FKC E. tarda in both KBMA and FKC E. tarda immunized groups suggest that some factors secreted from KBMA E. tarda might inhibit the serum agglutination activity. In conclusion, the present results showed the higher potential of KBMA E. tarda than FKC E. tarda as a prophylactic vaccine.
Assuntos
Vacinas Bacterianas/imunologia , Edwardsiella tarda/imunologia , Infecções por Enterobacteriaceae/veterinária , Doenças dos Peixes/prevenção & controle , Linguados , Vacinação/veterinária , Animais , Infecções por Enterobacteriaceae/imunologia , Infecções por Enterobacteriaceae/prevenção & controle , Doenças dos Peixes/imunologia , Doenças dos Peixes/microbiologia , Vacinas de Produtos Inativados/imunologiaRESUMO
Previously generated two auxotrophic genes knockout Edwardsiella tarda (Δalr Δasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Δalr Δasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by Miamiensis avidus in olive flounder (Paralichthys olivaceus) was investigated. The auxotrophic E. tarda mutant with pL-CpG30 plasmids elicited evidently higher survival rates and delayed both onset of mortality and time-to-death of olive flounder against M. avidus challenge. Furthermore, administration of E. tarda mutant that contains plasmids enriched in CpG 1668 motif elicited clearly higher survival rates of fish that were already infected with M. avidus. These results indicate that CpG 1668 plasmids-harboring E. tarda mutant may provide not only preventive measure but also therapeutic approach against scuticociliatosis in olive flounder.
Assuntos
Infecções por Cilióforos/veterinária , Ilhas de CpG/genética , Edwardsiella tarda/genética , Doenças dos Peixes/terapia , Linguado/parasitologia , Oligoimenóforos , Animais , Infecções por Cilióforos/prevenção & controle , Infecções por Cilióforos/terapia , Doenças dos Peixes/prevenção & controle , Pesqueiros , Técnicas de Inativação de Genes , Mutação , Motivos de Nucleotídeos/genética , Plasmídeos , Distribuição AleatóriaRESUMO
The genus Edwardsiella, previously residing in the family Enterobacteriaceae and now a member of the family Hafniaceae, is currently composed of five species, although the taxonomy of this genus is still unsettled. The genus can primarily be divided into two pathogenic groups: E. tarda strains are responsible for almost all human infections, and two other species (E. ictaluri, E. piscicida) cause diseases in fish. Human infections predominate in subtropical habitats of the world and in specific geospatial regions with gastrointestinal disease, bloodborne infections, and wound infections, the most common clinical presentations in decreasing order. Gastroenteritis can present in many different forms and mimic other intestinal disturbances. Chronic gastroenteritis is not uncommon. Septicemia is primarily found in persons with comorbid conditions including malignancies and liver disease. Mortality rates range from 9% to 28%. Most human infections are linked to one of several risk factors associated with freshwater or marine environments such as seafood consumption. In contrast, edwardsiellosis in fish is caused by two other species, in particular E. ictaluri. Both E. ictaluri and E. piscicida can cause massive outbreaks of disease in aquaculture systems worldwide, including enteric septicemia in channel catfish and tilapia. Collectively, these species are increasingly being recognized as important pathogens in clinical and veterinary medicine. This article highlights and provides a current perspective on the taxonomy, microbiology, epidemiology, and pathogenicity of this increasingly important group.
RESUMO
Edwardsiella piscicida is an important fish pathogen responsible for economic losses in global aquaculture, and E. tarda is also a human zoonotic pathogen. In this study, the survival of E. piscicida and E. tarda strains kept in filtered and sterilized lake water microcosms was investigated during a 20-week period at 7 °C, 15 °C and 25 °C, as well as its pathogenicity retention during a starvation period. E. tarda V43.2 stayed culturable for 6 weeks at 7 °C, 9 weeks at 25 °C and 12 weeks at 15 °C. Both E. piscicida strains (V12.1 and V57.2) stayed culturable even longer, for at least 12 weeks at 7 °C, 15 °C and 25 °C under the same starvation conditions. After Edwardsiella cells entered into the VBNC state, some became shorter and "rounded up," but others aggregated and retained a short rod shape. Aggregates of Edwardsiella cells were common throughout the VBNC period, and a well-formed biofilm was observed for all tested strains at the end of the experiment. The growth capacity of VBNC cells was restored by cultivating microcosm water samples in LB broth at 28 °C. Resuscitated E. piscicida cells were as virulent for the European eel as the controls. Natural waters can be a reservoir for Edwardsiella, and its underestimation in environmental samples poses a risk to public health and aquaculture.
