Different inocula produce distinctive microbial consortia with similar lignocellulose degradation capacity.

Despite multiple research efforts, the current strategies for exploitation of lignocellulosic plant matter are still far from optimal, being hampered mostly by the difficulty of degrading the recalcitrant parts. An interesting approach is to use lignocellulose-degrading microbial communities by using different environmental sources of microbial inocula. However, it remains unclear whether the inoculum source matters for the degradation process. Here, we addressed this question by verifying the lignocellulose degradation potential of wheat (Triticum aestivum) straw by microbial consortia generated from three different microbial inoculum sources, i.e., forest soil, canal sediment and decaying wood. We selected these consortia through ten sequential-batch enrichments by dilution-to-stimulation using wheat straw as the sole carbon source. We monitored the changes in microbial composition and abundance, as well as their associated degradation capacity and enzymatic activities. Overall, the microbial consortia developed well on the substrate, with progressively-decreasing net average generation times. Each final consortium encompassed bacterial/fungal communities that were distinct in composition but functionally similar, as they all revealed high substrate degradation activities. However, we did find significant differences in the metabolic diversities per consortium: in wood-derived consortia cellobiohydrolases prevailed, in soil-derived ones ß-glucosidases, and in sediment-derived ones several activities. Isolates recovered from the consortia showed considerable metabolic diversities across the consortia. This confirmed that, although the overall lignocellulose degradation was similar, each consortium had a unique enzyme activity pattern. Clearly, inoculum source was the key determinant of the composition of the final microbial degrader consortia, yet with varying enzyme activities. Hence, in accord with Beyerinck's, "everything is everywhere, the environment selects" the source determines consortium composition.

Microbial electrolysis cell coupled with anaerobic granular sludge: A novel technology for real bilge water treatment.

In the current study, treatment of undiluted real bilge water (BW) and the production of methane was examined for the first time using a membraneless single chamber Microbial Electrolysis Cell (MEC) with Anaerobic Granular Sludge (AGS) for its biodegradation. Initially, Anaerobic Toxicity Assays (ATAs) were used to evaluate the effect of undiluted real BW on the methanogenic activity of AGS. According to the results, BW shown higher impact to acetoclastics compared to hydrogenotrophic methanogens which proved to be more tolerant. However, dilution of BW caused lower inhibition allowing BW biodegradation. Maximum methane production (142.2 ± 4.8 mL) was observed at 50% of BW. Operation of MEC coupled with AGS, seemed to be very promising technology for BW treatment. During 80 days of operation in increasing levels of BW, R2 (1 V) reactor resulted in better performance than AGS alone. Exposure of AGS to gradual increase of BW content revealed that CH4 production was possible and reached 51% in five days even after feeding with 90% of BW using simple commercial iron electrodes. Successful chemical oxygen demand (sCOD) removal (up to 70%) was observed after gradual biomass acclimatization. Among the different monitored volatile fatty acids (VFAs), acetic and valeric acids were the most frequently detected compounds with concentrations up to 2.79 and 1.81 g L-1, respectively. The recalcitrant nature of BW did not allow the MEC-AD (anaerobic digester) to balance the consumed energy. Microbial profile analysis confirmed the existence of several methanogenic microorganisms of which Desulfovibrio and Methanobacterium presented significantly higher abundance in the cathodes compared to anodes and AGS.