Effect of land use and soil depth on the distribution of phyto-available nutrients and SOC pools of Vertisols in Central India.

A proper understanding of soil parameters under different production systems of the world is necessary for efficient soil management. We, therefore, carried out the present study to assess the status of some selected soil properties (soil pH and electrical conductivity (EC)), phyto-available nutrients (available nitrogen (AN), available potassium (AK), exchangeable calcium (Ex. Ca), exchangeable magnesium (Ex. Mg), available sulfur (AS), and soil organic carbon (SOC) pools (SOC, total organic carbon (TOC), very labile C, labile C, less labile C, and non-labile C) and to establish relationships among the measured soil parameters at different depths of Vertisols of India under various land uses. A total of 150 composite soil samples (from 25 plots including nine from agricultural land, nine from horticultural land, three from forest land, and four from grassland) were collected from 6 soil depths viz, 0-10, 10-20, 20-40, 40-60, 60-80, and 80-100 cm under agriculture, horticulture, forest, and grassland land uses present in Central India and analyzed. The values of soil pH, EC, AN, AK, Ex. Ca, Ex. Mg, and AS in various soil depths under different land uses varied widely. The values of SOC (0.19 to 1.00%), TOC (0.58 to 2.42%), very labile C (0.14 to 0.83%), labile C (0.05 to 0.25%), less labile C (0.05 to 0.26%) and non-labile C (0.23 to 1.42%) in various soil depths under different land uses also varied significantly. Forest and grassland land uses had higher levels of SOC, TOC, very labile, and non-labile C content in all the soil depths in comparison to SOC, TOC, very labile, and non-labile C content in different soil depths under agriculture and horticulture land use. The levels of SOC, TOC, very labile, and non-labile C content under all the land uses decreased with increasing soil depths. SOC was positively and significantly correlated with AN, AK, AS, and estimated SOC pools in surface soil layers. Principal component analysis (PCA) of soil parameters in different soil depths resulted in 5 principal components (PCs) with > 1 eigenvalue and accounting for > 75% variability. This information could be used for managing SOC status and phyto-available nutrients in Vertisols under different land uses.

Agronomic iodine biofortification of leafy vegetables grown in Vertisols, Oxisols and Alfisols.

Iodine deficiency disorders (IDD) in sub-Saharan African countries are related to low dietary I intake and generally combatted through salt iodisation. Agronomic biofortification of food crops may be an alternative approach. This study assessed the effectiveness of I biofortification of green vegetables (Brassica napus L and Amaranthus retroflexus L.) grown in tropical soils with contrasting chemistry and fertility. Application rates of 0, 5 and 10 kg ha-1 I applied to foliage or soil were assessed. Leaves were harvested fortnightly for ~ 2 months after I application before a second crop was grown to assess the availability of residual soil I. A separate experiment was used to investigate storage of I within the plants. Iodine concentration and uptake in sequential harvests showed a sharp drop within 28 days of I application in all soil types for all I application levels and methods. This rapid decline likely reflects I fixation in the soil. Iodine biofortification increased I uptake and concentration in the vegetables to a level useful for increasing dietary I intake and could be a feasible way to reduce IDD in tropical regions. However, biofortification of green vegetables which are subject to multiple harvests requires repeated I applications.

Pollution assessment of potentially toxic elements in soils of different taxonomy orders in central Greece.

Four hundred fifty soil samples of the orders of Alfisols, Inceptisols, Endisols, and Vertisols from Karditsa, Trikala, and Larissa (Central Greece) were collected over a three-year period. In these samples we analyzed potentially toxic elements (PTEs) and soil properties known to affect their mobility. High regression coefficients were observed between soil pH and PTE concentrations in Alfisols, reflecting that soil pH is the dominant characteristic influencing PTEs. In Inceptisols, there was a significant interaction among the studied PTEs, probably due to PTEs having the same origin. The Endisol samples had high sand content and electrical conductivity values, resulting in high availability of all studied PTEs. In Vertisols, clay content proved to be the most important parameter influencing PTE levels. Factor analysis was also used in order to clarify the possible sources of metals in the studied areas.

Budgeting of major nutrients and the mitigation options for nutrient mining in semi-arid tropical agro-ecosystem of Tamil Nadu, India using NUTMON model.

