Identification of leader-trailer helices of precursor ribosomal RNA in all phyla of bacteria and archaea.

Ribosomal RNAs are transcribed as part of larger precursor molecules. In Escherichia coli, complementary RNA segments flank each rRNA and form long leader-trailer (LT) helices, which are crucial for subunit biogenesis in the cell. A previous study of 15 representative species suggested that most but not all prokaryotes contain LT helices. Here, we use a combination of in silico folding and covariation methods to identify and characterize LT helices in 4464 bacterial and 260 archaeal organisms. Our results suggest that LT helices are present in all phyla, including Deinococcota, which had previously been suspected to lack LT helices. In very few organisms, our pipeline failed to detect LT helices for both 16S and 23S rRNA. However, a closer case-by-case look revealed that LT helices are indeed present but escaped initial detection. Over 3600 secondary structure models, many well supported by nucleotide covariation, were generated. These structures show a high degree of diversity. Yet, all exhibit extensive base-pairing between the leader and trailer strands, in line with a common and essential function.

tRNA-like leader-trailer interaction promotes 3'-end maturation of MALAT1.

Human metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a nuclear long noncoding RNA (lncRNA) that is highly overexpressed in many cancer tissues and plays important roles in tumor progression and metastasis. The MALAT1 primary transcript contains evolutionarily conserved structural elements in its 3'-terminal region: a triple helix forming element called element for nuclear expression (ENE) and a downstream tRNA-like structure called mascRNA. Instead of being polyadenylated, mature MALAT1 is generated by recognition and processing of the mascRNA by RNase P. A genomically encoded A-rich tract at the new 3' end of MALAT1, which is generated upon RNase P cleavage, forms a triple helical structure with the upstream ENE. Triplex formation is vital for stabilization of the mature transcript and for subsequent accumulation and oncogenic activity of MALAT1. Here, we demonstrate that efficient 3'-end maturation of MALAT1 is dependent on an interaction between the A-rich tract and the mascRNA 3' trailer. Using mutational analyses of cell-based reporter accumulation, we show that an extended mascRNA acceptor stem and formation of a single bulged A 5' to the RNase P cleavage site are required for efficient maturation of the nascent MALAT1 3' end. Our results should benefit the development of therapeutic approaches to cancer through targeting MALAT1.

Hardware-Efficient Scheme for Trailer Robot Parking by Truck Robot in an Indoor Environment with Rendezvous.

Autonomous grounded vehicle-based social assistance/service robot parking in an indoor environment is an exciting challenge in urban cities. There are few efficient methods for parking multi-robot/agent teams in an unknown indoor environment. The primary objective of autonomous multi-robot/agent teams is to establish synchronization between them and to stay in behavioral control when static and when in motion. In this regard, the proposed hardware-efficient algorithm addresses the parking of a trailer (follower) robot in indoor environments by a truck (leader) robot with a rendezvous approach. In the process of parking, initial rendezvous behavioral control between the truck and trailer robots is established. Next, the parking space in the environment is estimated by the truck robot, and the trailer robot parks under the supervision of the truck robot. The proposed behavioral control mechanisms were executed between heterogenous-type computational-based robots. Optimized sensors were used for traversing and the execution of the parking methods. The truck robot leads, and the trailer robot mimics the actions in the execution of path planning and parking. The truck robot was integrated with FPGA (Xilinx Zynq XC7Z020-CLG484-1), and the trailer was integrated with Arduino UNO computing devices; this heterogenous modeling is adequate in the execution of trailer parking by a truck. The hardware schemes were developed using Verilog HDL for the FPGA (truck)-based robot and Python for the Arduino (trailer)-based robot.

Regulated emission characteristics of in-use LNG and diesel semi-trailer towing vehicles under real driving conditions using PEMS.

