[Investigation of the chemical composition of fruit and berry raw materials of the Far Eastern Region as a perspective source of nutrients and bioactive compounds].

The Far Eastern region is rich in fruit and berry raw materials with a high content of bioactive substances. The aim of the work is to study the chemical composition of wild berries of the Amur region of the Far Eastern region. Material and methods. The objects of the research were the berries of Sargent viburnum (Viburnum sargentii Koechne), Chinese Schisandra [Schisandra chinensis (Turcz.) Baill], and Amursky grapes (Vitis amurensis Rupr) harvested from 2011 to 2016. The content of organic acids and tannins was determined by titration, anthocyanins - spectrophotometrically, polyphenolic substances and ß-carotene - photoelectrocolo rimetrically, vitamins B1, B2 - fluorometrically, vitamins B6 and E - by HPLC, hydroxycinnamine acids - by direct spectrophotometry. Results and discussion. The maximum amount of polyphenolic substances and vitamin C was found in V. sargentii berries, the average value of which was 1.01±0.04% and 85.2±2.4 mg/100 g, respectively. A high content of potassium was found in V. amurensis (an average of 188.5±2.2 mg/100 g) and V. sargentii (176.6±1.2 mg/100 g). The highest content of vitamins B1, B2, B6 was found in the berries of V. amurensis, the average content of which was, respectively, 0.071±0.002 mg/100 g, 0.065±0.002 mg/100 g and 0.081±0.004 mg/100 g. It was also found that in V. sargentii berries the level of all minerals and trace elements was the highest: calcium content in 100 g of berries was 41.4±0.8 mg, magnesium - 17.3±0.4 mg, phosphorus - 227.7±4.6 mg, manganese - 0.69±0.08 mg, iron - 0.60±0.08 mg, zinc - 0.90±0.08 mg. When consumed 100 g of V. sargentii berries, practically fully satisfies the need of an adult in vitamin C, in phosphorus and manganese - by about a quarter; in potassium, iron, and zinc, by 4-8%. 100 g of Sch. chinensis contains about 50% of the recommended daily intake of vitamin C, and 100 g of V. amurensis - about 10% of vitamin C and ß-carotene, 7% of potassium, about 4% of group B vitamins. The studied berries can serve as a source of hydroxycinnamic acids and pectins. Conclusion. Studies of the chemical composition of the berries V. sargentii, Sch. chinensis, V. amurensis harvested in 2011-2016 indicates a wide range of bioactive compounds, the proportion of each of which varies.

Integrated LC-MS/MS method and network pharmacology for exploring the characterization and mechanism of neuroprotective effect of Vitis amurensis Rupr. wine polyphenol.

Polyphenols are the main active components in Vitis amurensis Rupr. wine, which show good protective effects on the nervous system, but their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. The purpose of this study is to investigate the potential mechanism of the neuroprotective effect of Vitis amurensis Rupr. wine polyphenols on the basis of the specific composition of polyphenols in Vitis amurensis Rupr. wine. In this study, 40 phenolic compounds which include 15 anthocyanins, 10 flavonoids, 10 phenolic acids, 3 proanthocyanidins and 2 resveratrols were identified by UPLC Q-Exactive Orbitrap MS. Furthermore, An UPLC-QqQ/MS method was established to simultaneously determine the phenolic compounds in Vitis amurensis Rupr. Wine, and analyze the content differences of phenolic compounds between Vitis amurensis Rupr. and Vitis vinifera wine. Finally, network pharmacology was employed for the first time to predict the possible pharmacological mechanisms of Vitis amurensis wine polyphenols against nervous damage. Multivariate network analysis indicated that quercetin, (-)-epigallocatechin and various anthocyanins were found as prominent compounds for the treatment of nervous system diseases. Vitis amurensis Rupr. wine polyphenols mainly acted on these key targets, including AKT1, EGFR, ESR1, and SRC, and further regulate the PI3K-AKT and Rap1 signaling pathway for treating nervous system diseases. Our findings suggested that polyphenols in Vitis amurensis Rupr. wine possess neuroprotective effect through multicomponent, multitarget, and multichannel. PRACTICAL APPLICATIONS: Studies have revealed that Vitis amurensis Rupr. has higher levels of phenolic compounds than Vitis vinifera wine. However, due to the few and limited study of Vitis amurensis Rupr., their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. This research uses a holistic network pharmacology strategy to investigate the potential targets and pharmacological mechanisms of Vitis amurensis Rupr. wine polyphenols against nervous damage. To some things up, The finding elucidates the relationships between signaling pathways, targets, and compounds in Vitis amurensis Rupr. wine polyphenols, which may provide guidance and foundations for further application of medicinal food.

Leaf Removal at Véraison and Foliar K+ Application to Beibinghong Vines Improved Berry Quality under Cold-Climate Conditions.