Mining of nutrients from soil is a major problem in developing countries causing soil degradation and threaten long-term food production. The present study attempts to apply NUTrient MONitoring (NUTMON) model for carrying out nutrient budgeting to assess the stocks and flows of nitrogen (N), phosphorus (P), and potassium (K) in defined geographical unit based on the inputs, viz., mineral fertilizers, manures, atmospheric deposition, and sedimentation, and outputs, viz., harvested crop produces, residues, leaching, denitrification, and erosion losses. The study area covers Coimbatore and Erode Districts, which are potential agricultural areas in western agro-ecological zone of Tamil Nadu, India. The calculated nutrient balances for both the districts at district scale, using NUTMON methodology, were negative for nitrogen (N -3.3 and -10.1 kg ha(-1)) and potassium (K -58.6 and -9.8 kg ha(-1)) and positive for phosphorus (P +14.5 and 20.5 kg ha(-1)). Soil nutrient pool has to adjust the negative balance of N and K; there will be an expected mining of nutrient from the soil reserve. A strategy was attempted for deriving the fertilizer recommendation using Decision Support System for Integrated Fertilizer Recommendation (DSSIFER) to offset the mining in selected farms. The results showed that when DSSIFER recommended fertilizers are applied to crops, the nutrient balance was positive. NUTMON-Toolbox with DSSIFER would serve the purpose on enhancing soil fertility, productivity, and sustainability. The management options to mitigate nutrient mining with an integrated system approach are also discussed.

Precursor crop and vertisols type influences on teff (Eragrostis tef) response to fertilizer rates in the central highlands of Ethiopia.

Optimization of fertilizer-N and -P is important to highland production of teff (Eragrostis tef) on Vertisols of central Ethiopia but may be affected by precursor crop and Vertisols type. On-farm experiments were conducted in three major teff growing districts of North Shewa (Moretina jiru, Ensaro, and Merhabete) in 2019 and 2020 with the main objectives was to determine the effect of precursor crops and Vertisols type on teff response to N and P rates. The N x P factorial combinations include 0, 60, 120, 180, and 240 kg N ha-1 and 0, 30, 60, and 90 kg P ha-1 applied each to light and heavy Vertisols with either cereal or pulse precursor crops in each district. In Merhabete, grain yield was significantly influenced by Pc x Vt x N and Pc x Vt x P but always with the lowest and highest grain yield with 0 and 240 kg N ha-1, respectively. Yield was 394 % more with 240 kg N ha-1 compared with no N and P applied. The Vt x Pc × N interaction affected teff yield in Moretina Jiru as application of 240 kg N ha-1 increased teff yield by 440 %, 30 %, 23 %, and 7 % on light Vertisols compared with 200 %, 16 %, 13 %, and 2 % on heavy Vertisols. The 4-way interaction of Vt x Pc x N x P affected grain yield in Ensaro due to the low N and P status of the soil coupled with the distinct Vertisols type in the district. In all districts, yield response to N was greater with pulse compared with cereal precursor crops and with a greater response for heavy compared with light Vertisols in Moretina Jiru and Ensaro. In Moretina Jiru, application of 170 kg-1N and soil maintenance level of 30 kg-1 of P ha-1 are recommended as an economic optimum rate (EOR). In Ensaro, the EOR for teff following cereal on light Vertisols are 166 kg N ha-1 and 65 kg P ha-1. At Ensaro, needed rates for teff following pulse on light Vertisols are 198 N ha-1 and 48 kg P ha-1. At Ensaro, needed rates for teff following cereal on heavy Vertisols are 240 N ha-1 and 90 kg P ha-1. At Ensaro, needed rates for teff following pulse on heavy Vertisols are 240 N ha-1 and 80 kg P ha-1. In Merhabete, the EOR for teff following cereal on light Vertisols are 182 kg N ha-1 and 60 kg P ha-1. In Merhabete, needed rates for teff following pulse on light Vertisols are 206 N ha-1 and 64 kg P ha-1. In Merhabete, needed rates for teff following cereal on heavy Vertisols are 240 N ha-1 and 90 kg P ha-1. In Merhabete, needed rates for teff following pulse on heavy Vertisols are 218 N ha-1 and 58 kg P ha-1. Therefore, those N and P rate are recommended for the study area, soil type and precursor crops.

Evaluating different green manuring plant species and stool destruction methods for enhancing sugarcane yield at Wonji-Shoa Sugar Estate, Ethiopia.

