Monitoring tree canopy dynamics across heterogeneous urban habitats: A longitudinal study using multi-source remote sensing data.

Urban trees have attracted increasing attention to serve as a green prescription for addressing various challenges facing human society like climate change and environmental deterioration. However, without healthy growth of urban trees, they cannot service any environmental, social, and economic benefits in a sustainable manner. By monitoring the canopy development, the tree growth dynamics in different urban habitats can be detected and appropriate management approaches can be executed. Using the Kowloon Peninsula, Hong Kong, as a case, this study explores how remote sensing data can help monitor and understand the impacts of heterogeneous urban habitats on tree canopy dynamics. Four algorithms based on WorldView-2 satellite image are compared to optimize the canopy segmentation. Then the individual tree canopy is integrated with Sentinel-2 satellite data to obtain canopy growth dynamics for each season from 2016 to 2020. Three indicators are applied to reflect tree canopy status, including the fluorescence correction vegetation index (FCVI, tracking leaf chlorophyll density), the soil adjusted total vegetation index (SATVI, measuring the density of woody branches and twigs), and the normalised difference phenology index (NDPI, capturing canopy water content). And four heterogeneous habitats where urban trees stand are specified. The results revealed that urban trees show varying canopy growth status, in a descending order from natural terrains, parks, residential lands, to road verges, suggesting that urban habitats curtail trees' growth significantly. Additionally, two super-typhoons in 2017 and 2018, respectively, caused serious damages to tree canopy. Relevant resiliency of tree varies, echoing the sequence of canopy growth status with those in road verges the least resilient. This study shows how remote sensing data can be used to provide a better understanding of long-term tree canopy dynamics across large-scale heterogeneous urban habitats, which is key to monitoring and maintaining the health and growth of urban trees.

Molecular characterization of Schellackia parasites in an urban population of sand lizards (Lacerta agilis) from Berlin, Germany.

Lizards are hosts of several taxa of unicellular parasites of the phylum Apicomplexa, including Karyolysus, Schellackia, Lankesterella, and Hepatozoon. Parasite prevalence and the impact of infections on lizard biology remain largely unexplored. In this study, blood parasite infections were investigated in sand lizards (Lacerta agilis) from Berlin, Germany. Eighty-three individuals were investigated, and the detected blood parasites were identified as Schellackia sp. The combination of microscopic and molecular screening revealed a prevalence of 14.5%. Parasitemia values were low and most infections were subpatent. Phylogenetic analysis recovered a close relationship of the Schellackia parasites of this study with Schellackia sp. parasites of different Lacerta and Podarcis lizard species from Spain. Monitoring of Schellackia parasite infections in free-ranging lizards contributes to a better understanding of the distribution, diversity, and phylogenetic relationships of the neglected parasite taxon.

Helminths of urban rats in developed countries: a systematic review to identify research gaps.

Although black (Rattus rattus) and brown (Rattus norvegicus) rats are among the most widespread synanthropic wild rodents, there is a surprising scarcity of knowledge about their ecology in the urban ecosystem. In particular, relatively few studies have investigated their helminth species diversity in such habitat. We followed the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) guideline to synthesize the existing published literature regarding the helminth fauna of urban rats in developed countries (North America, Europe, Australia, New Zealand and Japan). We aimed at describing the species diversity and richness of urban rat helminths, the species prevalence and associations, the methods of investigation, the pathological changes observed in the hosts, the risk factors of infection and the public health significance of rat-borne helminthiases. Twenty-three scientific papers published between 1946 and 2019 were reviewed, half of them were conducted in Europe. Twenty-five helminth species and eight genera were described from the liver, digestive tract, lungs and muscles of urban rats. The most commonly reported parasite was Calodium hepaticum. Prevalence and risk factors of helminth infection in urban rats varied greatly between studies. Observed pathological findings in the rat host were generally minor, except for C. hepaticum. Several rat helminths can parasitize humans and are therefore of public health significance. The lack of references to identification keys and the rare use of molecular tools for species confirmation represent the main limitation of these studies. Knowledge gap on this topic and the needs for future research are discussed.