(1) Background: Beibinghong is a grapevine variety that is well distributed in Northeastern China due to its adaptation to extreme cold conditions and vine diseases. Nonetheless, Beibinghong wines are extremely acidic and rich in phenolic compounds. The aim of this research was to study the effects of leaf removal at véraison and foliar K+ applications on Beibinghong vines to reduce the acidity and increase their polyphenol content. (2) Methods: Beibinghong berries were harvested when they reached close to 20 °Brix, and the physicochemical parameters were determined. (3) Results: Leaf removal at véraison plus K+ foliar applications to Beibinghong vines decreased the titratable acidity and increased the total phenolic and phenolic acid contents compared with the control. Moreover, the titratable acidity in the Beibinghong berries was negatively related to their total contents of phenols, proanthocyanidins, and anthocyanins. (4) Conclusions: Leaf removal at véraison performed with foliar K+ applications to vines could be an interesting alternative for Beibinghong production under cold-climate viticulture because it allows for a decrease in the acidity and an increase in the phenolic content of the berries, without incurring the risk of sunburn.

Effect of stabilization malvids anthocyanins on the gut microbiota in mice with oxidative stress.

Malvids anthocyanins have been proven to have a significant antioxidant activity. However, natural anthocyanins are unstable as they are easily affected by temperature, light, and pH. They can produce copigmentation with caffeic acids, leading to the improvement of color stability. The objective of this research was to survey the anti-oxidative stress functional role of stabilization malvids anthocyanins (SMA) in vivo. Changes on the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) in the serum and liver of oxidatively damaged mice of SMA were investigated. The effects of SMA on the diversity of gut microbiota in mice with oxidatively damage were also evaluated. Compared to oxidative damaged mice, SMA increased the activities of SOD, GSH-Px, CAT and T-AOC but decreased the levels of MDA in the serum and liver. SMA significantly changed the composition and diversity of the gut microbiota. Specifically, SMA increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes. At the genus level, SMA significantly increased the relative abundance of Lactobacillus, but decreased the relative abundance of Bacteroides. In addition, SMA also reversed carbohydrate metabolism and amino acid metabolism to normal levels. It indicates that SMA could protect the body from oxidative damage and be used as a potential functional food to prevent diseases related to oxidative stress. PRACTICAL APPLICATIONS: Anthocyanins provide protective effects against harmful effect of oxidative stress. Natural anthocyanins are safer and nutritious as compared to synthetic pigments. However, their stability is poor. The previous research done by this group showed that the anthocyanins content of variety of Vitis amurensis Rupr was as high as 180 mg/(100 g·FW), and the content of malvids anthocyanidin in its ingredients was the highest of all. Malvids anthocyanin and caffeic acid are bonded to produce stabilized malvids anthocyanins (SMA) high hydrostatic pressure technology, which has better stability. Our results indicate that SMA could increased the activities of antioxidant enzymes and altered the composition and diversity of the gut microbiota in mice with oxidative damage. The study will help to deepen the understanding of antioxidative stress mechanism of SMA and lay a foundation for the application of natural anthocyanidin in health aspect.

Vitis amurensis Rupr: A review of chemistry and pharmacology.

PURPOSE: Vitis amurensis Rupr. from the family Vitaceae, is a grape species native to the Asian continent. It is a highly attractive plant, used widely worldwide. It has been used for several hundred years as a traditional Chinese herb. The review focuses on the botanical description, traditional uses, phytochemistry, and the biological activities of Vitis amurensis Rupr. to evaluate its therapeutic potential uses. METHODS: This review summarizes the published data concerning the botanical aspects, traditional usage, phytochemistry, and pharmacology of Vitis amurensis Rupr., to evaluate its therapeutic potential as an important source of natural compounds with effect activities that benefit human health. RESULTS: Vitis amurensis Rupr. has been used for several hundred years as a traditional Chinese herb to treat stranguria, rheumatoid arthritis-associated edema, chronic hepatitis, nephritis, chronic arthritis and traumatic hemorrhage. It is a particularly rich source of the oligostilbenes, flavonoids, and anthocyanins, phytochemicals that are associated with antioxidant, anti-inflammatory, antibacterial and cardioprotective activities. Due to the presence of a multitude of bioactives, a wide array of pharmacological activities have been ascribed to different parts of this herb and individual compounds, which include antioxidant, antimicrobial, anti-inflammatory, anticancer, anti-aging, anti-melanogenic, anti-allergic and anti-viral. CONCLUSION: From a health perspective, Vitis amurensis Rupr. presents excellent options for treating various diseases due to its bioactive compounds (drug candidates) that exhibit important activities or for developing new products.

Isolation and structural identification of the main anthocyanin monomer in Vitis amurensis Rupr.

Many types of anthocyanins are present in Vitis amurensis Rupr of 'Beibinghong', which is grown in North-east China and has high antioxidant activity. However, the anthocyanin with the highest content in V. amurensis Rupr has not yet been identified. In this study, pulsed electric field extraction and semi-preparative liquid phase separation were used to isolate the anthocyanin monomer from 'Beibinghong'. UV-vis spectroscopy, FTIR spectroscopy, mass spectra and nuclear magnetic resonance were used to identify the anthocyanin monomer. The antioxidant activities of the anthocyanin monomer were also analysed. Malvidin-3,5-O-diglucoside was identified as the main anthocyanin in V. amurensis Rupr, which could be used as a raw material for its extraction. Furthermore, malvidin-3,5-O-diglucoside can be potentially used as a functional food, and a novel therapeutic and preventive agent for oxidative stress-related diseases. This study provides technical information for the future purification and structural identification of anthocyanins.