Antimicrobial activity of cleanser tablets against S. mutans and C. Albicans on different denture base materials.

BACKGROUND: In this study, the antimicrobial activity of three different cleanser tablets on S. mutans and C. albicans adhesion to PMMA, polyamide and 3D printed resin was investigated. METHODS: 40 samples were prepared for PMMA (SR Triplex Hot), polyamide (Deflex) and 3D printed resin (PowerResins Denture) materials and divided into four subgroups for cleansers (Aktident™, Protefix™, Corega™ tablets and distilled water) (n = 5). After the surface preparations were completed, the samples were immersed separately in tubes containing the prepared microorganism suspension and incubated at 37˚C for 24 h. After the incubation, the samples were kept in the cleanser solutions. The samples were then transferred to sterile saline tubes. All the tubes were vortexed and 10 µl was taken from each of them. Sheep blood agar was inoculated for colony counting. The inoculated plates were incubated for 48 h for S. mutans and 24 h for C. albicans. After incubation, colonies observed on all plates were counted. Statistical analyses were done with three-way ANOVA and Tukey's multiple comparison test. RESULTS: Polyamide material registered the highest colony count of S. mutans, whereas PMMA registered the lowest. Significant differences in S. mutans adherence (p = 0.002) were found between the three denture base materials, but no such difference in C. albicans adherence (p = 0.221) was identified between the specimens. All three cleanser tablets eliminated 98% of S. mutans from all the material groups. In all these groups, as well, the antifungal effect of Corega™ on C. albicans was significantly higher than those of the other two cleanser tablets. CONCLUSIONS: According to the study's results, it may be better to pay attention to surface smoothness when using polyamide material to prevent microorganism retention. Cleanser tablets are clinically recommended to help maintain hygiene in removable denture users, especially Corega tablets that are more effective on C. albicans.

Impact of effervescent denture cleaning tablets on color and surface characteristics of additively manufactured and hand-cast splint materials.

OBJECTIVES: This study is to evaluate the color stability, surface roughness, and hardness of additively manufactured and hand-cast splint materials after a 6-month commercially available denture cleaning tablet immersion simulation. MATERIALS AND METHODS: Disc-shaped additively manufactured and hand-cast auto polymerizing acrylic resin specimens were prepared (N = 40 each). All specimens were exposed to coffee solution totally 2 days. Thereafter, all specimens were immersed into three different effervescent solutions that simulated 6 months of clinical use. The total color change (ΔE*ab), surface roughness (Ra), and Vickers hardness (Vh) were measured at baseline and after immersion protocols. A two-way ANOVA and Bonferroni's post hoc test were used for color change. The dependent t-test and ANOVA were used for roughness and hardness evaluation. RESULTS: Additively manufactured splint materials were more affected by coffee immersion. It was observed that all denture cleaning tablets induced a noticeable alteration in color of the specimens (p < 0.05). Roughness and hardness measurement changes after solution immersions were statistically significant for both splint groups (p < 0.05). On the other hand, distilled water and denture cleaning tablets created similar roughness and hardness measurements at baseline and after immersion. CONCLUSIONS: After 6 months use of tested cleaning tables, the color stability, surface roughness, and hardness of both groups were affected. The evaluation of the surface properties of splint materials could be recommended to the dentists in periodic controls during splint treatments. CLINICAL RELEVANCE: The use effervescent denture cleaning tablets altered the surface characteristics of tested splint materials over time with nonsignificant difference between each other. After 6 months, awareness should be raised about surface characteristics of splint materials.

The effect of different denture cleansing solutions on the retention of polyetheretherketone locator attachments.

