Biochar and saline soil: mitigation strategy by incapacitating the ecological threats to agricultural land.

Soil salinity caused a widespread detrimental issue that hinders productivity in agriculture and ecological sustainability, while waste-derived soil amendments like biochar have drawn attention for their capacity to act as a mitigating agent, by enhancing the physical and chemical features of soil, and contributing to the recovery of agricultural waste resources. However, the information concerning biochar and salinity which affect the physicochemical characteristics of soils, crop physiology, and growth is limited. To investigate whether biochar mitigates the salinity stress on wheat crop seedlings, we grow them with salinity stress (120 mM), and biochar (20 tons ha-1), and its interactive effects. The soil properties of soil organic carbon (SOC), soil organic matter (SOM), dissolved organic carbon (DOC), and soil available phosphorus (SAP) decreased in the saline soil by 36.71%, 46.97%, 26.31%, and 15.00%, while biochar treatment increased SOC, DOC, and SAP contents by 7.42%, 31.57%, and 15.00%, respectively. On the other hand, dissolved organic nitrogen (DON) contents decreased in all the treatments compared to the control. The root growth traits, SPAD values, leaf nitrogen, photosynthetic parameters, antioxidant enzymes, and reactive oxygen species decreased in the saline treatment while increasing in the biochar and interactive treatment. Thus, these activities resulted in higher leaves and root biomass in the biochar treatment alone and interactive treatment of salinity and biochar. According to principal component analysis, redundancy analysis, and the mantel test, using biochar in conjunction with salinity treatment was found to be more effective than salinity treatment alone. The results of this study suggest that biochar can be used as a sustainable agricultural technique and a means of mitigation agent by lowering soil salinity while increasing the biomass of crops.

Biochar improves the physical and nutritional quality of soil and plant function.Salinity stress declined the physiological activities and biomass of the crop.Biochar mitigates the salinity stress in soil and enhances the plant functioning.Exposure to both treatments enhances the antioxidant enzyme activity and biomass.

Synthesis, characterization and antifungal potential of titanium dioxide nanoparticles against fungal disease (Ustilago tritici) of wheat (Triticum aestivum L.).

Nanoparticles (NPs) preparation using a green as well as environmentally acceptable processes has achieved a lot of attention in recent decade. The current study compared the synthesis of titania (TiO2) nanoparticles synthesized from leaf extracts of two plant species (Trianthema portulacastrum, Chenopodium quinoa) and traditional approach by chemical preparation. The effects of no calcination on the physical characteristics of TiO2 NPs as well as their antifungal effects were examined and compared with the already reported calcinated TiO2 NPs. The produced TiO2 NPs were evaluated using high-tech techniques such as X-ray diffraction (XRD), scanning electron microscope, energy dispersive spectroscopy (EDX), and elemental mapping. TiO2 NPs prepared by sol-gel technique (T1) and prepared from extractions from leaves of T. portulacastrum (T2), and C. quinoa (T3) were either calcinated or non calcinated and tested against fungal disease (Ustilago tritici) of wheat for antifungal efficacy. The -peak (2θ) at 25.3 was confirmed by XRD to be connected with the anatase (101) form in both cases but before calcination, NPs were lacking the rutile and brookite peaks. The results showed that all types of TiO2 NPs examined had good antifungal activity against U. tritici, but those made from C. quinoa plant extract have good antifungal activity against disease. TiO2 NPs which are produced by the green methods (T2, T3) have the highest antifungal activity (58%, 57% respectively), while minimal activity (19%) was recorded when NPs were synthesized using the sol-gel method (T1) with 25 µl/mL. Non calcinated TiO2 NPs have less antifungal potential than calcined TiO2 NPs. It can be concluded that calcination may be preferred for efficient antifungal activity when using titania nanoparticles. The green technology may be used on a larger scale with less damaging TiO2 NP production and can be utilized against fungal disease on wheat crop to reduce crop losses worldwide.

Immobilization of cadmium in soil-plant system through soil and foliar applied silicon.

