On periodic growth and shrinkage of glaciers in the Warwan sub-basin, western Himalaya, between 1990 and 2020.

Knowledge about glacier extent, dynamics, and characteristics are important for climate change attribution and prediction. Understanding on long-term dynamics and glacier inventory is crucial, particularly for the melt-dominated and latitudinally-diverse western Himalayan glacier basins. In this study, a temporal inventory is prepared for Warwan-sub basin (WSB), utilizing satellite imageries since the 1993 (Landsat TM: 1993; ETM+: 2001, 2008; OLI: 2020) and elevation model (SRTM DEM: 2000). The base inventory was generated for the year 2001 and systematically adjusted to the glacier situations in 1993, 2008, and 2020. Results indicate that in the year 2001, WSB in the western Himalaya included 84 glaciers (> 0.02 km2) covering an area of 187.9 ± 5.8 km2. The mapping (2001) further revealed a supraglacial debris cover of 15% of the glacierized area (28.2 ± 0.9 km2). Overall, the debris cover increased by 6% between 1993 and 2020. Temporal analyses clearly suggest a period of gain in the glacierized area (2001-2008) interspersed by the two phases of decline (1993-2001 and 2008-2020). Results specify a stronger decline in the glacierized area during 1993 to 2001 (197.03 ± 6.1 to 187.9 ± 5.8 km2) than between 2008 and 2020 (188.4 ± 5.9 to 182.8 ± 5.66 km2). Remarkably, the glacierized area increased from 187.9 ± 5.8 to 188.4 ± 5.8 km2 during 2001 to 2008. In view of widespread recession of regional glaciers, the gain in the area between 2001 and 2008 represents a peculiar characteristic of WSB that needs further detailed investigation. Further analyses suggest that low-altitude, east-facing, debris-free, steep-sloped, and small glaciers experienced greater loss in the area than large, debris-covered, north-facing, gently sloped, and high-altitude glaciers. Overall, the study at the sub-basin scale reveals inherent glacier dynamics with periodic increase and decrease in the glacierized area and a notable influence of non-climatic factors in regulating spatial heterogeneity and the rate of glacier changes.

Population genetic attributes of common leopard (Panthera pardus fusca) from Uttarkashi, Western Himalayas.

BACKGROUND: The common leopard (Panthera pardus fusca), which persists in most of its historic range, is experiencing steady population decline due to habitat loss, anthrophonic disturbances, illegal poaching for their body parts, and retaliatory killings in response to the leopard-human conflicts. METHODS AND RESULTS: We analysed 143 scats samples and identified 32 unique leopards following a selected panel of seven loci with cumulative PID sibs 5.30E-04. We observed moderate genetic diversity at nuclear (Ho = 0.600 ± 0.06) and mitochondrial markers (Hd = 0.569 ± 0.009; π = 0.001 ± 0.0002) and found sub-structuring in the leopard population at Uttarkashi, Western Himalayas. CONCLUSIONS: The present study exhibits the utility of non-invasive genetics in monitoring the leopard population and paves the path to investigate population genetic parameters in further studies.

Physiological and genomic evidence supports the role of Serratia quinivorans PKL:12 as a biopriming agent for the biohardening of micropropagated Picrorhiza kurroa plantlets in cold regions.

The medicinal herb, Picrorhiza kurroa Royle ex Benth has become endangered because of indiscriminate over-harvesting. Although micropropagation has been attempted for mass propagation of the plant, survival of in vitro plantlets under green house/open field poses a major challenge. Biopriming of micropropagated plantlets with plant growth-promoting rhizobacteria (PGPR) are among the successful methods to combat this problem. Serratia quinivorans PKL:12 was the best-characterized PGPR from rhizospheric soil of P. kurroa as it increased the vegetative growth and survival of the micropropagated plantlets most effectively. Complete genome (5.29 Mb) predicted genes encoding proteins for cold adaptation and plant growth-promoting traits in PKL:12. Antibiotic and biosynthetic gene cluster prediction supported PKL:12 as a potential biocontrol agent. Comparative genomics revealed 226 unique genes with few genes associated with plant growth-promoting potential. Physiological and genomic evidence supports S. quinivorans PKL:12 as a potential agent for bio-hardening of micropropagated P. kurroa plantlets in cold regions.