The long-term intensive production system employed in the Sugar Estates in Ethiopia, characterized by monoculture, preharvest burning, and excessive tillage, has led to soil degradation with a concomitant decline in sugarcane yield. Therefore, a study was conducted at Wonji-Shoa Sugar Estate (WSSE) with the objective of evaluating the effectiveness of different green manuring plant species and cane stool destruction methods (SDMs) in improving cane yield. To that end, seven green manure plant species (sunn-hemp, lablab, cowpea, soybean, mungbean, dhaincha, and sugarcane trash) were evaluated under three SDMs (cultivating-out, spraying-out with herbicide, and maintaining the stool as it is) using a split-plot design. The experimental fields were established on two major soil types, with green manure crops incorporated into the soil before planting the sugarcane. The dry matter production and nitrogen contribution of the green manure plants, as well as the height, population, diameter, and yields of sugarcane, were determined and subsequently subjected to statistical and economic analysis. The results showed that cowpea, followed by lablab, dhaincha, and sunn hemp, were the most effective green manures in terms of improving cane performance, with up to 17-20 % yield advantage and 21-40 % net economic benefits over the control treatment. Additionally, the spraying-out MSD was as effective as the cultivating-out MSD, but both outperformed maintaining the stool. In conclusion, utilizing these green manuring crops in combination with the spraying-out SDM presents notable advantages for improved cane yield and enhanced economic benefits in a sustainable manner. Adoption of these practices, therefore, holds significant potential for reversing the constantly declining sugarcane yields at WSSE.

Characterizing soil hydrology in the Indo-Gangetic plain of Bihar, India: Methods and preliminary results.

In the Eastern Gangetic Plain (EGP) soil hydrology is a major determinant of land use and also governs the ecosystem services derived from cropping systems, particularly greenhouse gas (GHG) emissions from rice fields. To characterize patterns of soil hydrology in these, daily field monitoring of water levels was conducted during the monsoon (kharif) season in a comparatively wet (2021) and dry (2022) year with flooding depth and drainage tracked with field water tubes across 47 (2021) and 183 (2022) locations. Fields were clustered into hydrologic response types (HRT) which can then be used for land surface modelling, land use recommendations, and to target agronomic interventions that contribute to sustainable development outcomes. Clusters based on two methods of summarizing a single information source were compared. The information source was a time-series of field water-level observations, and the two methods were (1) the original time-series and their first differences and (2) a set of derived hydrologic descriptors that are conceptually related to greenhouse gas (GHG) emissions. Clustering was (1) by k-means with an optimization of cluster numbers and (2) by hierarchical clustering with the same number of clusters as identified by k-means. Hydrologic behaviour shifted dramatically between growing seasons, and it was not possible to identify consistent HRT's across years. The clusters had only a weak relation with soil properties, almost no relation with farmer perception of relative landscape position, and no relation with rice establishment method. Clusters based on time-series were moderately well predicted in the dry year 2022 by optimized random forest models, with the most important predictors being the number of irrigations, seasonal precipitation, pre-monsoon groundwater levels, seasonal groundwater level change, and pH, this latter as a surrogate for landscape position and other soil properties. In the wet year 2021 clusters were (poorly) predicted by just seasonal precipitation and pre-monsoon groundwater levels. This shows the complex relation of soil hydrology with landscape position and land management, as well as synoptic climate. By contrast, clusters based on the descriptors were not well-matched with those from the time-series, and could not be well predicted by random forest models. This shows that different clustering criteria may result in different interpretations of the landscape hydrology and thus different heuristics for anticipating the hydrology of a given field under different management choices.

Agronomic biofortification increases grain zinc concentration of maize grown under contrasting soil types in Malawi.