We conducted a pot experiment to evaluate the potential for soil- and foliar-applied silicon (Si), alone and in combination, to a Cd-contaminated soil in order to evaluate the effects on such amendments on the Cd translocation from soil to wheat root, shoot and grains. Five treatments were used, T1) control with no external factor added, T2 received only Cd, while T3-T5 treatments received Cd in combination with soil, foliar and soil plus foliar applied Si. Except control (T1), soil was contaminated with Cd at 10 mg kg-1 in all the treatments and 1% solution of Si as an amendment was used for soil and/or foliar application or their combination. Overall, while Si application improved both plant growth and yield in Cd-contaminated soil. Control and combined soil- and foliar-applied Si in Cd contaminated treatments showed equally positive (2.5%) increase in plant height over Cd contaminated treatment. Grain yield was also highest in the treatment receiving Cd plus soil-applied Si (29%) followed by control (26%). It was concluded that Si can alleviate Cd toxicity in wheat irrespective of whether the Si was soil-applied or applied via a foliar method, but soil applied Si proved the best in this regard.Novelty statement Immobilization of metals i.e., cadmium (Cd) with soil-applied amendments like biomaterials and organic manure to decrease Cd concentration in plants have already been widely investigated. Silicon (Si) is a cheap in-organic and readily available element in the nature and also used for the same purpose. It can be applied both in soil as well as by foliar and soil + foliar application to decrease the metals concentration in soil and plants. However, comparative effectiveness of these three methods have not been checked simultaneously. In this study, we have studied the comparative effectiveness of Si application to soil, foliar and their combination (soil + foliar) to decrease Cd concentration during wheat crop.

Shifts in biochemical and physiological responses by the inoculation of arbuscular mycorrhizal fungi in Triticum aestivum growing under drought conditions.

BACKGROUND: A decrease in rainfall is one of the main constraints on wheat production, although the association of wheat with arbuscular mycorrhizal fungi (AMF) may be an alternative for crop production under drought conditions. In the present study, we used three wheat cultivars (Purple, Ilustre and Maxi Baer) inoculated with two AMF strains, one obtained from the hyperarid Atacama Desert (northern Chile; Fm) and the other obtained from southern Chile (Cc). Plants were maintained under two irrigation conditions (normal irrigation and drought) and the physiological behaviour and enzymatic and nonenzymatic antioxidant activities in the shoots were determined. In addition, the phenolic compounds were identified by high-performance liquid chromatography-dioide array detection-electrospray ionization-tandem mass spectrometry and quantified. RESULTS: AMF colonization produced higher levels of efficiency of photosystem II and photosynthetic pigments. High values of catalase in Purple-Cc, ascorbate peroxidase in Purple-Cc, glutathione reductase in Maxi-Cc and superoxide dismutase in Purple-Cc, all under stress, were registered. Of the inoculated cultivars, Purple-Cc showed the highest flavonoid levels, whereas hydroxycinnamic acids were higher in Maxi-Fm without drought, with apigenin and luteolin being the most abundant. High levels of phenols were present in the Ilustre-Fm plants without drought. Under normal irrigation, high levels of antioxidant activity were registered in the AMF treatments, whereas, under stress conditions, in general, high values were observed under the Fm inoculation. CONCLUSION: Our results showed that the greatest antioxidant activity and phenolic content occurred in wheat plants inoculated with AMF, indicating their influence on coping with water stress, which is of importance in vast areas where global climate change is resulting in diminished rainfall. © 2021 Society of Chemical Industry.

Fusarium Crown Rot Whitehead Symptom as Influenced by Wheat Crop Management and Sampling Date.

