Synergistic effects of chemical additives and mature compost on reducing H2S emission during kitchen waste composting.

Additives could improve composting performance and reduce gaseous emission, but few studies have explored the synergistic of additives on H2S emission and compost maturity. This research aims to make an investigation about the effects of chemical additives and mature compost on H2S emission and compost maturity of kitchen waste composting. The results showed that additives increased the germination index value and H2S emission reduction over 15 days and the treatment with both chemical additives and mature compost achieved highest germination index value and H2S emission reduction (85%). Except for the treatment with only chemical additives, the total sulfur content increased during the kitchen waste composting. The proportion of effective sulfur was higher with the addition of chemical additives, compared with other groups. The relative abundance of H2S-formation bacterial (Desulfovibrio) was reduced and the relative abundance of bacterial (Pseudomonas and Paracoccus), which could convert sulfur-containing substances and H2S to sulfate was improved with additives. In the composting process with both chemical additives and mature compost, the relative abundance of Desulfovibrio was lowest, while the relative abundance of Pseudomonas and Paracoccus was highest. Taken together, the chemical additives and mature compost achieved H2S emission reduction by regulating the dynamics of microbial community.

Effects of compost-derived humic acid on the bio-dechlorination of pentachlorophenol in high iron content paddy soil.

Pentachlorophenol (PCP) is a common residual organic pollutant in paddy soil, and its harmful effects on soil ecosystems have been confirmed. Humic acid (HA) could act as an electron shuttle to promote the reductive dechlorination of PCP under anaerobic conditions. Humic-like substances produced by composting of kitchen waste were able to facilitate the reductive dechlorination of PCP during Fe(III) oxide reduction by iron-reducing bacteria. However, the effects of compost-derived HAs on reductive dechlorination of PCP in a paddy soil system with a high iron content have not been fully confirmed. The characteristics of HAs from different stages of composting during bio-dechlorination of PCP were still unclear. The functional components of compost-derived HAs, which are responsible for reductive dechlorination of PCP in different stages of composting, also need further investigation. In this study, we conducted a series of experiments on the Guangdong paddy soil system with high iron content (17.5 mg kg-1) to investigate the reductive dechlorination of PCP by HA in the early, middle, and later stages of food waste composting. The results showed that the middle- and late-stages of compost-derived HAs all promoted reductive dechlorination of PCP in the paddy system, but it was opposite in the early-stage. Significant differences were also presented in the components of HAs from different stages of composting. The early-stage compost-derived HAs contain numerous easy degradable components, it would inhibit the dechlorination of PCP by the changes of microbial metabolism in paddy soil. Compost-derived HAs in the middle composting stage showed the best reductive dechlorination effects on PCP. The reason might be that the compost-derived HAs in the middle composting stage could act both as electron donors and transfers. The electron transfer capacities (ETC) of middle-stage compost-derived HAs were significantly higher than those in the early and later composting stages. Compared with the natural HAs in the soil system, compost-derived HAs contained more chlorinated products with lower toxicities after the PCP degradation. This result mainly contributed to the detoxification and mineralization of PCP in the soil. These findings clarified the effects of compost-derived HAs on PCP bio-dechlorination in paddy soil with high iron content, identifying the optimal phase of compost-derived HA and providing a theoretical basis for the utilization of kitchen waste composting as a resource of HA.

Potential of novel iron 1,3,5-benzene tricarboxylate loaded on biochar to reduce ammonia and nitrous oxide emissions and its associated biological mechanism during composting.

