The Autocatalytic Chemical Reaction of a Soluble Biopolymer Derived from Municipal Biowaste.

The paper discusses the perspectives of further implementation of the autocatalytic properties of a soluble biopolymer (SBP) derived from municipal biowastes for the realisation of a biorefinery producing value-added bio-products for consumer use. The reaction of an SBP and water is reported to cause the depolymerisation and oxidation of the pristine SBP organic matter with the formation of carboxyl-functionalised polymers having lower molecular weight and CO2. These findings demonstrate the oxidation of the SBP via water, which could only occur through the production of O and OH radicals catalysed by the SBP. According to the adopted experimental plan, the anaerobic digestate supplied by an Italian municipal biowaste treatment plant was hydrolysed in pH 13 water at 60 °C. The dry product was re-dissolved in plain water at pH 10 and used as a control against the same solution with hydrogen peroxide at 0.1-3 H2O2 moles per SBP carbon mole added. The control and test solutions were kept at room temperature, in the dark or in a climatic chamber under irradiation with simulated solar light, until the pH of the solutions remained constant. Afterwards, the solutions were processed to recover and analyse the crude soluble products. The present work reports the results obtained for the control solution and for the test solutions treated in the presence and absence of H2O2, with and without pH control, in the dark and under irradiation with simulated solar light.

Mild Chemical Treatment of Unsorted Urban Food Wastes.

Municipal biowastes are conventionally treated by assessed anaerobic and aerobic fermentation to produce biogas, anaerobic digestate, and compost. Low-temperature hydrolysis and the oxidation of the digestate and compost, which are still at the experimental stage, are known to yield water-soluble value-added chemical specialities for use in different sectors of the chemical industry and in agriculture. The present paper reports the application of the two chemical reactions to the biowastes before fermentation. The products obtained in this manner are compared with those obtained from the chemical reactions applied to the fermented biowastes. Based on the experimental results, the paper discusses the expected environmental and economic benefits of the above chemical processes and products in comparison with the products obtained by other known biotechnologies for the valorisation of biomass as a feedstock for the biobased chemical industry. The results point out that a sustainable biowaste-based refinery that produces biofuel and biobased chemicals may be developed by integrating chemical and fermentation technologies.

Methane oxidising bacteria to upcycle effluent streams from anaerobic digestion of municipal biowaste.

Conventional microbial protein production relies on the usage of pure chemicals and gases. Natural gas, which is a fossil resource, is the common input gas for bacterial protein production. Alternative sources for gas feedstock and nutrients can sufficiently decrease the operational cost and environmental impact of microbial protein production processes. In the present study, the effluents streams of municipal biowaste anaerobic digestion, were used to grow methane oxidising bacteria which can be used as protein source. Results demonstrated that a 40:60 CH4:O2 (v/v) gas feeding resulted in microbial biomass production of 0.95 g-DM/L by a Methylophilus dominated community. When raw biogas was used as input for methane corresponding to the same initial methane partial pressure as before, instead of pure methane, the growth was partially hindered (0.61 g-DM/L) due to the presence of H2S (IC50: 1376 ppm). Hence, desulfurization is suggested before using biogas for microbial protein production. At semi-continuous mode, results showed that the produced biomass had relatively high protein content (>40% of dry weight) and the essential amino acids lysine, valine, leucine and histidine were detected at high levels.

A Low-Cost Ecofriendly Oxidation Process to Manufacture High-Performance Polymeric Biosurfactants Derived from Municipal Biowaste.

Biosurfactants account for about 12% of the global value of the surfactant market, which is currently dominated by synthetic surfactants obtained from fossil sources. Yet, the production of biosurfactants from renewable feedstock is bound to increase, driven by the increasing pressure from both society and governments for chemistry-based industries to become more ecofriendly and economically sustainable. A photo-chemical oxidation process is reported here, yielding new biosurfactants from urban biowaste in water that perform as a solvent and terminal oxidant reagent at room temperature without the addition of conventional oxidants and catalysts. Products with 200-500 kDa molecular weight are obtained. They lower the surface tension of water down to 34 mN/m at 0.5-2 g/L concentration. The estimated cost is rather low (0.1-1.5 EUR/kg), which is competitive with the cost of synthetic surfactants but much lower than the cost of the best-performing bacterial surfactants. For the implementation of the photo-chemical oxidation process at the industrial level, the results suggest that the new biosurfactants obtained in the present work may not reach the performance level of the best-performing bacterial surfactants capable of lowering the surface tension of water down to 28 mN/m. Yet, the biosurfactants produced by the photo-chemical process have a greater chance of being marketed on large scales.