PURPOSE: Prosthetic cleansing solutions have been reported to cause retention loss in implant locator attachments due to the chemical alterations of these attachments. This study aimed to investigate the effects of different denture cleansing solutions on attachments with different retention values. MATERIALS AND METHODS: In this study, 160 polyetheretherketone locator attachments were divided into 4 groups according to their attachment retention values (yellow, pink, clear, and violet). Forty attachments from each group were soaked in four different cleansing solutions (n = 10). The attachments from the four groups were immersed in water, sodium hypochlorite, Corega, and Protefix solutions for a period equivalent to 6 months, according to the manufacturer's recommendations. The locator attachments were then subjected to testing using a universal testing machine. Maximum load to dislodgement according to different solutions was evaluated by one-way ANOVA test. Statistical significance was set at p < 0.05. RESULTS: The effect of denture cleansing solutions on the retentive values of attachments was statistically significant (p < 0.001). Among all cleansing solutions, the highest retention value was found in the violet attachments, and the lowest retention value was found in the yellow attachments. The lowest retention value (0.72 ± 0.05 MPa) was observed when the yellow attachments were immersed in Protefix solution, and the highest retention value (1.96 ± 0.15 MPa) was observed when the violet attachments were immersed in the Corega solution. CONCLUSION: For different colored attachments, different cleansing solutions can be suggested to patients.

Sargassum polycystum and Turbinaria conoides Seaweed-based Novel Denture Cleanser: An In Vitro Study.

AIM: The study ventures into evaluating the antifungal and antibacterial efficacy of commercially available denture cleanser with Sargassum polycystum, Turbinaria conoides seaweeds, and the combination of seaweeds. MATERIALS AND METHODS: Poly(methyl methacrylate) disks measuring 10 × 2 mm were fabricated. The samples are divided into four groups of 21 samples each. The denture base was coated with Candida albicans and Streptococcus mutans individually. Group I was treated with Fittydent, group II (S. polycystum and T. conoides seaweeds combination), group III (S. polycystum), and group IV (T. conoides). The colony-formation units present on the surface of the denture were evaluated before and after treatment with different denture cleansers using the serial dilution method. Statistical analysis was done using descriptive statistics, analysis of variance, and post hoc Bonferroni analysis. RESULTS: At 10-5 dilution, T. conoides (group IV) was statistically significant in reducing both C. albicans and S. mutans. At 10-10 dilution, T. conoides (group IV) and S. polycystum and T. conoides combination (group II) had high antibacterial efficacy and were statistically significant. Fittydent (group I) had higher antifungal efficacy and was statistically significant in comparison to S. polycystum (group III) alone. At 10-10 dilution, the T. conoides (group IV), S. polycystum, and T. conoides combination (group II) showed no evidence of a significant difference in comparison to Fittydent (group I). Fittydent had higher antibacterial efficacy and was statistically significant in comparison to S. polycystum (group III) alone. CONCLUSION: Sargassum polycystum and T. conoides combination and T. conoides were found to have higher antibacterial efficacy in comparison to commercially available denture cleanser and also were found to have equal antifungal efficacy in comparison to commercially available denture cleanser.

Persulfate-containing consumer products in the US market.

BACKGROUND: Persulfate compounds are reactive oxidative agents increasingly recognized as contact allergens. OBJECTIVES: The aim of this study was to identify common consumer products containing persulfate compounds in the US market. METHODS: Five publicly accessible online ingredient repositories and searches of two large online retailers were used to identify persulfate-containing consumer products. RESULTS: We identified persulfates in 23 hair colouring products, 11 denture cleansers, 8 pool/hot-tub products, 3 paints, and 3 cleaning products. CONCLUSIONS: Clinicians assessing contact dermatitis should be aware of three primary categories of consumer products that contain persulfates: denture cleansers, hair colouring products, and nonchlorine hot-tub and pool shock.

Effects of Denture Cleansers on the Flexural Strength of PMMA Denture Base Resin Modified with ZrO2 Nanoparticles.

