The Source of Life: Meditation and Spirituality in Healthcare for a Comprehensive Approach to The COVID-19 Syndemic.

The COVID-19 syndemic has raised many unanswered questions about the most important values in human life. It has revealed  the limits of looking at mere survival and ignoring closeness, spirituality, and "connectedness". Spiritual accompaniment, in contrast, is a valid therapeutic tool for individuals suffering from  life-threatening diseases, allowing a real recovery of the transcendent dimension of existence which retrieves one's relationship with the mystery, and reintegrates illness and death within one's horizon of thought. According to this vision, in the field of healthcare, people experienced in spiritual accompaniment may support patients through their disease journey by strengthening  their resilience; this was extended in 2020 with telematic assistance, to patients with COVID-19, with very positive results. This gave impetus to the project to rebuild a rural village, suitable for pursuing the principles of green therapy (also known as echotherapy) in order to host patients in various stages of life-threatening illness  who wish to deepen their spiritual search by receiving expert, non-confessional spiritual accompaniment, by living side by side with families and resident monks; there will also be a hospice oriented towards spiritual assistance, to accommodate patients in advanced stages of illness. The spiritual accompaniment proposed here is centered on meditation and is part of a historic tradition, although it is promoted with language adapted to the modern era. This has for decades helped many people following this path.

0 °C is better?- Thawing temperature optimization study for cancer cryoablation in a mouse model with green fluorescent protein-labeled Lewis lung cancer.

PURPOSE: There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD: Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control group, T0 group (thawing temperature 0 °C), and T40 group (thawing temperature 40 °C). Cryoablation was performed using a combined surgical system. When the ice ball reached the border of the tumor, they were rewarmed to 0 °C and 40 °C, respectively, using a single freeze-thaw cycle. After the surgery, weight of these mice, length/width and the fluorescence intensity (FI) of the tumors were recorded. All mice were sacrificed on Day 14 after the procedures and their xenografts were excised and weighed immediately. We also checked for pulmonary metastasis, and examined tumor specimens using HE staining. RESULTS: Body weights, tumor volumes and FI in the three groups did not differ significantly at baseline. On Day 14, 39% of the tumors in the T0 group decreased in volume, whereas only 17% in the T40 group did. The average FI in the control group increased by 60%, but declined by 72% in T0 mice and 69% in T40 mice. Tumor inhibition rates were 71.64% in the T0 group and 68.12% in the T40 group. Lung metastases rates and histological changes were compatible between the two intervention groups. CONCLUSION: Using 0 °C as the thawing temperature may have more potential benefits in cryoablation efficacy.

Non-extended cryoablation could be a new strategy in lung cancer management: An experiment on green fluorescent protein-labeled Lewis lung cancer-bearing mice.

Modern cryoablation has been performed in solid tumor management for more than two decades. Following the surgical spirits, it seems natural to pursue radical procedures in clinical practice, which results in unnecessary adverse effects. The attempt to use non-extended procedure made some marked achievements in practice but was criticized severely, because it was supposed to induce residual tumors, which would trigger the rapid development of cancer. Oncologists favored this procedure, however, claiming that non-extended cryoablation let lung cancer patients have higher quality of lives and longer survivals, in light of clinical observations. Therefore, this study was conducted trying to solve this controversy. In this study, fifty female C57BL/6J mice were grafted green fluorescent protein (GFP)-labeled Lewis lung cancer and randomized into two groups. The bidirectional diameters and fluorescence intensity of tumors, and the body weight of mice were recorded. Two weeks after the intervention, tumor volumes increased 20.95% in the cryoablation group, significantly different from that in the control group; the fluorescence intensity decreased 49.85% in the cryoablation group but increased 125.07% in the control group. Lung metastases could be observed in only 20% of mice in the cryosurgery group, contrasted to 64% in the control group. The non-extended lung cancer cryoablation does induce marginal tumor residuals, but will not trigger rapid tumor development. Inversely, the residual tumor cells are severely struck and the metastases are suppressed after the intervention. It could be a new strategy in lung cancer management, even for patients not in early stage.

Synergistic Antibacterial Potential of 6-Pentyl-α-pyrone Lactone and Zinc Oxide Nanoparticles against Multidrug-Resistant Enterobacterales Isolated from Urinary Tract Infections in Humans.