A Deep Learning-Based Automatic Mosquito Sensing and Control System for Urban Mosquito Habitats.

Mosquito control is important as mosquitoes are extremely harmful pests that spread various infectious diseases. In this research, we present the preliminary results of an automated system that detects the presence of mosquitoes via image processing using multiple deep learning networks. The Fully Convolutional Network (FCN) and neural network-based regression demonstrated an accuracy of 84%. Meanwhile, the single image classifier demonstrated an accuracy of only 52%. The overall processing time also decreased from 4.64 to 2.47 s compared to the conventional classifying network. After detection, a larvicide made from toxic protein crystals of the Bacillus thuringiensis serotype israelensis bacteria was injected into static water to stop the proliferation of mosquitoes. This system demonstrates a higher efficiency than hunting adult mosquitos while avoiding damage to other insects.

Urban rodent reservoirs of Borrelia spp. in Warsaw, Poland.

The anticipated worldwide surge in urban environments is generating ever-greater interest in the study of host-pathogen interactions in this specific type of habitat. We investigated the potential of city-inhabiting rodents to serve as the main Lyme borreliosis agents (Borrelia spp.) reservoir. We also tried to verify if anthropogenic disturbances changing the vertebrate species community composition may also alter the scheme of Borrelia spp. circulation. A total of 252 Apodemus mice (A. agrarius, A. flavicollis, A. sylvaticus) were captured in Warsaw (Poland), at sites classified into different zones of anthropogenic disturbance, ranging from suburban forests to municipal parks strictly in the city centre. Borrelia spp. infection, ascertained based on bacterium DNA presence in the rodents' blood, was found only in A. agrarius and A. flavicollis (7.6 and 6%, respectively). Only one species from the Borrelia genus - the mammal-associated species B. afzelii - was found in the mice studied. We found no statistical evidence of a correlation between infection in Apodemus mice and the zone of anthropogenic disturbance where the mice were caught. Non-homogeneous concentrations of Borelia spp. infected specimens within the strict city centre area suggest a lack of contact between members of particular mice subpopulations, and their responsibility for relatively high, but local Borrelia spp. infection.

Nest design in a changing world: great tit Parus major nests from a Mediterranean city environment as a case study.

Investigations of urbanization effects on birds have focused mainly on breeding traits expressed after the nest-building stage (e.g. first-egg date, clutch size, breeding success, and offspring characteristics). Urban studies largely ignored how and why the aspects of nest building might be associated with the degree of urbanization. As urban environments are expected to present novel environmental changes relative to rural environments, it is important to evaluate how nest-building behavior is impacted by vegetation modifications associated with urbanization. To examine nest design in a Mediterranean city environment, we allowed urban great tits (Parus major) to breed in nest boxes in areas that differed in local vegetation cover. We found that different measures of nest size or mass were not associated with vegetation cover. In particular, nests located adjacent to streets with lower vegetation cover were not smaller or lighter than nests in parks with higher vegetation cover. Nests adjacent to streets contained more pine needles than nests in parks. In addition, in nests adjacent to streets, nests from boxes attached to pine trees contained more pine needles than nests from boxes attached to other trees. We suggest that urban-related alterations in vegetation cover do not directly impose physical limits on nest size in species that are opportunistic in the selection of nesting material. However, nest composition as reflected in the use of pine needles was clearly affected by habitat type and the planted tree species present, which implies that rapid habitat change impacts nest composition. We do not exclude that urbanization might impact other aspects of nest building behaviour not covered in our study (e.g. costs of searching for nest material), and that the strengths of the associations between urbanization and nest structures might differ among study populations or species.

Population Structure of the Raccoon Dog on the Grounds of the Imperial Palace, Tokyo, Revealed by Microsatellite Analysis of Fecal DNA.