PURPOSE: The additions of zirconium oxide nanoparticles (nano-ZrO2 ) to denture base materials have produced nanocomposites with satisfactory properties, although there is a lack of research investigating the effects of denture cleansers on these materials. This study aimed to evaluate the effect of denture cleansers on the flexural strength of denture base materials modified with nano-ZrO2 . MATERIALS AND METHODS: A total of 270 specimens were fabricated from pure and nano-ZrO2 reinforced acrylic resins at 2.5% and 5%, resulting in 3 main groups. The groups were further divided into subgroups (n = 10) according to immersion solution (distilled water, Corega, sodium hypochlorite, and Renew) and immersion duration. Flexural strength was measured at baseline (T0 ) in distilled water and after 180 and 365 days of immersion (T1 and T2 ) in denture cleansers. Data were collected and analyzed using repeated measure ANOVA and Bonferroni post hoc tests (α = 0.05). RESULTS: The flexural strength of the nano-ZrO2 modified denture base material decreased significantly after immersion in different denture cleansers at different immersion durations in comparison to baseline (T0 ) (p < 0.001). Sodium hypochlorite showed the highest reduction in flexural strength followed by Corega, while Renew cleansing solution resulted in the least change. CONCLUSION: Denture cleansers can significantly affect the flexural strength of nano-ZrO2 modified denture base materials and thus should be used cautiously.

Antifungal Effects of Herbal Extracts and Fluconazole on Heat-polymerized Acrylic Denture Base Resin as Denture Cleanser: An In Vitro Study.

AIM: The study aimed to investigate the antifungal effects of herbal extracts and fluconazole on heat-polymerized acrylic denture base resin as a denture cleanser. MATERIALS AND METHODS: Several essential oils, such as origanum oil and grape seed oil and commercially available antifungal agent fluconazole were used as denture cleansers and their antifungal efficacy was evaluated using a spectrophotometer. Overall, 68 samples were obtained and were divided into four groups, each containing 17 samples. These samples were immersed in Sabouraud dextrose broth consisting of Candida albicans for 16 hours and later in these antifungal solutions for 8 hours and their antifungal efficacy was measured. Data were subjected to an ANOVA test. RESULTS: Among the study groups origanum oil showed the maximum antifungal activity with a mean optical density at 0.072 ± 0.014 followed by fluconazole (0.094 ± 0.155), and least by grape seed oil (0.190 ± 0.071). CONCLUSION: Results of this in vitro study showed that origanum oil was more effective than commercially available antifungal agents, and among the tested groups oregano oil was a potential agent in lowering the C. albicans colony. CLINICAL SIGNIFICANCE: Origanum oil, being a herbal product, can be considered as a denture cleanser and also be used as an effective alternative to commercially available antifungal agents without any side effects.

Effect of Alkaline Peroxides on the Surface of Cobalt Chrome Alloy: An In Vitro Study.

PURPOSE: Removable denture hygiene care is very important for the longevity of the rehabilitation treatment; however, it is necessary to analyze the effects that denture cleansers can cause on the surfaces of prostheses. Thus, this study evaluated the effect of alkaline peroxide-effervescent tablets on the surface of cobalt-chromium alloys (Co-Cr) used in removable partial dentures. MATERIALS AND METHODS: Circular metallic specimens (12 × 3 mm) were fabricated and were immersed (n = 16) in: control, Polident 3 Minute (P3M), Steradent (S), Efferdent (E), Polident for Partials (PFP), and Corega Tabs (CT). The surface roughness (µm) (n = 10) was measured before and after periods of cleanser immersion corresponding to 0.5, 1, 2, 3, 4, and 5 years. Ion release was analyzed (n = 5) for Co, Cr, and molybdenum (Mo). Scanning electron microscopy (SEM) analysis and an Energy-dispersive X-ray spectroscopy (EDS) were conducted in one specimen. The surface roughness data were statistically analyzed (α = 0.05) with the Kruskal-Wallis test to compare the solutions, and the Friedman test compared the immersion durations. Ion release analysis was performed using 2-way ANOVA and Tukey's test. RESULTS: There was no significant surface roughness difference when comparing the solutions (p > 0.05) and the immersion durations (p = 0.137). Regarding ion release (µg/L), CT, E, and control produced a greater release of Co ions than S (p < 0.05). CT produced a greater release of Cr ions than control, S, and P3M (p < 0.05). Finally, E caused the greatest release of Mo ions (p < 0.05). SEM confirmed that the solutions did not damage the surfaces and EDS confirmed that there were no signs of oxidation. CONCLUSION: The various solutions tested did not have any deleterious effects on the Co-Cr alloy surface. Steradent, however, presented the smallest ionic release.

