Potentially toxic element contamination and health risk assessment in bottled mineral waters consumed in Turkey.

This study aimed to evaluate the human health risk posed by potentially toxic elements (PTEs) in 59 bottled mineral water (BMW) samples of 25 different brands marketed in Turkey. The concentrations of Ag, Al, As, Cd, Cr, Co, Cu, Fe, Mn, Ni, and Pb in the BMW samples were analyzed using an ICP-OES and compared with maximum allowable concentrations (MACs). A higher concentration of As than MAC was detected in five BMW samples. The concentrations of Cd, Pb, Cr, Ni, and Al in the majority of BMW samples are higher than the MACs. The human health risk was evaluated for adults estimating non-carcinogenic risk index (HI), incremental lifetime cancer risk (ILCR) and total cancer risk (TCR). All HI values estimated for PTEs in BMW samples are less than one, except for one sample. ILCR values for Cd in approximately half of the BMW samples are above the upper limit.

Measurement of radium and radon gas in bottled mineral waters.

In this study, the radon gas and radium concentration in the bottled mineral water samples was measured. A total of sixty samples were collected from bottled mineral water sold in the markets in Kahramanmaras. DURRIDGE Rad7 electronic radon detector was used for measurements. Radon and radium activity amounts range from 33.50 ± 1.30 mBq/L to 51.70 ± 2.20 mBq/L, and 2.92 ± 0.15 mBq/L to 4.51 ± 0.26 mBq/L, respectively. Average radon and radium active amounts are 41.67 ± 1.54 mBq/L and 3.63 ± 0.18 mBq/L, respectively. Total annual effective dose values were calculated for these mineral waters according to three different scenarios. In the first of these, it was assumed that natural mineral water was consumed annually instead of 730 L of drinking water (S1). In the second case, 150 L which was the annual average amount of natural mineral water consumed in European Union member countries was used for the annual average amount of natural mineral water consumed by adults (S2). Finally, the annual average amount of natural mineral water consumed in Turkey, 14 L, was used for adults (S3). For scenarios (S1, S2, S3), the total (ingestion + inhalation) annual average dose values ranged from 6.83E-04 mSv/y to 1.05E-03 mSv/y, 1.40E-04 mSv/y to 2.17E-04, and 1.32E-05 mSv/y to 2.03E-05 mSv/y, their average values were 8.49E-04 mSv/y, 1.75E-04 mSv/y, and 1.64E-05 mSv/y, respectively. The total effective dose values calculated within the scope of the current study were below the limit value announced by WHO.

Infectivity of Norovirus GI and GII from Bottled Mineral Water during a Waterborne Outbreak, Spain.

During a waterborne outbreak of norovirus in Spain, we estimated 50% illness doses for a group of exposed (secretor) persons to be 556 (95% CI 319-957) genome copies/day for norovirus GI and 2,934 (95% CI 1,683-5,044) genome copies/day for norovirus GII. Use of a propidium monoazide viability assay reduced these values.

Helicobacter pylori in bottled mineral water: genotyping and antimicrobial resistance properties.

BACKGROUND: Up to now, fecal-oral and oral-oral are the most commonly known routes for transmission of H. pylori, therefore, contaminated water can play an important role in transmission of H. pylori to humans. Genotyping using virulence markers of H. pylori is one of the best approaches to study the correlations between H. pylori isolates from different samples. The present research was carried out to study the vacA, cagA, cagE, oipA, iceA and babA2 genotyping and antimicrobial resistance properties of H. pylori isolated from the bottled mineral water samples of Iran. RESULTS: Of 450 samples studied, 8 samples (1.77%) were contaminated with H. pylori. Brand C of bottled mineral water had the highest prevalence of H. pylori (3.63%). The bottled mineral water samples of July month had the highest levels of H. pylori-contamination (50%). H. pylori strains had the highest levels of resistance against metronidazole (62.5%), erythromycin (62.5%), clarithromycin (62.5%), amoxicillin (62.5%) and trimethoprim (62.5%). Totally, 12.5% of strains were resistant to more than 6 antibiotics. VvacAs1a (100%), vacAm1a (87.5%), cagA (62.5%), iceA1 (62.5%), oipA (25%), babA2 (25%) and cagE (37.5%) were the most commonly detected genotypes. M1as1a (62.5%), m1as2 (37.5%), m2s2 (37.5%) and S1a/cagA+/IceA2/oipA-/babA2-/cagE- (50%) were the most commonly detected combined genotypes. CONCLUSIONS: Contaminated bottled mineral water maybe the sources of virulent and resistant strains H. pylori. Careful monitoring of bottled mineral water production may reduce the risk of H. pylori transmission into the human population.