The raccoon dog (Nyctereutes procyonoides, Canidae, Carnivora) is highly adaptable to urban environments. Populations of carnivorans inhabiting urban areas sometimes differ ecologically and genetically from those in rural areas. However, there is little information on urban raccoon dogs. This study focused on raccoon dog populations in Tokyo, one of the most highly urbanized cities in the world. We examined the genotypes of 10 microsatellites for 101 fecal samples from raccoon dogs inhabiting the grounds of the Imperial Palace, a green space in central Tokyo. We successfully genotyped 58 samples originating from 31 individuals. We also analyzed muscle tissue samples from raccoon dogs from the grounds of the Imperial Palace, the Akasaka Imperial Grounds (a green space close to the Imperial Palace), and the surrounding urban area, and then investigated the genetic structure and diversity of these populations, and the genetic differentiation among them. The population on the grounds of the Imperial Palace was genetically differentiated from that in the Akasaka Imperial Grounds, suggesting that the roads and buildings act as barriers to gene flow. In addition, the population on the grounds of the Imperial Palace showed greater genetic difference from that in the surrounding area than that in the Akasaka Imperial Grounds. We speculate that the moats around the Imperial Palace restrict individual ranges within the palace grounds and limit migration and gene flow to other areas.

Relative density of host-seeking ticks in different habitat types of south-western Slovakia.

Ixodes ricinus is a vector of microbial pathogens of medical and veterinary importance in Europe. Recently, increasing abundance of ticks has been observed in urban and suburban areas. The aim of this study was to investigate the tick species composition and examine correlations between local environmental variables and the relative density of host-seeking I. ricinus in two habitat types. Questing ticks were collected along six 100 m(2) transects in urban/suburban locations of Bratislava town, and in a non-fragmented deciduous forest in the Small Carpathians Mountains (south-western Slovakia) during 2011-2013. In total, 6015 I. ricinus were collected (3435 and 2580 in the urban/suburban and natural habitat, respectively), out of which over 80 % were nymphs. Haemaphysalis concinna comprised 1.3 % of the tick collections. Peak I. ricinus nymph and adult host-seeking activities were registered in April-June. Spatial and temporal variation in tick relative density and differences in the subadult/adult ratio were observed between habitats and between locations within the same habitat type. The relative density of questing I. ricinus nymphs correlated negatively with altitude, geographical aspect and saturation deficit in a 64-day period comprising the 8-day period including the date of tick sampling and previous 56 days. No significant correlation was found between roe deer density and questing nymph density. The study revealed the presence of abundant I. ricinus populations in green areas of Bratislava, suggesting a risk of exposure of town dwellers and domestic and companion animals to potentially infected ticks.

High Resolution Spatial Analysis of Habitat Preference of Aedes Albopictus (Diptera: Culicidae) in an Urban Environment.

Over the past decades, the Asian tiger mosquito (Aedes albopictus (Skuse, 1895)) has emerged in many countries, and it has colonized new environments, including urban areas. The species is a nuisance and a potential vector of several human pathogens, and a better understanding of the habitat preferences of the species is needed for help in successful prevention and control. So far, the habitat preference in urban environments has not been studied in Southern European cities. In this paper, spatial statistical models were used to evaluate the relationship between egg abundances and land cover types on the campus of Sapienza University in Rome, which is taken as an example of a European urban habitat. Predictor variables included land cover types, classified in detail on a high resolution image, as well as solar radiation and month of capture. The models account for repeated measures in the same trap and are adjusted for meteorological circumstances. Vegetation and solar radiation were found to be positively related to the number of eggs. More specifically, trees were positively related to the number of eggs and the relationship with grass was negative. These findings are consistent with the species' known preference for shaded areas. The unexpected positive relationship with solar radiation is amply discussed in the paper. This study represents a first step toward a better understanding of the spatial distribution of Ae. albopictus in urban environments.

Ecological drivers of nesting behavior in a subtropical city: An observational study on spotted doves.