Denture Hygiene Knowledge and Practices among Complete Denture Wearers attending a Postgraduate Dental Institute.

AIMS: The aim of this study was to assess the denture hygiene knowledge and practices among patients using complete dentures attending a postgraduate dental hospital in Jabalpur city. The objective of this study was to assess the relationship between hygiene knowledge and practices to the denture wearer's gender, education, and income. MATERIALS AND METHODS: A descriptive, cross-sectional survey was conducted using a self-administered questionnaire involving the complete denture patients attending the Department of Prosthodontics. The study subjects were randomly selected by recruiting old dentures wearers visiting the Department of Prosthodontics for a recall visit or for new dentures on the odd dates of the month. All subjects signed an informed consent before filling the questionnaire. The institutional review committee approved the study. Descriptive statistics included computation of frequencies and percentages. Nonparametric test, namely, chi-square test, was used for further data analysis; p-value <0.05 was considered statistically significant. RESULTS: A total of 530 individuals were approached for participation in our survey, 30 (5.66%) subjects declined to participate. The results showed that 195 (39%) subjects were wearing the same dentures for more than 5 years. In this study, 51 (10.2%) subjects reported never having been advised by their dentists as to how to clean their dentures. Among all the subjects interviewed, 264 (52.8%) reported to clean the oral tissues daily. This study disclosed that 66 (13.2%) of the subjects usually slept with their dentures. Maximum subjects in illiterate group had experienced bad breath sometimes when compared with subjects in postgraduate group (χ2 = 47.452, p < 0.001). There was a significant difference for method of denture cleaning according to gender (χ2 = 101.076, p < 0.001). CONCLUSION: The majority of the denture wearers have limited knowledge of denture cleansing and oral hygiene practices. Hygiene habits and practices may not always present a positive correlation with the gender, educational level, and income of the subjects. CLINICAL SIGNIFICANCE: Periodic recall for evaluation of denture and mucosal surfaces along with reinforcement of denture hygiene instructions will go a long way in helping the patients reap maximum benefits out of their prostheses.

Evaluating the efficiency of humic acid to remove micro-organisms from denture base material.

OBJECTIVE: To evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials. BACKGROUND: Old denture wearer needs effective, easy-use and safe denture-cleaning material. MATERIAL AND METHODS: Square-shaped, heat-polymerised acrylic resin specimens (n = 550) were prepared and divided into five groups (n = 110 for each) corresponding to the microbial contamination (Candida albicans, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa). Contaminated specimens were randomly assigned to the application of five different denture-cleaning agents as follows (n = 20 for each): Kloroben, Corsodyl, Steradent, Corega, experimental solution with humic acid. Ten specimens were assessed as an experimental control carried out simultaneously for the treatment groups for each micro-organism. It was divided into two groups: negative control and positive control (n = 5 for each). All acrylic specimens were incubated 37°C for 24 h (for bacterial strains) and 37°C for 48 h (for yeast strains). After incubation period, all brain-heart infusion broths (BHI) which contain disinfectant acrylic specimens were cultured on 5% sheep blood agar (for bacteria) and Sabouraud dextrose agar (SDA) for yeast using loop. The numbers of colony-forming units per millilitre (CFU/ml) were calculated. The results were analysed by Mann-Whitney U-test and Kruskal-Wallis tests (p = 0.05). RESULTS: Corsodyl and Kloroben completely eliminated the adherence of all investigated micro-organisms (100%) and showed the highest removal activity compared with other cleaning agents (p < 0.05). There was no statistically significant difference between Corsodyl and Kloroben (p ≥ 0.05), and there was no statistically significant difference between Corega, Steradent and experimental solution (p ≥ 0.05). CONCLUSION: Humic acid could be used as an alternative 'natural' solution for denture-cleaning agent.

Variations in Flexural Strength of Heat-polymerized Acrylic Resin after the Usage of Denture Cleansers.