Symptoms of Fusarium crown rot of wheat include premature death of inflorescens (whiteheads), lesions on subcrown internodes, and rotting of crown tissue and lower stem internodes. Each symptom type is influenced by a different set of environmental conditions. Whiteheads are the easiest symptom to quantify and are frequently reported in the Pacific Northwest U.S.A. The objective of this research was to examine factors associated with whitehead expression and relationships with wheat yield and test weight. Incidence of whiteheads differed for inoculations with different isolates of F. pseudograminearum and F. culmorum, and over years due to weather factors. Whiteheads became less as planting dates for winter wheat were delayed until after September, and incidence was increased with increasing nitrogen application rate. Dates of initial and greatest expression of whiteheads differed among cultivars, which was associated in part with the cultivar heading date. Whiteheads were not correlated with subcrown internode lesions or browning of crown tissue. Whiteheads were also not correlated with grain test weight. Whiteheads were sometimes negatively associated with grain yield, but that relationship was variable and could not be considered a reliable, recurrent, or accurate measure of crown rot severity. These results indicate the need for caution in reporting whiteheads as a sole indicator of cultivar susceptibility to Fusarium crown rot.

Socio-demographic characters, distribution and transformation of iodine in soil, plant and wheat grains at District Diamer, Gilgit-Baltistan, Pakistan.

Iodine deficiency in soils and plants is a common problem in the hilly areas and has still been ignored. The aim of the current study is to investigate associated health issues, socio-economic characteristics and people's perception regarding iodine deficiency. Furthermore, a follow-up study was conducted to determine iodine and nutrients concentration in soil and crops at District Diamer, Gilgit-Baltistan, Pakistan. A descriptive and empirical analysis was examined. Most importantly, male was significantly observed as more affected for iodine deficiency than female, as contrary to the literature, socio-economic factors such as households' income and awareness (education) are reported as negative and significant contributor to reduce the iodine deficiency among the people irrespective of the gender. Majority of people (84%) heard about the iodine, but about 51.6% people argue that iodine has no effect on the human body and 56% of the respondents do not know why iodine is necessary for human. It was found that 11.5% of the community was affected from the IDD and majority of them were females. Wheat crops were the principal crop since it supplies 75% of calorific energy in an average Pakistani diet. The concentrations of iodine in soil samples range from 4.21 to 5.45 mg kg-1. The concentration of iodine in wheat crop plant sampled were considerably varied as Boner > Gais > Goher Abad with 1.12, 0.91 and 0.81 mg kg-1. respectively. Likewise, grain iodine concentration was varied as Gais > Boner > Goher Abad with 0.126, 0.102 and 0.078 mg kg-1, respectively. This study exposed that community using the cereal crops could face iodine deficiency in their diet which can cause endemic goiter among the population and control the iodine deficiency by empowering the rural community to raise the income level and providing the awareness to the people.

An Environmentally Friendly Engineered Azotobacter Strain That Replaces a Substantial Amount of Urea Fertilizer while Sustaining the Same Wheat Yield.

In our endeavor to improve the nitrogen fixation efficiency of a soil diazotroph that would be unaffected by synthetic nitrogenous fertilizers, we have deleted a part of the negative regulatory gene nifL and constitutively expressed the positive regulatory gene nifA in the chromosome of Azotobacter chroococcum CBD15, a strain isolated from the local field soil. No antibiotic resistance gene or other foreign gene was present in the chromosome of the engineered strain. Wheat seeds inoculated with this engineered strain, which we have named Azotobacter chroococcum HKD15, were tested for 3 years in pots and 1 year in the field. The yield of wheat was enhanced by ∼60% due to inoculation of seeds by A. chroococcum HKD15 in the absence of any urea application. Ammonium only marginally affected acetylene reduction by the engineered Azotobacter strain. When urea was also applied, the same wheat yield could be sustained by using seeds inoculated with A. chroococcum HKD15 and using ∼85 kg less urea (∼40 kg less nitrogen) than the usual ∼257 kg urea (∼120 kg nitrogen) per hectare. Wheat plants arising from the seeds inoculated with the engineered Azotobacter strain exhibited far superior overall performance, had much higher dry weight and nitrogen content, and assimilated molecular 15N much better. A nitrogen balance experiment also revealed much higher total nitrogen content. Indole-3-acetic acid (IAA) production by the wild type and that by the engineered strain were about the same. Inoculation of the wheat seeds with A. chroococcum HKD15 did not adversely affect the microbial population in the field rhizosphere soil.IMPORTANCE Application of synthetic nitrogenous fertilizers is a standard agricultural practice to augment crop yield. Plants, however, utilize only a fraction of the applied fertilizers, while the unutilized fertilizers cause grave environmental problems. Wild-type soil diazotrophic microorganisms cannot replace synthetic nitrogenous fertilizers, as these reduce atmospheric nitrogen very inefficiently and almost none at all in the presence of added nitrogenous fertilizers. If the nitrogen-fixing ability of soil diazotrophs could be improved and sustained even in the presence of synthetic nitrogenous fertilizers, then a mixture of the bacteria and a reduced quantity of chemical nitrogenous fertilizers could be employed to obtain the same grain yield but at a much-reduced environmental cost. The engineered Azotobacter strain that we have reported here has considerably enhanced nitrogen fixation and excretion abilities and can replace ∼85 kg of urea per hectare but sustain the same wheat yield, if the seeds are inoculated with it before sowing.