Assessment and current dynamics of nutrients with reference to nitrogen attributes in subalpine forests of Western Himalaya, India.

Nitrogen is an essential macronutrient necessary for a variety of physiological processes in plants, available in numerous fractions, each of which has direct relationship with the area's environmental circumstances since it causes the formation of these fractions. Seasonal and altitudinal variations in nitrogen concentration were found to have a significant impact. The soil of the western Himalaya is rich in a variety of nutrients, notably nitrogen, which was a current source of worry. The study was conducted in north western Himalaya, and different fractions of nitrogen, viz., available nitrogen, total nitrogen, ammonical nitrogen, and nitrate nitrogen, were estimated with reference to different parameters. Annual retention potential was evaluated among various fractions, which yielded positive results, with Site 1 demonstrating the highest retention potential across all seasons. At various depths and seasons of the year, ammonical and nitrate nitrogen levels fluctuated. The findings revealed that the soil is rich in nitrogen fractions with variation directly related to changes in carbon dioxide concentration. Site 1 had the highest values of all the fractions and Site 4 the lowest, whereas the C/N ratio varied between sites. The study determined that nitrogen fractions were present in sufficient quantities and play an important role in the maintenance and growth of natural forests as well as in the reduction of carbon dioxide levels in the atmosphere.

Assessment of runoff in Chandra river basin of Western Himalaya using Remote Sensing and GIS Techniques.

The runoff of Chandra river basin in the Himalayan India was assessed using a hydrological model combined with satellite remote sensing observations. During a test period between 2000 and 2015, in situ measurements of runoff and meteorological parameters were conducted in the glacial catchment areas of Sutridhaka and Chhotashigri. A good agreement was found between the observed and predicted runoff (correlation R2 > 0.8). The hydrological model was then used to simulate the runoff of Chandra River for a period of 2000 to 2015. Almost 68% of the predicted runoff occurred during the ablation period (May to September). A sensitivity study of the Chandra basin hydrology to a predicted warming climate of 1 to 4 K, toward the end of the century suggests that increased production of glacial melt water would have more impact on runoff than potential increase in precipitation. During the monsoon months (of June to August), increased runoff is predicted due to enhanced glacial melting but the runoff in other months to be lower than present mean runoff, except for the summer months (March to July).

Transcriptome repository of North-Western Himalayan endangered medicinal herbs: a paramount approach illuminating molecular perspective of phytoactive molecules and secondary metabolism.

Medicinal plants of the North-Western Himalayan region are known for their unprecedented biodiversity and valuable secondary metabolites that are unique to this dynamic geo-climatic region. From ancient times these medicinal herbs have been used traditionally for their therapeutic potentials. But from the last 2 decades increasing pharmaceutical demand, illegal and unorganized trade of these medicinal plants have accelerated the rate of over-exploitation in a non-scientific manner. In addition, climate change and anthropogenic activities also affected their natural habitat and driving most of these endemic plant species to critically endangered that foresee peril of mass extinction from this eco-region. Hence there is an urgent need for developing alternative sustainable approaches and policies to utilize this natural bioresource ensuring simultaneous conservation. Hither, arise the advent of sequencing-based transcriptomic studies significantly contributes to better understand the background of important metabolic pathways and related genes/enzymes of high-value medicinal herbs, in the absence of genomic information. The use of comparative transcriptomics in conjunction with biochemical techniques in North-Western Himalayan medicinal plants has resulted in significant advances in the identification of the molecular players involved in the production of secondary metabolic pathways over the last decade. This information could be used to further engineer metabolic pathways and breeding programs, ultimately leading to the development of in vitro systems dedicated to the production of pharmaceutically important secondary metabolites at the industrial level. Collectively, successful adoption of these approaches can certainly ensure the sustainable utilization of Himalayan bioresource by reducing the pressure on the wild population of these critically endangered medicinal herbs. This review provides novel insight as a transcriptome-based bioresource repository for the understanding of important secondary metabolic pathways genes/enzymes and metabolism of endangered high-value North-Western Himalayan medicinal herbs, so that researchers across the globe can effectively utilize this information for devising effective strategies for the production of pharmaceutically important compounds and their scale-up for sustainable usage and take a step forward in omics-based conservation genetics.

Genetic monitoring of Himalayan goral (Naemorhedus goral) from Western Himalayas, India.