Due to rapid homogenization in habitat types as a result of urbanization, some urban birds adapt their nesting strategies to changes in local habitat characteristics. Bird nesting decisions might have been mainly linked to resource constraints and ensuring reproductive success. In this study, we examined patterns of nesting behavior by spotted doves (Spilopelia chinensis) in a rapidly urbanizing area of Nanchang, China using ArcGIS 10.8, satellite tracking, camera traps, and field survey. To explore the mechanisms underlying nesting behavior in urban habitats, we assessed the correlations between nest reuse and reproductive success, and between nest reuse and nest predation. From December 2018 to December 2021, a total of 302 breeding nests were surveyed. The results revealed that the nest reuse rate was 38.08% (n = 115). Nests closer to trunk, with lower nest position and higher large-scale urbanization score tended to have higher reuse rate. In addition, nests with the higher the nest height and percent of canopy cover, and the lower small-scale urbanization score were more likely to reproduce successfully, and the reused nests also reproduce more successfully. The reproductive success associated with nest reuse was significantly higher than that associated with new nests (χ 2 = 8.461, p = .004). High degree of urbanization promoted nest reuse of spotted doves (large-scale urbanization score, z = 2.094, p = .036), which apparently enhanced their reproductive success (nest reuse, z = 2.737, p = .006). In conclusion, a nest structure with good permeability is the material basis for the nest reuse in spotted dove, while the relatively low risk of predation in urban habitat and the scarcity of nest site resources due to urbanization increase the tendency of birds to reuse old nests, which is associated with their reproductive success and evolutionary fitness.

Microbial response to Nature-Based Solutions in urban soils: A comprehensive analysis using Biolog® EcoPlates™.

The study presents a comprehensive examination of changes in soil microbial functional diversity (hereafter called microbial activity) following the implementation of Nature-Based Solutions (NBS) in urban areas. Utilizing the Biolog® EcoPlates™ technique, the study explored variations in microbial diversity in urban soil under NBSs implementation across timespan of two years. Significant differences in microbial activity were observed between control location and those with NBS implementations, with seasonal variations playing a crucial role. NBS positively impacted soil microbial activity especially at two locations: infiltration basin and wild flower meadow showing the most substantial increase after NBS implementation. The study links rainfall levels to microbial functional diversity, highlighting the influence of climatic conditions on soil microbiome. The research investigates also the utilization of different carbon sources by soil microorganisms, shedding light on the specificity of substrate utilization across seasons and locations. The results demonstrate that NBSs implementations lead to changes in substrate utilization patterns, emphasizing the positive influence of NBS on soil microbial communities. Likewise, biodiversity indices, such as Shannon-Weaver diversity (H'), Shannon Evenness Index (E), and substrate richness index (S), exhibit significant variations in response to NBS. Notably, NBS implementation positively impacted H' and E indexes, especially in infiltration basin and wild flower meadow, underlining the benefits of NBS for enhancing microbial diversity. The obtained results demonstrated valuable insight into the dynamic interactions between NBS implementation and soil microbial activity. The findings underscore the potential of NBS to positively influence soil microbial diversity in urban environments, contributing to urban sustainability and soil health. The study emphasizes the importance of monitoring soil microbial activity to assess the effectiveness of NBS interventions and guides sustainable urban development practices.

Physiological phenotypes differ among color morphs in introduced common wall lizards (Podarcis muralis).

Many species exhibit color polymorphisms which have distinct physiological and behavioral characteristics. However, the consistency of morph trait covariation patterns across species, time, and ecological contexts remains unclear. This trait covariation is especially relevant in the context of invasion biology and urban adaptation. Specifically, physiological traits pertaining to energy maintenance are crucial to fitness, given their immediate ties to individual reproduction, growth, and population establishment. We investigated the physiological traits of Podarcis muralis, a versatile color polymorphic species that thrives in urban environments (including invasive populations in Ohio, USA). We measured five physiological traits (plasma corticosterone and triglycerides, hematocrit, body condition, and field body temperature), which compose an integrated multivariate phenotype. We then tested variation among co-occurring color morphs in the context of establishment in an urban environment. We found that the traits describing physiological status and strategy shifted across the active season in a morph-dependent manner-the white and yellow morphs exhibited clearly different multivariate physiological phenotypes, characterized primarily by differences in plasma corticosterone. This suggests that morphs have different strategies in physiological regulation, the flexibility of which is crucial to urban adaptation. The white-yellow morph exhibited an intermediate phenotype, suggesting an intermediary energy maintenance strategy. Orange morphs also exhibited distinct phenotypes, but the low prevalence of this morph in our study populations precludes clear interpretation. Our work provides insight into how differences among stable polymorphisms exist across axes of the phenotype and how this variation may aid in establishment within novel environments.