Emission of odor pollutants and variation in microbial community during the initial decomposition stage of municipal biowaste.

Odor pollution often occurs in the initial decomposition stage of municipal biowaste, including throwing/collection and transportation. However, this aspect of odor impact from municipal biowaste has not been well studied. In this study, a practical dustbin (120 L) equipped with flux chamber and filled with three types of municipal biowaste was used to simulate garbage storage conditions. The result indicated that the emission rate of odor pollutants for uncooked food waste (UFW) represented a nearly linear growth trend, reaching the maximum (3963 ± 149 µg kg-1 DM h-1) at 72 h. Cooked food waste (CFW) increased rapidly from 8 h to 24 h, and then remain fluctuated, reached the maximum (2026 ± 77 µg kg-1 DM h-1) at 72 h. Comparatively, household kitchen waste (HKW) reached the maximum emission rate (10,396 ± 363 µg kg-1 DM h-1) at 16 h. Sulfide and aldehydes ketones were identified as dominant odor contributor to UFW and CFW, respectively. While aldehydes ketones and sulfides were both dominant odor contributor to HKW. Moreover, the microbial diversity analysis suggests that Acinetobacter was the dominant genus in UFW, and Lactobacillus was the dominant genus in CFW and HKW. In addition, it was evident that each odorous pollutant was significantly associated with two or more bacterial genera, and most bacterial genera such as Acinetobacter, were also significantly associated with multiple odorous pollutants. The variation of odorants composition kept consistent with microbial composition. The present study could provide essential evidence for a comprehensive understanding of odorant generation in the initial decomposition stage of municipal biowaste. It could contribute to setting out strategies for odor control and abatement in municipal biowaste management systems.

Microplastic contamination of soil: Are input pathways by compost overridden by littering?

Compost application is a widely recommended agricultural practice to improve soil fertility. As almost all compost is likely polluted with plastic we hypothesize that compost application is a major input pathway for microplastics (MPs) into agricultural soil. To attribute the plastic load of soil to compost application, we investigated MPs in topsoil (0-30 cm) of a controlled, long-term fertilizer trial with application of compost made of municipal biowaste (0, 5, 10, and 20 t ha-1a-1), which ended 11 years ago. Microplastics were analyzed via density separation (ZnCl2) and light microscopy; testing this method recovered 92 ± 10 % of spiked plastic items. The fields of the long-term compost trial showed a MP load of 0-64 items kg-1, corresponding to MP stocks in the plough layer (0-30 cm) that ranged from 38.2 ± 55.5 million to 171.4 ± 57.5 million items ha-1. Microplastic stocks and contents increased with increasing amount of compost application. Thus, we confirm compost as a major input pathway for MPs into agricultural soil, with the effect still visible after 11 years. Comparison of calculated plastic input based on MP contents of recent German compost with MP loads found in soil revealed that overall compost application explained <6 % of total MP stocks. We assume that compost applied in earlier days contained higher plastic loads than recent ones, reflecting current awareness and successful efforts in reducing plastic loads during compost production. However, as the plots at the border of the field had up to 18 times higher MP loads than the inner plots of the trial, we suggest that littering also contributed significantly to MP pollution. Thus, even if given compost applications still add plastics to environment, other sources such as littering can already have become the dominating input pathway.

Methane potential from municipal biowaste: Insights from six communities in Maharashtra, India.

Anaerobic digestion (AD) of biowaste can generate biogas with methane (CH4) as energy source and contribute to sustainable municipal solid waste management in India. Characteristic municipal biowastes sampled seasonally from household, fruit and vegetable market and agricultural waste collection points in villages, towns and cities in Maharashtra were analysed to assess the potential as substrate for AD. The mean biochemical methane potential (BMP, at 37 °C) across seasons and community sizes was between 200-260, 175-240 and 101-286 NLCH4 kgvs-1 for household, market and agricultural biowaste, respectively. CH4 yields were comparable in villages, towns and cities. Seasonal variations in CH4 yields were observed for market and agricultural biowaste with highest values during pre-monsoon season. Results underpin that municipal biowaste is a suitable substrate for AD in India. However, low purity of available biowaste resulted in lower CH4 yields compared to recent studies using source-segregated biowaste.