In this study, a novel iron 1,3,5-benzene tricarboxylate loaded on biochar (BC-FeBTC) was developed and applied to kitchen waste composting. The results demonstrated that the emissions of NH3 and N2O were significantly reduced by 57.2% and 37.8%, respectively, compared with those in control group (CK). Microbiological analysis indicated that BC-FeBTC addition altered the diversity and abundance of community structure as well as key functional genes. The nitrification genes of ammonia-oxidizing bacteria were enhanced, thereby promoting nitrification and reducing the emission of NH3. The typical denitrifying bacterium, Pseudomonas, and critical functional genes (nirS, nirK, and nosZ) were significantly inhibited, contributing to reduced N2O emissions. Network analysis further revealed the important influence of BC-FeBTC in nitrogen transformation driven by functional microbes. These findings offer crucial scientific foundation and guidance for the application of novel materials aimed at mitigating nitrogen loss and environmental pollution during composting.

Demonstrating the Key Role of Bacillus in Poly Lactic Acid Film Degradation through Statistical Analysis and Strain Screening.

Plastic films are extensively utilized in agriculture, construction, and manufacturing, with their annual production reaching staggering figures. Addressing the global plastic pollution crisis is imperative. One promising approach is the augmentation of plastic films degradation through microbial agents. Consequently, we undertook composting experiments employing various plastics, including Polyethylene (PE), Poly lactic acid (PLA), and a treatment without plastic films addition (CK), mixed with kitchen waste. Employing bipartite association networks and difference significance analysis methods, we scrutinized the impact of different plastics on the microbial community within the compost piles. There were significant disparities in the microbial community composition among three composting piles. To pinpoint the key microorganisms responsible for PLA degradation, we conducted a comparative analysis of microbial species present on PLA compost piles and PLA film surfaces (PLAS), utilizing variance analysis, co-occurrence network analysis, and Spearman's correlation analysis. Our findings identified Bacillus as the pivotal microorganism involved in PLA degradation. Furthermore, employing function prediction by PICRUSt 2, we identified K00016 as the crucial gene facilitating PLA degradation by Bacillus. Subsequently, employing strain screening techniques, we isolated a highly effective PLA-degrading bacterium, Bacillus amyloliquefaciens strain ML274. The PLA films degradation rate of ML274 reached 3.18%. and other strains was lower than 3.0%. Thus, Bacillus emerges as the primary microorganism driving PLA degradation, emphasizing the significance of focusing on Bacillus genus microorganisms in the development of plastic-degrading bacterial agents for future endeavors.

Hydrothermal pretreatment and compound microbial agents promoting high-quality kitchen waste compost: Superior humification degree and reduction of odour.

Present study investigated the effects of hydrothermal pretreatment (HTP) and addition of compound microbial agent (CMA) on humification, odour generation and metabolism functions of bacterial communities during composting of kitchen waste (KW). Surprisingly, HTP and CMA addition treatment could promote the humification of compost and the control of odour units in contrast to the control (without HTP and CMA addition). The humic acid to fulvic acid ratio of end compost increase by 187.30 %, while humification index (HIX) increased by 18.87 %. 3D-EEM fluorescence spectroscopy of dissolved organic matter (DOM) demonstrated that it facilitated the synthesis of humified compounds and the decomposition of biodegradable compounds. Moreover, the SUVA254, SUVA280 and E253/E203 increased by 118.6 %, 115.25 % and 42.11 % after HTP and CMA addition indicating an increase in aromatic carbon abundance. VFAs had the higher degradation rate (84.91 %) than other treatments (57.46-77.72 %). Meanwhile, the main contributor to the malodorous odour was isovaleric acid, followed by butyric acid and acetic acid during composting. Mantel test indicated that the humification degree was significantly influenced by environmental parameters (temperature, pH, etc.) and metabolic products (HA, DOC and VFAs). Metagenomic analysis indicated that the biodegradation processes at the thermophilic stage were controlled mainly through genes involved in microbial metabolism. HTP and CMA addition was an eco-friendly and efficient strategy to reduce odour emission and improve the compost quality.

Role in aromatic metabolites biodegradation and adverse implication of denitrifying microbiota in kitchen waste composting.