Zinc (Zn) deficiency remains a public health problem in Malawi, especially among poor and marginalized rural populations, linked with low dietary intake of Zn due to consumption of staple foods that are low in Zn content. The concentration of Zn in staple cereal grain can be increased through application of Zn-enriched fertilizers, a process called agronomic biofortification or agro-fortification. Field experiments were conducted at three Agricultural Research Station sites to assess the potential of agronomic biofortification to improve Zn concentration in maize grain in Malawi as described in registered report published previously. The hypotheses of the study were (i) that application of Zn-enriched fertilizers would increase in the concentration of Zn in maize grain to benefit dietary requirements of Zn and (ii) that Zn concentration in maize grain and the effectiveness of agronomic biofortification would be different between soil types. At each site two different subsites were used, each corresponding to one of two agriculturally important soil types of Malawi, Lixisols and Vertisols. Within each subsite, three Zn fertilizer rates (1, 30, and 90 kg ha-1) were applied to experimental plots, using standard soil application methods, in a randomized complete block design. The experiment had 10 replicates at each of the three sites as informed by a power analysis from a pilot study, published in the registered report for this experiment, designed to detect a 10% increase in grain Zn concentration at 90 kg ha-1, relative to the concentration at 1 kg ha-1. At harvest, maize grain yield and Zn concentration in grain were measured, and Zn uptake by maize grain and Zn harvest index were calculated. At 30 kg ha-1, Zn fertilizer increased maize grain yields by 11% compared with nationally recommended application rate of 1 kg ha-1. Grain Zn concentration increased by 15% and uptake by 23% at the application rate of 30 kg ha-1 relative to the national recommendation rate. The effects of Zn fertilizer application rate on the response variables were not dependent on soil type. The current study demonstrates the importance of increasing the national recommendation rate of Zn fertilizer to improve maize yield and increase the Zn nutritional value of the staple crop.

Soil parameters affecting the levels of potentially harmful metals in Thessaly area, Greece: a robust quadratic regression approach of soil pollution prediction.

The behavior and possible contamination risk due to the presence of potentially harmful metals (PHM) were studied based on 2250 soil samples that were collected in a 5-year period (2013-2017) from the plain of Thessaly (prefectures of Karditsa, Trikala, and Larissa). The vertical distribution of metals was also investigated from sample profiles at three depths 0-30, 30-60, and 60-90cm. The soils of the sampling belong to four taxonomy soil orders that are dominant in the studied area (Alfisols, Inceptisols, Endisols, and Vertisols). In a novel approach, robust quadratic regression analysis on multiple variables was used to define prediction models of the concentrations of two metals: Fe which is an essential metal and the toxic Cd. Linear and quadratic regression formulae were estimated based on the iteratively reweighted least squares robust regression approach in an effort to eliminate the impact of the outliers. These formulae define how several soil properties affect the distribution of the considered metals in each soil order. The evaluation of the estimated regression equations based on the R2 metric indicates that they constitute a useful, reliable, and valuable tool for managing, describing, and predicting the pollution in the studied area.

Vertical distribution of soil seed bank and the ecological importance of deeply buried seeds in alkaline grasslands.

Background: Soil seed banks play a central role in vegetation dynamics and may be an important source of ecological restoration. However, the vast majority of seed bank studies examined only the uppermost soil layers (0-10 cm); hence, our knowledge on the depth distribution of seed bank and the ecological significance of deeply buried seeds is limited. The aim of our study was to examine the fine-scale vertical distribution of soil seed bank to a depth of 80 cm, which is one of the largest studied depth gradients so far. Our model systems were alkaline grasslands in East-Hungary, characterised by harsh environmental conditions, due to Solonetz soil reference group with Vertic horizon. We asked the following questions: (1) How do the seedling density and species richness of soil seed bank change along a vertical gradient and to what depth can germinable seeds be detected? (2) What is the relationship between the depth distribution of the germinable seeds and the species traits? Methods: In each of the five study sites, four soil cores (4 cm diameter) of 80 cm depth were collected with an auger for soil seed bank analysis. Each sample was divided into sixteen 5-cm segments by depth (320 segments in total). Samples were concentrated by washing over sieves and then germinated in an unheated greenhouse. Soil penetration resistance was measured in situ next to each core location (0-80 cm depth, 1-cm resolution). We tested the number and species richness of seedlings observed in the soil segments (N = 320), using negative binomial generalized linear regression models, in which sampling layer and penetration resistance were the predictor variables. We ran the models for morphological groups (graminoids/forbs), ecological groups (grassland species/weeds) and life-form categories (short-lived/perennial). We also tested whether seed shape index, seed mass, water requirement or salt tolerance of the species influence the vertical distribution of their seed bank. Results: Germinable seed density and species richness in the seed bank decreased with increasing soil depth and penetration resistance. However, we detected nine germinable seeds of six species even in the deepest soil layer. Forbs, grassland species and short-lived species occurred in large abundance in deep layers, from where graminoids, weeds and perennial species were missing. Round-shaped seeds were more abundant in deeper soil layers compared to elongated ones, but seed mass and ecological indicator values did not influence the vertical seed bank distribution. Our research draws attention to the potential ecological importance of the deeply buried seeds that may be a source of recovery after severe disturbance. As Vertisols cover 335 million hectares worldwide, these findings can be relevant for many regions and ecosystems globally. We highlight the need for similar studies in other soil and habitat types to test whether the presence of deep buried seeds is specific to soils with Vertic characteristics.