Urinary tract infection (UTI) is one of the most common bacterial infections in the world, which is associated with high morbidity and mortality rates. Enterobacterales species are considered the most causative agent for UTI, especially uropathogenic Escherichia coli (UPEC). Here, we investigated the antibacterial activity of the green fungal metabolite, 6-pentyl α pyrone lactone, alone or in combination with zinc oxide nanoparticles (ZnONPs) against multidrug-resistant Enterobacterales recovered from UTI. The results revealed that 57.27% of human urine samples were positive for Enterobacterales, where E. coli was the most prevalent bacterial pathogen (66.67%). Of note, 98.41% of Enterobacterales isolates were multidrug-resistant (MDR) with multiple antimicrobial resistance (MAR) indices ranged from 0.437 to 1. Fifty percent of the examined isolates were positive for the integrase gene; 60% out of them harbored class 2 integron, whereas the other 40% carried class 1 integrons. The broth microdilution assay ensured that the 6-pentyl-α-pyrone lactone had a reasonable antimicrobial effect against the examined isolates (Minimum inhibitory concentration (MIC) values of 16-32 µg/mL). However, ZnONPs showed a strong antimicrobial effect against the investigated isolates with MIC values ranging from 0.015 to 32 µg/mL. Interestingly, the MICs decreased 5-12 fold and 3-11 fold for 6-pentyl-α-pyrone lactone and ZnONPs, respectively, against examined isolates after their combination. This is the first report suggesting the use of 6-pentyl α pyrone lactone and ZnONPs combination as a promising candidate against MDR Enterobacterales recovered from UTI.

Horticultural Therapy May Reduce Psychological and Physiological Stress in Adolescents with Anorexia Nervosa: A Pilot Study.

Studies in psychiatric populations have found a positive effect of Horticultural therapy (HCT) on reductions in stress levels. The main objective of the present pilot study was to evaluate the impact of the addition of HCT to conventional clinical treatment (Treatment as Usual, TaU) in a sample of six female adolescents with anorexia nervosa restricting type (AN-R), as compared to six AN-R patients, matched for sex and age, under TaU only. This is a prospective, non-profit, pilot study on patients with a previous diagnosis of AN-R and BMI < 16, recruited in 2020 in clinical settings. At enrolment (T0) and after treatment completion (TF), psychiatric assessment was performed. At T0, all the patients underwent: baseline electrocardiogram acquisition with a wearable chest strap for recording heart rate and its variability; skin conductance registration and thermal mapping of the individual's face. An olfactory identification test was administered both to evaluate the olfactory sensoriality and to assess the induced stress. One-way analyses of variance (ANOVAs) were performed to analyze modifications in clinical and physiological variables, considering time (T0, TF) as a within-subjects factor and group (experimental vs. control) as between-subjects factors. When the ANOVA was significant, post hoc analysis was performed by Paired Sample T-tests. Only in the HCT group, stress response levels, as measured by the biological parameters, improved over time. The body uneasiness level and the affective problem measures displayed a significant improvement in the HCT subjects. HCT seems to have a positive influence on stress levels in AN-R.

Redefinition of Park Design Criteria as a Result of Analysis of Well-Being and Soundscape: The Case Study of the Kortowo Park (Poland).

Well-being and soundscape analysis should be useful in re-design works involved in the planning of recreational areas and green spaces on campuses to improve the health of students, academics, and university staff. Proper space planning, for example, in campus parks, including the planning of soundscape zones, is important in relieving study and work stress. The aim of the study was to conduct a multicriteria analysis of the soundscape and well-being of users in the university park on campus in Olsztyn (Poland). It was important to redefine thinking about the re-composition of park space, including reduction of noise and improving well-being. The research included: 1. measurements of sound pressure levels (SPL) at selected points in two periods, 2. interview with park users and preparation of a mental map, 3. experts' opinion on soundscape and well-being, and 4. design schemes for re-design of the park. The results of research regarding the perception of the soundscape and well-being in Kortowo park by respondents differ slightly from the results of SPL measurements. The results also confirm the difference between SPL in the leafless and leafy period. The results show a clear relationship between the perception of sounds and well-being in the park. However, in some areas near the water, where higher noise levels are reported the respondents felt quite comfortable. Finally, design schemes are proposed, based on experts' opinions and results of the analysis.