BACKGROUND: Himalayan goral (Naemorhedus goral), solitary cliff-dwelling species and are distributed throughout the Indian Himalayan region. Its populations across the range are facing severe threats due to habitat loss, fragmentation and changes in the land-use patterns by various anthropogenic activities. METHODS AND RESULTS: We carried out genetic analyses of Himalayan goral using the mitochondrial control regions and microsatellite loci (n = 10) in the Uttarkashi district of Uttarakhand. We reported a moderate genetic diversity at nuclear (Ho 0.602 ± 0.057) and mitochondrial markers (Hd-0.6931 ± 0.053; π-0.0048 ± 001). Bayesian skyline plot indicates a sharp decline in the goral population in the last 100 years. CONCLUSIONS: Our results indicate the population of Himalayan goral in Uttarkashi is under panmictic condition, plausibly due to long-ranging behaviour. The present study laid the foundation for future non-invasive genetics monitoring and detailed population genetic assessment of goral from the entire range in the Western Himalayas.

Paludisphaera soli sp. nov., a new member of the family Isosphaeraceae isolated from high altitude soil in the Western Himalaya.

A novel strain of Planctomycetes, designated JC670T, was isolated from a high altitude (~ 2900 m above sea level) soil sample collected from Garhwal region in the Western Himalaya. Colonies of this strain were observed to be light pink coloured with spherical to oval shaped cells having crateriform structures distributed all over the cell surface. The cells divide by budding. Strain JC670T was found to grow well at pH 7.0 and pH 8.0 and to tolerate up to 2% NaCl (w/v). MK6 was the only respiratory quinone identified. The major fatty acids of strain JC670T were identified as C18:1ω9c, C18:0 and C16:0, and phosphatidylcholine, two unidentified phospholipids and six unidentified lipids are present as the polar lipids. The polyamines putrescine and sym-homospermidine were detected. Strain JC670T shows high 16S rRNA gene sequence identity (95.4%) with Paludisphaera borealis PX4T. The draft genome size of strain JC670T is 7.97 Mb, with G + C content of 70.4 mol%. Based on phylogenetic analyses with the sequences of ninety-two core genes, low dDDH value (20.6%), low gANI (76.8%) and low AAI (69.1%) results, differential chemotaxonomic and physiological properties, strain JC670T (= KCTC 72850T = NBRC 114339T) is recognised as the type strain of a new species of the genus Paludisphaera, for which we propose the name Paludisphaera soli sp. nov.

Satellite-observed glacier recession in the Kashmir Himalaya, India, from 1980 to 2018.

The study, first of its kind in the Kashmir Valley, uses a time series of satellite data (1980-2018) to determine the glacier health, which is critical for sustaining the perenniality of the rivers originating from the area. The role of topography, morphology and climate on the observed glacier recession was investigated. In total, 147 glaciers were mapped from 1980 image; ~ 72% of the glaciers have area ≤ 3 km2 and a majority of them (123) are having size < 1 km2. The glaciers have reduced from 101.73 ± 16.79 km2 in 1980 to 72.41 ± 4.7 km2 in 2018 showing a recession of 29.32 ± 12.09 km2 during the period (28.82%). The observed glacier loss is higher (0.77 ± 0.31 km2 a-1) compared with the other Himalayan regions. The results indicated that there is strong influence of altitude, aspect, slope and climate on glacier recession. The glaciers with area ≤ 1 km2 have receded significantly more (41.20 ± 6.20%) than the larger glaciers > 3 km2 in area (15.97 ± 5.13%). The glaciers situated between 4200 and 4400 m altitudes have receded more (~ 55 ± 5.01%) than those situated at altitudes > 4800 m (~ 19 ± 6.9%). Furthermore, the glaciers with steep slope (> 25) have witnessed lower recession (0.25 ± 0.15 km2 a-1) compared to the glaciers with gentle slope (0.51 ± 0.22 km2 a-1). The south-facing glaciers showed higher recession (~ 38%) compared with the north-facing glaciers (~ 27%). The findings suggest that the increase in temperature and decline in winter solid precipitation have resulted in the glacier recession with the consequent depletion of the streamflows, which, if continued in the future, would adversely affect the economy in the region.