Quality assessment of Romanian bottled mineral water and tap water.

This study reports the evaluation of bottled mineral water characteristics using fluorescence spectroscopy (synchronous fluorescence scans and emission spectra) and physico-chemical analyses. Samples from 14 still mineral water brands were compared to 11 tap waters collected from two Romanian cities. Correlation and factor analyses were undertaken to understand the relationships between the individual components. The concentration of major and minor ions showed great variation between the bottled mineral water samples highlighting the diversity of the water intakes, while in the case of tap water the chemical composition was relatively similar for samples collected in the same city. Fluorescence data showed that the mineral water contained low quantities of organic matter. The humic fraction was dominant in all samples, while the microbial fraction was low in most samples. Synchronous fluorescence scans provided more information, regarding the composition of organic matter, compared to emission spectra. The study evidenced the correlation between fluorescence parameters and major elements and highlighted the potential of using fluorescence for qualitative evaluation of the bottled mineral water quality, as a screening method before undertaking complex analyses.

Are bottled mineral waters and groundwater for human supply different?

Increasingly, bottled natural mineral water (NMW) is proposed as a healthy and safe alternative to supply water. However, tap supply water often comes from aquifers (TGW), even from the same aquifers as NMW, sharing the exact formation mechanisms and mineralization processes. Therefore, it is hypothesized that NMW and TGW cannot be distinguished. The chemical composition of TGW and NMW samples in Spain has been compared using five criteria: expert judgment, hydrochemistry, legal regulations, statistical analysis, and machine learning (ML). Hydrochemical criteria included all the NMW samples in the TGW group, as did the legal criterion, whereas classical statistical analysis could not find significant differences between the two groups. Although experts could correctly differentiate a small subsample of both types of water with an accuracy of 0.67, ML-based classification with Extreme Gradient Boosting yielded a balanced accuracy of 0.92 on an extremely imbalanced data set. Shapley Additive Explanations (SHAP) analysis identified pH, SiO2, E, K+, Ca2+, K+/Na+ and NO3- as the most relevant variables for water type discrimination. The overall consistency and generalization ability of the ML classifier has been proven by the spatial distribution of hits and misses, where the few cases of indistinguishable waters seem to be related to proximity to nature reserves (i.e., land use) more than to geological characteristics. Therefore, it can be concluded that NMW and TGW are indeed different and that only ML could find the hidden structure in the chemical data that determines the differences. This structure originates in how the market and consumers decide which water is ultimately bottled. The results can help on future choices of TGW and NMW in a context of water scarcity.

Disconfirmation of Taste as a Measure of Trust in Brands: An Experimental Study on Mineral Water.

The underlying factors of the purchase decision process of bottled mineral water have been a less studied area. The typically related attributes of consumer judgement in the case of low involvement can vary widely, ranging from price sensitivity to habits. However, assessing the role of brand reputation and trust from a sensory perception perspective is not a common approach. This paper examines the impact of trust on consumer value judgements for a frequently consumed beverage such as mineral water. Combining trust and sensory attributes with the Expectation-Disconfirmation Theory (EDT) framework provides insights into the weight of taste, trust and reputation in product evaluation. A tasting experiment was carried out using a representative systematic random sampling method. A mixed method was applied; EDT was used to analyze quantitative data and grounded theory methodology was performed in the case of qualitative data. Results indicate complete assimilation for the most preferred brand and negative contrast for less well-known brands. It can be stated that the applied methodology is suitable as a certain kind of trust measurement and also can function particularly well as a reinforcement and complement to other methodologies (e.g., neuromarketing methods). This study suggests that brand names positively influence value judgment. Origin bounded brands compared to imported brands can help companies mitigate trust issues in developing countries as they convey a particular reputation, which helps reinforce trust.

Comment on "exposure to microplastics (<10 µm) associated to plastic bottles mineral water consumption: The first quantitative study by Zuccarello et al. [Water Research 157 (2019) 365-371]".

Microplastics in food is a relatively new research field with only few studies available so far. Scientists have been pointing out that some of these studies apply questionable analytical methods. Nevertheless, media often use such results to gain attention of the readers. It is therefore of particular significance, that only those scientific studies are published, clearly presenting valid data on the content of microplastics in food. Unfortunately, the study by Zuccarello et al. shows very critical aspects regarding analytical methods used and conclusions made. The applied procedure is not described and, therefore, does not allow any assessment by other groups, which is indispensable prerequisite of any scientific publication. Moreover, the analytical method used for the identification and quantification of microplastic particles - SEM-EDX - is not sound and not validated. Therefore, in our opinion the results on the contamination of bottled mineral water with microplastics published by Zuccarello et al. are more than questionable.