BACKGROUND: Understanding the functional diversity, composition, and dynamics of microbiome is critical for quality in composting. Denitrifying microbiota, possessing multiple metabolic pathways simultaneously. Denitrification-based biodegradation of aromatic metabolites has been widely applied in the bioremediation of sediments. However, role in biodegradation of denitrifying microbiota in kitchen waste composting remain unclear. In this study, microbiome and metabolome were used to comprehensively decipher the relationship of denitrifying microbiota and aromatic metabolites, and its implication in kitchen waste (KW) composting. RESULTS: This study was investigated by adjusting moisture content 60% as control test (CK), 70% as denitrification test (DE). In addition, one tests referred as DE + C, which received 10% of biochar to amend denitrification. Results indicated the quantities of denitrification genes narG were 1.22 × 108 copies/g in DE at the 55th day, which were significantly higher than that in CK and DE + C (P < 0.05). Similarly, the abundance of nirK gene also significantly increased in DE (P < 0.05). The relative abundance of denitrification-related microbes in DE was higher than that in CK, DE + C could weaken their abundance. Metabolomics results demonstrated that metabolites were downgraded in aromatic amino acid and catechin metabolic pathways in DE, which were identified as precursors to synthesis key product fulvic acid. The concentrations of fulvic acid dramatically decreased 21.05 mg/g in DE comparison with CK. Biochar addition alleviated the biodegradation of aromatic metabolites and reduced the utilization of fulvic acid. Integrative analyses of metabolomics and microbiome suggested that the microbiota involved in nitrite reduction pathway was vital for the biodegradation aromatic metabolites. Mantel test verified that NO3--N, moisture content, eta, environmental factors were important drivers behind the changes in the denitrifying microbiota biodegradation function. CONCLUSION: The data confirm the biodegradation function of denitrifying microbiota led to the loss of core product fulvic acid in KW composting, which highlighted the adverse role and implication of denitrification for composting humification. Control of denitrification with biochar was recommended to improve composting quality.

Bacterial dynamics for gaseous emission and humification during bio-augmented composting of kitchen waste with lime addition for acidity regulation.

This study investigated the impacts of lime addition and further microbial inoculum on gaseous emission and humification during kitchen waste composting. High-throughput sequencing was integrated with Linear Discriminant Analysis Effect Size (LEfSe) and Functional Annotation of Prokaryotic Taxa (FAPROTAX) to decipher bacterial dynamics in response to different additives. Results showed that lime addition enriched bacteria, such as Taibaiella and Sphingobacterium as biomarkers, to strengthen organic biodegradation toward humification. Furthermore, lime addition facilitated the proliferation of thermophilic bacteria (e.g. Bacillus and Symbiobacterium) for aerobic chemoheterotrophy, leading to enhanced organic decomposition to trigger notable gaseous emission. Such emission profile was further exacerbated by microbial inoculum to lime-regulated condition given the rapid enrichment of bacteria (e.g. Caldicoprobacter and Pusillimonas as biomarkers) for fermentation and denitrification. In addition, microbial inoculum slightly hindered humus formation by narrowing the relative abundance of bacteria for humification. Results from this study show that microbial inoculum to feedstock should be carefully regulated to accelerate composting and avoid excessive gaseous emission.

Comparison between cold plasma, ultrasonication, and alkaline hydrogen peroxide pretreatments of garden waste to enhance humification in subsequent composting with kitchen waste: Performance and mechanisms.

This study compared the performance and mechanisms of cold plasma, ultrasonication, and alkali-assisted hydrogen peroxide for garden waste pretreatment to advance humification in composting with kitchen waste. High-throughput sequencing integrated with Functional Annotation of Prokaryotic Taxa was used to relate bacterial dynamics to humification. Results show that all pretreatment techniques accelerated humification by 37.5% - 45.7% during composting in comparison to the control treatment. Ultrasonication and alkalization preferred to decompose lignocellulose to produce humus precursors in garden waste, thereby facilitating humus formation at the beginning of composting. By contrast, cold plasma was much faster and simpler than other pretreatment techniques to effectively disrupt the surface structure and reduce the crystallinity of garden waste to enrich functional bacteria for aerobic chemoheterotrophy, xylanolysis, cellulolysis, and ligninolysis during composting. As such, a more robust bacterial community was developed after cold plasma pretreatment to advance humification at the mature stage of composting.