RESUMO
The overuse of antibiotics to control bacterial pathogens leads to the generation of their antibiotic-resistant strains including Edwardsiella tarda. Understanding of mechanisms of the antibiotic resistance is crucial to develop novel methods to manage the infection. Here, two-dimensional electrophoresis-based proteomics was used to characterize balofloxacin-responsive proteins. The altered proteome consisted of 19 proteins with differential abundance, where six metabolic pathways were enriched. The metabolic modulation activated the central carbon metabolism with elevation of NADH, PMF, and ATP. Among the 19 proteins, ETAE_1987 (pre-peptidase) and ETAE_2174 (integration host factor beta subunit) were bound with balofloxacin directly. This was further confirmed by the binding of balofloxacin with recombinant ETAE_1987 and ETAE_2174 using Oxford cup method. Compared with bovine serum albumin, a known balofloxacin-binding protein, ETAE_1987 and ETAE_2174 increased the binding capability by 3.3- and 22-fold, respectively. The combination was validated by microscale thermophoresis. These data characterize the balofloxacin-stressed proteome as a result of the increased central carbon metabolism and energy metabolism and determine ETAE_1987 and ETAE_2174 as balofloxacin-binding proteins. These findings have significant implications in understanding bacterial antibiotic-resistant and drug action mechanisms based on balofloxacin-binding proteins. SIGNIFICANCE: Antibiotic-resistant Edwardsiella tarda strains are frequently isolated and cause a great loss in aquaculture since these bacterial strains are insensitivity to antibiotics. The present study showed that the increased central carbon metabolism forms a characteristic feature of the balofloxacin-stressed proteomics. Furthermore, two proteins, ETAE_1987 (pre-peptidase) and ETAE_2174, of the balofloxacin-stressed proteome were identified as balofloxacin-binding proteins. The binding capability is 0.39⯱â¯0.017 and 2.67⯱â¯0.066â¯ng/µg proteins for ETAE_1987 and ETAE_2174, respectively. These results reveal the elevated central carbon metabolism as a key feature of the balofloxacin-stressed proteomics and pre-peptidase and integration host factor as balofloxacin-binding proteins in E. tarda. These findings are useful in the understanding of bacterial balofloxacin-stressed mechanisms and providing new targets for controlling antibiotic-resistant bacteria.
Assuntos
Proteínas de Transporte/metabolismo , Edwardsiella tarda , Fluoroquinolonas/farmacologia , Fatores Hospedeiros de Integração/metabolismo , Peptídeo Hidrolases/metabolismo , Proteoma/efeitos dos fármacos , Proteínas de Transporte/análise , Proteínas de Transporte/genética , Farmacorresistência Bacteriana/genética , Edwardsiella tarda/efeitos dos fármacos , Edwardsiella tarda/genética , Edwardsiella tarda/metabolismo , Fluoroquinolonas/metabolismo , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Fatores Hospedeiros de Integração/genética , Peptídeo Hidrolases/análise , Peptídeo Hidrolases/genética , Proteoma/genética , Proteoma/metabolismo , Proteômica , Estresse Fisiológico/efeitos dos fármacos , Estresse Fisiológico/genéticaRESUMO
Estrogenic endocrine disrupting chemicals (EEDCs) are present ubiquitously in sediments and aquatic ecosystems worldwide. The detrimental impact of EEDCs on the reproduction of wildlife is widely recognized. Increasing evidence shows the immunosuppressive effects of EEDCs in vertebrates. Yet, no studies have considered concomitantly EEDC-induced impacts on reproductive impairment and immune suppression in vivo, which are deemed essential for risk assessment and environmental monitoring. In this study, EE2 was used as a representative EEDC, for parallel evaluation of EEDC-induced immune suppression (immune marker gene expression, leukocyte numbers, host resistance assay, and immune competence index) and reproductive impairment (estrogen responsive gene expression, fecundity, fertilization success, hatching success, and reproductive competence index) in an established fish model (marine