On-road driving emissions of six liquefied natural gas (LNG) and diesel semi-trailer towing vehicles (STTVs) which met China Emission Standard IV and V were tested using Portable Emission Measurement System (PEMS) in northern China. Emission characteristics of these vehicles under real driving conditions were analyzed and proved that on-road emissions of heavy-duty vehicles (HDVs) were underestimated in the past. There were large differences among LNG and diesel vehicles, which also existed between China V vehicles and China IV vehicles. Emission factors showed the highest level under real driving conditions, which probably be caused by frequent acceleration, deceleration, and start-stop. NOx emission factors ranged from 2.855 to 20.939 g/km based on distance-traveled and 6.719-90.557 g/kg based on fuel consumption during whole tests, which were much higher than previous researches on chassis dynamometer. It was inferred from tests that the fuel consumption rate of the test vehicles had a strong correlation with NOx emission, and the exhaust temperature also affected the efficiency of Selected Catalytic Reduction (SCR) after-treatment system, thus changing the NOx emission greatly. THC emission factors of LNG vehicles were 2.012-10.636 g/km, which were much higher than that of diesel vehicles (0.029-0.185 g/km). Unburned CH4 may be an important reason for this phenomenon. Further on-road emission tests, especially CH4 emission test should be carried out in subsequent research. In addition, the Particulate Number (PN) emission factors of diesel vehicles were at a very high level during whole tests, and Diesel Particulate Filter (DPF) should be installed to reduce PN emission.

Shep interacts with posttranscriptional regulators to control dendrite morphogenesis in sensory neurons.

RNA binding proteins (RBPs) mediate posttranscriptional gene regulatory events throughout development. During neurogenesis, many RBPs are required for proper dendrite morphogenesis within Drosophila sensory neurons. Despite their fundamental role in neuronal morphogenesis, little is known about the molecular mechanisms in which most RBPs participate during neurogenesis. In Drosophila, alan shepard (shep) encodes a highly conserved RBP that regulates dendrite morphogenesis in sensory neurons. Moreover, the C. elegans ortholog sup-26 has also been implicated in sensory neuron dendrite morphogenesis. Nonetheless, the molecular mechanism by which Shep/SUP-26 regulate dendrite development is not understood. Here we show that Shep interacts with the RBPs Trailer Hitch (Tral), Ypsilon schachtel (Yps), Belle (Bel), and Poly(A)-Binding Protein (PABP), to direct dendrite morphogenesis in Drosophila sensory neurons. Moreover, we identify a conserved set of Shep/SUP-26 target RNAs that include regulators of cell signaling, posttranscriptional gene regulators, and known regulators of dendrite development.

Zfrp8 forms a complex with fragile-X mental retardation protein and regulates its localization and function.

Fragile-X syndrome is the most commonly inherited cause of autism and mental disabilities. The Fmr1 (Fragile-X Mental Retardation 1) gene is essential in humans and Drosophila for the maintenance of neural stem cells, and Fmr1 loss results in neurological and reproductive developmental defects in humans and flies. FMRP (Fragile-X Mental Retardation Protein) is a nucleo-cytoplasmic shuttling protein, involved in mRNA silencing and translational repression. Both Zfrp8 and Fmr1 have essential functions in the Drosophila ovary. In this study, we identified FMRP, Nufip (Nuclear Fragile-X Mental Retardation Protein-interacting Protein) and Tral (Trailer Hitch) as components of a Zfrp8 protein complex. We show that Zfrp8 is required in the nucleus, and controls localization of FMRP in the cytoplasm. In addition, we demonstrate that Zfrp8 genetically interacts with Fmr1 and tral in an antagonistic manner. Zfrp8 and FMRP both control heterochromatin packaging, also in opposite ways. We propose that Zfrp8 functions as a chaperone, controlling protein complexes involved in RNA processing in the nucleus.

Smoking status, dental visits and receipt of tobacco counseling in dental office among mobile and trailer home adolescents.

BACKGROUND: Mobile and trailer home (MTHs) residents are an understudied group. In this study we determined the cigarette smoking status, dental visits in the past 12 months, and receipt of tobacco counseling in adolescents living in MTHs compared to adolescents living in other types of housing. METHODS: For this secondary data analysis study, we used data of adolescents aged 10 to 19 years (n = 74,890) from the 2012 Florida Youth Tobacco Survey (FYTS). Weighted multiple logistic regression model was conducted to understand the differences between adolescents living in MTHs compared to those living in other types of housing. RESULTS: Approximately 6 % of the sample reported living in MTHs. The regression model showed that older (p < 0.0001), female (p = 0.0091), and middle school (p < 0.0001) adolescents were more likely, and those who identified as Asians (p = 0.0006), Black/African Americans (p < 0.0001), and Hispanics (p < 0.0001) were less likely to be living in MTHs compared to their counterparts. Current established smokers (p < 0.0001) and non-established smokers (p < 0.0001) were more likely to report living in MTHs compared to non-smokers. Those reporting to have not visited a dental office (p < 0.0001) were more likely to be living in MTHs. Those who visited a dental office but not received any tobacco counseling (p < 0.0001) were less likely to be living in MTHs compared to their counterparts. CONCLUSIONS: Current cigarette smokers and those not visiting a dental office were more likely to be MTH adolescents. Adolescents reporting to have received tobacco counseling in a dental office were more likely to be living in MTHs.