Bacterial dynamics and functions for gaseous emissions and humification in response to aeration intensities during kitchen waste composting.

This study revealed bacteria dynamics and functions for gaseous emissions and humification during kitchen waste composting under different aeration intensities (i.e. 0.24, 0.36, and 0.48 L kg-1 DM min-1) using high-throughput sequencing with Functional Annotation of Prokaryotic Taxa. Results show that aeration increase restrained bacteria (e.g. Lactobacillus and Acinetobacter) for fermentation, nitrate reduction, and sulphur/sulphate respiration, but enriched thermophilic bacteria (e.g. Thermomonospora and Thermobifida) for aerobic chemohetertrophy, xylanolysis, cellulolysis, and methylotrophy. Thus, high aeration intensity (i.e. above 0.36 L kg-1 DM min-1) effectively alleviated the emission of greenhouse gases and hydrogen sulphide, and meanwhile facilitated the production of humus precursors and ammonia. Nevertheless, humification was limited by the conclusion of composting under high aeration conditions due to the consumption of humus precursors for bacterial activity. Thus, aeration intensity should be regulated at different stages indicated by temperature to balance gaseous emissions and humification during kitchen waste composting.

Regulating bacterial dynamics by lime addition to enhance kitchen waste composting.

This study examined bacterial dynamics in response to lime addition to enhance kitchen waste composting using modular network analysis. Bacterial communities could be separated into three meta-modules corresponding to the mesophilic, thermophilic, and mature stage of composting. Lime addition at 1% (wet weight) suppressed acidogens and denitrifiers (e.g. Lactobacillus and Acinetobacter) at the mesophilic stage to reduce greenhouse gas emissions. The matrix pH and temperature were also increased by lime addition via hydrogen reaction to favor bacterial growth and activity. Thus, thermophilic bacteria (e.g. Thermoactinomycetaceae and Planifilum) were enriched with lime addition to facilitate lignocellulose biodegradation for humus formation at the thermophilic stage. Further lime addition to 1.5% reduced ammonia emission at the thermophilic stage via chemical fixation. Moreover, lime inhibited denitrifiers but proliferated nitrifiers at the mature stage to decrease nitrous oxide emission and enhance nitrate content, respectively. As such, lime addition improved both biotic and abiotic composting performance.

Relating bacterial dynamics and functions to gaseous emissions during composting of kitchen and garden wastes.

This study aims to relate bacterial dynamics to gaseous emissions during the composting of kitchen and garden wastes. High-throughput sequencing and Functional Annotation of Prokaryotic Taxa (FAPROTAX) were used to analyse the bacterial community and potential functions during composting, respectively. Results show that the addition of garden waste up to 15% of the total wet weight of composting materials notably mitigated gaseous emissions and improved maturity during kitchen waste composting. Ammonium nitrogen, temperature, oxygen content, and electrical conductivity were identified as critical factors to impact gaseous emissions. The bacterial community analysis indicated that the proliferation of anaerobes during the storage of kitchen waste induced the dramatic emission of methane (CH4) and nitrous oxide (N2O) at the beginning of composting. Adding garden waste could effectively amend the physiochemical properties of composting materials to reduce the relative abundance of microbes (e.g. Desulfotomaculum and Caldicoprobacter) that contributed to gaseous emissions, but enrich those (e.g. Bacillus and Pseudoxanthomonas) for organic biodegradation. Further analysis by FAPROTAX corroborated that adding garden waste could effectively inhibit relevant microbial metabolisms (e.g. fermentation, nitrite/nitrate respiration and sulphate respiration) and thus alleviate the emission of greenhouse gases and odours during kitchen waste composting.