medaka Oryzias melastigma), considering sex-specific induction and adaptation and recovery responses under different EE2 exposure scenarios. The findings in marine medaka reveal distinct sex differences in the EE2-mediated biological responses. For female fish, low concentration of exogenous EE2 (33 ng/L) could induce hormesis (immune enhancement), enable adaptation (restored reproduction) and even boost fish resistance to bacterial challenge after abatement of EE2. However, a prolonged exposure to high levels of EE2 (113 ng/L) not only impaired F0 immune function, but also perturbed females recovering from reproductive impairment, resulting in a persistent impact on the F1 generation output. Thus, for female fish, the exposure concentration of EE2 is more critical than the dose of EE2 in determining the impacts of EE2 on immune function and reproduction. Conversely, male fish are far more sensitive than females to the presence of low levels of exogenous EE2 in water and the EE2-mediated biological impacts are clearly dose-dependent. It is also evident in male fish that direct contact of EE2 is essential to sustain impairments of immune competence and reproductive output as well as deregulation of immune function genes in vivo. The immunomodulatory pathways altered by EE2 were deciphered for male and female fish, separately. Downregulation of hepatic tlr3 and c3 (in female) and tlr3, tlr5 and c3 (in male) may be indicative of impaired fish immune competence. Taken together, impaired immune competence in the EE2-exposed fish poses an immediate thread on the survival of F0 population. Impaired reproduction in the EE2-exposed fish can directly affect F1 output. Parallel evaluation of immune competence and reproduction are important considerations when assessing the risk of sublethal levels of EE2/EEDCs in aquatic environments.
Assuntos
Etinilestradiol/toxicidade , Fatores Imunológicos/toxicidade , Oryzias/fisiologia , Reprodução/efeitos dos fármacos , Caracteres Sexuais , Animais , Biomarcadores/metabolismo , Monitoramento Ambiental , Feminino , Fertilidade/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Leucócitos/efeitos dos fármacos , Leucócitos/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Oryzias/imunologia , Poluentes Químicos da Água/toxicidadeRESUMO
Dietary regime modifications have been an integral part of health and healing practices throughout the animal kingdom. Thus, to assess the effects of periodic starvation and refeeding schedule on the physiological and immunological perturbations in Edwardsiella tarda infected red sea bream, we conducted a 20day experiment using 4 treatment groups, namely, pre-fed placebo (PFP); pre-starved placebo (PSP); pre-fed infected (PFI); and pre-starved infected (PSI), wherein a 5h E. tarda infection was done on the 11th day. In the present investigation, the pre-starved groups showed significant (P<0.05) alterations in the liver Hexokinase and Glucose-6-phosphatase activity. The pre-starved fish also exhibited significant (P<0.05) increment in the hepatosomatic index, along with increased hepatic glycogen content, in a time dependent fashion. The PPAR (peroxisome proliferator activated receptors)α transcription in the pre-starved group decreased significantly (P<0.05) by 10dai, while the PPARγ showcased a reverse pattern. The transcription of Hepcidin1 and Transferrin (iron homeostasis related genes), and Cathepsin D and Ubiquitin (programmed cell death related genes) portrayed a time responsive decrease and increase in PSI and PFI groups, respectively. Additionally, in comparison to the PFI group, the PSI fish demonstrated substantially reduced oxidative stress level. Fluorescent Immunohistochemistry showed significant (P<0.05) increase in p63 positive cells in the 10dai PFI fish in relation to the PSI group. Therefore, these findings provide new insight into the beneficial role of alternating starvation and refeeding schedule, preferably short-term starvation prior to an infection, in order to obtain better capability to battle against E. tarda infection in red sea bream.