Mobile imaging trailers: A scoping review of CT and MRI modalities.

INTRODUCTION: Mobile Imaging Trailers enable moving diagnostic imaging equipment between locations requiring very little setup and configuration, example given CT-scanners and MRI-scanners. However, despite the apparent benefits of utilising these imaging capabilities, very little research on the subject exists. This study aims at gaining an overview of the current state of the literature, using the scoping review methodology. METHODS: The systematic literature search was conducted in three databases: Scopus, Embase and PubMed. Included sources were extracted based on the objectives of the scoping review, and inspired by the by PRISMA-ScR. RESULTS: 29 papers were included. CONCLUSION: The results of the review showed that three general categories of research on this subject exist - trailers used in research, trailers as the object of research and trailers as an element or tool of the research. Of these, the most prevalent one used is the latter - trailers used as an element or tool of the research. This; however, is an issue for the use of trailers in a clinical setting, as very little research has been conducted on how they might be used and how they compare to fixed installations. As seen during the recent COVID-19 pandemic, the potentials for the use of MITs are immense; however, with the current lack of knowledge and understanding, the full potential has not been realised, suggesting further research should be focused in this area. IMPLICATIONS FOR PRACTICE: This study has shown that the limited research in the area does point towards a few benefits of MITs; however, there is a clear lack of sufficient research on the field to say this with confidence.

Evaluation of ATP bioluminescence for rapid determination of cleanliness of livestock trailers after a commercial wash.

Pathogens such as porcine epidemic diarrhea virus (PEDV), porcine reproductive and respiratory syndrome (PRRSV), and E. coli are known to spread by contaminated vehicles and equipment. Pork producers have adopted trailer wash policies where each trailer is washed, disinfected, and dried before it can return to a farm. Cleanliness of livestock trailers after washing is determined by visual inspection rather than any objective method. Adenosine triphosphate (ATP) bioluminescence is used in many industries to provide real-time feedback on surface cleanliness through the detection of ATP from organic sources. That same technology may provide trailer wash facilities a way of objectively characterizing a livestock trailer's suitability to return to a farm after washing. Two ATP luminometers (3M Clean-Trace and Neogen AccuPoint) were used to estimate the correlation between ATP bioluminescence readings and aerobic bacterial plate counts (APCs) from sampled surfaces and to determine locations within a livestock trailer that can accurately estimate surface cleanliness. Five locations in livestock trailers were evaluated. Those locations included the nose access door (NAD), back door flush gate, rear side access door (RSAD), belly flush gate (BFG), and belly side access door (BSAD). There was a positive log-log association between the two luminometers (r = 0.59, P < 0.01). Every log unit increase in one unit, resulted in a 0.42 log increase (P < 0.01) in the other unit. ATP can come from bacteria, yeasts, molds, and manure. There was a poor association (r ≥ 0.10, P ≥ 0.02) between APCs and the ATP luminometers. Still, an increase in relative light units (RLUs) resulted in a corresponding increase in colony-forming units. The greatest area of surface contamination measured by APC was the NAD. RLUs were also greater in the NAD compared to the RSAD, the BFG, and the BSAD (P ≤ 0.01). Because APCs and luminometer RLUs provided similar outcomes, statistical process control charts were developed to determine control limits for RLUs. This provides real-time feedback to trailer wash workers in determining cleanliness outcomes for livestock trailers. These data suggest that ATP bioluminescence can be a reliable method to monitor cleaning effectiveness in livestock trailers. Bioluminescence is a monitoring tool that should be used in conjunction with microbial methods to monitor procedures for cleaning and disinfection.

Backstepping based intelligent control of tractor-trailer mobile manipulators with wheel slip consideration.

In this research, a new hybrid backstepping control strategy based on a neural network is proposed for tractor-trailer mobile manipulators in the presence of unknown wheel slippage and disturbances. To minimize the negative impacts of wheel slippage, the desired velocities of the tractor's wheels are computed with a proposed kinematic control model with an adaptive term. As the system's dynamical model contains unavoidable uncertainties, model-based backstepping control technique is unable to effectively manage these systems. Hence, the proposed controller blends a radial basis function neural network with the merits of a dynamical model-based backstepping approach. The neural networks are employed to approximate the non-linear unknown smooth function. To minimize the impact of external disturbances, and network reconstruction error an adaptive term is added to the control law. The Lyapunov theorem and Barbalat's lemma are employed to guarantee the stability of the control method. The tracking error is shown to be bounded and to rapidly converge to zero with the proposed method. To demonstrate the efficacy and validity of the control mechanism, comparison simulation results are presented.