OBJECTIVE: The objective of this study was to evaluate and compare changes in the flexural strength of heat-cured denture base resins when treated using denture cleansers. STUDY DESIGN: A total of 40 specimens with dimension 65 mm length, 10 mm width, and 3 mm thickness were prepared as per ISO 1567 specification. A total of 10 specimens were immersed in distilled water to be used as control. Of the remaining 30 samples, 10 were treated with Clinsodent, 10 with VI-Clean, and 10 with Clanden denture cleansers. Specimens in each group were subjected to three-point flexural load in universal testing machine at a cross-head speed of 5 mm/min. The peak load (N) was recorded and flexural strength was calculated. The findings were analyzed using Kruskal-Wallis analysis of variance and Mann-Whitney test. RESULTS: Heat-cured denture base resin selected for this study showed significant difference in flexural strength after immersion in denture cleansers Clinsodent, VI-Clean, and Clanden solutions, when compared with the control group. CONCLUSION: Findings of this study showed that denture cleansers altered the flexural strength of heat polymerized acrylic resins that endured soaking cycles which simulated 180 days of use. Hence, denture cleansers should be used with caution, once a day after brushing the dentures. It is advisable for patients to follow the manufacturer's instructions.

Evaluation of sodium hypochlorite as a denture cleanser: a clinical study.

OBJECTIVES: To evaluate the effect of sodium hypochlorite (NaOCl) on biofilms, colour stability (ΔE) and surface roughness (Ra) of complete dentures and patient acceptability. BACKGROUND: Denture cleansers should be able to reduce the accumulation of biofilms without affecting the acrylic resin properties. Patient satisfaction is important to maintaining their daily use. MATERIALS AND METHODS: Fifteen participants were instructed to keep their dentures immersed daily in a 0.5% NaOCl solution for 3 min over 90 days. Swabs were taken from dentures and inoculated on CHROMagar and blood agar. The number of colony-forming units (cfu) was counted after a 48-h incubation period. ΔE was assessed using the CIE L*a*b* system. Ra was measured using a profilometer. Patient acceptability was checked based on their degree of satisfaction. Cell counts, ΔE and Ra were analysed using anova, Friedman's and Kruskal-Wallis tests, respectively (α = 0.05). RESULTS: A significant reduction in the total number of microorganisms (p = 0.001) and Candida spp. was noticed. No significant differences were found for ΔE (p = 0.68) and for Ra (p = 0.47). The level of the patient satisfaction increased throughout the follow-up period. CONCLUSION: The 0.5% NaOCl solution was effective in reducing microorganisms without significant changes in colour or roughness of denture resin. The participants reported satisfaction with the cleaning results.

Survival of microorganisms on complete dentures following ultrasonic cleaning combined with immersion in peroxide-based cleanser solution.

OBJECTIVES: To compare ultrasonic cleaning combined with immersion in a commercially available peroxide-based cleanser solution (Polident(®) ) with other denture cleaning methods, we examined the quantity of micro-organisms that survived on dentures before and after various cleaning methods. SUBJECTS AND METHODS: One hundred complete dentures belonging to 50 nursing home residents (mean age, 84.6 years) were randomly assigned to five groups according to the cleaning method employed: (A) immersion in Polident(®) solution alone, (B) brushing with water, (C) ultrasonic cleaning with water, (D) method (A) followed by method (B) and (E) ultrasonic cleaning combined with immersion in Polident(®) solution. Before and after the dentures had been cleaned, denture biofilm was collected from the mucosal surface of each lateral half of the examined dentures. The collected micro-organisms were cultured, presumptively identified by standard methods and quantified. Comparisons between the five cleaning methods were carried out using the Kruskal-Wallis test and Dunn's multiple comparisons test. RESULTS: The denture cleaning methods involving the use of Polident(®) solution (methods A, D and E) were significantly more effective at denture disinfection than the other methods (p < 0.05); in particular, the quantity of Candida spp. was lowest after method E (median, 0.00; significantly lower than those observed after methods A, B and C; p < 0.05). CONCLUSION: It was concluded that ultrasonic cleaning combined with immersion in a peroxide-based cleanser solution effectively reduces the quantity of micro-organisms surviving on dentures and is a suitable method for elderly individuals who find brushing their dentures difficult.