Telemetry and Accelerometer Tracking of Green Toads in an Urban Habitat: Methodological Notes and Preliminary Findings.

Advancements in tracking technologies provide an increasingly important tool in animal monitoring and conservation that can describe animal spatial behavior in native habitats and uncover migratory routes that otherwise may be difficult or impossible to map. In addition, high-resolution accelerometer sensors provide powerful insights into animal activity patterns and can help to identify specific behaviors from accelerometer profiles alone. Previously, such accelerometers were restricted to larger animals due to size and mass constraints. However, recent advances make it possible to use such devices on smaller animals such as the European green toad (Bufotes viridis), the focus of our current study. We deploy custom made tracking devices, that consist of very-high-frequency transmitters and tri-axial accelerometers, to track toads in their native urban environment in Vienna (Austria). A total of nine toads were tracked, ranging from three to nine tracking days per individual during the post-breeding season period. We demonstrate that our devices could reliably monitor toad movement and activity during the observation period. Hence, we confirmed the predominantly nocturnal activity patterns and recorded low overall movement at this urban site. Accelerometer data revealed that toads exhibited brief but intense activity bursts between 10 pm and midnight, resting periods during the night and intermittent activity during the day. Positional tracking alone would have missed the major activity events as they rarely resulted in large positional displacements. This underscores the importance of and value in integrating multiple tracking sensors for studies of movement ecology. Our approach could be adapted for other amphibians or other animals with mass constraints and may become standard monitoring equipment in the near future.

Assessment of the ecological role of historic centres based on the relationship between biodiversity and urban composition.

Historic centres provide their inhabitants with identity and well-being. Although studies focus on the conservation of the built environment, in recent years the environmental component of historic centres has also been analysed. Issues such as ecosystem services and biodiversity have become more pressing due to rapid population growth, development and the climate crisis. Green spaces in historic centres often conserve biodiversity, as they tend to be protected spaces. This article presents two case studies in Castellón de la Plana (Spain) with the aim of analysing the relationship between the built environment and avian biodiversity. The study uses a four-phase process. The first phase incorporates a review of recent literature to select biodiversity indicators, while the second focuses on open data analysis and incorporation into GIS software. The third phase consists of field data collection and the mapping of biodiversity indicators. Finally, phase four involves the preparation of thematic maps, which allows us to visualize behavioural patterns connecting bird colonies with the morphology of the built environment in order to draw relevant conclusions that can help improve biodiversity. The analysis allowed the calculation of eight indicators and the identification of building typologies, the percentage of green areas and the inspection of features promoting avian biodiversity. In total, 31 bird species were detected, 27 in the historic centre of Castellón and 26 in the historic centre of the Grao district. Among them, the mapping distribution of three endangered species demonstrates their dependence on these historic built habitats. Apus apus, Passer domesticus and Delichon urbicum are present in 97, 82 and 56% of grids, respectively, with ANOVA correlation confirming these species densities found. The study is somewhat limited in the use of the line transect method due to the potential structural biases intrinsic to the unique nature of the districts assessed.

Study on the coupling and coordination degree between urban tourism development and habitat environment in the Yangtze River Delta in China.