Dynamics modeling and path following controller of tractor-trailer-wheeled robots considering wheels slip.

To improve the payload capacity and maneuverability of a Differentially-Driven Wheeled Robot (DDWR), a wheeled vehicle, which is called trailer, is connected to the DDWR. In all of the previous studies of DDWRs with a trailer, the robot wheels are subject to pure rolling constraints. However, when these multibody systems move with high velocities/accelerations, transfer a heavy payload, or travel on a slippery surface, they experience slipping and/or skidding. In the present study, a Tractor-Trailer Wheeled Mobile Robot (TTWMR) is modeled whose wheels may slip in lateral and longitudinal directions. To this end, Lagrange formalism is employed along with the LuGre friction model to derive dynamics of the considered multibody system. Next, the problem of path following for the trailer is addressed. Toward this goal, the partial feedback linearization technique will be utilized. The obtained simulation results prove the superiority of the performance of the suggested method in comparison to the previous studies. Additionally, the response of the system in the presence of the external disturbances and uncertainties in system parameters will be examined.

Habituation to Livestock Trailer and Its Influence on Stress Responses during Transportation in Goats.

This experiment was conducted to determine the effects of habituation to livestock trailers on stress responses in goats transported for long periods. Intact male Spanish goats (12-month old; BW = 31.6 ± 0.34 kg; N = 168) were separated into two treatment (TRT) groups and maintained on two different paddocks. Concentrate supplement was fed to one group inside two livestock trailers (5.0 × 2.3 m each; habituated group, H), while the other group received the concentrate supplement, but not inside the trailers (non-habituated, NH). After 4 weeks of habituation period, goats were subjected to a 10-h transportation stress in four replicates (n = 21 goats/replicate/TRT). Blood samples were collected by a trained individual by jugular venipuncture into vacutainer tubes before loading (Preload), 20 min after loading (0 h), and at 2-h intervals thereafter (Time) for analysis of stress responses. There was a tendency for a TRT effect (p < 0.1) on tyramine and metanephrine concentrations. Phenylethylamine and 5-methoxytryptamine concentrations were significantly greater (p < 0.05) in the H group compared to the NH group. Both dopamine and 5-methoxytryptamine concentrations decreased (p < 0.05) with transportation time; however, TRT × Time interaction effects were not significant. Habituation to trailers may be beneficial in mood and energy stabilization in goats during long-distance transportation.

The promotion of critical reading through the digital environment: A study on the virtual epitexts used to promote children's picturebooks.

The most recent research studies in the field of reading describe a new cultural ecosystem in which analog and digital reading coexist and contribute to transform what is read, either through the way reading is performed or by promoting reading. In this context, the training of critical readers is particularly important, an aspect emphasized by UNESCO and the curriculum frameworks based on its premises. In order to provide data for reflection on this question, this paper presents an essentially qualitative and interpretive documentary study of a sample of 836 virtual epitexts that promote children's picturebooks. The selected documents consist of the postings by 45 publishing houses between 2020 and 2022 on their YouTube and Vimeo channels. The results of the content analysis present the current tendencies in digital promotion of children's books and the strategies most likely to encourage critical reading. The insistence on the author's presence, the emphasis on the materiality of the book as an object, the strengthening of artistic discourse and the hybridization of reality and fiction, among other aspects, all propose a type of reading that favors the development of critical thinking. The results are complemented by a selection of virtual epitexts suggested to mediators and readers as resources of interest in promoting critical reading in socio-educational contexts.

Significance of tractor-trailer impacts to the rear of light vehicles.