Evaluation of awareness and knowledge about denture cleansers among dental professionals.

Denture cleansers have been widely used in prosthodontics for cleaning and maintaining dentures. However, little is known about dentist's knowledge, awareness and experience of denture cleansers. Denture wearers would benefit greatly from a dental professional's guidance regarding use of dental cleansers. Basic objective of this study is to investigate awareness and knowledge about denture cleansers among dental professionals. A quota sampling technique was adopted with a sample size of 200 respondents. A well-structured questionnaire was used for collection of cross-sectional data. Statistical analyses were adopted to draw logical and scientific inferences. The χ (2) test was used to test the independence of various attributes. Highest number of respondents (55.5 %) used tablet form of denture cleanser. 47.5 % reported that they had little knowledge about adverse effects of denture cleansers. A significant percentage (36 %) reported that no knowledge is imparted about denture cleansers in their curriculum. Eighty seven percent of respondents felt the need to enhance their knowledge. It is inferred from the present study that it is very important to raise dentist's awareness and knowledge about denture cleansers by teaching them in more depth and further in continuing education and training, so as to enable them to impart adequate guidance to their patients.

Effectiveness of Denture Cleansers on Candida albicans Biofilm on Conventionally Fabricated, Computer-Aided Design/Computer-Aided Manufacturing-Milled, and Rapid-Prototyped Denture Base Resins: An In Vitro Study.

INTRODUCTION: Conventionally fabricated denture base resins have been used for over 150 years. Newer denture base resins can provide a superior fit and may be customized to the patient's characteristics, but the literature on their cleansibility remains limited. The oral cavity can be a hub for thousands of microflora. The maintenance of complete dentures by edentulous patients depends not only on the maintenance of the patient but also on the material used, biofilm adherence, and polishability. MATERIALS AND METHODS: Cuboid specimens of 10 × 5 × 2 mm were designed using the Meshmixer version 3.5 software (Meshmixer, Australia). The standard tessellation (STL) file was imported and sent for printing (NextDent, Netherlands) (Group 1), milling in polymethyl methacrylate (PMMA) (Ivotion, Ivoclar, Schaan, Liechtenstein) (Group 2), and wax milling (Upcera, China), followed by flasking, counter flasking, and packing using heat-cured acrylic resin (DPI, India) (Group 3). The obtained specimens were polished using pumice and sterilized using a UV sterilization unit. The specimens were then immersed in a suspension of candida broth. After three days of biofilm formation, a colony count was performed and noted as colony-forming units per milliliter (CFU/mL). Specimens were treated using Secure denture cleansing tablets (Ghent, New York), table salt (iodized table salt, Tata, India), Clinsodent (ICPA, Mumbai, India), and Polident denture cleansing powder (Polident, Ontario, Canada). A colony count was done after treatment, and the data were tabulated. Statistical analysis was done using SPSS software to compare the efficiency of denture cleansers in all three groups, and statistical significance was set at 0.05. The Kolmogorov-Smirnov test was done to confirm the normality of the data, followed by a one-way analysis of variance (ANOVA) test to compare the efficiency of denture cleansers on the removal of candida colonies. RESULTS: Milled denture base resins showed a significantly lower colony count when compared to printed and conventionally fabricated denture base resins. The denture cleansers showed high efficacy in all groups, with the most significant being Secure, which showed a mean difference ranging from 8.114 to 9.887 CFU/mL, followed by Clinsodent, showing a mean of 6.699-9.863 CFU/mL, followed closely by Polident, showing 4.964-7.114 CFU/mL, followed by table salt, being 5.254-8.920 CFU/mL. The 95% confidence interval confirmed statistical significance. CONCLUSION: The highest candida colony count was demonstrated by the conventional, followed by rapid prototyping, and was least with milled denture base resins. Following treatment with denture cleansers, Secure demonstrated almost complete eradication of colonies, making it the most effective option. Salt exhibited the lowest efficiency, followed closely by Polident and Clinsodent, and the most effective was Secure denture cleanser.