By constructing the two evaluation systems of urban tourism development (TD) and habitat environment (HE), the dynamic response relationship between the two systems in the Yangtze River Delta urban agglomeration from 2001 to 2020 is explored by using panel vector autoregression (PVAR) model and coupled coordination degree model. The study unearthed four intriguing findings: (1) the level of TD in the study area has been continuously rising from 2001 to 2020, with an initial slow growth rate and then fast. The level of HE is increasing steadily at an average annual rate of 7.05%. There exists a reciprocal response relationship between the two systems, with a strong shock effect in the short term and a synergistic evolution in the long term. (2) The coupling degree between the urban TD and HE has an increasing trend, and the coupling coordination degree of the two systems has an average annual rate of 4.165%, implying the interaction and promotion effect between the two systems is improving. (3) Most of the cities in the Yangtze River Delta gradually realize the evolution from dysfunctional type to coordinated type, but the overall coordination intensity is low. (4) The barrier degrees of TD system indicators show a small annual increasing trend, while the barrier degrees of HE system indicators show a substantial and continuous decreasing trend. In terms of the barrier degree factors, some important factors that restrict the coupled and coordinated development of the two systems are also reported. This research can provide a useful reference for the synergistic improvement of urban tourism economy and habitat environment.

Lockdown effects on fear revealed direct and indirect effects of human presence on perceived predation risk.

The Covid-19 lockdown reduced drastically human presence outdoors, providing an uncontrolled experiment for disentangling direct and indirect effects of human presence on animal fearfulness. We measured 18,494 flight initiation distances (FIDs, the distance at which individual animals fly away when approached by a human) from 1333 populations of 202 bird species taken in four European cities both before, during and after the lockdown. FIDs decreased during lockdown in rural habitats but increased in urban habitats, especially for singing birds. Height above ground increases during lockdown in non-singing birds only, and birds adjusted horizontal tolerance to approach according to height outside lockdown, in rural habitats and while not singing. Responses showed lagged effects after lockdown in urban but not in rural habitats. Differential responses to lockdown among habitats and between signing and non-singing birds were consistent with relaxation of direct disturbance effects on birds in rural habitats during lockdown, as well as with increased indirect fear effects mediated by predator release in cities. FIDs seemed to measure the balance of direct and indirect effects of humans on predations risk and food needs rather than direct effects of humans on fear.

MOVEMENTS BY SAN JOAQUIN KIT FOXES (VULPES MACROTIS MUTICA) BETWEEN URBAN AND NONURBAN HABITATS: IMPLICATIONS FOR INTERPOPULATION DISEASE TRANSFER.

Sarcoptic mange epidemics erupted in two of the remaining populations of endangered San Joaquin kit foxes (Vulpes macrotis mutica). Both populations are in urban habitats in the cities of Bakersfield and Taft, California, USA. The risk of disease spread from the two urban populations to nearby nonurban populations, and then throughout the species range, is of considerable conservation concern. To date, mange has not been detected in any nonurban populations despite considerable surveillance effort. The reasons for the lack of detections of mange among nonurban foxes are unknown. We monitored urban kit fox movements using geographic positioning system (GPS) collars to test the hypothesis that urban foxes were not venturing into nonurban habitats. Of 24 foxes monitored December 2018 to November 2019, 19 (79%) made excursions from urban into nonurban habitats from 1-124 times. The mean number of excursions per 30 d was 5.5 (range 0.1-13.9 d). The mean proportion of locations in nonurban habitats was 29.0% (range 0.6-99.7%). The mean maximum distance that foxes traveled into nonurban areas from the urban-nonurban interface was 1.1 km (range 0.1-2.9 km). Mean number of excursions, proportion of nonurban locations, and maximum distance into nonurban habitats were similar between Bakersfield and Taft, females and males, and adults and juveniles. At least eight foxes apparently used dens in nonurban habitats; shared use of dens may be an important mode of mange mite transmission between conspecifics. Two of the collared foxes died of mange during the study and two others had mange when captured at the end of the study. Three of these four foxes had made excursions into nonurban habitats. These results confirm a significant potential for mange to spread from urban to nonurban kit fox populations. We recommend continued surveillance in nonurban populations and continued treatment efforts in the affected urban populations.

Natural versus urban global soil organic carbon stocks: A meta-analysis.