OBJECTIVE: This study determined the type of striking vehicle or object associated with serious injury to at least 1 occupant in the vehicle struck in the rear. METHOD: The 1990-2015 NASS-CDS was analyzed for light vehicles involved in rear crashes. The type of vehicle struck in the rear resulting in serious injury to at least 1 occupant was determined. Rear impacts were identified with GAD1 = B without rollover (rollover ≤ 0). Vehicles with serious to maximum injury were identified as VAIS 3 to 6 (VAIS 3+). The type of striking vehicle or object was determined for the rear impacts causing serious injury. The risk and relative risk for serious injury were determined. Review of electronic cases was conducted for light vehicles with serious injury (VAIS 3+) in impacts by tractor-trailers or large trucks. RESULTS: The highest risk for serious injury to an occupant in the struck vehicle was from a tractor-trailer or large truck (2.71%), followed by a fixed object (1.49%) and van (1.00%). The average risk was 0.33%. The relative risk was 8.2 times for impact with a tractor-trailer or large truck and 4.5 times for impact with a fixed object. The highest risk impacts with a fixed object were rear impacts into a tree/bush (3.57%) and pole (2.90%). Tractor-trailers and large trucks accounted for 16.2% of serious injury in struck vehicles, followed by fixed objects at 12.9%; the 2 accounted for 29.1% of serious injury to occupants of struck vehicles. The case review identified 22 tractor-trailer and 1 large truck crashes involving 31 seriously injured occupants in the struck vehicle. There were 2 general crash scenarios associated with serious injury. One involved intrusion deforming the occupant compartment. The other involved vehicle accelerations sufficient to seriously injure an occupant in the struck vehicle without significant deformation to the occupant compartment. CONCLUSION: This study provides insight into the striking vehicle or object associated with serious injury to light vehicle occupants in rear impacts. Tractor-trailer impacts into the rear of a vehicle involved a high risk for serious injury in the struck vehicle. The use of collision mitigation technologies, such as forward collision warning or automatic emergency braking, on the tractor-trailer may support better speed control and lead to lower closing velocities of rear impacts to light vehicles.

Novel avian orthoavulavirus 13 in wild migratory waterfowl: biological and genetic considerations.

Avian orthoavulavirus 13 (AOAV-13), formerly known as Avian paramyxovirus 13 (APMV-13), is found scatteredly in wild birds around the world. Although four complete genome sequences of AOAV-13 had been identified since the first discovery in Japan in 2003, the information available on the genetic variation and biological characteristics of AOAV-13 is still limited. In the present study, we isolated six AOAV-13 strains from fecal samples of wild migratory waterfowls during annual (2014-2018) viral surveillance of wild bird populations from wetland and domestic poultry of live bird markets (LBMs) in China. The phylogenetic analyses based on the HN and F genes showed that they had very close relationship and the molecular clock estimations showed a low evolutionary rate of AOAV-13. However, Bean goose/Hubei/V97-1/2015 is 1953 nt in size (ORF, 1, 776 nt), which is a unique size and longer than other reported AOAV-13 strains. Additionally, four repeats of conserved sequences "AAAAAT" was presented in the 5'-end trailer region of Swan goose/Hubei/VI49-1/2016, which is unprecedented in the AOAV-13. These findings highlight the importance of continuous monitoring the specific species of APMVs.

Dataset for trailer angle estimation using radar point clouds.

The automotive industry is interested in the estimation of vehicle trailer rotation (trailer angle or hitch angle) due to its use in trailer control algorithms. We present an experimental dataset collected in a study of the estimation problem [1] and a MATLAB code implementation of the study. The data collection apparatus is a truck mock-up that is attached to a flatbed trailer at the hitch ball. Two radars are installed in the taillight fixtures of the truck and a camera is installed in the truck's tailgate like a typical backup camera installation. A rotary motion sensor is also installed at the hitch ball to provide ground truth measurement of the trailer angle. To aid analysis of the dataset, both radar detections are transformed onto a vehicle coordinate system (VCS) having its origin at the hitch ball i.e. the different radar viewpoints are combined into one with respect to the hitch ball. The MATLAB code presented with this article has two major functionalities. The first functionality is the visualization of both radar detections, the combined radar detections in the VCS, the camera images, and the ground truth angles, as the trailer rotates. The second functionality of the code is the replication of the estimation results in [1], which used only the radar detections from the dataset.

A Novel Load Cell-Supported Research Platform to Measure Vertical and Horizontal Motion of a Horse's Centre of Mass During Trailer Transport.