Effects of phosphorus and sulfur on yield and nutrient uptake of wheat (Triticum aestivum L.) on Vertisols, North Central, Ethiopia.

Deficiency of phosphorus (P) and sulfur (S) is increasingly being reported in soils of Ethiopia. While some studies have shown significant response of wheat to P and S application, information on the response of wheat to P and S application interactively is conspicuously lacking. In this regard, we evaluated the response of wheat to P and S application interactively in the study area. A field experiment was conducted at two locations to determine the effects of P and S, on yield, uptake and P, S use efficiency of bread wheat. A factorial combination of four levels of P (0,11,22 and 44 kg h-1) and three levels S (0, 15 and 30 kg ha-1) laid out in Randomized Complete Block Design with three replications. Results revealed that interacted application of P and S at 22 and 15 kg ha-1 respectively increased grain yield of wheat by 40.1 % over control. The corresponding increase with straw was 53.4 % over control. Wheat yield obtained with combined application of P and S greater than single application of P or S indicating synergistic between them. The maximum grain N (56.3 kg ha-1), P (12.8 kg ha-1) and S (4.2 kg ha-1) uptakes were obtained due to combined application of P and S at 22 P and 15 S kg ha-1. Agronomic efficiencies of P and S decreased as the rates of P and S application increased. Combined fertilization of S and P is necessary in the study district and 15 kg S combined with 22 kg P ha-1 produced the highest yield. Thus, this treatment is found to be recommended for bread wheat production in Vertisols of the district. While, partial budget analysis result revealed that, combination of 22P and 15S kg ha-1 produced the highest MMR (54.9 %) and thus, this treatment is found to be economically feasible treatment for bread wheat production in study area of the district. We recommend further experiments on different combination of P with S in different agro-ecologies and soil types are required for confirmation of results and the residual effect of P and S on the following crop is needed to study the long-term effect of P and S.

The Effect of Increasing Topsoil Disturbance on Surface-Active Invertebrate Composition and Abundance under Grazing and Cropping Regimes on Vertisols in North-West New South Wales, Australia.

Agricultural intensification practices involve varying degrees of disturbance to the soil ecosystem. This study evaluated six agricultural management regimes with increasing levels of topsoil disturbance, on the composition and abundance of surface-active invertebrates on Vertisols at a sub-catchment scale. Two grazing (native and introduced pastures), and four cropping (combining short and long fallow, with zero and conventional tillage) management regimes were examined. Surface-active invertebrates were collected seasonally with pitfall traps over 2 years (8 seasons), and identified to order, while ants (Formicidae) that comprised 47% of total invertebrates collected, were identified to genera. Season had a significant effect on ant abundance and number of genera recorded with higher abundance and twice the number of genera in summer than all other seasons. Ants, particularly Iridomyrmex, were mainly active in summer, while other invertebrates especially Coleoptera, were more active in winter. Surface-active invertebrates were 30% more abundant in grazing than cropping land use types. Native pasture, with little surface soil disturbance, recorded the highest number of invertebrates, mainly ants, compared to other agricultural management regimes. Coleoptera and Dermaptera were higher in abundance under conventional tillage compared with those agricultural management regimes that disturb the topsoil less. Optimizing surface-active invertebrate activity on Vertisols for most taxa will require reducing topsoil disturbance. However, the research findings also suggest that the impact of agricultural management regimes on invertebrate activity was difficult to predict with any certainty as the three main ant genera, and most abundant invertebrate collected, did not respond in a consistent manner.

Dataset on the Mediterranean soils from the coastal region of the Lattakia governorate, Syria.