Genomic insights of a native bacterial consortium for wheat production sustainability.

The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium (Bacillus cabrialesii subsp. cabrialesii TE3T, Priestia megaterium TRQ8, and Bacillus paralicheniformis TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through 15N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha-1) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123-225 kg N ha-1 increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha-1 (6.0 vs. 7.1 ton ha-1, 0 kg N ha-1 added, 123 kg N ha-1 in the soil) and of 2.0 ton ha-1 (5.9 vs. 7.9 ton ha-1, 120 kg N ha-1 added, 104 kg N ha-1 in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO2 fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha-1) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha-1 was applied compared to when wheat was fully fertilized (240 kg N ha-1). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat.

Effect of green synthesized cerium oxide nanoparticles on fungal disease of wheat plants: A field study.

Recently, there has been considerable attention towards the production of environmentally friendly nanoparticles (NPs). In this investigation, the successful synthesis of cerium oxide nanoparticles (CeO2 NPs) was achieved by employing an eco-friendly technique that utilized an extract from the leaves of local plant quinoa (Chenopodium quinoa L.). The synthesized CeO2 NPs were subjected to characterization using state-of-the-art methods. The prepared CeO2 NPs contained a round shape with clusters and have a size of 7-10 nm. To assess how effective CeO2 NPs derived from C. quinoa were against Ustilago tritici, a fungal disease that negatively affects wheat crop globally, a study was performed on two varieties of wheat crop comprised of Arooj (V1) and Akber (V2), cultivated under field conditions. CeO2 NPs were applied foliarly twice to the wheat crop at four different concentrations: T0 (0 mg/L), T1 (50 mg/L), T2 (75 mg/L), and T3 (100 mg/L). The results revealed that the control group (T0) exhibited the highest disease severity index (DSI) with a value of 75% compared to the other concentrations of CeO2 NPs on both varieties. At a concentration of 100 mg/L of CeO2 NPs, the DSI dropped to a minimum of 35% and 37% on both V1 and V2 respectively. These findings indicated that an increase in the concentration of CeO2 NPs has a beneficial impact on disease severity. Similar patterns have also been observed with disease incidence (DI), with the greatest efficacy observed at a concentration of 100 mg/L of CeO2 NPs. Our investigation has shown that CeO2 NPs exhibitd significant antifungal potential against U. tritici which may be a promising strategy to mitigate fungal disease and crop losses globally.

Potentials of legumes rotation on yield and nitrogen uptake of subsequent wheat crop in northern Ethiopia.