Assessment of climate change pattern in the Pauri Garhwal of the Western Himalayan Region: based on climate parameters and perceptions of forest-dependent communities.

This study assessed the climate change in Pauri district, Uttarakhand, India, a region highly vulnerable to climate change with potentially high loss of livelihoods and lives. The scale of change in the district's climate was analyzed using meteorological station data (1901-2000) and grid data (1985-2015). Perceptions of climate change among forest-dependent communities in three altitude zones (< 1200 m asl (zone A); 1200-1800 m asl (zone B), and > 1800 m asl (zone C)) in the study region were surveyed with respect to 14 climate-specific indicators. Annual mean, maximum, and minimum temperature of seasonal data indicated increasing trends except monsoon. Percentage cloud cover showed an increase, of approximately 3%, while diurnal temperature displayed decreasing trends. Rainfall in the district showed a decreasing trend, with more than 50% of years 1985-2015 receiving less rainfall than the annual average. More than 90% of respondents in zones A and B, and around 65-70% respondents in zone C, reported changes in climate parameters. These findings confirm the long-term observable changes in climate in the region and demonstrate the utility of station data, grid data, and surveys of local communities' perceptions when analyzing climate change. The analysis provided important clues about the nature of climate changes in the district. The results can be used to reduce the gap between bottom-up understanding and top-down policies and to formulate precautionary and ongoing site-specific adaptation practices for communities in different altitude zones in the study region, leading to effective and efficient mitigation of climate change impacts.

Aerosol-associated n-alkanes over Dhauladhar region of North-Western Himalaya: seasonal variations in sources and processes.

Particulate n-alkanes are major constituents of organic aerosols (OA). Being primary in origin, chemically stable and thus long-lived, n-alkanes retains source signatures and along with diagnostic parameters have extensively been used to identify source(s) of OA. Systematic, yearlong study was carried out in the Dhauladhar region of North-Western Himalaya (NWH) to investigate dynamics in the composition and concentration of aerosol-associated n-alkanes. PM10 samples were collected for 24 h, once every week, at an urban mid-altitude location (Dharamshala) and a rural low-altitude site (Pohara). Particulate bound n-alkanes were identified and quantified using thermal desorption gas chromatography mass spectrometry (TD-GCMS). Annual mean concentrations of total n-alkanes (TNA) were 211 ± 99 ng m-3 and 223 ± 83 ng m-3, while mass fractions of TNA in PM10 were 4410 ± 1759 ppm and 3622 ± 1243 ppm at Dharamshala and Pohara, respectively. At both sites, a slight dominance of odd carbon-numbered n-alkanes was noticed. The TNA concentration and associated diagnostic parameters indicated unique source profiles at rural and urban locations. Significant seasonal variations were attributed to the contrasting land-use settings and meteorological variations. Influence of petrogenic contributions at urban location and predominance of biogenic contributions at rural location were observed in spring and autumn seasons. Preliminary insights on sources of organic aerosols are presented here. The diagnostic parameters allowed apportionment of biogenic and petrogenic sources. Biogenic emissions from agricultural practices viz. harvesting and threshing were predominant in the rural settings, while tourism-led anthropogenic contributions significantly add to petrogenic contributions in urban environment of the NWH region.

Diverse culturable bacterial communities with cellulolytic potential revealed from pristine habitat in Indian trans-Himalaya.

The Pangi-Chamba Himalaya (PCH) region is very pristine, unique, and virgin niche for bioresource exploration. In the current study, for the first time, the bacterial diversity of this region was investigated for potential cellulose degraders. A total of 454 pure bacterial isolates were obtained from diverse sites in the PCH region, and 111 isolates were further selected for 16S rDNA characterization based on ARDRA grouping. The identified bacteria belonged to 28 genera representing four phyla: Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. Pseudomonas was most abundant genus, followed by Bacillus, Geobacillus, Arthrobacter, Paenibacillus, and Flavobacterium. In addition, six putative novel bacteria (based on 16S rDNA sequence similarity) and thermophiles from non-thermogenic sites were also reported for the first time. Screening for cellulose degradation ability on carboxymethyl cellulose plates revealed that 70.92% of bacteria were cellulolytic. The current study reports diverse bacterial genera (Arthrobacter, Paenibacillus, Chryseobacterium, Pedobacter, Streptomyces, Agromyces, Flavobacterium, and Pseudomonas) with high capacity for cellulose hydrolysis and cellulolytic functionality at wide pH and temperature not previously reported in the literature. Diverse bacterial genera with high cellulolytic activity in broad pH and temperature range provide opportunity to develop a bioprocess for efficient pretreatment of lignocellulosic biomass, which is currently being investigated.

