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Plant Biotechnol J ; 15(5): 634-647, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-27862876

RESUMO

The extreme sensitivity of the microsporogenesis process to moderately high or low temperatures is a major hindrance for tomato (Solanum lycopersicum) sexual reproduction and hence year-round cropping. Consequently, breeding for parthenocarpy, namely, fertilization-independent fruit set, is considered a valuable goal especially for maintaining sustainable agriculture in the face of global warming. A mutant capable of setting high-quality seedless (parthenocarpic) fruit was found following a screen of EMS-mutagenized tomato population for yielding under heat stress. Next-generation sequencing followed by marker-assisted mapping and CRISPR/Cas9 gene knockout confirmed that a mutation in SlAGAMOUS-LIKE 6 (SlAGL6) was responsible for the parthenocarpic phenotype. The mutant is capable of fruit production under heat stress conditions that severely hamper fertilization-dependent fruit set. Different from other tomato recessive monogenic mutants for parthenocarpy, Slagl6 mutations impose no homeotic changes, the seedless fruits are of normal weight and shape, pollen viability is unaffected, and sexual reproduction capacity is maintained, thus making Slagl6 an attractive gene for facultative parthenocarpy. The characteristics of the analysed mutant combined with the gene's mode of expression imply SlAGL6 as a key regulator of the transition between the state of 'ovary arrest' imposed towards anthesis and the fertilization-triggered fruit set.


Assuntos
Frutas/genética , Proteínas de Plantas/genética , Solanum lycopersicum/genética , Sistemas CRISPR-Cas , Frutas/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Resposta ao Choque Térmico/genética , Solanum lycopersicum/fisiologia , Mutação , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Sementes/genética
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