Soil survey is indispensable for land-use planning in any agro-ecosystem, particularly in coastal ecosystems because they often face several environmental problems such as flooding and water pollution, leading to soil degradation. The data given in this article revealing the common soil types and substantial taxonomy levels in the coastal region of Lattakia, Syria which is a key question for the land-use planning in the region. Data from 30 representative soil profiles and 60 auger points covering different agroecosystems within the Mediterranean coastal region of the Lattakia governorate, Syria were studied. The database including, the field morphological characteristics, physicochemical, mineralogical and micromorphological laboratory analyses. Entisols, Inceptisols, Mollisols, and Vertisols are the main soil types demonstrated in the area, which requiring convenient management for these divergent soils. The full profile data is available online in this data article for further reuse and for appropriate decisions to manage these soils.

Response of crops to fertilizer application in volcanic soils.

Potassium (K) has been considered as a non-deficient nutrient in most Ethiopian soils. However, some studies recommended K application to K sufficient soils if K/Mg ratio is < 0.7. To resolve this controversy, field experiments and laboratory soil analysis were conducted in two districts (Yilmana Densa and Dera) in north-western Ethiopia on Mollic Nitisols (aric, humic) (pH = 5.5), Pellic Vertisols (aric, gilgaic, mazic) (pH = 6.2) and Vertic Luvisols (aric, nitic) (pH = 5.2) using wheat, tef and maize, respectively as test crops. The field experiments were laid out in a randomized complete block design with 7 K fertilizer rates (0, 42, 83, 125, 166, 208, and 249 kg ha-1 K) and four replications. KCl and DAP (200 kg ha-1) fertilizers were added at planting. Urea (200 kg ha-1) was added in split, half at planting and half at tillering for wheat and tef; and at knee-height stage for maize. Soil samples were taken two weeks after planting to determine K and Mg contents, K critical levels and optimum K/Mg ratio. The ammonium acetate extraction method was used to determine the K and Mg contents in the soil using a flame photometer and an atomic absorption spectrometer, respectively. Yield data were collected after harvest. The results of the study indicated that Mollic Nitisols (aric, humic), Pellic Vertisols (aric, gilgaic, mazic) and Vertic Luvisols (aric, nitic) had high K contents of 351, 380 and 434 mg kg-1, respectively. Rising K fertilizer levels increased soil K contents; however, this did not significantly increase crop yields. Mg contents were also in high category (>351 mg kg-1). The K/Mg values ranged from 0.60 to 0.80 in Mollic Nitisols (aric, humic), 0.70 to 0.88 in Pellic Vertisols (aric, gilgaic, mazic) and 0.71 to 1.04 in Vertic Luvisols (aric, nitic), and the values increased with an increasing K rates. However, wheat, tef and maize grain yields showed an increasing trend up to K/Mg ratio of 0.71 in Mollic Nitisols (aric, humic), 0.78 in Pellic Vertisols (aric, gilgaic, mazic) and 0.88 in Vertic Luvisols (aric, nitic), respectively, and declined above these values.

A fine scale eco-epidemiological study on endemic visceral leishmaniasis in north ethiopian villages.

Visceral Leishmaniasis (VL) is a disseminated protozoan infection caused by Leishmania donovani that affects almost half a million people annually. In Northern Ethiopia, VL is common in migrant agricultural laborers returning from the lowland sesame fields of Metema and Humera. Recent VL foci have emerged in resident rural populations near the town. In the current study, we evaluate multilevel entomological, epidemiological and ecological factors associated with infection and disease through fine-scale eco-epidemiological analyses in three villages. Satellite images showed that villages constructed in or close to vertisols, were likely to become endemic for VL. Vertisols or black-cotton soil, are characterized by high contents of smectitic clay minerals, which swell when hydrated and shrink upon desiccation, causing extensive deep cracking during the dry season. The population densities of Phlebotomus orientalis, the vector, were negatively correlated with distance from vertisols and persons living close to vertisols were more likely to be bitten by sand flies, as evidenced by sero-positivity to Ph. orientalis saliva. Apparent (albeit non-significant) clustering of VL cases and abundant asymptomatic infections close to vertisols, suggest anthroponotic transmission around houses located close to vertisols. Comparable rates of male and female volunteers, mostly under 15 years of age, were infected with L. donovani but a significantly higher proportion of males succumbed to VL indicating a physiological gender-linked male susceptibility. Our data suggest that the abundant infected persons with high parasitemias who remain asymptomatic, may serve as reservoir hosts for anthroponotic transmission inside villages. Only limited insights on the transmission dynamics of L. donovani were gained by the study of environmental factors such as presence of animals, house structure and vegetation cover.