Do avian blood parasites influence hypoxia physiology in a high elevation environment?

BACKGROUND: Montane birds which engage in elevational movements have evolved to cope with fluctuations in environmental hypoxia, through changes in physiological parameters associated with blood oxygen-carrying capacity such as haemoglobin concentration (Hb) and haematocrit (Hct). In particular, elevational migrants which winter at low elevations, encounter varying intensities of avian haemosporidian parasites as they traverse heterogeneous environments. Whilst high intensity parasite infections lead to anaemia, one can expect that the ability to cope with haemosporidian infections should be a key trait for elevational migrants that must be balanced against reducing the oxygen-carrying capacity of blood in response to high elevation. In this study, we explored the links between environmental hypoxia, migration, and disease ecology by examining natural variation in infections status and intensity of avian haemoporidians across a suite of Himalayan birds with different migratory strategies while controlling for host phylogeny. RESULTS: We found predictably large variation in haemoglobin levels across the elevational gradient and this pattern was strongly influenced by season and whether birds are elevational migrants. The overall malaria infection intensity declined with elevation whereas Hb and Hct decreased with increase in parasite intensity, suggesting an important role of malaria parasites on hypoxia stressed birds in high elevation environments. CONCLUSIONS: Our results provide a key insight into how physiological measures and sub-clinical infections might affect dynamics of high-elevation bird populations. We suggest a potential impact of avian elevational migration on disease dynamics and exposure to high intensity infections with disease spread in the face of climate change, which will exacerbate hypoxic stress and negative effects of chronic avian malaria infection on survival and reproductive success in wild birds. Future work on chronic parasite infections must consider parasite intensity, rather than relying on infection status alone.

Assessment of snow-glacier melt and rainfall contribution to stream runoff in Baspa Basin, Indian Himalaya.

Hydrological regimes of most of the Himalayan river catchments are poorly studied due to sparse hydro-meteorological data. Hence, stream runoff assessment becomes difficult for various socio-industrial activities in the Himalaya. Therefore, an attempt is made in this study to assess the stream runoff of Baspa River in Himachal Pradesh, India, by evaluating the contribution from snow-ice melt and rainfall runoff. The total volume of flow was computed for a period of 15 years, from 2000 to 2014, and validated with the long-term field discharge measurements, obtained from Jaipee Hydropower station (31° 32' 35.53″ N, 78° 00' 54.80″ E), at Kuppa barrage in the basin. The observations suggest (1) a good correlation (r2 > 0.80) between the modeled runoff and field discharge measurements, and (2) out of the total runoff, 81.2% are produced by snowmelt, 11.4% by rainfall, and 7.4% from ice melt. The catchment receives ~75% of its total runoff in the ablation period (i.e., from May to September). In addition, an early snowmelt is observed in accumulation season during study period, indicating the significant influence of natural and anthropogenic factors on high-altitude areas.

Diversity of bioactive compounds and antioxidant activity in Delicious group of apple in Western Himalaya.

Apple contributes significantly to the livelihood and health of the people living in Himalayan regions. Among others, the Delicious group of apple is known for their health promoting and income generating attributes. However, the systematic investigation on morphological and phytochemical attributes of the apple growing in Indian Himalayan region is poorly known. An altitude-dependent variation in the fruit quality traits and phytochemical diversity was observed. The Royal Delicious was found rich in total tannin, flavonoids, flavonols, antioxidant activity [2,2-azinobis (3-ethylbenzothiazoline-6-sulphonic acid), 2,2-Diphenyl-1-picryhydrazyl, Ferric reducing antioxidant power], procyanidin B2, phloridzin and gallic acid; Red Delicious for total phenol and epicatechin and Golden Delicious for chlorogenic acid. The cultivar and altitude-dependent variation of phenolic profile in peel and pulp portion emphasizes genotype-specific biosynthesis of phenolic compounds in regulatory mechanisms. It is suggested that selection of suitable altitude and cultivars is required for harnessing the maximum phytochemical and antioxidant activity for benefits to human consumption.