Increasingly, the human existence in urban environments is growing. In addition, anthropogenic activity has altered the global carbon (C) cycle and triggered climate change. Soil is the largest pool of organic C in terrestrial ecosystems, but its ability to retain and store C varies. As humans move forward to mitigate climate change, there is a growing need to understand the C storing capacity of soils and their interactions with factors like climate, vegetation or a footprint of human activity. Here, we constructed a meta-analysis which focused on 30 cm soil depth by collecting data from over 191 studies measuring soil organic carbon (SOC) stocks across natural, urban green space, and urban intensive habitats. We then compared the SOC data between different climatic zones, vegetation types, and anthropogenic influences with the human footprint index. The results indicate that SOC stocks in natural habitats (98.22 ± 49.10 Mg ha-1) are significantly higher than those of urban green spaces (54.61 ± 22.02 Mg ha-1) and urban intensive habitats (65.88 ± 35.27 Mg ha-1). We find a significant and negative relationship between the human footprint and SOC stocks of natural habitats but not between the human footprint and either of the urban habitats. Urban intensive and urban green space habitat soils store less C than natural ones. However, when compared across climatic zones or vegetation types, the capacity of natural soils to store C is variable and vulnerable to human activity. Carbon storage in urban soils is likely limited by persistent and stable anthropogenic influences keeping variability low. This is most pronounced in urban green spaces where human management is high (i.e. a golf course) and SOC is low. A comprehensive understanding of C storage in soils is essential to land management and climate mitigation measures.

Comparing Satellite and Ground-Based Measurements of Environmental Suitability for Vector Mosquitoes in an Urban Landscape.

Exposure to mosquito-borne diseases is influenced by landscape patterns and microclimates associated with land cover. These influences can be particularly strong in heterogeneous urban landscapes where human populations are concentrated. We investigated how land cover and climate influenced abundances of Ae. albopictus (Skuse) (Diptera: Culicidae) and Cx. quinquefasciatus (Say) (Diptera: Culicidae) in Norman, Oklahoma (United States). From June-October 2019 and May-October 2020 we sampled mosquitoes along an urban-rural gradient using CO2 baited BG Sentinel traps. Microclimate sensors at these sites measured temperature and humidity. We mapped environmental variables using satellite images from Landsat, Sentinel-2, and VIIRS, and the CHIRPS rainfall dataset. We also obtained meteorological data from the closest weather station. We compared statistical models of mosquito abundance based on microclimate, satellite, weather station, and land cover data. Mosquitoes were more abundant on trap days with higher temperature and relative humidity. Rainfall 2 wk prior to the trap day negatively affected mosquito abundances. Impervious surface cover was positively associated with Cx. quinquefasciatus and tree cover was negatively associated with Ae. albopictus. Among the data sources, models based on satellite variables and land cover data had the best fits for Ae. albopictus (R2 = 0.7) and Cx. quinquefasciatus (R2 = 0.51). Models based on weather station or microclimate data had weaker fits (R2 between 0.09 and 0.17) but were improved by adding land cover variables (R2 between 0.44 and 0.61). These results demonstrate the potential for using satellite remote sensing for mosquito habitat analyses in urban areas.

Plasticity, exudation and microbiome-association of the root system of Pellitory-of-the-wall plants grown in environments impaired in iron availability.

The investigation of the adaptive strategies of wild plant species to extreme environments is a challenging issue, which favors the identification of new traits for plant resilience. We investigated different traits which characterize the root-soil interaction of Parietaria judaica, a wild plant species commonly known as "Pellitory-of-the-wall". P. judaica adopts the acidification-reduction strategy (Strategy I) for iron (Fe) acquisition from soil, and it can complete its life cycle in highly calcareous environments without any symptoms of chlorosis. In a field-to-lab approach, the microbiome associated with P. judaica roots was analyzed in spontaneous plants harvested from an urban environment consisting in an extremely calcareous habitat. Also, the phenolics and carboxylates content and root plasticity and exudation were analyzed in P. judaica plants grown under three different controlled conditions mimicking the effect of calcareous environments on Fe availability: results show that P. judaica differentially modulates root plasticity under different Fe availability-impaired conditions, and that it induces, to a high extent, the exudation of caffeoylquinic acid derivatives under calcareous conditions, positively impacting Fe solubility.