Anti-Microbial Effect of AgBr-NP@CTMAB on Streptococcus Mutans and Assessment of Surface Roughness Hardness and Flexural Strength of PMMA.

Purpose: To investigate the inhibition of Streptococcus mutans (S.mutans) and its biofilm by AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) and evaluate the changes in Polymethyl methacrylate (PMMA)'s surface roughness (Ra), microhardness, and flexural strength during prolonged immersion in AgBr-NP@CTMAB for application in the denture cleaning industry. Patients and Methods: The antibacterial activity of AgBr-NP@CTMAB against S.mutans was measured colony formation assay, OD600 and laser confocal microscopy. Changes in the specimens' values for surface roughness, microhardness, and flexural strength (MPa) were measured after immersion solutions for 180 or 360 days. Results: The AgBr-NP@CTMAB solution exhibited a robust antibacterial effect on planktonic S. mutans, with a minimum bactericidal concentration of 5 µg/mL. The 10 µg/mL AgBr-NP@CTMAB solution efficiently inhibited S. mutans biofilm formation. (2) No significant difference in surface roughness after immersion in AgBr-NP@CTMAB (10 µg/mL and 20 µg/mL) comparing with distilled water (P > 0.05) and Polident had significantly higher than distilled water (P < 0.05). There was a significant decrease in the surface hardness of the PMMA specimens that were immersed in the Polident compared with those in distilled water (P < 0.05). While, no significant differences in surface hardness after immersion in the AgBr-NP@CTMAB (P > 0.05). The result of flexural strength suggested that there was no statistically significant difference (P < 0.05) between AgBr-NP@CTMAB as well as Polident and water. Conclusion: AgBrNP@CTMAB can efficiently inhibit the growth of plankton S.mutans and biofilm formation, without affecting the flexural strength, microhardness, or surface roughness of PMMA. Therefore, AgBrNP@CTMAB holds promise as a new denture cleaning agent.

Aging processes in dental thermoplastics - Thermoanalytical investigations and effects on Vickers as well as Martens hardness.

OBJECTIVE: The influence of various aging protocols, representing and accelerating influences present in the dental context, on possible changes in the microstructure and mechanical properties of thermoplastics was investigated. In order to minimize the complexity of the systems, first pure polymers and then later the equivalent dental polymeric materials were analyzed. MATERIALS AND METHODS: Pure polymers (Poly(methyl methacrylate) - PMMA, Polyoxymethylene homopolymer - POM-H, Polyether ether ketone - PEEK, Nylon 12 - PA12, Polypropylene - PP) were analyzed before as well as after applying different aging protocols relevant to the oral environment (ethanol, thermocycling, alkaline and acidic setting) by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The thermoanalytical parameters used were glass transition temperature (Tg), melting peak and crystallization peak temperature (Tpm, Tpc) and decomposition behavior. In a second step selected commercially available dental products (Telio CAD - PMMAD, Zirlux Acetal - POMD, Juvora Natural Dental Disc - PEEKD) aged by the protocol that previously showed strong effects were examined and additionally tested for changes in their Vickers and Martens hardness by Mann-Whitney-U test. RESULTS: The combinations of pure polymers and viable aging protocols analyzed within this study were identified via TGA or DSC as PA12 & thermocycling, POM-H & denture cleanser/lactic acid/ethanol, PP & lactic acid. The dental polymeric materials PMMAD and POMD due to aging in lactic acid showed slight but significantly (p < 0.01) reduced Vickers and partly Martens hardness. PEEK showed the greatest material resistance within this study.

Effect of cigarette smoke and denture cleansers on the surface properties and color stability of CAD-CAM and conventional denture base resins.