Soil CO2 efflux among four coniferous forest types of Kashmir Himalaya, India.

Soil CO2 efflux was measured in four different coniferous forest types (Cedrus deodara (CD), Pinus wallichiana (PW), mixed coniferous (MC), and Abies pindrow (AP)) for a period of 2 years (April 2012 to December 2013). The monthly soil CO2 efflux ranged from 0.8 to 4.1 µmoles CO2 m(-2) s(-1) in 2012 and 1.01 to 5.48 µmoles CO2 m(-2) s(-1) in 2013. The soil CO2 efflux rate was highest in PW forest type in both the years, while it was lowest in MC and CD forest types during 2012 and 2013, respectively. Soil temperature (TS) at a depth of 10 cm ranged from 3.8 to 19.4 °C in 2012 and 3.5 to 19.1 °C in 2013 in all the four forest types. Soil moisture (MS) ranged from 19.8 to 58.6% in 2012 and 18.5 to 58.6% in 2013. Soil CO2 efflux rate was found to be significantly higher in summer than the other seasons and least during winter. Soil CO2 efflux showed a significant positive relationship with TS (R2=0.52 to 0.74), SOC% (R2=0.67), pH (R2=0.68), and shrub biomass (R2=0.51), whereas, only a weak positive relationship was found with soil moisture (R2=0.16 to 0.41), tree density (R2=0.25), tree basal area (R2=0.01), tree biomass (R2=0.07), herb biomass (R2=0.01), and forest floor litter (R2=0.02). Thus, the study indicates that soil CO2 efflux in high mountainous areas is greatly influenced by seasons, soil temperature, and other environmental factors.

Population genetic insights into the conservation of common walnut (Juglans regia) in Central Asia.

The common walnut (Juglans regia) is one of the most economically important nut trees cultivated worldwide. Despite its importance, no comprehensive evaluation of walnut tree population genetics has been undertaken across the range where it originated, Central Asia. In this study, we investigated the genetic diversity and population structure of 1082 individuals from 46 populations across Central Asia. We found moderate genetic diversity of J. regia across Central Asia, with 46 populations clustered into three groups with a weak relationship between genetic and geographic distance. Our findings reveal that the western Himalaya might be the core region of common walnut genetic diversity in Central Asia and that, except for two populations in Gongliu Wild Walnut Valley, humans might have introduced walnut populations to Xinjiang, China. The observed distribution of the genetic landscape has probably been affected by historical climate fluctuation, breeding system, and prolonged anthropogenic activity. We propose the conservation of the core genetic diversity resources in the western Himalaya and pay special attention to populations from Gongliu in Xinjiang. These findings enhance our understanding of the genetic variation throughout the distribution range of J. regia in Central Asia, which will provide a key prerequisite for evidence-based conservation and management.

Glacial lake outburst flood risk assessment using remote sensing and hydrodynamic modeling: a case study of Satluj basin, Western Himalayas, India.

Glacier-associated hazards are becoming a common and serious challenge to the high mountainous regions of the world. Glacial lake outburst floods (GLOFs) are one of the most serious unanticipated glacier hazards, with the potential to release a huge amount of water and debris in a short span of time, resulting in the loss of lives, property, and severe damage to downstream valleys. The present study used multi-temporal Landsat and Google earth imageries to analyze the spatio-temporal dynamism of the selected glacial lake (moraine-dammed) in the Satluj basin of Western Himalayas. Furthermore, GLOF susceptibility of the lake was assessed using a multi-criteria decision-based method. The results show that the lake area has increased from 0.11 to 0.26 km2 over the past 28 years from 1990 to 2018. The susceptibility index value for the lake was calculated as 0.75, which indicates that the lake is highly susceptible to the GLOF. The depth and volume of the lake were estimated to be 16 m and 57 × 105 m3, respectively, using an empirical formula. HEC-RAS, HECGeo-RAS, and Arc-GIS software were utilized in this study to perform unsteady flow analysis and to determine the GLOF impact on the downstream area. The worst-case GLOF scenario (breach width of 75 m) was revealed during an overtopping failure of the moraine dam, resulted in a peak discharge of 4060 m3/s and releasing a total water volume of 57 × 105 m3. The breach hydrograph has been routed to calculate the spatial and temporal distribution of peak flood, inundation depth, velocity, water surface elevation, and flood peak arrival time along the river channel. The analysis further reveals that the routed flood waves reach the nearest settlement, i.e., Rajpur town, situated at a distance of 102 km in the downstream valley of the lake at 6 h after the beginning of the lake breach event with a peak discharge/flood of 1757 m3/s and maximum flow velocity of 1.5 m/s. With the ongoing climate warming and glacier retreat, moraine-dammed lakes are becoming more hazardous and thus increasing the total threat. Therefore, it is mandatory to monitor and assess such lakes at regular intervals of time to lessen the disastrous impacts of GLOFs on the livelihood and infrastructure in the downstream valleys. The findings of this study will aid in the creation of risk management plans, preparatory tactics, and risk reduction techniques for GLOF hazards in the region.