Nitrogen has becoming the most limiting nutrient in the northern highlands of Ethiopia due to continuous cropping with application of limited external inputs. To improve soil nutrient availability, farmers have been using legumes in crop rotation. However, the roles of various legumes on subsequent wheat (Triticum aestivum) crop are unknown in northern Ethiopia. The objective of this study was to investigate impacts of legumes on yield and N uptake of subsequent wheat crop. Experiment was conducted at farmer's field with faba bean (Vicia faba L.), 'dekeko' field pea (Pisum sativum var. abyssinicum), field pea (Pisum sativum), lentil (Lens culinaris) and wheat (Triticum spp.) in the first season and all plots were rotated by wheat in the second season. Yield of subsequent wheat crop was recorded and N uptake was analyzed. The result revealed that grain yield and dry biomass yields of subsequent wheat crop were significantly (p < 0.05) higher in the legume-wheat rotations than in the wheat-wheat rotation. The wheat yield is increased by 2196, 1616, 1254 and 1065 kg ha-1 and the N uptake is increased by 71.4%, 51.0%, 49.2% and 29.8% in the faba bean-wheat, 'dekeko'-wheat, field pea-wheat and lentil-wheat rotation plots compared to the wheat continuous cropping, respectively. The findings indicated that legumes improved yield and N uptake of the subsequent wheat crop. Thus, soil fertility management policy need to consider legume crop rotations as nutrient management option to improve sustainable soil fertility and yield.

Evaluation of nanoceria on cadmium uptake in Triticum aestivum (L.) and its implications for dietary health risk.

In recent times, significant attention has been directed toward the synthesis and application of nanoparticles (NPs) in agriculture sector. In current study, nanoceria (CeO2 NPs) synthesized by green method were employed to address cadmium (Cd) accumulation in wheat (Triticum aestivum L.) cultivated in field with excess Cd. The application of CeO2 NPs was carried out through foliar spraying, performed twice during the growth of T. aestivum. Four levels of CeO2 NPs were used: T0, T1, T2, and T3 as 0, 50, 75, and 100 mgL-1, respectively. Results highlighted the positive effects of CeO2 NPs on various growth parameters, including plant height, spike length, photosynthetic related attributes, as well as straw and grain of grains in comparison to T1 (control group). Furthermore, CeO2 NPs led to a reduction in oxidative stress in the leaves and enhanced in enzyme activities in comparison to T1. Notably, Cd concentrations in straw, roots, and grains exhibited a decline following the treatment with CeO2 NPs, in contrast to the control group. In terms of health implications, the calculated health risk index associated with dietary consumption of grains by adults remained below the defined threshold with supply of nanoparticles. Foliar application of CeO2 NPs proved to be an effective approach in reducing cadmium content in wheat grains. This reduction holds significant potential for minimizing the risk of cadmium exposure to human health through the food chain. Employing the green synthesis method amplifies the potential for extensive production and a wide array of environmental applications for CeO2 NPs. This dual capacity makes them proficient in tackling environmental stresses while concurrently mitigating adverse ecological effects.

The effect of gibberellic acid on wheat growth and nutrient uptake under combined stress of cerium, zinc and titanium dioxide nanoparticles.

Nanoparticles (NPs) are released and dispersed in the environment because of increased manufacturing and use of nano products. NPs disturb the growth of plants depending upon types, exposure duration and plant species. The purpose of this research was to explore the role of gibberellic acid (GA) exposure through foliar route on wheat growth under alone or combined soil application of cerium oxide (CeO2), zinc oxide (ZnO), and titanium dioxide (TiO2) NPs. GA was foliar-applied (200 mg/L) on the wheat plants treated with individual and in all possible combination of the selected NPs. Explorations have revealed that the combination of NPs and GA worked well to enhance the plant growth and selected nutrient status than NPs alone. Furthermore, GA decreased the boosted antioxidant enzyme activities under the combination and individual NPs compared to the alone NPs treated plants, lowered the oxidative stress in wheat plants which provided the additional proof that GA decreased oxidative damage in plants. Combined NPs showed differential effects than individual NPs application irrespective of GA exposure which varied with NPs combination and studied parameters of plants. GA + NPs differentially affected the potassium, phosphorus, iron and manganese concentrations in wheat tissues than NPs alone treatments. Overall, GA can be applied when there is excess of NPs (either alone or in combination) in the growth medium to ensure the growth of crops. However, further studied are needed with other plant species and alone or combined use of different NPs under GA treatment before any final recommendation.