This study aimed to evaluate the hardness, roughness, and color stability of heat- and microwave-polymerized polymethyl methacrylate (PMMA), polyamide, and CAD-CAM PMMA resins when exposed to cigarette smoke (CS) and immersed in a denture cleanser (DC). Specimens of each resins were divided into four subgroups (n=10). The DC and CS+DC specimens were immersed in a DC; the CS and CS+DC specimens were exposed to CS; and the control (C) specimens were kept in distilled water. Hardness, roughness, and color measurements were performed. DC and CS significantly affected the roughness and color of all resins (p<0.05). However, these did not affect the hardness of the CAD-CAM PMMA resin (p>0.05). The CAD-CAM PMMA resin presented the lowest roughness, highest hardness, and lowest ΔE00 values. While the roughness of all resins exposed to CS when immersed in DC decreased, the ΔE00 values of the conventional PMMAs also decreased. Although this decreases were not significant, it may be advisable to use DC for smokers to decrease discoloration and roughness especially for conventional PMMAs resins.

Comparison of antifungal efficacy of commercially available denture cleanser and the extract of Turbinaria conoides seaweed against Candida albicans adherent to acrylic denture base resin: An in vitro study.

Aim: One of the main factors responsible for the development of Candida albicans on the surface of denture is improper maintenance of dentures. Denture hygiene can be achieved by regular cleansing of dentures using an appropriate denture cleanser. The aim of the study is to evaluate the antifungal efficacy of commercially available denture cleanser and the extract of Turbinaria conoides seaweed against C. albicans adherent to the surface of denture base resin. Settings and Design: This was an in vitro experimental study. Materials and Methods: Twenty-four Acrylic resin samples of dimension 10-mm radius and 2-mm thickness was randomly divided into two groups. The denture base resin was coated with C. albicans. The colonies present on the surface of each denture base resin were evaluated by serial dilution method. Group A was treated with commercially available denture cleanser and Group B was treated with extract of seaweed T. conoides. The colonies were then evaluated using serial dilution. Statistical Analysis Used: The colony count values obtained by serial dilution were tabulated. These values were statistically analysed using t-test. Results: Reduction of colony count is more in T. conoides than commercially available Fittydent; the difference was statistically significant with a mean difference of 65 at dilution 10-2 and 29.25 at dilution 10-3 using t-test with P < 0.001. Conclusion: Within the limitations of this in vitro study, it was proved that the extract of T. conoides seaweed and commercially available denture cleanser Fittydent was effective in reducing the colony count of C. albicans. T. conoides seaweed is statistically significant than commercially available Fittydent.

The Influence of Cleaning Solutions on the Retention of Overdenture Attachment Systems.

Dental-implant-supported reconstructions provide comfort and improvements in prosthetic function, adaptation, and stability over conventional treatment options. The objective of this study was to evaluate the effect of different denture cleansing solutions and their influence on the deterioration and loss of retention of overdenture attachments in a 12-month clinical-use simulation. In this way, ten specimens each of different brands of retentive caps made of Teflon (OT Equator® (Rhein83, Bologna, Italy), Locator® (Zest Anchors, Escondido, CA, USA), Kerator® (KJ Meditech, Gwangiu, Republic of Korea), and Locator R-Tx® (Zest Anchors, Escondido, CA, USA)) were immersed in five different cleaning solutions (Kukident® (P&G Tech, Oxford Parkway, UK), Benfix® (Laboratorios URGO S.L., Guipúzcoa, Spain), Corega® (Stafford Miller, Waterford, Ireland), and Protefix® (Neuhofer Weiche, Parchim, Germany)), and tap water was used as the control group, in a simulation that lasted 12 months. Data were analyzed using two-way ANOVA and a Tukey HSD. Furthermore, a Levene Test and Shapiro-Wilk tests were performed to assess the validation of the ANOVA assumptions. The statistical analysis was performed using R version 4.2.2 software with the significance level set to p < 0.05. There were significant statistical differences between the different manufacturers regarding the retention forces of the attachment's retentive caps (F = 322.066, p < 0.001). For the cleaning solution groups, different statistical results between Kukident® (P&G Tech, Oxford Parkway, UK) (p < 0.05) and Benfix® (Laboratorios URGO S.L., Guipúzcoa, Spain) (p < 0.05) were observed. There were no significant statistical differences between Corega® (Stafford Miller, Ireland), Protefix® (Neuhofer Weiche, Parchim, Germany), and tap water, even though the retention forces decreased in all of them.