Challenges and opportunities under COVID-19 on rural populace in Kailash Sacred Landscape (KSL)-India.

More than just a health crisis, COVID-19 pandemic has stressed across social, economical and emotional dimensions of human well being and health. The sudden enforcement although willful and honest, perpetuated a sense of insecurity and uncertainty as a result of livelihood loss, especially for the people employed in unorganized and private sectors across different urban centers of the country. The unexpected scenario not only caused widespread joblessness but also created varied conditions of psychological stresses foreseeing the less likelihood of returning of pre-COVID conditions. As an outcome of the pandemic, the involuntarily return of youth was seen as a boon for reversing the undesirable and unprecedented trends impacting the traditional rural dynamics. Present study is an attempt to highlights impacts, challenges and opportunities under and after COVID-19 on rural populace of Kailash Sacred Landscape (KSL) amid the sudden halt of remittances and jobless aspiring youth. A systematic approach was followed, where 16 villages in eight Blocks of KSL were assessed and migrants (n=815) were interviewed for compiling the information. Results revealed that COVID-19 has impacted (both positively and negatively) various sectors such as agriculture, tourism, overall family income, etc., as perceived by the respondents. Sectors like restaurants/shops, tourism, and agriculture emerged as potential cash generating avenues, with 30 %, 21 %, and 20 % respondent's perception, respectively. It is also revealed that in the absence of immediate alternatives, and policy intervention, villagers engaged themselves in the time tested traditional sectors of livelihoods or created their own niche by integrating traditional wisdom and acquired skills.

Exploring the thermal limits of malaria transmission in the western Himalaya.

Environmental temperature is a key driver of malaria transmission dynamics. Using detailed temperature records from four sites: low elevation (1800), mid elevation (2200 m), and high elevation (2600-3200 m) in the western Himalaya, we model how temperature regulates parasite development rate (the inverse of the extrinsic incubation period, EIP) in the wild. Using a Briére parametrization of the EIP, combined with Bayesian parameter inference, we study the thermal limits of transmission for avian (Plasmodium relictum) and human Plasmodium parasites (P. vivax and P. falciparum) as well as for two malaria-like avian parasites, Haemoproteus and Leucocytozoon. We demonstrate that temperature conditions can substantially alter the incubation period of parasites at high elevation sites (2600-3200 m) leading to restricted parasite development or long transmission windows. The thermal limits (optimal temperature) for Plasmodium parasites were 15.62-34.92°C (30.04°C) for P. falciparum, 13.51-34.08°C (29.02°C) for P. vivax, 12.56-34.46°C (29.16°C) for P. relictum and for two malaria-like parasites, 12.01-29.48°C (25.16°C) for Haemoproteus spp. and 11.92-29.95°C (25.51°C) for Leucocytozoon spp. We then compare estimates of EIP based on measures of mean temperature versus hourly temperatures to show that EIP days vary in cold versus warm environments. We found that human Plasmodium parasites experience a limited transmission window at 2600 m. In contrast, for avian Plasmodium transmission was not possible between September and March at 2600 m. In addition, temperature conditions suitable for both Haemoproteus and Leucocytozoon transmission were obtained from June to August and in April, at 2600 m. Finally, we use temperature projections from a suite of climate models to predict that by 2040, high elevation sites (~2600 m) will have a temperature range conducive for malaria transmission, albeit with a limited transmission window. Our study highlights the importance of accounting for fine-scale thermal effects in the expansion of the range of the malaria parasite with global climate change.