Zinc oxide nanoparticles coated urea enhances nitrogen efficiency and zinc bioavailability in wheat in alkaline calcareous soils.

Nitrogenous fertilizers have low efficiency in alkaline calcareous soils due to volatilization and denitrification. These losses cause economic environmental constraints. Coating of urea with nanoparticles (NPs) is an innovative strategy to improve crop yields by sustaining N availability. In the current study, zinc oxide nanoparticles (ZnO NPs) were synthesized by precipitation method and characterized for morphology and configuration, bond formation, and crystal assemblage using the X-ray diffraction and scanning electron microscope (SEM). The SEM results confirmed the size of ZnO NPs in the size range of 25 nm with cuboid shape. Urea fertilizer, coated with ZnO NPs, was applied to wheat crop in a pot trial. Two rates of ZnO NPs at 2.8 and 5.7 mg kg-1 were selected to coat the commercial urea. A batch experiment was conducted to ensure the ammonium (NH4+) and nitrate (NO3-) ions release by amending the soil with ZnO NPs coated urea and comparing with non-amended soil. The gradual release of NH4+ was observed for 21 days from the ZnO NP-coated urea. In the second part of trial, seven different treatments of coated and uncoated urea were tested on wheat crop. Urea coated with ZnO nanoparticles at 5.7 mg kg-1 improved all growth attributes and yields. The ZnO NP coated urea increased the N content shoot (1.90 g 100g-1 DW) and potentially biofortified Zn content (47.86 mg kg-1) in wheat grain. The results are indicative of viability of a novel coating for commercial urea that will not only reduce N losses but also supplement Zn without additional cost of labor.

Methylcellulose/lignin biocomposite as an eco-friendly and multifunctional coating material for slow-release fertilizers: Effect on nutrients management and wheat growth.

To obviate adverse effects from the non-biodegradability of certain polymer-based slow-release fertilizers (SRFs) and to offset higher operational costs, the use of biopolymers as coating material has recently caught interest in the research circles. The present work aims to design a sustainable coating material based on biodegradable polymers. To this end, Alfa plant was initially exploited as a viable sustainable source for the extraction of lignin (LGe), which was in turn integrated into the development of a three-dimensional cross-linked network, including methylcellulose (MC) as a matrix and citric acid (CA) as a cross-linking agent. Then, the designed coating material was applied onto Di-ammonium Phosphate (DAP) and Triple Superphosphate (TSP) water-soluble fertilizers in a rotating pan machine. Chemical, physical, and biodegradation studies have confirmed that the coating material is environmentally-friendly. Nutrients release experiments in water as well as in soil environments have proved the effectiveness of the MC and MC/LGe coating layers in delaying the nutrients discharge. Besides, the nutrients release from coated DAP and TSP lasted longer than 30 days. Furthermore, the coating film enhanced the fertilizers mechanical resistance and boosted the soil water retention capacity. The agronomic evaluation has also confirmed their remarkable potential in enhancing wheat leaf area, chlorophyll content and biomass, in addition to the roots architecture and the final fruiting efficiency. These results showed that this hybrid composite could be used as an efficient coating material to produce slow-release fertilizers with multifunctional performances.

Effects of the nitrification inhibitor nitrapyrin and mulch on N2O emission and fertilizer use efficiency using 15N tracing techniques.