Chemical composition of Chilean bottled waters: Anomalous values and possible effects on human health.

The Chilean bottled water market has experienced continuous growth since 2000, surpassing 500 million liters sold in 2015. Generally, consumers tend to associate the consumption of bottled water with a healthy lifestyle, but current Chilean law does not require the product to be labelled with the chemical composition, thus preventing consumers from making informed choices. Our study focuses on determining the water quality of ten brands of bottled water available for sale in Santiago, Chile. All of the analyzed water was not carbonated and in plastic containers in the 1.5 L size when available and the closest size to this when not available. Thirty-two chemical elements were analyzed, including minor and trace elements, and the data have been evaluated with respect to the limits established by Chilean and international regulatory agencies. Our results indicate that the quality of the analyzed water generally complies with Chilean law for bottled water. However, 30% of the analyzed samples exceed the values of arsenic (As) permitted by Chilean drinking water regulations, the World Health Organization and the United States Environmental Protection Agency. In 40% of the samples, the NO3 content is higher than groundwater values suggesting that the source of the bottled water is superficial. The purified bottled water brands contain minimal amounts of dissolved elements but do not comply with all of the parameters (e.g., pH) established by Chilean drinking water regulations. Our study highlights that there is an inconsistency between the Chilean norms that regulate bottled water and those that regulate drinking water. Some of the analyzed bottled waters do not comply with the drinking water regulations and paradoxically these brands should not be consumed by humans. However, risk assessment calculations for As ingestion show that the consumption of 1 L/day of bottled water does not pose a risk for human health.

Development of new analytical method for preconcentration/speciation of inorganic antimony in bottled mineral water using FIA-HG AAS system and SiO2/Al2O3/SnO2 ternary oxide.

In the present paper, a new analytical preconcentration/speciation method for antimony species determination in bottled mineral water samples using the SiO2/Al2O3/SnO2 adsorbent was developed. The method is based on selective adsorption of Sb(III) ions by SiO2/Al2O3/SnO2 under a wide pH range (2.5-7.5). Total antimony was determined with previous sample treatment using 0.1% (w/v) l-cysteine and the concentration of Sb(V) species was determined by the difference between total and Sb(III). The proposed method provided an analytical curve ranging from 0.50 to 5.00 µg L-1 (r = 0.999), limit of detection (LD) of 0.17 µg L-1 and preconcentration factor (PF) of 136-fold. The method exhibited tolerance to different metal ions and the accuracy was attested from addition and recovery tests (95.2-106.0%) in bottled mineral water samples using 2.0% (w/v) l-cysteine, as well as by analysis of certified material. Only Sb(III) species were determined in mineral water (0.54-1.04 µg L-1).

Small-sized microplastics and pigmented particles in bottled mineral water.

Up to now, only a few studies about microparticle contamination of bottled mineral water have been published. The smallest analysed particle size was 5 µm. However, due to toxicological reasons, especially microparticles smaller than 1.5 µm are critically discussed. Therefore, in the present study, 32 samples of bottled mineral water were investigated for contamination by microplastics, pigment and additive particles. Due to the application of aluminium coated polycarbonate membrane filters and micro-Raman spectroscopy, a lowest analysed particle size of 1 µm was achieved. Microplastics were found in water from all bottle types: in single use and reusable bottles made of poly(ethylene terephthalate) (PET) as well as in glass bottles. The amount of microplastics in mineral water varied from 2649 ±â€¯2857 per litre in single use PET bottles up to 6292 ±â€¯10521 per litre in glass bottles. While in plastic bottles, the predominant polymer type was PET; in glass bottles various polymers such as polyethylene or styrene-butadiene-copolymer were found. Hence, besides the packaging itself, other contamination sources have to be considered. Pigment particles were detected in high amounts in reusable, paper labelled bottles (195047 ±â€¯330810 pigment particles per litre in glass and 23594 ±â€¯25518 pigment particles per litre in reusable paper labelled PET bottles). Pigment types found in water samples were the same as used for label printing, indicating the bottle cleaning process as possible contamination route. Furthermore, on average 708 ±â€¯1024 particles per litre of the additive Tris(2,4-di-tert-butylphenyl)phosphite were found in reusable PET bottles. This additive might be leached out from the bottle material itself. Over 90% of the detected microplastics and pigment particles were smaller than 5 µm and thus not covered by previous studies. In summary, this is the first study reporting about microplastics, pigment and additive particles found in bottled mineral water samples with a smallest analysed particle size of 1 µm.