During transport, horses are subjected to acceleration in three dimensions, rapid braking, turning, noise, and other stressors. The animal's ability to make postural corrections may be insufficient to prevent injury or distress, and so knowledge of the compensatory motion patterns of the horse in the trailer is a necessary precondition for smart design of transport systems. A custom two-horse trailer was built for this project. It had a horse compartment 1.85 m wide by 3.95 m long, with adjustable bulkheads and a centre divider separating the horses. The floor was instrumented with 24 shearbeam load cells to measure the vertical load imposed by each horse and its horizontal motion. Two horses were driven on a 56 km trip on both rural and urban roads. Load data were collected at 100 Hz for the 58-minute trip and were filtered with a cut-off frequency of 5 Hz using a Butterworth low-pass filter and then vertical acceleration computed. A pivot table counted sign reversals in the vertical acceleration signal, and vertical displacement was calculated using the fundamental frequency of the resulting acceleration data. Total vertical motion was calculated by making the negative displacements absolute and summing these with the positive displacements, and vertical work done was calculated by multiplying the force by the displacement measures. Horizontal motion was calculated by averaging the transverse and cranio-caudal position of the centre of pressure every second and adding the resultant displacements. Absolute vertical displacement of the two horses was 69.55 m and 97.56 m. In addition to the work done by standing, vertical work done in response to vibration was 322.4 kJ and 443.2 kJ. Horizontal excursion was 227.1 m and 243.0 m. This is a first effort to quantify the additional workload imposed on animals during transport, which will aid in the design of smart transport vehicles that will minimize the stress to horses.

Effects of Vehicle Load on Emissions of Heavy-Duty Diesel Trucks: A Study Based on Real-World Data.

Vehicle loads have significant impacts on the emissions of heavy-duty trucks, even in the same traffic conditions. Few studies exist covering the differences in emissions of diesel semi-trailer towing trucks (DSTTTs) with different loads, although these vehicles have a wide load range. In this context, the operating modes and emission rates of DSTTTs were analyzed under varying loads scenarios to understand the effect of vehicle loads on emission factors. First, second-by-second field speed data and emission data of DSTTTs with different loads were collected. Then, the methods for calculating the scaled tractive power (STP) and the emissions model for DSTTTs were proposed to evaluate the effect of different loading scenarios. The STP distributions, emission rate distributions, and emission factor characteristics of different loaded trucks were analyzed and compared. The results indicated that the STP distributions of DSTTTs that under the unloaded state were more narrow than those under fully loaded or overloaded conditions. The emission rates of carbon dioxide (CO2), carbon monoxide (CO) and total hydrocarbon (THC) for DSTTTs under a fully loaded state were significantly higher than those under an unloaded state. However, due to the influence of exhaust temperature, the emission rates of nitrogen oxides (NOx) among fully loaded trucks were lower than those under the unloaded state when STP bin was above 4 kW/ton. The emission factors of CO2, CO, THC, and NOx for fully loaded trucks demonstrated the largest increases at low-speed intervals (0-30 km/h), which rose by 96.2%, 47.9%, 27.8%, and 65.2%, respectively.

Precise Temporal Regulation of Post-transcriptional Repressors Is Required for an Orderly Drosophila Maternal-to-Zygotic Transition.

In animal embryos, the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents more than half of the protein-coding capacity of Drosophila melanogaster's genome, and that 2% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). Although the ubiquitin-proteasome system is necessary for clearance of these repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL, and ME31B for degradation early in the MZT and the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG abrogates F-box protein interaction and confers immunity to degradation. Persistent SMG downregulates zygotic re-expression of mRNAs whose maternal contribution is degraded by SMG. Thus, clearance of SMG permits an orderly MZT.

A Retrospective Survey of Factors Affecting the Risk of Incidents and Equine Injury During Non-Commercial Transportation by Road in the United Kingdom.

The number of equines injured as a result of incidents during road transport is currently unknown in the United Kingdom. Although previous research has identified factors that affect an equine's behavioural and physiological responses to transportation, their contribution to incident occurrence and injury risk is unclear. The aim of this study was to identify factors associated with incident occurrence and equine injury during transportation by road. An online survey was administered between 12th May 2017 and 21st July 2017 in the UK. The survey was open to those transporting equines non-commercially and comprised two sections. Questions relating to general transport behaviour were completed by all participants. Participants who had experienced an incident then provided details of these, including outcomes. Incidents were reported by 16.2% (342/2116) of participants, with details included for 399 incidents. Those participants who had a professional/competitive involvement with equines reported more incidents than those with a predominantly leisure involvement (p < 0.01). Equine behaviour was the attributed cause of 56% of incidents reported and most incidents occurred during the first hour of travel (65%). In over 50% of the incidents reported, the equine was injured, with those incidents attributed to transport vehicle malfunction being associated with the highest percentage of injury (68%). This study highlights the need for better preparation of the equine for transportation and to identify risk factors associated with transport vehicle type, design and operation.