Nitrous oxide (N2O), is a potent greenhouse gas (GHG) that shares 7% of global warming around the world. Among different sources, agricultural systems account for approx. 60% of global anthropogenic N2O emissions. These N2O emissions are associated with the activity of nitrifiers and denitrifiers that contribute to >4 Tg (teragrams) N2O-N emission per year. Application of nitrogen (N) fertilizers and manures in agricultural fields plays an imperative role in this regard. On the other hand nitrification inhibitors are an effective approach to minimize N2O-N emissions from agricultural fields. Here we examined the effects of applying urea with a nitrification inhibitor (Ni) nitrapyrin and mulch (Mu) on urea transformation, nitrous oxide (N2O) emissions, grain yield and nitrogen (N) uptake efficiency. The treatments include a control (zero N), urea (U) applied at 200 kg N ha-1, U + Ni (Ni applied at 700 g ha-1), U+ Mu (Mu applied at 4 t ha-1) and U + Ni + Mu. The N2O emission factor (EF) was 66% and 75% when U and Mu were applied, respectively. Yield-scaled N2O emissions were lower in U and Mu by 45% and 55%, respectively. The Ni coupled with Mu enhanced urea-15N recovery by 58% and wheat grain yield by 23% and total N uptake by 30% compared with U alone. In conclusion, Ni usage is an effective strategy to mitigate N2O emissions under field conditions.

Effect of biochars on bioaccumulation and human health risks of potentially toxic elements in wheat (Triticum aestivum L.) cultivated on industrially contaminated soil.

In the present study, biochars (BCs) derived from naturally grown green waste (Cynodon dactylon L.) were investigated regarding their impacts on bioaccumulation of potentially toxic elements (PTEs), agronomic properties and human health risks of wheat crop cultivated on long-term industrially contaminated soil. Typically, three types of BCs were pyrolyzed at different highest temperature of treatment (HTT), i.e. 400 °C, 600 °C and 800 °C, in a horizontal reactor and applied to the contaminated soil with 2% and 5% (w/w) ratio. The characterization results of the BCs showed that significant positive changes in fundamental characteristics such as porosity, surface area, cation exchange capacity, dissolved organic carbon, phosphorus and potassium have occurred with increased HTT. The analytical results of wheat crop indicated that the BCs applications significantly (p ≤ 0.05) reduced concentration of PTEs in roots (48-95%), shoots (38-91%), leaves (30-91%) and grains (38-93%) of wheat plants. After the BCs application, the agronomic properties were enhanced up to 6-18%, 18-38%, 17-46%, 13-45%, 15-42%, 22-55% and 34-57% for germination rate, shoot length, shoot biomass, spike length, spike biomass, grain biomass and root biomass respectively. The human health risks of PTEs were significantly (p ≤ 0.05) decreased (31-93%) from toxicity level to safe level (except for Mn and Cu), after the BCs application. Based on the current study, the BCs (especially 800BC5) were recommended for reducing bioaccumulation of PTEs in different parts of the wheat plant, increasing growth and yield of wheat crop and decreasing human health risks via consumption of wheat grains.

Effects of biochar amendment on bacterial and fungal communities in the reclaimed soil from a mining subsidence area.

Biochar amendment of soil is well known to improve soil fertility and microbial function. However, little is known about the effect of biochar addition to reclaimed soil in coal mining subsidence area on microbial community. A plant soil cultivation experiment was conducted with wheat grown and four treatments were included: P and K fertilizer (CK); NPK inorganic fertilizer (NPK); NPK inorganic fertilizer and straw (NPKS); and NPK inorganic fertilizer and biochar (NPKB). The results indicated that biochar amendment significantly increased the concentrations of NH4+-N, total N, and available P and K compared with the NPK. Biochar addition also significantly increased the grain yield and total biomass of wheat. Furthermore, biochar amendment treatment increased the absolute abundance and altered the community structure of soil bacteria and fungi in the reclaimed soil. Illumina MiSeq sequencing showed that the addition of biochar increased α-diversity of bacteria and relative abundances of Proteobacteria, Actinobacteria, and Bacteroidetes, whereas the relative abundance of Firmicutes were decreased by 61%. However, biochar addition did not change the relative abundance of dominant fungal phyla. Redundancy analysis (RDA) suggested that total N, available P, and K contents were the key factors correlated with changes in microbial community structure. Overall, our results suggest that biochar amendment in reclaimed soil in coal mine subsidence area could increase wheat yield and abundance and alter microbial community compositions.